{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:41:58Z","timestamp":1770914518736,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FEDER funds","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"FEDER funds","award":["UIDP\/04004\/2020"],"award-info":[{"award-number":["UIDP\/04004\/2020"]}]},{"name":"FEDER funds","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"FEDER funds","award":["UIDB\/00102\/2020"],"award-info":[{"award-number":["UIDB\/00102\/2020"]}]},{"name":"FEDER funds","award":["UIDP\/00102\/2020"],"award-info":[{"award-number":["UIDP\/00102\/2020"]}]},{"name":"FEDER funds","award":["CEECIND\/02082\/2017\/CP1460\/CT0004"],"award-info":[{"award-number":["CEECIND\/02082\/2017\/CP1460\/CT0004"]}]},{"name":"FEDER funds","award":["2023. 03527.BD"],"award-info":[{"award-number":["2023. 03527.BD"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["UIDP\/04004\/2020"],"award-info":[{"award-number":["UIDP\/04004\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["UIDB\/00102\/2020"],"award-info":[{"award-number":["UIDB\/00102\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["UIDP\/00102\/2020"],"award-info":[{"award-number":["UIDP\/00102\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["CEECIND\/02082\/2017\/CP1460\/CT0004"],"award-info":[{"award-number":["CEECIND\/02082\/2017\/CP1460\/CT0004"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["2023. 03527.BD"],"award-info":[{"award-number":["2023. 03527.BD"]}]},{"name":"FCT, the European Social Fund (ESF), under the Programa Demografia","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"FCT, the European Social Fund (ESF), under the Programa Demografia","award":["UIDP\/04004\/2020"],"award-info":[{"award-number":["UIDP\/04004\/2020"]}]},{"name":"FCT, the European Social Fund (ESF), under the Programa Demografia","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"FCT, the European Social Fund (ESF), under the Programa Demografia","award":["UIDB\/00102\/2020"],"award-info":[{"award-number":["UIDB\/00102\/2020"]}]},{"name":"FCT, the European Social Fund (ESF), under the Programa Demografia","award":["UIDP\/00102\/2020"],"award-info":[{"award-number":["UIDP\/00102\/2020"]}]},{"name":"FCT, the European Social Fund (ESF), under the Programa Demografia","award":["CEECIND\/02082\/2017\/CP1460\/CT0004"],"award-info":[{"award-number":["CEECIND\/02082\/2017\/CP1460\/CT0004"]}]},{"name":"FCT, the European Social Fund (ESF), under the Programa Demografia","award":["2023. 03527.BD"],"award-info":[{"award-number":["2023. 03527.BD"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Forests"],"abstract":"<jats:p>The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease and is considered an A2 quarantine organism by the European Plant Protection Organisation. In Europe, this nematode has been reported in Pinus pinaster, P. radiata, and P. nigra. In May 2024, severe wilting symptoms were observed in P. sylvestris trees at Serra da Lous\u00e3 (Coimbra, the central area of continental Portugal). Wood samples were collected from six wilted trees, and the presence of PWN was investigated. From these, B.\u00a0xylophilus specimens were detected in five out of the six trees. Species identification was performed based on species-specific morphological diagnostic characters, and this was confirmed by real-time PCR using species-specific primers targeting the B. xylophilus satellite DNA region. This study presents the first detection of B. xylophilus in P. sylvestris in Portugal and in Europe.<\/jats:p>","DOI":"10.3390\/f15091556","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T05:54:47Z","timestamp":1725429287000},"page":"1556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Bursaphelenchus xylophilus in Pinus sylvestris\u2014The First Report in Europe"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7405-8916","authenticated-orcid":false,"given":"Lu\u00eds","family":"Fonseca","sequence":"first","affiliation":[{"name":"Centre for Functional Ecology-CFE, Associate Laboratory for Sustainable Land Use and Ecosystem Services-TERRA, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"},{"name":"FitoLab\u2014Laboratory for Phytopathology, Instituto Pedro Nunes (IPN), 3030-199 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0046-4716","authenticated-orcid":false,"given":"Hugo","family":"Silva","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology-CFE, Associate Laboratory for Sustainable Land Use and Ecosystem Services-TERRA, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6594-4892","authenticated-orcid":false,"given":"Joana M. S.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology-CFE, Associate Laboratory for Sustainable Land Use and Ecosystem Services-TERRA, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4944-6789","authenticated-orcid":false,"given":"Iv\u00e2nia","family":"Esteves","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology-CFE, Associate Laboratory for Sustainable Land Use and Ecosystem Services-TERRA, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1616-3632","authenticated-orcid":false,"given":"Carla","family":"Maleita","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology-CFE, Associate Laboratory for Sustainable Land Use and Ecosystem Services-TERRA, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"},{"name":"Chemical Engineering and Renewable Resources for Sustainability (CERES), Department of Chemical Engineering, University of Coimbra, 3030-790 Coimbra, Portugal"}]},{"given":"S\u00f3nia","family":"Lopes","sequence":"additional","affiliation":[{"name":"Institute for Nature Conservation and Forests (ICNF, IP), 3200-219 Lous\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8761-2151","authenticated-orcid":false,"given":"Isabel","family":"Abrantes","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology-CFE, Associate Laboratory for Sustainable Land Use and Ecosystem Services-TERRA, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,4]]},"reference":[{"key":"ref_1","unstructured":"EPPO (2024, May 27). 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