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We deployed both substrates in two different locations (R\u00eda de Vigo and R\u00eda de Ferrol, NW Iberian coast) and collected them after 6, 9, and 12 months to assess species composition of the colonizing communities through high-throughput sequencing of amplicons within the barcode region of the mitochondrial cytochrome c oxidase I (COI-5P) and the V4 domain of the 18S rRNA genes. We observed a consistently low similarity in species composition between substrate types, independently of sampling times and sites. A large fraction of exclusive species was recorded for a given substrate (up to 72%), whereas only up to 32% of species were recorded in both substrates. The shape and structural complexity of the substrate strongly affected the colonization preferences, with ASMS detecting more exclusive crustacean and gastropod species and a broader diversity of taxonomic groups (e.g., Entoprocta and Pycnogonida were detected exclusively in ASMS). We demonstrate that despite the customary use of ARMS for macrozoobenthos monitoring, by using ASMS we complemented the recovery of species and enlarged the scope of the taxonomic diversity recorded.<\/jats:p>","DOI":"10.3390\/d15050657","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T09:56:18Z","timestamp":1683885378000},"page":"657","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Artificial Seaweed Substrates Complement ARMS in DNA Metabarcoding-Based Monitoring of Temperate Coastal Macrozoobenthos"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4440-1298","authenticated-orcid":false,"given":"Barbara R.","family":"Leite","sequence":"first","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA) and ARNET-Aquatic Research Network, Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"UVIGO Marine Research Centre (CIM-UVIGO), ECIMAT Marine Station, Toralla Island, S\/N, 36331 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-0676","authenticated-orcid":false,"given":"Sofia","family":"Duarte","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA) and ARNET-Aquatic Research Network, Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Jes\u00fas S.","family":"Troncoso","sequence":"additional","affiliation":[{"name":"UVIGO Marine Research Centre (CIM-UVIGO), ECIMAT Marine Station, Toralla Island, S\/N, 36331 Vigo, Spain"},{"name":"Department of Ecology and Animal Biology, Marine Sciences Faculty, University of Vigo, Campus Lagoas-Marcosende, 36310 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5398-3942","authenticated-orcid":false,"given":"Filipe O.","family":"Costa","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA) and ARNET-Aquatic Research Network, Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1146\/annurev-marine-041911-111611","article-title":"Climate Change Impacts on Marine Ecosystems","volume":"4","author":"Doney","year":"2012","journal-title":"Annu. 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