{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:30:58Z","timestamp":1760059858589,"version":"build-2065373602"},"reference-count":78,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006162","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Pernambuco (FACEPE) through the Program Arranjos Produtivos Locais (APL) grant number","doi-asserted-by":"publisher","award":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"],"award-info":[{"award-number":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"]}],"id":[{"id":"10.13039\/501100006162","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006162","name":"Locus de Inova\u00e7\u00e3o 02\/2022","doi-asserted-by":"publisher","award":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"],"award-info":[{"award-number":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"]}],"id":[{"id":"10.13039\/501100006162","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"],"award-info":[{"award-number":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"]}]},{"name":"LABBELS\u2014Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems","award":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"],"award-info":[{"award-number":["APQ-0434-2.12\/20","APQ-0161-9.26\/22","UIDB\/04469\/2020","LA\/P\/0029\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fermentation"],"abstract":"<jats:p>The singular biodiversity of the Brazilian Caatinga inspires innovative solutions in food science. In this study, we evaluated the prebiotic potential of honeys produced by Apis mellifera in the Paje\u00fa hinterland, Pernambuco, Brazil (Caatinga Biome), with different floral origins: Mastic (Aroeira), Mesquite (Algaroba), and mixed flowers. These were used to formulate synbiotic and alcoholic beverages fermented by Saccharomyces boulardii CNCM I-745. Static and dynamic simulations of the human gastrointestinal tract (GIT) were used, as well as physicochemical, rheological, and microbiological analyses. The results revealed that honey positively influences the viability and resilience of probiotic yeast, especially honey with a predominance of Algaroba, which promoted the highest survival rate (&gt;89%) even after 28 days of refrigeration and in dynamic in vitro simulation of the GIT (more realistic to human physio-anatomical conditions). The phenolic composition of the honeys showed a correlation with this tolerance. The use of complementary methodologies, such as flow cytometry, validated the findings and highlighted the functional value of these natural matrices, revealing an even greater longevity potential compared to conventional microbiological methodology. The data reinforces the potential of the Caatinga as a source of bioactive and sustainable compounds, proposing honey as a promising non-dairy synbiotic vehicle. This work contributes to the appreciation of the biome and the development of functional food products with a positive social, economic, and ecological impact.<\/jats:p>","DOI":"10.3390\/fermentation11070405","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T09:45:52Z","timestamp":1752572752000},"page":"405","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Potential Prebiotic Effect of Caatinga Bee Honeys from the Paje\u00fa Hinterland (Pernambuco, Brazil) on Synbiotic Alcoholic Beverages Fermented by Saccharomyces boulardii CNCM I-745"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4222-7153","authenticated-orcid":false,"given":"Walter de Paula","family":"Pinto-Neto","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences, University of Pernambuco, Campus of Recife, Recife 50100-130, Brazil"},{"name":"Department of Genetics, Centre of Biosciences, Federal University of Pernambuco, Campus of Recife, Recife 50670-901, Brazil"},{"name":"Department of Rural Technology, Federal Rural University of Pernambuco, Campus of Recife, Recife 58050-900, Brazil"},{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Luis","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"given":"Raquel F. S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"given":"M\u00e1rcia Cristina Teixeira","family":"Marques","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Rui Miguel Martins","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7234-4409","authenticated-orcid":false,"given":"Lu\u00eds","family":"Abrunhosa","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7085-7048","authenticated-orcid":false,"given":"Aline Magalh\u00e3es","family":"de Barros","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Neide Kazue Sakugawa","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Department of Rural Technology, Federal Rural University of Pernambuco, Campus of Recife, Recife 58050-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8223-3742","authenticated-orcid":false,"given":"Ana Cristina","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3593-8878","authenticated-orcid":false,"given":"Antonio Augusto","family":"Vicente","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7462-7541","authenticated-orcid":false,"given":"Rafael Barros de","family":"Souza","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, University of Pernambuco, Campus of Recife, Recife 50100-130, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9727-0300","authenticated-orcid":false,"given":"Marcos Antonio de Morais","family":"Junior","sequence":"additional","affiliation":[{"name":"Department of Genetics, Centre of Biosciences, Federal University of Pernambuco, Campus of Recife, Recife 50670-901, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"ref_1","unstructured":"(2019). Codex Alimentarius: International Food StandardsFAO\/WHO Standard for Honey. (Standard No. CXS 12-1981)."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Pinto-Neto, W.d.P., Silva, R.K., Lima, B.d.S., Acioli, G.F.d.S., Paix\u00e3o, G.A.d., Muniz, B.C., Silva, P.K.N.d., Costa, R.M.P.B., Silva, F.S.B.d., and Melo, H.F.d. (2024). Bee Honey of the Paje\u00fa Hinterland, Pernambuco, Brazil: Physicochemical Characterization and Biological Activity. Food Biosci., 60.","DOI":"10.1016\/j.fbio.2024.104289"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3663","DOI":"10.1080\/14786419.2023.2254457","article-title":"Evaluation of the Skin Whitening and Antioxidant Activity of Myracrodruon urundeuva Extract (Aroeira-Do-Sert\u00e3o)","volume":"38","author":"Silveira","year":"2024","journal-title":"Nat. Prod. Res."},{"key":"ref_4","first-page":"5","article-title":"Mesquite Tree (Prosopis spp.): A Native Resource for the Potential for Human Consumption and Healthcare","volume":"80","year":"2024","journal-title":"Plant Foods Hum. Nutr. Dordr. Neth."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"23","DOI":"10.18378\/rvads.v10i5.3455","article-title":"Schinus terebinthifolius Raddi (Aroeira) e suas propriedades na Medicina Popular","volume":"10","author":"Oliveira","year":"2015","journal-title":"Rev. Verde Agroecol. E Desenvolv. Sustent\u00e1vel"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"144469","DOI":"10.1016\/j.foodchem.2025.144469","article-title":"Improved Quality, Sensory Properties and Nutraceutical Potential of the Fermented Beverages Fortified with Freeze-Dried Berries and Acacia Honey","volume":"486","author":"Ogrodowczyk","year":"2025","journal-title":"Food Chem."},{"key":"ref_7","unstructured":"FAO (2002). WHO Guidelines for the Evaluation of Probiotics in Food, FAO."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/nrgastro.2017.75","article-title":"Expert Consensus Document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) Consensus Statement on the Definition and Scope of Prebiotics","volume":"14","author":"Gibson","year":"2017","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Markowiak, P., and \u015ali\u017cewska, K. (2017). Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients, 9.","DOI":"10.3390\/nu9091021"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Mendon\u00e7a, A.A., Pinto-Neto, W.d.P., da Paix\u00e3o, G.A., Santos, D.d.S., De Morais, M.A., and De Souza, R.B. (2023). Journey of the Probiotic Bacteria: Survival of the Fittest. Microorganisms, 11.","DOI":"10.3390\/microorganisms11010095"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.3748\/wjg.v25.i18.2188","article-title":"Diversity of Saccharomyces Boulardii CNCM I-745 Mechanisms of Action against Intestinal Infections","volume":"25","author":"Czerucka","year":"2019","journal-title":"World J. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1007\/s00284-020-02053-9","article-title":"Saccharomyces Boulardii CNCM I-745: A Non-Bacterial Microorganism Used as Probiotic Agent in Supporting Treatment of Selected Diseases","volume":"77","author":"Ruszkowski","year":"2020","journal-title":"Curr. Microbiol."},{"key":"ref_13","first-page":"e46314","article-title":"Unique Properties of Yeast Probiotic Saccharomyces Boulardii CNCM I-745: A Narrative Review","volume":"15","author":"Gopalan","year":"2023","journal-title":"Cureus"},{"key":"ref_14","first-page":"237","article-title":"Saccharomyces Boulardii CNCM I-745 Supports Regeneration of the Intestinal Microbiota after Diarrheic Dysbiosis\u2014A Review","volume":"8","author":"Swidsinski","year":"2015","journal-title":"Clin. Exp. Gastroenterol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.medmal.2017.07.001","article-title":"Antibiotic Susceptibility of Probiotic Strains: Is It Reasonable to Combine Probiotics with Antibiotics?","volume":"47","author":"Neut","year":"2017","journal-title":"M\u00e9decine Mal. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1111\/jam.15467","article-title":"Probiotic Properties of Yeasts in Traditional Fermented Foods and Beverages","volume":"132","author":"Tamang","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_17","unstructured":"(2008). M\u00e9todos F\u00edsico-Qu\u00edmicos Para An\u00e1lise de Alimentos, Instituto Adolfo Lutz. [1st digital ed.]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of Protein Using Bicinchoninic Acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The Ferric Reducing Ability of Plasma (FRAP) as a Measure of \u201cAntioxidant Power\u201d: The FRAP Assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1134\/S1021443713050129","article-title":"Induction of Glycyrrhizin and Total Phenolic Compound Production in Licorice by Using Arbuscular Mycorrhizal Fungi","volume":"60","author":"Orujei","year":"2013","journal-title":"Russ. J. Plant Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jep.2008.07.032","article-title":"A New Approach to Study Medicinal Plants with Tannins and Flavonoids Contents from the Local Knowledge","volume":"120","author":"Alencar","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/00218839.1998.11100961","article-title":"Analysis of Propolis: Some Parameters and Procedures for Chemical Quality Control","volume":"37","author":"Woisky","year":"1998","journal-title":"J. Apic. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.sajb.2019.06.024","article-title":"Arbuscular Mycorrhizal Fungi Affect Total Phenolic Content and Antimicrobial Activity of Tamarix Gallica in Natural Semi-Arid Algerian Areas","volume":"125","author":"Bencherif","year":"2019","journal-title":"S. Afr. J. Bot."},{"key":"ref_24","unstructured":"AOAC International (2002). Official Methods of Analysis of AOAC International, AOAC International. [17th ed.]."},{"key":"ref_25","unstructured":"AOAC International (2005). Official Methods of Analysis of AOAC International, AOAC International. [18th ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.foodchem.2012.10.147","article-title":"Influence of Nitrogen Supply on the Production of Higher Alcohols\/Esters and Expression of Flavour-Related Genes in Cacha\u00e7a Fermentation","volume":"138","author":"Vidal","year":"2013","journal-title":"Food Chem."},{"key":"ref_27","first-page":"47","article-title":"Biosynthesis of Higher Alcohol Flavour Compounds by the Yeast Saccharomyces Cerevisiae: Impact of Oxygen Availability and Responses to Glucose Pulse in Minimal Growth Medium with Leucine as Sole Nitrogen Source","volume":"32","year":"2015","journal-title":"Yeast"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/s41596-018-0119-1","article-title":"INFOGEST Static in Vitro Simulation of Gastrointestinal Food Digestion","volume":"14","author":"Brodkorb","year":"2019","journal-title":"Nat. Protoc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A Standardised Static in Vitro Digestion Method Suitable for Food\u2014An International Consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1039\/C9FO01293A","article-title":"A Standardised Semi-Dynamic in Vitro Digestion Method Suitable for Food\u2014An International Consensus","volume":"11","author":"Egger","year":"2020","journal-title":"Food Funct."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.foodhyd.2015.07.025","article-title":"In Vitro Behaviour of Curcumin Nanoemulsions Stabilized by Biopolymer Emulsifiers\u2014Effect of Interfacial Composition","volume":"52","author":"Pinheiro","year":"2016","journal-title":"Food Hydrocoll."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.cofs.2017.06.005","article-title":"Towards the Understanding of the Behavior of Bio-Based Nanostructures during in vitro Digestion","volume":"15","author":"Pinheiro","year":"2017","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1080\/09637486.2020.1763926","article-title":"Influence of the Addition of Different Ingredients on the Bioaccessibility of Glucose Released from Rice during Dynamic in Vitro Gastrointestinal Digestion","volume":"72","author":"Fernandes","year":"2021","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.algal.2017.04.033","article-title":"Flow Cytometry to Estimate the Cell Disruption Yield and Biomass Release of Chlorella Sp. during Bead Milling","volume":"25","author":"Eppink","year":"2017","journal-title":"Algal Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"103158","DOI":"10.1016\/j.algal.2023.103158","article-title":"Evaluation of Efficiency of Disruption Methods for Coelastrella Sp. in Order to Obtain High Yields of Biochemical Compounds Release","volume":"73","author":"Loureiro","year":"2023","journal-title":"Algal Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0308-8146(02)00261-3","article-title":"Protein Contents and Physicochemical Properties in Honey Samples of Apis Mellifera of Different Floral Origins","volume":"80","author":"Azeredo","year":"2003","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.foodchem.2016.02.074","article-title":"Characterization of Coffea Arabica Monofloral Honey from Esp\u00edrito Santo, Brazil","volume":"203","author":"Kadri","year":"2016","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1007\/s13197-019-03708-7","article-title":"Pathway of 5-Hydroxymethyl-2-Furaldehyde Formation in Honey","volume":"56","author":"Yang","year":"2019","journal-title":"J. Food Sci. Technol."},{"key":"ref_39","unstructured":"Minist\u00e9rio da Agricultura e Abastecimento (2000). Regulamento T\u00e9cnico de Identidade e Qualidade Do Mel, Minist\u00e9rio da Agricultura e Abastecimento."},{"key":"ref_40","unstructured":"(2002). European Community (EC) Council Directive 2001\/110\/EC of 20 December 2001 Relating to Honey, European Union."},{"key":"ref_41","unstructured":"(2022). Codex Alimentarius Revised Codex Standard for Honey, United Nations Press."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.foodchem.2004.01.068","article-title":"Characterisation of Spanish Thyme Honeys by Their Physicochemical Characteristics and Mineral Contents","volume":"88","author":"Terrab","year":"2004","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1080\/10942912.2021.1953525","article-title":"Rheological and Thermal Properties of Honey Produced in Algeria and Ethiopia: A Review","volume":"24","author":"Sundramurthy","year":"2021","journal-title":"Int. J. Food Prop."},{"key":"ref_44","first-page":"57","article-title":"Antioxidant Capacity, Physicochemical and Floral Characterization of Honeys from the Northeast of Brazil","volume":"8","author":"Almeida","year":"2016","journal-title":"Rev. Virtual Qu\u00edmica"},{"key":"ref_45","first-page":"450","article-title":"Geographical, Botanical and Chemical Profile of Monofloral Italian Honeys As Food Quality Guarantee and Territory Brand","volume":"151","author":"Manfredini","year":"2016","journal-title":"Plant Biosyst. - Int. J. Deal. Asp. Plant Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/s00284-017-1412-x","article-title":"Quercetin Protects Yeast Saccharomyces Cerevisiae Pep4 Mutant from Oxidative and Apoptotic Stress and Extends Chronological Lifespan","volume":"75","author":"Alugoju","year":"2018","journal-title":"Curr. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Alevia, M., Rasines, S., Cantero, L., Sancho, M.T., Fern\u00e1ndez-Mui\u00f1o, M.A., and Os\u00e9s, S.M. (2021). Chemical Extraction and Gastrointestinal Digestion of Honey: Influence on Its Antioxidant, Antimicrobial and Anti-Inflammatory Activities. Foods, 10.","DOI":"10.3390\/foods10061412"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e38094","DOI":"10.1016\/j.heliyon.2024.e38094","article-title":"Sugar Profile and Sensory Properties of Honey from Different Geographical Zones and Botanical Origins in Tanzania","volume":"10","author":"Mongi","year":"2024","journal-title":"Heliyon"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/S0308-8146(98)00057-0","article-title":"A Review of the Analytical Methods to Determine the Geographical and Botanical Origin of Honey","volume":"63","author":"Anklam","year":"1998","journal-title":"Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"200973745","DOI":"10.33448\/rsd-v9i7.3745","article-title":"Sensorial Quality of Sugarcane Juice with the Addition of Fruits Pulp from the Semi-Arid","volume":"9","author":"Silva","year":"2020","journal-title":"Res. Soc. Dev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"101183","DOI":"10.1016\/j.ijgfs.2025.101183","article-title":"Symbiotic Honey Beverages: A Matrix Which Tells a Story of Survival and Protection of Human Health from a Gastronomic and Industrial Perspective","volume":"40","author":"Shinohara","year":"2025","journal-title":"Int. J. Gastron. Food Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"113717","DOI":"10.1016\/j.foodres.2023.113717","article-title":"Exploring the Potential of Probiotic-Enriched Beer: Microorganisms, Fermentation Strategies, Sensory Attributes, and Health Implications","volume":"175","year":"2024","journal-title":"Food Res. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7588","DOI":"10.1007\/s13197-015-1977-y","article-title":"Flavor Impacts of Glycerol in the Processing of Yeast Fermented Beverages: A Review","volume":"52","author":"Zhao","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.cofs.2020.10.025","article-title":"Plant-Based Milk Substitutes as Emerging Probiotic Carriers","volume":"38","author":"Rasika","year":"2021","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Andrade, R., Santos, E., Azoubel, P., and Ribeiro, E. (2019). Increased Survival of Lactobacillus Rhamnosus ATCC 7469 in Guava Juices with Simulated Gastrointestinal Conditions during Refrigerated Storage. Food Biosci., 32.","DOI":"10.1016\/j.fbio.2019.100470"},{"key":"ref_56","unstructured":"Galanakis, C.M. (2019). Chapter 11\u2014Production and Recovery of Bioaromas Synthesized by Microorganisms. The Role of Alternative and Innovative Food Ingredients and Products in Consumer Wellness, Academic Press."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"106545","DOI":"10.1016\/j.jfca.2024.106545","article-title":"Aroma Active Compounds of Honey: Analysis with GC-MS, GC-O, and Molecular Sensory Techniques","volume":"134","author":"Mahmoud","year":"2024","journal-title":"J. Food Compos. Anal."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1053\/j.gastro.2006.03.023","article-title":"Integrated Upper Gastrointestinal Response to Food Intake","volume":"131","author":"Camilleri","year":"2006","journal-title":"Gastroenterology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.bbagen.2011.03.011","article-title":"Intracellular pH Is a Tightly Controlled Signal in Yeast","volume":"1810","author":"Orij","year":"2011","journal-title":"Biochim. Biophys. Acta BBA - Gen. Subj."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s00253-019-10226-1","article-title":"Microbial Response to Acid Stress: Mechanisms and Applications","volume":"104","author":"Guan","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.1365-2672.2009.04633.x","article-title":"Physiological and Molecular Analysis of the Stress Response of Saccharomyces Cerevisiae Imposed by Strong Inorganic Acid with Implication to Industrial Fermentations","volume":"109","author":"Bonini","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1007\/s10482-015-0568-2","article-title":"Transcriptomic Response of Saccharomyces Cerevisiae for Its Adaptation to Sulphuric Acid-Induced Stress","volume":"108","author":"Elsztein","year":"2015","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/j.1365-2672.2012.05362.x","article-title":"Participation of CWI, HOG and Calcineurin Pathways in the Tolerance of Saccharomyces Cerevisiae to Low pH by Inorganic Acid","volume":"113","author":"Elsztein","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_64","first-page":"423","article-title":"Genetic Interaction between HOG1 and SLT2 Genes in Signalling the Cellular Stress Caused by Sulphuric Acid in Saccharomyces Cerevisiae","volume":"25","author":"Elsztein","year":"2015","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Lucena, R.M., Dolz-Edo, L., Brul, S., de Morais, M.A., and Smits, G. (2020). Extreme Low Cytosolic pH Is a Signal for Cell Survival in Acid Stressed Yeast. Genes, 11.","DOI":"10.3390\/genes11060656"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Alkalbani, N.S., Osaili, T.M., Al-Nabulsi, A.A., Olaimat, A.N., Liu, S.-Q., Shah, N.P., Apostolopoulos, V., and Ayyash, M.M. (2022). Assessment of Yeasts as Potential Probiotics: A Review of Gastrointestinal Tract Conditions and Investigation Methods. J. Fungi, 8.","DOI":"10.3390\/jof8040365"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Pais, P., Almeida, V., Y\u0131lmaz, M., and Teixeira, M.C. (2020). Saccharomyces Boulardii: What Makes It Tick as Successful Probiotic?. J. Fungi, 6.","DOI":"10.3390\/jof6020078"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Aguilar-Ballester, M., Herrero-Cervera, A., Vinu\u00e9, \u00c1., Mart\u00ednez-Herv\u00e1s, S., and Gonz\u00e1lez-Navarro, H. (2020). Impact of Cholesterol Metabolism in Immune Cell Function and Atherosclerosis. Nutrients, 12.","DOI":"10.3390\/nu12072021"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1042\/BJ20112099","article-title":"The Role of the Snf1 Kinase in the Adaptive Response of Saccharomyces Cerevisiae to Alkaline pH Stress","volume":"444","author":"Casamayor","year":"2012","journal-title":"Biochem. J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"4420","DOI":"10.1128\/MCB.01089-14","article-title":"Coregulated Expression of the Na+\/Phosphate Pho89 Transporter and Ena1 Na+-ATPase Allows Their Functional Coupling under High-pH Stress","volume":"34","author":"Canadell","year":"2014","journal-title":"Mol. Cell. Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jbiosc.2017.08.008","article-title":"Analysis of Ambient pH Stress Response Mediated by Iron and Copper Intake in Schizosaccharomyces pombe","volume":"125","author":"Higuchi","year":"2018","journal-title":"J. Biosci. Bioeng."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"D\u017cugan, M., Tomczyk, M., Sowa, P., and Grabek-Lejko, D. (2018). Antioxidant Activity as Biomarker of Honey Variety. Molecules, 23.","DOI":"10.3390\/molecules23082069"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Becerril-S\u00e1nchez, A.L., Quintero-Salazar, B., Dubl\u00e1n-Garc\u00eda, O., and Escalona-Buend\u00eda, H.B. (2021). Phenolic Compounds in Honey and Their Relationship with Antioxidant Activity, Botanical Origin, and Color. Antioxidants, 10.","DOI":"10.3390\/antiox10111700"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Schell, K.R., Fernandes, K.E., Shanahan, E., Wilson, I., Blair, S.E., Carter, D.A., and Cokcetin, N.N. (2022). The Potential of Honey as a Prebiotic Food to Re-Engineer the Gut Microbiome Toward a Healthy State. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.957932"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Dimitriu, L., Constantinescu-Aruxandei, D., Preda, D., Moraru, I., B\u0103beanu, N.E., and Oancea, F. (2023). The Antioxidant and Prebiotic Activities of Mixtures Honey\/Biomimetic NaDES and Polyphenols Show Differences between Honeysuckle and Raspberry Extracts. Antioxidants, 12.","DOI":"10.3390\/antiox12091678"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Agarbati, A., Canonico, L., Marini, E., Zannini, E., Ciani, M., and Comitini, F. (2020). Potential Probiotic Yeasts Sourced from Natural Environmental and Spontaneous Processed Foods. Foods, 9.","DOI":"10.3390\/foods9030287"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s10295-005-0027-6","article-title":"Isolation by Genetic and Physiological Characteristics of a Fuel-Ethanol Fermentative Saccharomyces Cerevisiae Strain with Potential for Genetic Manipulation","volume":"32","author":"Antunes","year":"2005","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1111\/jam.13666","article-title":"Flow Cytometry: A Versatile Technology for Specific Quantification and Viability Assessment of Micro-Organisms in Multistrain Probiotic Products","volume":"124","author":"Chiron","year":"2018","journal-title":"J. Appl. Microbiol."}],"container-title":["Fermentation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2311-5637\/11\/7\/405\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:09:48Z","timestamp":1760033388000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2311-5637\/11\/7\/405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,15]]},"references-count":78,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["fermentation11070405"],"URL":"https:\/\/doi.org\/10.3390\/fermentation11070405","relation":{},"ISSN":["2311-5637"],"issn-type":[{"type":"electronic","value":"2311-5637"}],"subject":[],"published":{"date-parts":[[2025,7,15]]}}}