{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:01:34Z","timestamp":1760241694291,"version":"build-2065373602"},"reference-count":80,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T00:00:00Z","timestamp":1532649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Prostate cancer is the third highest cause of male mortality in the developed world, with the burden of the disease increasing dramatically with demographic change. There are significant limitations to the current diagnostic regimens and no established effective screening modality. To this end, research has discovered hundreds of potential \u2018biomarkers\u2019 that may one day be of use in screening, diagnosis or prognostication. However, the barriers to bringing biomarkers to clinical evaluation and eventually into clinical usage have yet to be realised. This is an operational challenge that requires some new thinking and development of paradigms to increase the efficiency of the laboratory process and add \u2018value\u2019 to the clinician. Value comes in various forms, whether it be a process that is seamlessly integrated into the hospital laboratory environment or one that can provide additional \u2018information\u2019 for the clinical pathologist in terms of risk profiling. We describe, herein, an efficient and tissue-conserving pipeline that uses Tissue Microarrays in a semi-automated process that could, one day, be integrated into the hospital laboratory domain, using seven putative prostate cancer biomarkers for illustration.<\/jats:p>","DOI":"10.3390\/diagnostics8030049","type":"journal-article","created":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T12:20:03Z","timestamp":1532694003000},"page":"49","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Quantitative Analysis of Seven New Prostate Cancer Biomarkers and the Potential Future of the \u2018Biomarker Laboratory\u2019"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9494-0946","authenticated-orcid":false,"given":"Kevin","family":"Cao","sequence":"first","affiliation":[{"name":"Prostate Cancer Research Centre at the Centre for Stem Cells and Regenerative Medicine, King\u2019s College London, London WC2R 2LS, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8508-3754","authenticated-orcid":false,"given":"Callum","family":"Arthurs","sequence":"additional","affiliation":[{"name":"Prostate Cancer Research Centre at the Centre for Stem Cells and Regenerative Medicine, King\u2019s College London, London WC2R 2LS, UK"}]},{"given":"Ali","family":"Atta-ul","sequence":"additional","affiliation":[{"name":"Prostate Cancer Research Centre, University College London, London WC1E 6BT, UK"}]},{"given":"Michael","family":"Millar","sequence":"additional","affiliation":[{"name":"Queen\u2019s Medical Research Institute, University of Edinburgh, Edinburgh EH8 9YL, UK"}]},{"given":"Mariana","family":"Beltran","sequence":"additional","affiliation":[{"name":"Aquila BioMedical, Nine, Edinburgh BioQuarter, 9 Little France Road, Edinburgh EH16 4UX, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2484-6994","authenticated-orcid":false,"given":"Jochen","family":"Neuhaus","sequence":"additional","affiliation":[{"name":"Head of Urology Research Laboratories, University of Leipzig, Department of Urology, Research Laboratory, Liebigstr. 19, Building C, 04103 Leipzig, Germany"}]},{"given":"Lars-Christian","family":"Horn","sequence":"additional","affiliation":[{"name":"Division of Gynecologic, Breast & Perinatal Pathology, University Hospital Leipzig, Liebigstasse 24 D, 04103 Leipzig, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3171-4666","authenticated-orcid":false,"given":"Rui","family":"Henrique","sequence":"additional","affiliation":[{"name":"Department of Pathology, Portuguese Oncology Institute of Porto, 4200-072 Porto, Portugal"},{"name":"Department of Pathology and Molecular Immunology, Abel Salazar Institute of Biomedical Sciences, University of Porto, 4099-002 Porto, Portugal"}]},{"given":"Aamir","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Prostate Cancer Research Centre at the Centre for Stem Cells and Regenerative Medicine, King\u2019s College London, London WC2R 2LS, UK"},{"name":"Prostate Cancer Research Centre, University College London, London WC1E 6BT, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9425-8624","authenticated-orcid":false,"given":"Christopher","family":"Thrasivoulou","sequence":"additional","affiliation":[{"name":"Research Department of Cell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Rockefeller Building, University College London, London WC1E 6BT, UK"}]}],"member":"1968","published-online":{"date-parts":[[2018,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3322\/caac.21262","article-title":"Global cancer statistics, 2012","volume":"65","author":"Torre","year":"2015","journal-title":"CA-Cancer J. Clin."},{"key":"ref_2","unstructured":"Statistics OFN (2018, January 15). Statistical Bulletin: Cancer Registration Statistics, England: 2015, Available online: https:\/\/www.ons.gov.uk\/peoplepopulationandcommunity\/healthandsocialcare\/conditionsanddiseases\/bulletins\/cancerregistrationstatisticsengland\/2015."},{"key":"ref_3","unstructured":"National Cancer Intelligence Network (2018, January 15). Five and Ten Year Cancer Prevalence 2006. Available online: www.ncin.org.uk\/view?rid=76."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1016\/S1470-2045(12)70211-5","article-title":"Global cancer transitions according to the Human Development Index (2008-2030): A population-based study","volume":"13","author":"Bray","year":"2012","journal-title":"Lancet Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1056\/NEJMoa031918","article-title":"Prevalence of prostate cancer among men with a prostate-specific antigen level < or =4.0 ng per milliliter","volume":"350","author":"Thompson","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1001\/jama.294.1.66","article-title":"Operating characteristics of prostate-specific antigen in men with an initial PSA level of 3.0 ng\/mL or lower","volume":"294","author":"Thompson","year":"2005","journal-title":"JAMA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.canlet.2008.04.047","article-title":"Molecular alterations in prostate cancer","volume":"271","author":"Reynolds","year":"2008","journal-title":"Cancer Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.1464-410X.2007.07138.x","article-title":"The discovery of prostate-specific antigen","volume":"101","author":"Rao","year":"2008","journal-title":"BJU Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1038\/nrc2351","article-title":"Prostate-specific antigen and prostate cancer: Prediction, detection and monitoring","volume":"8","author":"Lilja","year":"2008","journal-title":"Nat. Rev. Cancer"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1002\/pros.23166","article-title":"Clinical significance of prospectively assigned Gleason tertiary pattern 4 in contemporary Gleason score 3+3=6 prostate cancer","volume":"76","author":"Doshi","year":"2016","journal-title":"Prostate"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.eururo.2015.06.046","article-title":"A Contemporary Prostate Cancer Grading System: A Validated Alternative to the Gleason Score","volume":"69","author":"Epstein","year":"2016","journal-title":"Eur. Urol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"426","DOI":"10.5858\/arpa.2011-0495-RA","article-title":"Implications of the International Society of Urological Pathology modified Gleason grading system","volume":"136","author":"Egevad","year":"2012","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1097\/ALN.0b013e3181d47604","article-title":"Statistical evaluation of a biomarker","volume":"112","author":"Ray","year":"2010","journal-title":"Anesthesiology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1038\/nbt1235","article-title":"Protein biomarker discovery and validation: The long and uncertain path to clinical utility","volume":"24","author":"Rifai","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1002\/pmic.201000460","article-title":"Proteomic technologies for the identification of disease biomarkers in serum: Advances and challenges ahead","volume":"11","author":"Ray","year":"2011","journal-title":"Proteomics"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Tonry, C.L., Leacy, E., Raso, C., Finn, S.P., Armstrong, J., and Pennington, S.R. (2016). The Role of Proteomics in Biomarker Development for Improved Patient Diagnosis and Clinical Decision Making in Prostate Cancer. Diagnostics (Basel), 6.","DOI":"10.3390\/diagnostics6030027"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","article-title":"High resolution two-dimensional electrophoresis of proteins","volume":"250","year":"1975","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3109\/10408363.2010.503558","article-title":"Application of proteomic technologies for prostate cancer detection, prognosis, and tailored therapy","volume":"47","author":"Fredolini","year":"2010","journal-title":"Crit. Rev. Clin. Lab. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s13148-016-0303-5","article-title":"UroMark-a urinary biomarker assay for the detection of bladder cancer","volume":"9","author":"Feber","year":"2017","journal-title":"Clin. Epigenetics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.exger.2005.06.011","article-title":"Tissue microarray technology: Principles, pitfalls and perspectives--lessons learned from hematological malignancies","volume":"40","author":"Tzankov","year":"2005","journal-title":"Exp. Gerontol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.tranon.2017.06.002","article-title":"Systematic Analysis of the Expression of the Mitochondrial ATP Synthase (Complex V) Subunits in Clear Cell Renal Cell Carcinoma","volume":"10","author":"Bruggemann","year":"2017","journal-title":"Transl. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.cellsig.2017.09.006","article-title":"Diindolylmethane and its halogenated derivatives induce protective autophagy in human prostate cancer cells via induction of the oncogenic protein AEG-1 and activation of AMP-activated protein kinase (AMPK)","volume":"40","author":"Draz","year":"2017","journal-title":"Cell Signal."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1002\/pros.23106","article-title":"GSK-3beta controls autophagy by modulating LKB1-AMPK pathway in prostate cancer cells","volume":"76","author":"Sun","year":"2016","journal-title":"Prostate"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.3892\/ijo.2017.3878","article-title":"Ectopic expression of the ATP synthase beta subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis","volume":"50","author":"Li","year":"2017","journal-title":"Int. J. Oncol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"19938","DOI":"10.1074\/jbc.273.32.19938","article-title":"Identification of diazepam-binding Inhibitor\/Acyl-CoA-binding protein as a sterol regulatory element-binding protein-responsive gene","volume":"273","author":"Swinnen","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1158\/1940-6207.CAPR-14-0057","article-title":"Acyl-coenzyme A-binding protein regulates Beta-oxidation required for growth and survival of non-small cell lung cancer","volume":"7","author":"Harris","year":"2014","journal-title":"Cancer Prev. Res. (Phila)"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.4049\/jimmunol.169.2.1102","article-title":"Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies","volume":"169","author":"Wang","year":"2002","journal-title":"J. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.2353\/ajpath.2009.090155","article-title":"Genoproteomic mining of urothelial cancer suggests {gamma}-glutamyl hydrolase and diazepam-binding inhibitor as putative urinary markers of outcome after chemotherapy","volume":"175","author":"Pollard","year":"2009","journal-title":"Am. J. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0303-7207(94)90118-X","article-title":"Androgen regulation of the messenger RNA encoding diazepam-binding inhibitor\/acyl-CoA-binding protein in the human prostatic adenocarcinoma cell line LNCaP","volume":"104","author":"Swinnen","year":"1994","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1089\/dna.1996.15.197","article-title":"A human gene encoding diazepam-binding inhibitor\/acy1-CoA-binding protein: Transcription and hormonal regulation in the androgen-sensitive human prostatic adenocarcinoma cell line LNCaP","volume":"15","author":"Swinnen","year":"1996","journal-title":"DNA Cell Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1126\/science.272.5267.1497","article-title":"Principles of chaperone-assisted protein folding: Differences between in vitro and in vivo mechanisms","volume":"272","author":"Frydman","year":"1996","journal-title":"Science"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1002\/ijc.2910610222","article-title":"A stress-inducible 72-kDa heat-shock protein (HSP72) is expressed on the surface of human tumor cells, but not on normal cells","volume":"61","author":"Multhoff","year":"1995","journal-title":"Int. J. Cancer"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1002\/elps.1150180341","article-title":"Analysis of polypeptide expression in benign and malignant human breast lesions","volume":"18","author":"Franzen","year":"1997","journal-title":"Electrophoresis"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1365-2141.1995.tb03395.x","article-title":"Analysis of heat-shock protein expression in myeloid leukaemia cells by flow cytometry","volume":"90","author":"Chant","year":"1995","journal-title":"Br. J. Haematol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"144","DOI":"10.3389\/fonc.2016.00144","article-title":"Hypoxia on the Expression of Hepatoma Upregulated Protein in Prostate Cancer Cells","volume":"6","author":"Espinoza","year":"2016","journal-title":"Front. Oncol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1021\/pr100921j","article-title":"Proteomics profiling of microdissected low- and high-grade prostate tumors identifies Lamin A as a discriminatory biomarker","volume":"10","author":"Skvortsov","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8988","DOI":"10.1158\/0008-5472.CAN-10-2225","article-title":"Heat shock protein 60 regulation of the mitochondrial permeability transition pore in tumor cells","volume":"70","author":"Ghosh","year":"2010","journal-title":"Cancer Res."},{"key":"ref_38","first-page":"7099","article-title":"Heat shock protein expression independently predicts clinical outcome in prostate cancer","volume":"60","author":"Cornford","year":"2000","journal-title":"Cancer Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1017.e1","DOI":"10.1016\/j.urology.2010.05.045","article-title":"Immunohistochemical expression of Hsp60 correlates with tumor progression and hormone resistance in prostate cancer","volume":"76","author":"Castilla","year":"2010","journal-title":"Urology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2850","DOI":"10.1158\/1078-0432.CCR-12-3084","article-title":"Translation initiation factor eIF3b expression in human cancer and its role in tumor growth and lung colonization","volume":"19","author":"Wang","year":"2013","journal-title":"Clin. Cancer Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/bjc.2013.688","article-title":"Role of EIF5A2, a downstream target of Akt, in promoting melanoma cell invasion","volume":"110","author":"Khosravi","year":"2014","journal-title":"Br. J. Cancer"},{"key":"ref_42","first-page":"579","article-title":"Anticancer potential of novel curcumin analogs towards castrate-resistant prostate cancer","volume":"52","author":"Chen","year":"2018","journal-title":"Int. J. Oncol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1097\/CCO.0000000000000367","article-title":"Targeting of protein translation as a new treatment paradigm for prostate cancer","volume":"29","author":"Ramamurthy","year":"2017","journal-title":"Curr. Opin. Oncol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"24047","DOI":"10.1074\/jbc.M800956200","article-title":"An oncogenic role for the phosphorylated h-subunit of human translation initiation factor eIF3","volume":"283","author":"Zhang","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1038\/sj.bjc.6601648","article-title":"Expression and copy number analysis of TRPS1, EIF3S3 and MYC genes in breast and prostate cancer","volume":"90","author":"Savinainen","year":"2004","journal-title":"Br. J. Cancer"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0090-4295(03)00776-3","article-title":"The molecular genetics of prostate cancer","volume":"62","author":"Visakorpi","year":"2003","journal-title":"Urology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1002\/(SICI)1098-2264(200006)28:2<203::AID-GCC9>3.0.CO;2-X","article-title":"Mapping the amplification of EIF3S3 in breast and prostate cancer","volume":"28","author":"Nupponen","year":"2000","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1590\/S0100-879X2010007500098","article-title":"Regulation of protein synthesis and the role of eIF3 in cancer","volume":"43","author":"Hershey","year":"2010","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.bbagrm.2014.10.005","article-title":"The role of eIF3 and its individual subunits in cancer","volume":"1849","author":"Hershey","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.1016\/S0002-9440(10)63060-X","article-title":"Amplification of EIF3S3 gene is associated with advanced stage in prostate cancer","volume":"159","author":"Saramaki","year":"2001","journal-title":"Am. J. Pathol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1016\/S0002-9440(10)65433-8","article-title":"Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer","volume":"154","author":"Nupponen","year":"1999","journal-title":"Am. J. Pathol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1007\/s00018-014-1769-y","article-title":"BRI2 ectodomain affects Abeta42 fibrillation and tau truncation in human neuroblastoma cells","volume":"72","author":"Oliveira","year":"2015","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.bbadis.2014.12.018","article-title":"The ITM2B (BRI2) gene is a target of BCL6 repression: Implications for lymphomas and neurodegenerative diseases","volume":"1852","author":"Baron","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_54","first-page":"97","article-title":"Atypical spindle cell lipoma: A clinicopathologic, immunohistochemical, and molecular study emphasizing its relationship to classical spindle cell lipoma","volume":"465","author":"Creytens","year":"2014","journal-title":"Virchows Arch."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1095\/biolreprod.115.130252","article-title":"Reduced Fertility and Altered Epididymal and Sperm Integrity in Mice Lacking ADAM7","volume":"93","author":"Choi","year":"2015","journal-title":"Biol. Reprod."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/pros.10272","article-title":"Extensive analysis of the 13q14 region in human prostate tumors: DNA analysis and quantitative expression of genes lying in the interval of deletion","volume":"57","author":"Latil","year":"2003","journal-title":"Prostate"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ruppender, N., Larson, S., Lakely, B., Kollath, L., Brown, L., Coleman, I., Coleman, R., Nguyen, H., Nelson, P.S., and Corey, E. (2015). Cellular Adhesion Promotes Prostate Cancer Cells Escape from Dormancy. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0130565"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/sj.pcan.4500663","article-title":"Dependence of metastatic cancer cell invasion on MLCK-catalyzed phosphorylation of myosin regulatory light chain","volume":"6","author":"Tohtong","year":"2003","journal-title":"Prostate Cancer Prostatic Dis."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1002\/path.4485","article-title":"AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival","volume":"235","author":"Gronau","year":"2015","journal-title":"J. Pathol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1080\/15476286.2015.1137421","article-title":"Studies on human eRF3-PABP interaction reveal the influence of eRF3a N-terminal glycin repeat on eRF3-PABP binding affinity and the lower affinity of eRF3a 12-GGC allele involved in cancer susceptibility","volume":"13","author":"Jerbi","year":"2016","journal-title":"RNA Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.canlet.2015.07.010","article-title":"PABPC1 interacts with AGO2 and is responsible for the microRNA mediated gene silencing in high grade hepatocellular carcinoma","volume":"367","author":"Zhang","year":"2015","journal-title":"Cancer Lett."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1002\/pmic.201300477","article-title":"Exosome-mediated extracellular release of polyadenylate-binding protein 1 in human metastatic duodenal cancer cells","volume":"14","author":"Ohshima","year":"2014","journal-title":"Proteomics"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Eisermann, K., Dar, J.A., Dong, J., Wang, D., Masoodi, K.Z., and Wang, Z. (2015). Poly (A) Binding Protein Cytoplasmic 1 Is a Novel Co-Regulator of the Androgen Receptor. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0128495"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Symes, A.J., Eilertsen, M., Millar, M., Nariculam, J., Freeman, A., Notara, M., Feneley, M.R., Patel, H.R., Masters, J.R., and Ahmed, A. (2013). Quantitative analysis of BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer tissue. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0084295"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Arthurs, C., Murtaza, B.N., Thomson, C., Dickens, K., Henrique, R., Patel, H.R.H., Beltran, M., Millar, M., Thrasivoulou, C., and Ahmed, A. (2017). Expression of ribosomal proteins in normal and cancerous human prostate tissue. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0186047"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/aja.2008.22","article-title":"Utility of tissue microarrays for profiling prognostic biomarkers in clinically localized prostate cancer: The expression of BCL-2, E-cadherin, Ki-67 and p53 as predictors of biochemical failure after radical prostatectomy with nested control for clinical and pathological risk factors","volume":"11","author":"Nariculam","year":"2009","journal-title":"Asian J. Androl."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Wang, Q., Symes, A.J., Kane, C.A., Freeman, A., Nariculam, J., Munson, P., Thrasivoulou, C., Masters, J.R., and Ahmed, A. (2010). A novel role for Wnt\/Ca2+ signaling in actin cytoskeleton remodeling and cell motility in prostate cancer. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010456"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1038\/nm0798-844","article-title":"Tissue microarrays for high-throughput molecular profiling of tumor specimens","volume":"4","author":"Kononen","year":"1998","journal-title":"Nat. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1016\/S0002-9440(10)63075-1","article-title":"Tissue microarrays for rapid linking of molecular changes to clinical endpoints","volume":"159","author":"Torhorst","year":"2001","journal-title":"Am. J. Pathol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1016\/j.juro.2007.08.055","article-title":"Biomarkers for prostate cancer detection","volume":"178","author":"Parekh","year":"2007","journal-title":"J. Urol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.clinbiochem.2012.12.012","article-title":"The role of proteomics in prostate cancer research: Biomarker discovery and validation","volume":"46","author":"Pin","year":"2013","journal-title":"Clin. Biochem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1074\/mcp.M115.047928","article-title":"Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation","volume":"14","author":"Shah","year":"2015","journal-title":"Mol. Cell. Proteom."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1038\/nature07762","article-title":"Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression","volume":"457","author":"Sreekumar","year":"2009","journal-title":"Nature"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1016\/j.jprot.2011.11.002","article-title":"Identification and quantification of peptides and proteins secreted from prostate epithelial cells by unbiased liquid chromatography tandem mass spectrometry using goodness of fit and analysis of variance","volume":"75","author":"Florentinus","year":"2012","journal-title":"J. Proteom."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1373\/clinchem.2006.077370","article-title":"Discovery of candidate tumor markers for prostate cancer via proteomic analysis of cell culture-conditioned medium","volume":"53","author":"Sardana","year":"2007","journal-title":"Clin. Chem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1074\/mcp.M112.023887","article-title":"Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression","volume":"12","author":"Saraon","year":"2013","journal-title":"Mol. Cell. Proteom."},{"key":"ref_77","first-page":"3058","article-title":"Castration-induced up-regulation of insulin-like growth factor binding protein-5 potentiates insulin-like growth factor-I activity and accelerates progression to androgen independence in prostate cancer models","volume":"60","author":"Miyake","year":"2000","journal-title":"Cancer Res."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Brooks, J.D., Wei, W., Hawley, S., Auman, H., Newcomb, L., Boyer, H., Fazli, L., Simko, J., Hurtado-Coll, A., and Troyer, D.A. (2015). Evaluation of ERG and SPINK1 by Immunohistochemical Staining and Clinicopathological Outcomes in a Multi-Institutional Radical Prostatectomy Cohort of 1067 Patients. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0132343"},{"key":"ref_79","unstructured":"Champy, J., and Hammer, M. (1995). Reengineering the Corporation: A Manifesto for Business Revolution, Nichlas Brealey Publishing Co."},{"key":"ref_80","unstructured":"Chase, R.B., Jacobs, F.R., and Aquilano, N.J. (2005). Operations Management for Competitive Advantage, Publishing Co.. [11th ed.]. McGraw-Hill Higher Education."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/8\/3\/49\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:14:49Z","timestamp":1760195689000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/8\/3\/49"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,27]]},"references-count":80,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,9]]}},"alternative-id":["diagnostics8030049"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics8030049","relation":{},"ISSN":["2075-4418"],"issn-type":[{"type":"electronic","value":"2075-4418"}],"subject":[],"published":{"date-parts":[[2018,7,27]]}}}