{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:16:56Z","timestamp":1760059016111,"version":"build-2065373602"},"reference-count":79,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T00:00:00Z","timestamp":1747699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\/MCTES","award":["2023.00033.RESTART","10.54499\/2022.02340.PTDC","LA\/P\/0094\/2020"],"award-info":[{"award-number":["2023.00033.RESTART","10.54499\/2022.02340.PTDC","LA\/P\/0094\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environments"],"abstract":"<jats:p>Nanoplastics (NPs) pose a significant environmental threat due to their small sizes, widespread distribution, and bioavailability, enabling interactions with marine organisms from pelagic to benthic species. In this study, the effects of 10 days of exposure to waterborne poly(methyl)methacrylate (PMMA) NPs were evaluated in the crab Carcinus maenas by assessing behavioral and biochemical endpoints (in gills, hepatopancreas, muscle, and hemolymph). Behavioral assessments using an open field test revealed that exposure to PMMA NPs resulted in an increase in distance walked (from 73.662 \u00b1 17.137 cm in control to 248.560 \u00b1 25.462 cm in the highest PMMA NPs concentration) and in random movement patterns. Muscle acetylcholinesterase (AChE) activity decreased from 10.83 \u00b1 0.73 to 6.75 \u00b1 0.45 nmol\/min\/mg of protein with PMMA NPs concentration increase, which, combined with behavioral responses, suggests neurological incapacities. In the gills and hepatopancreas, defense and detoxification mechanisms were activated, with a significant increase in superoxide dismutase (SOD) activity (at 20 \u00b5g\/L in gills and 80 \u00b5g\/L in hepatopancreas) and glutathione S-transferases (GSTs) activity (all PMMA NPs concentrations in gills and 20 and 320 \u00b5g\/L in hepatopancreas). Despite these activations, oxidative damage was observed, with a significant increase in protein carbonylation (PC) levels (20, 80, and 320 \u00b5g\/L in gills and 5, 20, and 80 \u00b5g\/L in hepatopancreas) and lipid peroxidation (LPO) (80 and 320 \u00b5g\/L in gills and 80 \u00b5g\/L in hepatopancreas). Effects on hemolymph followed a pattern similar to those reported for gills and hepatopancreas. An increase in SOD hemolymph activity was observed in organisms exposed to 5 and 80 \u00b5g\/L, and GSTs activity increased in crabs exposed to 80 \u00b5g\/L. Oxidative damage in hemolymph was only detected through LPO at 5 and 320 \u00b5g\/L. Overall, this study showed that PMMA NPs induce biochemical alterations and damage in different tissues of C. maenas and affect its behavior with potential impacts at a population level.<\/jats:p>","DOI":"10.3390\/environments12050169","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T04:05:17Z","timestamp":1747800317000},"page":"169","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Do Waterborne Nanoplastics Affect the Shore Crab Carcinus maenas? A Case Study with Poly(methyl)methacrylate Particles"],"prefix":"10.3390","volume":"12","author":[{"given":"Beatriz","family":"Neves","sequence":"first","affiliation":[{"name":"Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5404-7718","authenticated-orcid":false,"given":"Miguel","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9485-141X","authenticated-orcid":false,"given":"Carolina","family":"Fraz\u00e3o","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5734-4021","authenticated-orcid":false,"given":"M\u00f3nica","family":"Almeida","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5600-2396","authenticated-orcid":false,"given":"Ricardo J. B.","family":"Pinto","sequence":"additional","affiliation":[{"name":"CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6763-2665","authenticated-orcid":false,"given":"Etelvina","family":"Figueira","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4406-1847","authenticated-orcid":false,"given":"Ad\u00edlia","family":"Pires","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e04709","DOI":"10.1016\/j.heliyon.2020.e04709","article-title":"Plastic pollution in the marine environment","volume":"6","author":"Thushari","year":"2020","journal-title":"Heliyon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.biocon.2009.11.021","article-title":"Science in support of ecosystem-based management for the US West Coast and beyond","volume":"143","author":"Lester","year":"2010","journal-title":"Biol. 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