{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T23:04:12Z","timestamp":1771023852484,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00081\/2020 (CIQUP)"],"award-info":[{"award-number":["UIDB\/00081\/2020 (CIQUP)"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["AlleRiskAssess-PTDC\/BAA-AGR\/31720\/2017"],"award-info":[{"award-number":["AlleRiskAssess-PTDC\/BAA-AGR\/31720\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-032294"],"award-info":[{"award-number":["POCI-01-0145-FEDER-032294"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/105395\/2014"],"award-info":[{"award-number":["SFRH\/BPD\/105395\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/75026\/2010"],"award-info":[{"award-number":["SFRH\/BD\/75026\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrochem"],"abstract":"<jats:p>The design and synthesis of artificial receptors based on molecular imprinting (MI) technology for the development of a new MIP-based biosensor for detection of the stress biomarker \u03b1-amylase in human saliva in point-of-care (PoC) applications is described in this work. The portable electrochemical devices for monitoring \u03b1-amylase consists of cost-effective and disposable gold screen-printed electrodes (AuSPEs). To build the electrochemical device, the template biomolecule was firstly immobilized directly over the working area of the gold chip previously activated with a self-assembled monolayer (SAM) of cysteamine (CA). Then, pyrrole (Py) monomer was selected as building block of a polymeric network prepared by CV electropolymerization. After the electropolymerization process, the enzyme was removed from the polymer film in order to build the specific recognition sites for the target enzyme. The MIP biosensor showed a very wide linear concentration range (between 3.0 \u00d7 10\u22124 to 0.60 mg mL\u22121 in buffer solution and between 3.0 \u00d7 10\u22124 to 3.0 \u00d7 10\u22122 mg mL\u22121 in human saliva) and low detection levels were achieved (LOD &lt; 3.0 \u00d7 10\u22124 mg mL\u22121) using square wave voltammetry (SWV) as the electroanalytical technique.<\/jats:p>","DOI":"10.3390\/electrochem2030028","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T09:25:52Z","timestamp":1627291552000},"page":"427-438","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker \u03b1-Amylase in Point-of-Care Applications"],"prefix":"10.3390","volume":"2","author":[{"given":"T\u00e2nia S. C. R.","family":"Rebelo","sequence":"first","affiliation":[{"name":"CIQUP\u2014Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s\/n, 4169-007 Porto, Portugal"}]},{"given":"In\u00eas M.","family":"Miranda","sequence":"additional","affiliation":[{"name":"CIQUP\u2014Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3291-7713","authenticated-orcid":false,"given":"Ana T. S. C.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"CIQUP\u2014Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8316-6406","authenticated-orcid":false,"given":"Laura I. G.","family":"Sousa","sequence":"additional","affiliation":[{"name":"CIQUP\u2014Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6421-9763","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"CIQUP\u2014Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s\/n, 4169-007 Porto, Portugal"}]},{"given":"Ant\u00f3nio F.","family":"Silva","sequence":"additional","affiliation":[{"name":"CIQUP\u2014Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8392-9581","authenticated-orcid":false,"given":"Carlos M.","family":"Pereira","sequence":"additional","affiliation":[{"name":"CIQUP\u2014Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s\/n, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.freeradbiomed.2015.04.005","article-title":"Salivary biomarkers of oxidative stress: A critical review","volume":"85","author":"Wang","year":"2015","journal-title":"Free Radic. 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