{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:24:22Z","timestamp":1771262662052,"version":"3.50.1"},"reference-count":66,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T00:00:00Z","timestamp":1592524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["CESAM UIDP\/50017\/2020"],"award-info":[{"award-number":["CESAM UIDP\/50017\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["CESAM UIDB\/50017\/2020"],"award-info":[{"award-number":["CESAM UIDB\/50017\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["BioISI UIDB\/04046\/2020"],"award-info":[{"award-number":["BioISI UIDB\/04046\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["cE3c UIDB\/00329\/2020"],"award-info":[{"award-number":["cE3c UIDB\/00329\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["PCIF\/GVB\/0202\/2017"],"award-info":[{"award-number":["PCIF\/GVB\/0202\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diversity"],"abstract":"<jats:p>Acacia longifolia is a worldwide invader that cause damage in ecosystems, expanding largely after wildfires, which promote germination of a massive seed bank. As a legume, symbiosis is determinant for adaptation. Our study aims to isolate a wider consortium of bacteria harboured in nodules, including both nitrogen and non-nitrogen fixers. Furthermore, we aim to evaluate the effects of fire in nodulation and bacterial diversity on young acacias growing in unburnt and burnt zones, one year after the fire. For this, we used molecular approaches, M13 fingerprinting and 16S rRNA partial sequencing, to identify species\/genera involved and \u03b415N isotopic composition in leaves and plant nodules. Nitrogen isotopic analyses in leaves suggest that in unburnt zones, nitrogen fixation contributes more to plant nitrogen content. Overall, A. longifolia seems to be promiscuous and despite Bradyrhizobium spp. dominance, Paraburkholderia spp. followed by Pseudomonas spp. was also found. Several species not previously reported as nitrogen-fixers were identified, proposing other functions besides ammonia acquisition. Our study shows that bacterial communities are different in nodules after fire. Fire seems to potentiate nodulation and drives symbiosis towards nitrogen-fixers. Taken together, a multifunctional community inside nodules is pointed out which potentiate A. longifolia invasiveness and adaptation.<\/jats:p>","DOI":"10.3390\/d12060250","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T10:43:58Z","timestamp":1592563438000},"page":"250","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Acacia longifolia: A Host of Many Guests Even after Fire"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8007-5031","authenticated-orcid":false,"given":"Joana Guedes de","family":"Jesus","sequence":"first","affiliation":[{"name":"Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ci\u00eancias da Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Centro de Estudos do Ambiente e do Mar (CESAM), Faculdade de Ci\u00eancias da Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3097-3236","authenticated-orcid":false,"given":"Rog\u00e9rio","family":"Tenreiro","sequence":"additional","affiliation":[{"name":"Biosystems and Integrative Sciences Institute (BioISI), Faculdade de Ci\u00eancias da Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Cristina","family":"M\u00e1guas","sequence":"additional","affiliation":[{"name":"Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ci\u00eancias da Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Helena","family":"Trindade","sequence":"additional","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM), Faculdade de Ci\u00eancias da Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/474153a","article-title":"Don\u2019t judge species on their origins","volume":"474","author":"Davis","year":"2011","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.0022-0477.2004.00953.x","article-title":"A biogeographical approach to plant invasions: The importance of studying exotics in their introduced and native range","volume":"93","author":"Hierro","year":"2005","journal-title":"J. Ecol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/B978-0-12-384719-5.00319-1","article-title":"Plant Invasions","volume":"6","author":"Richardson","year":"2013","journal-title":"Encycl. Biodivers."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"16112","DOI":"10.1038\/nplants.2016.112","article-title":"Neglecting legumes has com- promised human health and sustainable food production","volume":"2","author":"Foyer","year":"2016","journal-title":"Nat. Plants"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1071\/SB02008","article-title":"Overview of the generic status of Acacia (Leguminosae: Mimosoideae)","volume":"16","author":"Maslin","year":"2003","journal-title":"Aust. Syst. Bot."},{"key":"ref_6","unstructured":"Wollman, D.J. (1980). Acacias of South Australia, Government Printer."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s13595-017-0651-0","article-title":"Here to stay. Recent advances and perspectives about Acacia invasion in Mediterranean areas","volume":"74","author":"Alonso","year":"2017","journal-title":"Ann. For. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1071\/FP04197","article-title":"Phenotypic plasticity of an invasive acacia versus two native Mediterranean species","volume":"32","author":"Peperkorn","year":"2005","journal-title":"Funct. Plant Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jnc.2016.11.001","article-title":"Big troubles are already here: Risk assessment protocol shows high risk of many alien plants present in Portugal","volume":"35","author":"Morais","year":"2017","journal-title":"J. Nat. Conserv."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/BF00328825","article-title":"Impacts of invading N2-fixing Acacia species on patterns of nutrient cycling in two Cape ecosystems: Evidence from soil incubation studies and 15N natural abundance values","volume":"101","author":"Stock","year":"1995","journal-title":"Oecologia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1614\/0890-037X(2004)018[1427:RPODEI]2.0.CO;2","article-title":"Recovery Potential of Dune Ecosystems Invaded by an Exotic Acacia Species (Acacia longifolia)1","volume":"18","author":"Marchante","year":"2004","journal-title":"Weed Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s10530-006-0008-3","article-title":"Functional Group Identity Does not Predict Invader Impacts: Differential Effects of Nitrogen-fixing Exotic Plants on Ecosystem Function","volume":"9","author":"Yelenik","year":"2006","journal-title":"Biol. Invasions"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1111\/j.1600-0706.2009.18148.x","article-title":"Disturbance influences the outcome of plant-soil biota interactions in the invasive Acacia longifolia and in native species","volume":"119","author":"Carvalho","year":"2010","journal-title":"Oikos"},{"key":"ref_14","first-page":"231","article-title":"N\/P imbalance as a key driver for the invasion of oligothrophic dune systems by a woody legume","volume":"126","author":"Ulm","year":"2016","journal-title":"Oikos"},{"key":"ref_15","first-page":"1567","article-title":"Early Acacia invasion in a sandy ecosystem enables shading mediated by soil, leaf nitrogen and facilitation","volume":"20","author":"Tolentino","year":"2017","journal-title":"Biol. Invasions"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Andrews, M., and Andrews, M. (2017). Specificity in Legume-Rhizobia Symbioses. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18040705"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s00248-018-1214-0","article-title":"The Functional Potential of the Rhizospheric Microbiome of an Invasive Tree Species, Acacia dealbata","volume":"77","author":"Kamutando","year":"2018","journal-title":"Microb. Ecol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1111\/j.1365-2699.2010.02284.x","article-title":"Rhizobial hitchhikers from Down Under: Invasional meltdown in a plant-bacteria mutualism?","volume":"37","year":"2010","journal-title":"J. Biogeogr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1128\/AEM.67.1.396-402.2001","article-title":"Small-Subunit rRNA Genotyping of Rhizobia Nodulating Australian Acacia spp","volume":"67","author":"Lafay","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/S0038-0717(99)00032-2","article-title":"Identification and characterization of fast- and slow-growing root nodule bacteria from South-Western Australian soils able to nodulate Acacia saligna","volume":"31","author":"Marsudi","year":"1999","journal-title":"Soil Biol. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1099\/00207713-49-4-1359","article-title":"Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya","volume":"49","author":"Nick","year":"1999","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0723-2020(99)80076-9","article-title":"Rhizobia isolated from root nodules of tropical leguminous trees characterized using DNA-DNA dot-blot hybridization and rep-PCR genomic fingerprinting","volume":"22","author":"Nick","year":"1999","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5066","DOI":"10.1128\/AEM.00613-07","article-title":"Genetic Diversity of Rhizobia Associated with Acacia longifolia in Two Stages of Invasion of Coastal Sand Dunes","volume":"73","author":"Crisostomo","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/j.1472-4642.2011.00787.x","article-title":"Jack-of-all-trades and master of many? How does associated rhizobial diversity influence the colonization success of Australian Acacia species?","volume":"17","author":"Crisostomo","year":"2011","journal-title":"Divers. Distrib."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1442-9993.1984.tb01348.x","article-title":"Seed characteristics and regeneration of some species in invaded coastal communities","volume":"9","author":"Weiss","year":"1984","journal-title":"Austral Ecol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1094\/PBIOMES-12-16-0019-RVW","article-title":"The Nodule Microbiome: N2-Fixing Rhizobia Do Not Live Alone","volume":"1","author":"Hirsch","year":"2017","journal-title":"Phytobiomes J."},{"key":"ref_27","unstructured":"(2020, April 30). Boletim climatol\u00f3gico 2018. Available online: http:\/\/www.ipma.pt\/pt\/publicacoes\/boletins."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s11104-016-2838-9","article-title":"Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India","volume":"410","author":"Sankhla","year":"2016","journal-title":"Plant Soil"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"977","DOI":"10.2307\/2402718","article-title":"A Manual for the Practical Study of Root-Nodule Bacteria","volume":"8","author":"Callow","year":"1971","journal-title":"J. Appl. Ecol."},{"key":"ref_30","unstructured":"Cappuccino, J., and Sherman, N. (1998). Experiment 2, Techniques for Isolation of Pure Cultures. Microbiology, A Laboratory Manual, Benjamin-Cummings Pub Co. [5th ed.]."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.apsoil.2012.10.005","article-title":"Co-introduction of exotic rhizobia to the rhizosphere of the invasive legume Acacia saligna, an intercontinental study","volume":"64","author":"Crisostomo","year":"2013","journal-title":"Appl. Soil Ecol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.1472-765X.1989.tb00262.x","article-title":"Rapid extraction of bacterial genomic DNA with guanidium thiocyanate","volume":"8","author":"Pitcher","year":"1989","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1126\/science.2880398","article-title":"A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA","volume":"235","author":"Vassart","year":"1987","journal-title":"Science"},{"key":"ref_34","unstructured":"Krebs, C. (1989). Ecological Methodology, Harper Collins."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1128\/AEM.64.2.795-799.1998","article-title":"Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA","volume":"64","author":"Marchesi","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_36","unstructured":"(2019, April 03). Geneious v5.3. Available online: http:\/\/www.geneious.com."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0378-1127(92)90011-W","article-title":"Soil mineral nitrogen changes following prescribed burning in ponderosa pine","volume":"54","author":"Covington","year":"1992","journal-title":"For. Ecol. Manag."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plantsci.2014.08.014","article-title":"Nitrate inhibits soybean nodulation by regulating expression of CLE genes","volume":"229","author":"Lim","year":"2014","journal-title":"Plant Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1111\/j.1469-8137.2010.03542.x","article-title":"Relationship between gibberellin, ethylene and nodulation in Pisum sativum","volume":"189","author":"Ferguson","year":"2010","journal-title":"New Phytol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/07352688809382257","article-title":"Inhibition of legume nodule formation and N2fixation by nitrate","volume":"7","author":"Streeter","year":"1988","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1093\/jexbot\/53.368.423","article-title":"Short-term metabolic responses of soybean root nodules to nitrate","volume":"53","author":"Gordon","year":"2002","journal-title":"J. Exp. Bot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"255","DOI":"10.2135\/cropsci1974.0011183X001400020026x","article-title":"Soil and symbiotic nitrogen requirements for optimum production of soybean","volume":"14","author":"Harper","year":"1974","journal-title":"Crop Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1104\/pp.76.3.743","article-title":"Increase of Natural 15N Enrichment of Soybean Nodules with Mean Nodule Mass","volume":"76","author":"Shearer","year":"1984","journal-title":"Plant Physiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1007\/s004420050535","article-title":"Vascular plant 15 N natural abundance in heath and forest tundra ecosystems is closely correlated with presence and type of mycorrhizal fungi in roots","volume":"115","author":"Michelsen","year":"1998","journal-title":"Oecologia"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s11104-012-1421-2","article-title":"The dual symbiosis between arbuscular mycorrhiza and nitrogen fixing bacteria benefits the growth and nutrition of the woody invasive legume Acacia cyclops under nutrient limiting conditions","volume":"366","author":"Mortimer","year":"2012","journal-title":"Plant Soil"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1104\/pp.84.3.835","article-title":"Investigation of the Role of Phosphorus in Symbiotic Dinitrogen Fixation","volume":"84","author":"Israel","year":"1987","journal-title":"Plant Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1093\/aob\/mcf260","article-title":"The Influence of Phosphorus Deficiency on Growth and Nitrogen Fixation of White Clover Plants","volume":"90","author":"Jensen","year":"2002","journal-title":"Ann. Bot."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Taylor, B., Simms, E.L., and La Pierre, K.J. (2020). More Than a Functional Group: Diversity within the Legume\u2013Rhizobia Mutualism and Its Relationship with Ecosystem Function. Diversity, 12.","DOI":"10.3390\/d12020050"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1071\/BT9850595","article-title":"Biological Nitrogen Fixation and Root-Nodule Bacteria (Rhizobium Sp. and Bradyrhizobium Sp.) In Two Rehabilitating Sand Dune Areas Planted With Acacia Spp","volume":"33","author":"Barnet","year":"1985","journal-title":"Aust. J. Bot."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/BF00014783","article-title":"Distribution and characteristics of root-nodule bacteria isolated from Australian Acacia spp","volume":"135","author":"Barnet","year":"1991","journal-title":"Plant Soil"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1128\/AEM.64.10.3989-3997.1998","article-title":"Molecular Diversity of Rhizobia Occurring on Native Shrubby Legumes in Southeastern Australia","volume":"64","author":"Lafay","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_52","first-page":"107","article-title":"Symbiotic diversity in the cosmopolitan genus Acacia","volume":"41","author":"Leary","year":"2006","journal-title":"Symbiosis"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1111\/j.1472-4642.2012.00920.x","article-title":"Mutualisms are not constraining cross?continental invasion success of Acacia species within Australia","volume":"18","author":"Birnbaum","year":"2012","journal-title":"Divers. Distrib."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Vel\u00e1zquez, E., Mart\u00ednez-Hidalgo, P., Carro, L., Alonso, P., Peix, A., Trujillo, M.E., and Mart\u00ednez-Molina, E. (2014). Chapter 10, Nodular endophytes: An untapped diversity. Beneficial Plant Microbial Interactions: Ecology and Applications, B\u00e9len Rodelas Gonzalez and J\u00e9sus Gonzalez-Lopez. [1st ed.].","DOI":"10.1201\/b15251-11"},{"key":"ref_55","first-page":"215","article-title":"Identification of Sinorhizobium (Ensifer) medicae based on a specific genomic sequence unveiled by M13-PCR fingerprinting","volume":"12","author":"Dourado","year":"2009","journal-title":"Int. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1007\/s00248-007-9243-0","article-title":"Genetic Diversity of Acacia seyal Del. Rhizobial Populations Indigenous to Senegalese Soils in Relation to Salinity and pH of the Sampling Sites","volume":"54","author":"Diouf","year":"2007","journal-title":"Microb. Ecol."},{"key":"ref_57","first-page":"269","article-title":"Nodulation in black locust by the \u03b3-proteobacteria Pseudomonas sp. and the \u03b2-proteobacteria Burkholderia sp. Systematic Appied","volume":"33","author":"Shiraishi","year":"2010","journal-title":"Microbiology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1007\/s11274-013-1278-4","article-title":"Characterization of root-nodule bacteria isolated from Vicia faba and selection of plant growth promoting isolates","volume":"29","author":"Chebil","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/BF02220715","article-title":"Competition between strains ofBradyrhizobium japonicum for nodulation of soybeans at different nitrogen fertilizer levels","volume":"117","author":"Brutti","year":"1989","journal-title":"Plant Soil"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1038\/nature01931","article-title":"Host sanctions and the legume\u2013rhizobium mutualism","volume":"425","author":"Kiers","year":"2003","journal-title":"Nature"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/pce.13348","article-title":"Legume nodulation: The host controls the party","volume":"42","author":"Ferguson","year":"2018","journal-title":"Plant Cell Environ."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.agron.2014.09.001","article-title":"Advances in Host Plant and Rhizobium Genomics to Enhance Symbiotic Nitrogen Fixation in Grain Legumes","volume":"129","author":"Dwivedi","year":"2015","journal-title":"Adv. Agron."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1111\/j.1461-0248.2005.00795.x","article-title":"Interpreting the \u201cselection effect\u201d of biodiversity on ecosystem function","volume":"8","author":"Fox","year":"2005","journal-title":"Ecol. Lett."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1046\/j.1472-4642.2000.00083.x","article-title":"Naturalization and invasion of alien plants: Concepts and definitions","volume":"6","author":"Richardson","year":"2000","journal-title":"Divers. Distrib."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s11104-008-9833-8","article-title":"Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants","volume":"321","author":"Franche","year":"2008","journal-title":"Plant Soil"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1139\/W09-056","article-title":"Plasmid transfer systems in the rhizobia","volume":"55","author":"Ding","year":"2009","journal-title":"Can. J. Microbiol."}],"container-title":["Diversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-2818\/12\/6\/250\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:40:54Z","timestamp":1760175654000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-2818\/12\/6\/250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,19]]},"references-count":66,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["d12060250"],"URL":"https:\/\/doi.org\/10.3390\/d12060250","relation":{},"ISSN":["1424-2818"],"issn-type":[{"value":"1424-2818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,19]]}}}