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research","award":["CEECIND\/00095\/2017"],"award-info":[{"award-number":["CEECIND\/00095\/2017"]}]},{"name":"individual research","award":["CEECINST\/00051\/2018"],"award-info":[{"award-number":["CEECINST\/00051\/2018"]}]},{"name":"individual research","award":["2020.09563.BD"],"award-info":[{"award-number":["2020.09563.BD"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diversity"],"abstract":"<jats:p>The ever-growing demand for sea cucumbers is a threat to these echinoderms and their habitats; however, a way to relieve stock pressure lies in meeting demand through aquaculture. As such, this study aimed to improve the growth and survival percentage of Holothuria arguinensis during larval development, settlement and juvenile growth. Three diets of microalgae (Diet RbPt = Rhodomonas baltica + Phaeodactylum tricornutum; Diet RbSm = Rhodomonas baltica + Skeletonema marinoi; Diet RbCc = Rhodomonas baltica + Chaetoceros calcitrans) were administered daily to the larval phase. Additionally, three substrates were supplied (pvc rolls, wavy pvc plaques and tile) at three different depths in order to determine the most favourable for settlement. Lastly, the potential benefits on growth and survival from introducing macroalgae (Sacchoriza polyschides) in the juvenile diet were assessed over a period of 6 months. Despite larvae under diet RbPt having presented a larger mean width, H. arguinensis fed with Diet RbCc presented a higher settlement survival at the end of the trial. No preferences were noted among the tested substrates, regardless of diet. Lastly, juveniles with added S. polyschides in their diet showed increased growth in mean weight and length when compared to individuals that were not fed with this seaweed.<\/jats:p>","DOI":"10.3390\/d15060722","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T02:39:47Z","timestamp":1685587187000},"page":"722","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Optimizing Growth and Rearing Techniques for Larvae and Juveniles of the Sea Cucumber Holothuria arguinensis"],"prefix":"10.3390","volume":"15","author":[{"given":"Jo\u00e3o","family":"Sousa","sequence":"first","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, ESTM, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0466-3464","authenticated-orcid":false,"given":"Pedro M.","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Departamento de Biologia Animal, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6539-5830","authenticated-orcid":false,"given":"Ana C.","family":"Brito","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Eliana","family":"Ven\u00e2ncio","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, ESTM, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6500-2133","authenticated-orcid":false,"given":"Francisco","family":"Azevedo e Silva","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Tom\u00e1s","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Ana","family":"Amorim","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Maria Lu\u00edsa","family":"D\u00e2maso-Rodrigues","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1397-9206","authenticated-orcid":false,"given":"Ana","family":"Pombo","sequence":"additional","affiliation":[{"name":"MARE\u2013Marine and Environmental Sciences Centre\/ARNET, ESTM, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Yuan, X., McCoy, S.J., Du, Y., Widdicombe, S., and Hall-Spencer, J.M. 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