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Larval stages of fish are highly sensitive to environmental change and anthropogenic pressures, and Marine Protected Areas (MPA) can help mitigate the impacts on fish populations. This study investigated the environmental drivers of the temporal and spatial patterns of the larval fish assemblages inhabiting a small coastal MPA along the NW Portuguese Iberian Coast. Seasonal surveys were conducted over two years at nine sampling stations distributed throughout the MPA to collect larval fish samples and water parameters. Results showed that a total of 39 different fish taxa were identified. In terms of abundance, reef-associated species, such as Parablennius gattorugine (54.6%), and marine species that use estuaries as nursery areas, such as Ammodytes tobianus (15.7%) and Clupeidae n.i. (8.8%) dominated the larval fish assemblages. The larval fish assemblages were characterized by a strong temporal pattern that, according to CCA analyses, was related to the temporal variability of water temperature, pH, chlorophyll \u03b1, TPM, and also the river flow of an adjacent river. This study showed that 47% of the fish larvae belonged to commercially exploited species, highlighting the importance of this MPA as a nursery area for the early life stages of the fish population. Overall, these new findings emphasize the role of MPAs in ensuring the connectivity of fish species between marine-estuarine habitats and enhancing the conservation of fish populations.<\/jats:p>","DOI":"10.3390\/ecologies4020019","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T10:11:25Z","timestamp":1682071885000},"page":"288-309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Assessing Environmental Control on Temporal and Spatial Patterns of Larval Fish Assemblages in a Marine Protected Area"],"prefix":"10.3390","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8276-1011","authenticated-orcid":false,"given":"R\u00faben","family":"Pereira","sequence":"first","affiliation":[{"name":"CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS, Abel Salazar Institute of Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7028-2168","authenticated-orcid":false,"given":"Sabrina M.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS, Abel Salazar Institute of Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"given":"Diogo M.","family":"Silva","sequence":"additional","affiliation":[{"name":"CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS, Abel Salazar Institute of Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"given":"Sandra","family":"Ramos","sequence":"additional","affiliation":[{"name":"CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.marpol.2012.02.021","article-title":"The science of European marine reserves: Status, efficacy, and future needs","volume":"36","author":"Fenberg","year":"2012","journal-title":"Mar. Policy"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1111\/j.1461-0248.2008.01166.x","article-title":"Marine reserves: Size and age do matter","volume":"11","author":"Claudet","year":"2008","journal-title":"Ecol. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1890\/1051-0761(2003)013[0117:TIOMRD]2.0.CO;2","article-title":"The impact of marine reserves: Do reserves work and does reserve size matter?","volume":"13","author":"Halpern","year":"2003","journal-title":"Ecol. Appl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ocecoaman.2016.07.005","article-title":"An overview of Marine Protected Areas in SW Europe: Factors contributing to their management effectiveness","volume":"132","author":"Batista","year":"2016","journal-title":"Ocean Coast. Manag."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.marpol.2016.06.021","article-title":"A regulation-based classification system for Marine Protected Areas (MPAs)","volume":"72","author":"Claudet","year":"2016","journal-title":"Mar. Policy"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1890\/08-2131.1","article-title":"Marine reserves: Fish life history and ecological traits matter","volume":"20","author":"Claudet","year":"2010","journal-title":"Ecol. Appl."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Pipitone, C., Agnetta, D., Zenone, A., Giacalone, V.M., Badalamenti, F., Fiorentino, F., Rinelli, P., Sinopoli, M., Fern\u00e1ndez, T.V., and D\u2019Anna, G. (2023). When the Trawl Ban Is a Good Option: Opportunities to Restore Fish Biomass and Size Structure in a Mediterranean Fisheries Restricted Area. Sustainability, 15.","DOI":"10.3390\/su15032425"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1017\/S0376892900000175","article-title":"Effects of marine protected areas on recruitment processes with special reference to Mediterranean littoral ecosystems","volume":"27","author":"Planes","year":"2000","journal-title":"Environ. Conserv."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/ddi.12250","article-title":"Additive effects of climate change on connectivity between marine protected areas and larval supply to fished areas","volume":"21","author":"Andrello","year":"2015","journal-title":"Divers. Distrib."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jnc.2016.04.004","article-title":"Spillover from marine protected areas to adjacent fisheries has an ecological and a fishery component","volume":"32","author":"Claudet","year":"2016","journal-title":"J. Nat. Conserv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1038\/nature13022","article-title":"Global conservation outcomes depend on marine protected areas with five key features","volume":"506","author":"Edgar","year":"2014","journal-title":"Nature"},{"key":"ref_12","unstructured":"IUCN (2020, October 01). Marine Protected Areas and Climate Change, Issues Brief. Available online: https:\/\/www.iucn.org\/sites\/dev\/files\/mpas_and_climate_change_issues_brief.pdf."},{"key":"ref_13","unstructured":"EEA (2015). Marine Protected Areas in Europes Seas. An Overview and Perspectives for the Future, European Environmental Agengy Report; European Environmental Agency."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.ecss.2006.08.020","article-title":"Horizontal spatial and temporal distribution patterns of nearshore larval fish assemblages at a temperate rocky shore","volume":"71","author":"Borges","year":"2007","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_15","first-page":"376","article-title":"Short-term Temporal Fluctuation of Very-nearshore Larval Fish Assemblages at the Arr\u00e1bida Marine Park (Portugal)","volume":"56","author":"Borges","year":"2009","journal-title":"J. Coast. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.seares.2013.02.004","article-title":"Seasonal variability of rocky reef fish assemblages: Detecting functional and structural changes due to fishing effects","volume":"79","author":"Henriques","year":"2013","journal-title":"J. Sea Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.ocecoaman.2016.11.017","article-title":"Changes in fish assemblage structure after implementation of Marine Protected Areas in the south western coast of Portugal","volume":"135","author":"Pereira","year":"2017","journal-title":"Ocean Coast. Manag."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/fog.12269","article-title":"Small scale temporal patterns of recruitment and hatching of Atlantic horse mackerel (L.) at a nearshore reef area","volume":"27","author":"Klein","year":"2018","journal-title":"Fish. Oceanogr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev.marine.010908.163917","article-title":"Marine primary production in relation to climate variability and change","volume":"3","author":"Chavez","year":"2011","journal-title":"Annu. Rev. Mar. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.ecss.2011.11.029","article-title":"Drivers of larval fish assemblage shift during the spring-summer transition in the coastal Mediterranean","volume":"97","author":"Jordi","year":"2012","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.csr.2018.03.009","article-title":"Zooplankton seasonality across a latitudinal gradient in the Northeast Atlantic Shelves Province","volume":"160","author":"Fanjul","year":"2018","journal-title":"Cont. Shelf Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.seares.2015.06.009","article-title":"Seasonality of the plankton community at an east and west coast monitoring site in Scottish waters","volume":"105","author":"Bresnan","year":"2015","journal-title":"J. Sea Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1006\/ecss.1996.0067","article-title":"Ichthyoplankton Assemblage Structure in Mississippi Sound as Revealed by Canonical Correspondence Analysis","volume":"43","author":"Rakocinski","year":"1996","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1093\/plankt\/fbi104","article-title":"Environmental forcing and larval fish assemblage dynamics in the Lima River estuary (northwest Portugal)","volume":"28","author":"Ramos","year":"2006","journal-title":"J. Plankton Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0065-2881(08)60202-3","article-title":"Plankton Production and Year-class Strength in Fish Populations: An Update of the Match\/Mismatch Hypothesis","volume":"Volume 26","author":"Blaxter","year":"1990","journal-title":"Advances in Marine Biology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3354\/meps120011","article-title":"The match\/mismatch hypothesis and the feeding success of fish larvae in ice-covered southeastern Hudson Bay","volume":"120","author":"Fortier","year":"1995","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kristiansen, T., Drinkwater, K.F., Lough, R.G., and Sundby, S. (2011). Recruitment variability in North Atlantic cod and match-mismatch dynamics. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017456"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev.marine.010908.163757","article-title":"Larval dispersal and marine population connectivity","volume":"1","author":"Cowen","year":"2009","journal-title":"Annu. Rev. Mar. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3354\/meps08176","article-title":"Variability in transport of fish eggs and larvae. IV. Interannual variability in larval stage duration of immigrating plaice in the Dutch Wadden Sea","volume":"390","author":"Bolle","year":"2009","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_30","unstructured":"Wolanski, E., and McLusky, D. (2011). Treatise on Estuarine and Coastal Science, Academic Press."},{"key":"ref_31","first-page":"1462","article-title":"Larval fish dispersal along an estuarine\u2013ocean gradient","volume":"74","author":"Ramos","year":"2017","journal-title":"Dispersal Dur. Early Life Hist. Fish"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/S0169-5347(03)00189-7","article-title":"Benefits beyond boundaries: The fishery effects of marine reserves","volume":"18","author":"Gell","year":"2003","journal-title":"Trends Ecol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"248","DOI":"10.12681\/mms.14993","article-title":"Nest-mediated parental care in a marine fish: Are large-scale nesting habitats selected and do these habitats respond to small-scale requirements?","volume":"19","author":"Sinopoli","year":"2018","journal-title":"Mediterr. Mar. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.tree.2005.03.015","article-title":"The importance in fishery management of leaving the big ones","volume":"20","author":"Birkeland","year":"2005","journal-title":"Trends Ecol. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3354\/meps12642","article-title":"Spawning stock, egg production and larval survival in relation to small pelagic fish recruitment","volume":"617\u2013618","author":"Somarakis","year":"2019","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1111\/maec.12187","article-title":"Nest building in a Mediterranean wrasse (Symphodus ocellatus): Are the algae used randomly chosen or actively selected?","volume":"36","author":"Sinopoli","year":"2015","journal-title":"Mar. Ecol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s10750-015-2348-4","article-title":"Dealing with temporal variation and different life stages of whitemouth croaker Micropogonias furnieri (Actinopterygii, Sciaenidae) in species distribution modeling to improve essential estuarine fish habitat identification","volume":"762","author":"Costa","year":"2015","journal-title":"Hydrobiologia"},{"key":"ref_38","unstructured":"Pereira, R. (2020). Environmental Control of Planktonic Communities in a Coastal Marine Protected Area (PNLN). [Master\u2019s thesis, Universidade do Porto]."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.pocean.2007.04.021","article-title":"Physical oceanography of the western Iberia ecosystem: Latest views and challenges","volume":"74","author":"Relvas","year":"2007","journal-title":"Prog. Oceanogr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"C07020","DOI":"10.1029\/2008JC004744","article-title":"Spatiotemporal evolution of upwelling regime along the western coast of the Iberian Peninsula","volume":"113","author":"Alvarez","year":"2008","journal-title":"J. Geophys. Res. Ocean."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.dsr.2004.11.005","article-title":"Winter upper ocean circulation in the Western Iberian Basin\u2014Fronts, Eddies and Poleward Flows: An overview","volume":"52","author":"Peliz","year":"2005","journal-title":"Deep Sea Res. Part I Oceanogr. Res. Pap."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.seares.2015.07.005","article-title":"Immigration and early life stages recruitment of the European flounder (Platichthys flesus) to an estuarine nursery: The influence of environmental factors","volume":"107","author":"Amorim","year":"2016","journal-title":"J. Sea Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.seares.2010.01.010","article-title":"Recruitment of flatfish species to an estuarine nursery habitat (Lima estuary, NW Iberian Peninsula)","volume":"64","author":"Ramos","year":"2010","journal-title":"J. Sea Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.ecolind.2011.08.024","article-title":"Early life stages of fishes as indicators of estuarine ecosystem health","volume":"19","author":"Ramos","year":"2012","journal-title":"Ecol. Indic."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.ecss.2006.05.023","article-title":"Larval fish distribution in shallow coastal waters off North Western Iberia (NE Atlantic)","volume":"69","author":"Azeiteiro","year":"2006","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3989\/scimar.04081.08A","article-title":"Environmental factors affecting larval fish community in the salt marsh area of Guadiana estuary (Algarve, Portugal)","volume":"79","author":"Correia","year":"2015","journal-title":"Sci. Mar."},{"key":"ref_47","unstructured":"Russell, F.S. (1976). The Eggs and Planktonic Stages of British Marine Fishes, Academic Press."},{"key":"ref_48","unstructured":"R\u00e9, P., and Meneses, I. (2008). Early Stages of Marine Fishes Occurring in the Iberian Peninsula, IPIMAR."},{"key":"ref_49","unstructured":"Garc\u00eda, A., Rodriguez, J., and Alemany, F. (2018). A Guide to the Eggs and Larvae of 100 Common Western Mediterranean Sea Bony Fish Species, FAO."},{"key":"ref_50","unstructured":"Munk, P., and Nielsen, J.G. (2005). Eggs and Larvae of North Sea Fishes, Biofolia."},{"key":"ref_51","unstructured":"Parsons, T.R., Maita, Y., and Lalli, C.M. (1984). A Manual of Chemical and Biological Methods for Seawater Analysis, Pergamon."},{"key":"ref_52","unstructured":"(1966). Monographs on Oceanographic Methodology, UNESCO."},{"key":"ref_53","unstructured":"Grasshoff, K., Ehrhardt, M., and Kremling, K. (1983). Methods of Seawater Analysis, Verlag Chemie. Volume Second revised and extended edition."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/0043-1354(84)90215-X","article-title":"Nitrate reduction by shaking with cadmium: Alternative to cadmium columns","volume":"18","author":"Jones","year":"1984","journal-title":"Water Res."},{"key":"ref_55","unstructured":"APHA (1992). Standard Methods for the Examination of Water and Wastewater, American Public Health Association."},{"key":"ref_56","unstructured":"Shannon, C.E., and Weaver, W. (1963). The Mathematical Theory of Communications, University of Illinois Press."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0022-5193(66)90013-0","article-title":"The measurement of diversity in different types of biological collections","volume":"13","author":"Pielou","year":"1966","journal-title":"J. Theor. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"219","DOI":"10.3354\/meps07203","article-title":"Life strategies of fishes in European estuaries: The functional guild approach","volume":"354","author":"Franco","year":"2008","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_59","unstructured":"DGRM (2020). Recursos da Pesca, Dire\u00e7\u00e3o-Geral de Recursos Naturais, Seguran\u00e7a e Servi\u00e7os Mar\u00edtimos."},{"key":"ref_60","first-page":"975","article-title":"An Extension of the T-Method of Multiple Comparison to Include the Cases with Unequal Sample Sizes","volume":"68","author":"Stoline","year":"1973","journal-title":"J. Am. Stat. Assoc."},{"key":"ref_61","unstructured":"Clarke, K., Warwick, R., Clarke, K.R., and Warwick, R.M. (2001). Change in Marine Communities: An Approach to Statistical Analysis and Interpretation, Primer-E, Ltd."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/j.1442-9993.1993.tb00438.x","article-title":"Non-parametric multivariate analyses of changes in community structure","volume":"18","author":"Clarke","year":"1993","journal-title":"Aust. J. Ecol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"325","DOI":"10.2307\/1942268","article-title":"An Ordination of the Upland Forest Communities of Southern Wisconsin","volume":"27","author":"Bray","year":"1957","journal-title":"Ecol. Monogr."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.2307\/1938672","article-title":"Canonical Correspondence Analysis: A New Eigenvector Technique for Multivariate Direct Gradient Analysis","volume":"67","author":"Braak","year":"1986","journal-title":"Ecology"},{"key":"ref_65","unstructured":"Braak, C.J.F.t., and Smilauer, P. (2002). CANOCO Reference Manual and CanoDraw for Windows User\u2019s Guide: Software for Canonical Community Ordination (Version 4.5), Microcomputer Power."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.csr.2008.11.020","article-title":"The ichthyoplankton assemblage and the environmental variables off the NW and N Iberian Peninsula coasts, in early spring","volume":"29","author":"Rodriguez","year":"2009","journal-title":"Cont. Shelf Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.scitotenv.2018.12.273","article-title":"Microplastic contamination in an urban estuary: Abundance and distribution of microplastics and fish larvae in the Douro estuary","volume":"659","author":"Rodrigues","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.pocean.2007.04.018","article-title":"Sardine spawning off the European Atlantic coast: Characterization of and spatio-temporal variability in spawning habitat","volume":"74","author":"Bernal","year":"2007","journal-title":"Prog. Oceanogr."},{"key":"ref_69","first-page":"31","article-title":"Sardine spawning off Portugal","volume":"15","author":"Silva","year":"1990","journal-title":"Bol. Do Inst. Nac. De Investig. Das Pescas"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.pocean.2018.02.011","article-title":"Sardine (Sardina pilchardus) larval dispersal in the Iberian upwelling system, using coupled biophysical techniques","volume":"162","author":"Santos","year":"2018","journal-title":"Prog. Oceanogr."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"449","DOI":"10.3989\/scimar.2009.73n3449","article-title":"New insights into the early life ecology of Sardina pilchardus (Walbaum, 1792) in the northern Iberian Atlantic","volume":"73","author":"Ramos","year":"2009","journal-title":"Sci. Mar."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1093\/plankt\/fbl035","article-title":"Depth distribution of nearshore temperate fish larval assemblages near rocky substrates","volume":"28","author":"Beldade","year":"2006","journal-title":"J. Plankton Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.ecss.2009.07.018","article-title":"Spatial distribution and vertical migrations of fish larvae communities off Northwestern Iberia sampled with LHPR and Bongo nets","volume":"84","author":"Garrido","year":"2009","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.csr.2003.10.004","article-title":"Impact of a winter upwelling event on the distribution and transport of sardine (Sardina pilchardus) eggs and larvae off western Iberia: A retention mechanism","volume":"24","author":"Santos","year":"2004","journal-title":"Cont. Shelf Res."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"8636","DOI":"10.1038\/s41598-019-44918-5","article-title":"Assemblage structure of ichthyoplankton in the NE Atlantic in spring under contrasting hydrographic conditions","volume":"9","author":"Rodriguez","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.ecss.2006.05.032","article-title":"Ichthyoplankton dynamics in the Guadiana estuary and adjacent coastal area, South-East Portugal","volume":"70","author":"Faria","year":"2006","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jmarsys.2011.12.008","article-title":"Vertical patterns of ichthyoplankton at the interface between a temperate estuary and adjacent coastal waters: Seasonal relation to diel and tidal cycles","volume":"95","author":"Primo","year":"2012","journal-title":"J. Mar. Syst."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1093\/plankt\/fbi137","article-title":"Vertical distribution of the European sardine (Sardina pilchardus) larvae and its implications for their survival","volume":"28","author":"Santos","year":"2006","journal-title":"J. Plankton Res."},{"key":"ref_79","unstructured":"ICES (2019). Report of the ICES Advisory Committee, ICES."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1007\/s00227-006-0574-z","article-title":"Vertical structure of very nearshore larval fish assemblages in a temperate rocky coast","volume":"151","author":"Borges","year":"2006","journal-title":"Mar. Biol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"17065","DOI":"10.1038\/srep17065","article-title":"Born small, die young: Intrinsic, size-selective mortality in marine larval fish","volume":"5","author":"Garrido","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_82","first-page":"42","article-title":"Environmental control on larval stages of fish subject to specific salinity range in tropical estuaries","volume":"13","author":"Santos","year":"2017","journal-title":"Reg. Stud. Mar. Sci."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.pocean.2017.10.006","article-title":"Temperature and food-mediated variability of European Atlantic sardine recruitment","volume":"159","author":"Garrido","year":"2017","journal-title":"Prog. Oceanogr."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1007\/s00442-004-1805-y","article-title":"Larval sensory abilities and mechanisms of habitat selection of a coral reef fish during settlement","volume":"143","author":"Lecchini","year":"2005","journal-title":"Oecologia"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Nagelkerken, I. (2009). Ecological Connectivity among Tropical Coastal Ecosystems, Springer.","DOI":"10.1007\/978-90-481-2406-0"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/jfb.13793","article-title":"Potential roles of smell and taste in the orientation behaviour of coral-reef fish larvae: Insights from morphology","volume":"95","author":"Hu","year":"2019","journal-title":"J. Fish Biol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ecolind.2015.03.005","article-title":"Do fish larvae have advantages over adults and other components for assessing estuarine ecological quality?","volume":"55","author":"Ramos","year":"2015","journal-title":"Ecol. Indic."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.ecolind.2015.05.044","article-title":"Assessing eutrophication in the Portuguese continental Exclusive Economic Zone within the European Marine Strategy Framework Directive","volume":"58","author":"Cabrita","year":"2015","journal-title":"Ecol. Indic."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.icesjms.2005.04.016","article-title":"Small-scale spatio-temporal variability in ichthyoplankton and zooplankton distribution in relation to a tidal-mixing front in the Irish Sea","volume":"62","author":"Lee","year":"2005","journal-title":"ICES J. Mar. Sci."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1146-609X(00)01078-X","article-title":"The distribution of estuarine fish larvae: Nutritional condition andco-occurrence with predators and prey","volume":"21","author":"Esteves","year":"2000","journal-title":"Acta Oecologica"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3354\/meps06978","article-title":"Linking behavioural ecology and oceanography: Larval behaviour determines growth, mortality and dispersal","volume":"347","author":"Fiksen","year":"2007","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Stanley, R., Snelgrove, P.V., Deyoung, B., and Gregory, R.S. (2012). Dispersal patterns, active behaviour, and flow environment during early life history of coastal cold water fishes. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046266"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.ecss.2016.10.022","article-title":"Biophysical processes leading to the ingress of temperate fish larvae into estuarine nursery areas: A review","volume":"183","author":"Paris","year":"2016","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.ecss.2016.06.022","article-title":"Bounded and unbounded boundaries\u2013Untangling mechanisms for estuarine-marine ecological connectivity: Scales of m to 10,000 km\u2013A review","volume":"198","author":"Wolanski","year":"2017","journal-title":"Estuar. Coast. Shelf Sci."}],"container-title":["Ecologies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2673-4133\/4\/2\/19\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:20:51Z","timestamp":1760124051000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2673-4133\/4\/2\/19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,21]]},"references-count":94,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["ecologies4020019"],"URL":"https:\/\/doi.org\/10.3390\/ecologies4020019","relation":{},"ISSN":["2673-4133"],"issn-type":[{"value":"2673-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,21]]}}}