{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T21:23:06Z","timestamp":1768684986427,"version":"3.49.0"},"reference-count":118,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:00:00Z","timestamp":1722384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Rio Grande do Sul\u2014FAPERGS","award":["23\/2551-0000170-5"],"award-info":[{"award-number":["23\/2551-0000170-5"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Rio Grande do Sul\u2014FAPERGS","award":["88882.461702\/2019-01"],"award-info":[{"award-number":["88882.461702\/2019-01"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Rio Grande do Sul\u2014FAPERGS","award":["150197\/2023-3"],"award-info":[{"award-number":["150197\/2023-3"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014CAPES","award":["23\/2551-0000170-5"],"award-info":[{"award-number":["23\/2551-0000170-5"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014CAPES","award":["88882.461702\/2019-01"],"award-info":[{"award-number":["88882.461702\/2019-01"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014CAPES","award":["150197\/2023-3"],"award-info":[{"award-number":["150197\/2023-3"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2014CNPq","award":["23\/2551-0000170-5"],"award-info":[{"award-number":["23\/2551-0000170-5"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2014CNPq","award":["88882.461702\/2019-01"],"award-info":[{"award-number":["88882.461702\/2019-01"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2014CNPq","award":["150197\/2023-3"],"award-info":[{"award-number":["150197\/2023-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fermentation"],"abstract":"<jats:p>The distinct microbiological and physicochemical characteristics of sheep milk enable the production of cheeses with unique organoleptic properties. The scenario of sheep cheeses in Brazil is still uncertain, lacking data and regulations. This study aims to characterize the diversity of bacterial groups with potential pathogenic and\/or harmful properties to milk technology and correlate the presence of these bacteria with the physicochemical properties of sheep cheese. Additionally, it investigates the presence of virulence genes, resistance genes, and toxins in reference genomes. The main steps were (a) sample preparation and fractionation; (b) physicochemical analysis; (c) analysis of the free fatty acids profile by GC-FID; (d) analysis of the volatile compounds profile by GC-MS; (e) molecular analysis\u2014Next Generation Sequencing of the 16S rRNA gene (V3-V4 region); and (f) in silico analysis\u2014Genomics. A total of 1,061,392 sequences were identified, corresponding to 45 genera and 57 species. Of the total abundance, 95.7% were considered beneficial, while 3.9% were classified as bacteria with pathogenic potential (BPP) and\/or bacteria harmful to milk technology (BHMT). Five phyla were identified: Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota, and Fusobacteriota. The genera Acinetobacter, Pseudomonas, and Staphylococcus stood out in the BPP and BHMT groups, showing higher abundance. Alpha diversity of the cheese samples showed that the cheese origin significantly influences the richness, evenness, and abundance of bacterial species. Some physicochemical parameters, fatty acids, and volatile organic compounds showed a negative correlation with Staphylococcus. Reference genomes of two species exhibited a higher number of resistance and virulence genes. This reinforces the need to monitor bacteria considered of lesser relevance to prevent the transmission, through food, of potentially resistant and virulent pathogens.<\/jats:p>","DOI":"10.3390\/fermentation10080397","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T17:16:49Z","timestamp":1722446209000},"page":"397","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Pathogenic and Harmful Bacteria in Dairy Technology: Genomic Characterization and Its Correlation with Physicochemical Parameters of Sheep\u2019s Cheese Sold in Southern Brazil"],"prefix":"10.3390","volume":"10","author":[{"given":"Matheus Abra\u00e3o Piovesan","family":"Pedroso","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7256-265X","authenticated-orcid":false,"given":"Wemerson de Castro","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil"},{"name":"Department of Teaching, Research and Extension, Federal Institute of Education, Science and Technology Sul-rio-Grandense, Lajeado 95910-016, Brazil"},{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8813-8850","authenticated-orcid":false,"given":"Andrei Giacchetto","family":"Felice","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology and Bioinformatics, Department of Microbiology, Immunology and Parasitology, Federal University of Tri\u00e2ngulo Mineiro, Uberaba 38025-180, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4320-2318","authenticated-orcid":false,"given":"Sara Cristina Silva","family":"Cunha","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Bromatology and Hydrology Laboratory, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6767-6596","authenticated-orcid":false,"given":"Maria Beatriz Prior Pinto","family":"Oliveira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Neila Silvia Pereira dos Santos","family":"Richards","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,31]]},"reference":[{"key":"ref_1","unstructured":"(2024, July 23). FAOSTAT Food and Agriculture Organization of the United Nations. Available online: https:\/\/www.fao.org\/faostat\/en\/#data\/QCL\/visualize."},{"key":"ref_2","unstructured":"Bianchi, A.E., Monteiro, A.L.G., Morais, O.R.d., Beltr\u00e3o, R.B.F., and Debortoli, E.C. (2024, July 23). Caracteriza\u00e7\u00e3o dos sistemas produtivos de ovinos de leite no Brasil. MilkPoint. 2016, Se\u00e7\u00e3o Radar T\u00e9cnico, Ovinos e Caprinos, 1-5. Available online: http:\/\/www.infoteca.cnptia.embrapa.br\/infoteca\/handle\/doc\/1073171."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1541-4337.2011.00163.x","article-title":"Nutritional Value and Technological Suitability of Milk from Various Animal Species Used for Dairy Production","volume":"10","author":"Szwajkowska","year":"2011","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2132","DOI":"10.3168\/jds.2021-20961","article-title":"Composition and Aptitude for Cheese-Making of Milk from Cows, Buffaloes, Goats, Sheep, Dromedary Camels, and Donkeys","volume":"105","author":"Bittante","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e16418","DOI":"10.1111\/jfpp.16418","article-title":"Sodium Reduction in \u201cRequeij\u00e3o Cremoso\u201d Processed Cheese Made from Fresh and Refrigerated Sheep Milk","volume":"46","author":"Dalmina","year":"2022","journal-title":"J. Food Process. Preserv."},{"key":"ref_6","first-page":"648","article-title":"Goats Dairy Products as a Potentially Functional Food","volume":"11","author":"Hassan","year":"2014","journal-title":"Life Sci. J."},{"key":"ref_7","first-page":"8","article-title":"Perfil de textura de Labneh (iogurte grego)","volume":"64","author":"Ramos","year":"2009","journal-title":"Rev. Inst. Latic\u00ednios C\u00e2ndido Tostes"},{"key":"ref_8","unstructured":"Robinson, R.K., and Tamime, A.Y. (1996). Feta & Related Cheeses, CRC Press."},{"key":"ref_9","unstructured":"Furtado, M.M. (2005). Quesos T\u00edpicos de Latinoam\u00e9rica, Danisco."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.idairyj.2004.07.018","article-title":"Effect of the Use of Three Different Lamb Paste Rennets on Lipolysis of the PDO Pecorino Romano Cheese","volume":"15","author":"Addis","year":"2005","journal-title":"Int. Dairy J."},{"key":"ref_11","unstructured":"Nespolo, C.R., Bianchi, A.E., Queir\u00f3s, A.A.E., and Farinatti, L.H.E. (2012). A Produ\u00e7\u00e3o de Leite Ovino e Seus Derivados No Oeste Catarinense: Uma Alternativa Para o Produtor e Para o Consumidor. Anais do Simp\u00f3sio de Seguran\u00e7a Alimentar, FAURGS."},{"key":"ref_12","first-page":"55","article-title":"Estrat\u00e9gias e Inova\u00e7\u00f5es No Mercado L\u00e1cteo: Uma An\u00e1lise Dos Produtos de Leite de Ovelha No Sul Do Brasil","volume":"157","author":"Pedroso","year":"2023","journal-title":"Fazer Ci\u00eancia"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"103534","DOI":"10.1016\/j.jprot.2019.103534","article-title":"Milk Microbiota: Characterization Methods and Role in Cheese Production","volume":"210","author":"Tilocca","year":"2020","journal-title":"J. Proteom."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Paszczyk, B., and \u0141uczy\u0144ska, J. (2020). The Comparison of Fatty Acid Composition and Lipid Quality Indices in Hard Cow, Sheep, and Goat Cheeses. Foods, 9.","DOI":"10.3390\/foods9111667"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s42770-019-00182-3","article-title":"Technological Properties of Lactococcus Lactis Subsp. Lactis Bv. Diacetylactis Obtained from Dairy and Non-Dairy Niches","volume":"51","author":"Fusieger","year":"2020","journal-title":"Braz. J. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7373","DOI":"10.3168\/jds.2014-8396","article-title":"Volatile Compounds and Sensory Properties of Montasio Cheese Made from the Milk of Simmental Cows Grazing on Alpine Pastures","volume":"97","author":"Bovolenta","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s00217-002-0629-4","article-title":"Analytical Methods for the Determination of the Geographic Origin of Emmental Cheese: Volatile Compounds by GC\/MS-FID and Electronic Nose","volume":"216","author":"Pillonel","year":"2003","journal-title":"Eur. Food Res. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Possas, A., Bonilla-Luque, O.M., and Valero, A. (2021). From Cheese-Making to Consumption: Exploring the Microbial Safety of Cheeses through Predictive Microbiology Models. Foods, 10.","DOI":"10.3390\/foods10020355"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.35172\/rvz.2021.v28.475","article-title":"Metagenomic investigation in colonial cheese produced with pasteurized milk and sold in the state of Rio Grande do Sul, Brazil","volume":"28","author":"Remor","year":"2021","journal-title":"Veterin\u00e1ria Zootec."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Amato, E., Filipello, V., Gori, M., Lomonaco, S., Losio, M.N., Parisi, A., Huedo, P., Knabel, S.J., and Pontello, M. (2017). Identification of a Major Listeria Monocytogenes Outbreak Clone Linked to Soft Cheese in Northern Italy\u20142009\u20132011. BMC Infect. Dis., 17.","DOI":"10.1186\/s12879-017-2441-6"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.3168\/jds.2019-17138","article-title":"Virulence Factors and Antimicrobial Resistance of Staphylococcus aureus Isolated from the Production Process of Minas Artisanal Cheese from the Region of Campo Das Vertentes, Brazil","volume":"103","author":"Castro","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Alotaibi, K., and Khan, A.A. (2023). Prevalence and Molecular Characterization of Shiga Toxin-Producing Escherichia coli from Food and Clinical Samples. Pathogens, 12.","DOI":"10.3390\/pathogens12111302"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1111\/1541-4337.12929","article-title":"A Systematized Review and Qualitative Synthesis of Potential Risk Factors Associated with the Occurrence of Non-O157 Shiga Toxin-Producing Escherichia coli (STEC) in the Primary Production of Cattle","volume":"21","author":"Withenshaw","year":"2022","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Pinedo, L.C., Mughini-Gras, L., Franz, E., Hald, T., and Pires, S.M. (2022). Sources and Trends of Human Salmonellosis in Europe, 2015\u20132019: An Analysis of Outbreak Data. Int. J. Food Microbiol., 379.","DOI":"10.1016\/j.ijfoodmicro.2022.109850"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Primavilla, S., Roila, R., Rocchegiani, E., Blasi, G., Petruzzelli, A., Gabucci, C., Ottaviani, D., Di Lullo, S., Branciari, R., and Ranucci, D. (2023). Assessment of the Microbiological Safety and Hygiene of Raw and Thermally Treated Milk Cheeses Marketed in Central Italy between 2013 and 2020. Life, 13.","DOI":"10.3390\/life13122324"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5717","DOI":"10.1128\/AEM.00918-12","article-title":"High-Throughput Sequencing for Detection of Subpopulations of Bacteria Not Previously Associated with Artisanal Cheeses","volume":"78","author":"Quigley","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Regecov\u00e1, I., V\u00fdrostkov\u00e1, J., Zigo, F., Gregov\u00e1, G., and Kov\u00e1\u010dov\u00e1, M. (2021). Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep\u2019s Milk and Cheese. Antibiotics, 10.","DOI":"10.3390\/antibiotics10050570"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Danquah, C.A., Minkah, P.A.B., Duah-Junior, I.O., Amankwah, K.B., and Somuah, S.O. (2022). Antimicrobial Compounds from Microorganisms. Antibiotics, 11.","DOI":"10.3390\/antibiotics11030285"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Endres, C.M., Moreira, E., de Freitas, A.B., Castel, A.P.D., Graciano, F., Mann, M.B., Frazzon, A.P.G., Mayer, F.Q., and Frazzon, J. (2023). Evaluation of Enterotoxins and Antimicrobial Resistance in Microorganisms Isolated from Raw Sheep Milk and Cheese: Ensuring the Microbiological Safety of These Products in Southern Brazil. Microorganisms, 11.","DOI":"10.3390\/microorganisms11061618"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"341401","DOI":"10.1016\/j.aca.2023.341401","article-title":"Bioluminescence Resonance Energy Transfer Biosensor for Measuring Activity of a Protease Secreted by Pseudomonas fluorescens Growing in Milk","volume":"1270","author":"Dacres","year":"2023","journal-title":"Anal. Chim. Acta"},{"key":"ref_31","first-page":"107216","article-title":"The Prc and CtpA Proteases Modulate Cell-Surface Signaling Activity and Virulence in Pseudomonas aeruginosa","volume":"26","author":"Bastiaansen","year":"2023","journal-title":"Science"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s12223-024-01131-w","article-title":"The Role of Bacteria in Gallstone Formation","volume":"69","author":"Wang","year":"2024","journal-title":"Folia Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Parija, S.C. (2023). Pseudomonas, Burkholderia and Acinetobacter. Textbook of Microbiology and Immunology, Springer Nature.","DOI":"10.1007\/978-981-19-3315-8_38"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8183","DOI":"10.1128\/AEM.02601-15","article-title":"Application of Metagenomic Sequencing to Food Safety: Detection of Shiga Toxin-Producing Escherichia coli on Fresh Bagged Spinach","volume":"81","author":"Leonard","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1038\/nmeth.2066","article-title":"Metagenomic Microbial Community Profiling Using Unique Clade-Specific Marker Genes","volume":"9","author":"Segata","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Erhardt, M.M., Oliveira, W.d.C., Fr\u00f6der, H., Marques, P.H., Oliveira, M.B.P.P., and Richards, N.S.P.d.S. (2023). Lactic Bacteria in Artisanal Cheese: Characterization through Metagenomics. Fermentation, 9.","DOI":"10.3390\/fermentation9010041"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3705","DOI":"10.1111\/1462-2920.16022","article-title":"Metagenomic Tracking of Antibiotic Resistance Genes through a Pre-Harvest Vegetable Production System: An Integrated Lab-, Microcosm- and Greenhouse-Scale Analysis","volume":"24","author":"Keenum","year":"2022","journal-title":"Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"108559","DOI":"10.1016\/j.foodcont.2021.108559","article-title":"Isolation of AmpC- and Extended Spectrum \u03b2-Lactamase-Producing Enterobacterales from Fresh Vegetables in the United States","volume":"132","author":"Moon","year":"2022","journal-title":"Food Control"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e09089","DOI":"10.1016\/j.heliyon.2022.e09089","article-title":"Assessing Bacterial Diversity and Antibiotic Resistance Dynamics in Wastewater Effluent-Irrigated Soil and Vegetables in a Microcosm Setting","volume":"8","author":"Onalenna","year":"2022","journal-title":"Heliyon"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"112202","DOI":"10.1016\/j.foodres.2022.112202","article-title":"Evidence of Virulence and Antibiotic Resistance Genes from the Microbiome Mapping in Minimally Processed Vegetables Producing Facilities","volume":"162","author":"Valentino","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"111190","DOI":"10.1016\/j.foodres.2022.111190","article-title":"New Insights of Bacterial Communities in Fermented Vegetables from Shotgun Metagenomics and Identification of Antibiotic Resistance Genes and Probiotic Bacteria","volume":"157","author":"Yasir","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Yu, Y., Li, L., Xu, Y., Li, H., Yu, Y., and Xu, Z. (2022). Metagenomics Reveals the Microbial Community Responsible for Producing Biogenic Amines During Mustard [Brassica Juncea (L.)] Fermentation. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.824644"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1111\/1471-0307.12944","article-title":"Bacterial and Fungal Microbiota of Mould-Ripened Cheese Produced in Konya","volume":"76","author":"Yurt","year":"2023","journal-title":"Int. J. Dairy Technol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Papadakis, P., Konteles, S., Batrinou, A., Ouzounis, S., Tsironi, T., Halvatsiotis, P., Tsakali, E., Van Impe, J.F.M., Vougiouklaki, D., and Strati, I.F. (2021). Characterization of Bacterial Microbiota of P.D.O. Feta Cheese by 16S Metagenomic Analysis. Microorganisms, 9.","DOI":"10.3390\/microorganisms9112377"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Masenya, K., Manganyi, M.C., and Dikobe, T.B. (2024). Exploring Cereal Metagenomics: Unravelling Microbial Communities for Improved Food Security. Microorganisms, 12.","DOI":"10.3390\/microorganisms12030510"},{"key":"ref_46","first-page":"e000409","article-title":"Metagenomic Approaches in Microbial Ecology: An Update on Whole-Genome and Marker Gene Sequencing Analyses","volume":"6","author":"Buchrieser","year":"2020","journal-title":"Microb. Genom."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.copbio.2021.01.019","article-title":"Metagenomic Tools in Microbial Ecology Research","volume":"67","author":"Li","year":"2021","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.tifs.2018.06.010","article-title":"Drivers That Establish and Assembly the Lactic Acid Bacteria Biota in Cheeses","volume":"78","author":"Gobbetti","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Lemes, M.R., Rodrigues, T.C.V., Jaiswal, A.K., Tiwari, S., Sales-Campos, H., Andrade-Silva, L.E., Oliveira, C.J.F., Azevedo, V., Rodrigues, V., and Soares, S.C. (2022). In Silico Designing of a Recombinant Multi-Epitope Antigen for Leprosy Diagnosis. J. Genet. Eng. Biotechnol., 20.","DOI":"10.1186\/s43141-022-00411-7"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"13950","DOI":"10.1073\/pnas.0506758102","article-title":"Genome Analysis of Multiple Pathogenic Isolates of Streptococcus agalactiae: Implications for the Microbial \u201cPan-Genome\u201d","volume":"102","author":"Tettelin","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/ddr.20413","article-title":"In Silico Subtractive Genomics for Target Identification in Human Bacterial Pathogens","volume":"72","author":"Barh","year":"2011","journal-title":"Drug Dev. Res."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Lux, T.M., Lee, R., and Love, J. (2014). Genome-Wide Phylogenetic Analysis of the Pathogenic Potential of Vibrio furnissii. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00435"},{"key":"ref_53","unstructured":"IAL Instituto Adolfo Lutz (1985). Normas Anal\u00edticas Do Instituto Adolfo Lutz, IMESP. [3rd ed.]."},{"key":"ref_54","unstructured":"IAL Instituto Adolfo Lutz (2008). M\u00e9todos F\u00edsico-Qu\u00edmicos Para An\u00e1lise de Alimentos, ANVISA."},{"key":"ref_55","unstructured":"Tontisirin, K., MacLean, W.C., and Warwick, P. (2003). Food Energy: Methods of Analysis and Conversion Factors: Report of a Technical Workshop, Rome, 3\u20136 December 2002, FAO."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A Rapid Method of Total Lipid Extraction and Purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_57","first-page":"475","article-title":"Rapid Preparation of Fatty Acid Methyl Esters from Lipids","volume":"22","author":"Hartman","year":"1973","journal-title":"Lab. Pract."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1590\/S0100-40422012000200008","article-title":"Aspectos anal\u00edticos da resposta do detector de ioniza\u00e7\u00e3o em chama para \u00e9steres de \u00e1cidos graxos em biodiesel e alimentos","volume":"35","author":"Visentainer","year":"2012","journal-title":"Qu\u00edm. Nova"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"136239","DOI":"10.1016\/j.foodchem.2023.136239","article-title":"Volatolomic Approach by HS-SPME\/GC\u2013MS and Chemometric Evaluations for the Discrimination of X-Ray Irradiated Mozzarella Cheese","volume":"423","author":"Zianni","year":"2023","journal-title":"Food Chem."},{"key":"ref_60","unstructured":"(2024, June 16). NIST NIST V2.2 (National Institute of Standards and Technology, USA) Library Database, Available online: https:\/\/webbook.nist.gov\/chemistry\/name-ser\/."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"DePaula, J., Cunha, S.C., Cruz, A., Sales, A.L., Revi, I., Fernandes, J., Ferreira, I.M.P.L.V.O., Miguel, M.A.L., and Farah, A. (2022). Volatile Fingerprinting and Sensory Profiles of Coffee Cascara Teas Produced in Latin American Countries. Foods, 11.","DOI":"10.3390\/foods11193144"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"110801","DOI":"10.1016\/j.foodres.2021.110801","article-title":"Headspace Volatolome of Peel Flours from Citrus Fruits Grown in Brazil","volume":"150","author":"Cunha","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v021.i12","article-title":"Reshaping Data with the Reshape Package","volume":"21","author":"Wickham","year":"2007","journal-title":"J. Stat. Softw."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"McMurdie, P.J., and Holmes, S. (2013). Phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0061217"},{"key":"ref_66","unstructured":"Oksanen, J., Simpson, G.L., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., O\u2019Hara, R.B., Solymos, P., Stevens, M.H.H., and Szoecs, E. (2024, July 23). Vegan: Community Ecology Package 2024. Available online: https:\/\/cran.r-project.org\/web\/packages\/vegan\/index.html."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: Multiple Sequence Alignment with High Accuracy and High Throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Mori, H., Maruyama, T., Yano, M., Yamada, T., and Kurokawa, K. (2018). VITCOMIC2: Visualization Tool for the Phylogenetic Composition of Microbial Communities Based on 16S rRNA Gene Amplicons and Metagenomic Shotgun Sequencing. BMC Syst. Biol., 12.","DOI":"10.1186\/s12918-018-0545-2"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"D733","DOI":"10.1093\/nar\/gkv1189","article-title":"Reference Sequence (RefSeq) Database at NCBI: Current Status, Taxonomic Expansion, and Functional Annotation","volume":"44","author":"Wright","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Agren, J., Sundstr\u00f6m, A., H\u00e5fstr\u00f6m, T., and Segerman, B. (2012). Gegenees: Fragmented Alignment of Multiple Genomes for Determining Phylogenomic Distances and Genetic Signatures Unique for Specified Target Groups. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0039107"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Rodrigues, D.L.N., Ariute, J.C., Rodrigues da Costa, F.M., Benko-Iseppon, A.M., Barh, D., Azevedo, V., and Aburjaile, F. (2023). PanViTa: Pan Virulence and resistance Analysis. Front. Bioinform., 3.","DOI":"10.3389\/fbinf.2023.1070406"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Bennato, F., Di Domenico, M., Ianni, A., Di Gialleonardo, L., Camm\u00e0, C., and Martino, G. (2022). Grape Pomace in Ewes Diet Affects Metagenomic Profile, Volatile Compounds and Biogenic Amines Contents of Ripened Cheese. Fermentation, 8.","DOI":"10.3390\/fermentation8110598"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Kamilari, E., Anagnostopoulos, D.A., Papademas, P., Efthymiou, M., Tretiak, S., and Tsaltas, D. (2020). Snapshot of Cyprus Raw Goat Milk Bacterial Diversity via 16S rDNA High-Throughput Sequencing; Impact of Cold Storage Conditions. Fermentation, 6.","DOI":"10.3390\/fermentation6040100"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"109298","DOI":"10.1016\/j.lwt.2020.109298","article-title":"Characterizing Halloumi Cheese\u2019s Bacterial Communities through Metagenomic Analysis","volume":"126","author":"Kamilari","year":"2020","journal-title":"LWT"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Kamimura, B.A., Cabral, L., Noronha, M.F., Baptista, R.C., Nascimento, H.M., and Sant\u2019Ana, A.S. (2020). Amplicon Sequencing Reveals the Bacterial Diversity in Milk, Dairy Premises and Serra Da Canastra Artisanal Cheeses Produced by Three Different Farms. Food Microbiol., 89.","DOI":"10.1016\/j.fm.2020.103453"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"111579","DOI":"10.1016\/j.lwt.2021.111579","article-title":"Molecular Characterization of the Bacterial Communities Present in Sheep\u2019s Milk and Cheese Produced in South Brazilian Region via 16S rRNA Gene Metabarcoding Sequencing","volume":"147","author":"Endres","year":"2021","journal-title":"LWT"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.ijfoodmicro.2017.11.004","article-title":"Microbial Diversity of Consumption Milk during Processing and Storage","volume":"266","author":"Porcellato","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"114483","DOI":"10.1016\/j.foodres.2024.114483","article-title":"Metagenomic and Flavoromic Profiling Reveals the Correlation between the Microorganisms and Volatile Flavor Compounds in Monascus-Fermented Cheese","volume":"188","author":"Wang","year":"2024","journal-title":"Food Res. Int."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"104561","DOI":"10.1016\/j.idairyj.2019.104561","article-title":"A Review on Microbiological and Technological Aspects of Serpa PDO Cheese: An Ovine Raw Milk Cheese","volume":"100","author":"Tavaria","year":"2020","journal-title":"Int. Dairy J."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1071\/EA96014","article-title":"The Quality of Sheep Milk: A Review","volume":"37","author":"Bencini","year":"1997","journal-title":"Aust. J. Exp. Agric."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ijgfs.2011.11.003","article-title":"Defining Microbial Terroir: The Use of Native Fungi for the Study of Traditional Fermentative Processes","volume":"1","author":"Felder","year":"2012","journal-title":"Int. J. Gastron. Food Sci."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Xu, Z.S., Ju, T., Yang, X., and G\u00e4nzle, M. (2023). A Meta-Analysis of Bacterial Communities in Food Processing Facilities: Driving Forces for Assembly of Core and Accessory Microbiomes across Different Food Commodities. Microorganisms, 11.","DOI":"10.3390\/microorganisms11061575"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Yuan, H., Han, S., Zhang, S., Xue, Y., Zhang, Y., Lu, H., and Wang, S. (2022). Microbial Properties of Raw Milk throughout the Year and Their Relationships to Quality Parameters. Foods, 11.","DOI":"10.3390\/foods11193077"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Rzewuska, M., Kwiecie\u0144, E., Chrobak-Chmiel, D., Kizerwetter-\u015awida, M., Stefa\u0144ska, I., and Giery\u0144ska, M. (2019). Pathogenicity and Virulence of Trueperella pyogenes: A Review. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20112737"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ijid.2016.02.014","article-title":"Emerging Role of Raoultella ornithinolytica in Human Infections: A Series of Cases and Review of the Literature","volume":"45","author":"Seng","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1080\/22221751.2019.1634981","article-title":"Klebsiella variicola: An Emerging Pathogen in Humans","volume":"8","year":"2019","journal-title":"Emerg. Microbes Infect."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.anl.2011.07.002","article-title":"Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in Children with Otitis Media with Effusion (OME) in Iranian Patients","volume":"39","author":"Khoramrooz","year":"2012","journal-title":"Auris Nasus Larynx"},{"key":"ref_90","unstructured":"Lima, B.B., and Leal, M.C. (2017). Par\u00e2metros Indicadores de Qualidade de Queijos Artesanais Comercializados Em Castro-PR, Universidade Federal do Paran\u00e1."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3168\/jds.2018-15247","article-title":"Effect of a Whey Protein and Rapeseed Oil Gel Feed Supplement on Milk Fatty Acid Composition of Holstein Cows","volume":"102","author":"Kliem","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"103955","DOI":"10.1016\/j.jfca.2021.103955","article-title":"A Large Survey of the Fatty Acid Profile and Gross Composition of Brazilian Artisanal Cheeses","volume":"101","author":"Margalho","year":"2021","journal-title":"J. Food Compos. Anal."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"e13454","DOI":"10.1111\/asj.13454","article-title":"Effects of Ear Corn Silage Supplementation on Milk Production and Milk Fatty Acid Profiles in Grazing Dairy Farms","volume":"91","author":"Mitani","year":"2020","journal-title":"Anim. Sci. J."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Nudda, A., Cannas, A., Correddu, F., Atzori, A.S., Lunesu, M.F., Battacone, G., and Pulina, G. (2020). Sheep and Goats Respond Differently to Feeding Strategies Directed to Improve the Fatty Acid Profile of Milk Fat. Animals, 10.","DOI":"10.3390\/ani10081290"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.foodres.2017.05.018","article-title":"Effect of Sodium Reduction and Flavor Enhancer Addition on Probiotic Prato Cheese Processing","volume":"99","author":"Silva","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.foodchem.2017.12.064","article-title":"Partial Substitution of NaCl by KCl and Addition of Flavor Enhancers on Probiotic Prato Cheese: A Study Covering Manufacturing, Ripening and Storage Time","volume":"248","author":"Silva","year":"2018","journal-title":"Food Chem."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/raq.12320","article-title":"Applications of Microalga Chlorella vulgaris in Aquaculture","volume":"12","author":"Ahmad","year":"2020","journal-title":"Rev. Aquac."},{"key":"ref_98","unstructured":"Mostafa, S.S.M. (2012). Microalgal Biotechnology: Prospects and Applications. Plant Science, IntechOpen."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s43162-023-00265-6","article-title":"The Multidimensional Benefits of Eicosapentaenoic Acid: From Heart Health to Inflammatory Control","volume":"35","author":"Nassar","year":"2023","journal-title":"Egypt. J. Intern. Med."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/1999-3110-54-39","article-title":"Antimicrobial Activities of Stearidonic and Gamma-Linolenic Acids from the Green Seaweed Enteromorpha Linza against Several Oral Pathogenic Bacteria","volume":"54","author":"Park","year":"2013","journal-title":"Bot. Stud."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1080\/26388081.2020.1715256","article-title":"Chlorella vulgaris: A Perspective on Its Potential for Combining High Biomass with High Value Bioproducts","volume":"1","author":"Ru","year":"2020","journal-title":"Appl. Phycol."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Sukhikh, S., Prosekov, A., Ivanova, S., Maslennikov, P., Andreeva, A., Budenkova, E., Kashirskikh, E., Tcibulnikova, A., Zemliakova, E., and Samusev, I. (2022). Identification of Metabolites with Antibacterial Activities by Analyzing the FTIR Spectra of Microalgae. Life, 12.","DOI":"10.3390\/life12091395"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1590\/S0100-40422004000200020","article-title":"Queijos: Aspectos qu\u00edmicos, bioqu\u00edmicos e microbiol\u00f3gicos","volume":"27","author":"Perry","year":"2004","journal-title":"Qu\u00edmica Nova"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0958-6946(02)00124-3","article-title":"Key Odorants in Various Cheese Types as Determined by Gas Chromatography-Olfactometry","volume":"12","author":"Curioni","year":"2002","journal-title":"Int. Dairy J."},{"key":"ref_105","unstructured":"Cardoso, M.G.B. (2022). Microbiota bacteriana e compostos vol\u00e1teis de queijo Minas Artesanal da regi\u00e3o da Serra da Canastra, Universidade Federal de Lavras."},{"key":"ref_106","first-page":"3","article-title":"O Estufamento Tardio Dos Queijos: Caracter\u00edsticas e Preven\u00e7\u00e3o, Uma Revis\u00e3o","volume":"40","author":"Furtado","year":"1985","journal-title":"Rev. Inst. Latic\u00ednios C\u00e2ndido Tostes"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Vinayamohan, P.G., Viju, L.S., Joseph, D., and Venkitanarayanan, K. (2023). Fermented Foods as a Potential Vehicle of Antimicrobial-Resistant Bacteria and Genes. Fermentation, 9.","DOI":"10.20944\/preprints202307.0741.v1"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Gaurav, A., Bakht, P., Saini, M., Pandey, S., and Pathania, R. (2023). Role of Bacterial Efflux Pumps in Antibiotic Resistance, Virulence, and Strategies to Discover Novel Efflux Pump Inhibitors. Microbiology, 169.","DOI":"10.1099\/mic.0.001333"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"100911","DOI":"10.1016\/j.drup.2022.100911","article-title":"Evolution of RND Efflux Pumps in the Development of a Successful Pathogen","volume":"66","author":"Naidu","year":"2023","journal-title":"Drug Resist. Updates"},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Saini, N., Aamir, M., Singh, V.K., Deepak, B., and Mona, S. (2023). Unveiling the Microbial Diversity and Functional Dynamics of Shiv Kund, Sohna Hot Spring, India through a Shotgun Metagenomics Approach. Arch. Microbiol., 205.","DOI":"10.1007\/s00203-023-03664-z"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/s41579-022-00820-y","article-title":"Molecular Mechanisms of Antibiotic Resistance Revisited","volume":"21","author":"Darby","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_112","doi-asserted-by":"crossref","unstructured":"Gervasoni, S., Mehla, J., Bergen, C.R., Leus, I.V., Margiotta, E., Malloci, G., Bosin, A., Vargiu, A.V., Lomovskaya, O., and Rybenkov, V.V. (2023). Molecular Determinants of Avoidance and Inhibition of Pseudomonas aeruginosa MexB Efflux Pump. mBio, 14.","DOI":"10.1128\/mbio.01403-23"},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Zhou, J.-X., Feng, D.-Y., Li, X., Zhu, J.-X., Wu, W.-B., and Zhang, T. (2023). Advances in Research on Virulence Factors of Acinetobacter baumannii and Their Potential as Novel Therapeutic Targets. J. Appl. Microbiol., 134.","DOI":"10.1093\/jambio\/lxac089"},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Shadan, A., Pathak, A., Ma, Y., Pathania, R., and Singh, R.P. (2023). Deciphering the Virulence Factors, Regulation, and Immune Response to Acinetobacter baumannii Infection. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1053968"},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Fursova, N.K., Fursov, M.V., Astashkin, E.I., Fursova, A.D., Novikova, T.S., Kislichkina, A.A., Sizova, A.A., Fedyukina, G.N., Savin, I.A., and Ershova, O.N. (2023). Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Causing Nosocomial Meningitis in the Neurological Intensive Care Unit. Microorganisms, 11.","DOI":"10.3390\/microorganisms11082020"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"106252","DOI":"10.1016\/j.micpath.2023.106252","article-title":"Clinical Impact of the Type VI Secretion System on Clinical Characteristics, Virulence and Prognosis of Acinetobacter baumannii during Bloodstream Infection","volume":"182","author":"Lin","year":"2023","journal-title":"Microb. Pathog."},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Alam, S.A., and Saha, P. (2023). Chemotactic Response of P-Nitrophenol Degrading Pseudomonas asiatica Strain PNPG3 through Phenotypic and Genome Sequence-Based in Silico Studies. 3 Biotech, 13.","DOI":"10.1007\/s13205-023-03809-3"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"739874","DOI":"10.1016\/j.aquaculture.2023.739874","article-title":"Flagellar Gene fliP Contributes to the Virulence of Pseudomonas plecoglossicida by Regulating Its Motility","volume":"576","author":"He","year":"2023","journal-title":"Aquaculture"}],"container-title":["Fermentation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2311-5637\/10\/8\/397\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:27:21Z","timestamp":1760110041000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2311-5637\/10\/8\/397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,31]]},"references-count":118,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["fermentation10080397"],"URL":"https:\/\/doi.org\/10.3390\/fermentation10080397","relation":{},"ISSN":["2311-5637"],"issn-type":[{"value":"2311-5637","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,31]]}}}