{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:33:17Z","timestamp":1761744797602,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T00:00:00Z","timestamp":1532390400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/87758\/2012","FCT UID\/CTM\/ 50011\/2013","PEst-C\/EQB\/LA0006\/2013"],"award-info":[{"award-number":["SFRH\/BD\/87758\/2012","FCT UID\/CTM\/ 50011\/2013","PEst-C\/EQB\/LA0006\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fermentation"],"abstract":"<jats:p>The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 \u00b1 2.7%), Alphaproteobacteria (13.6 \u00b1 1.3%) and Gammaproteobacteria (2.40 \u00b1 1.1%) and Bacteroidetes (9.20 \u00b1 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.<\/jats:p>","DOI":"10.3390\/fermentation4030058","type":"journal-article","created":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T11:51:38Z","timestamp":1532433098000},"page":"58","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Impact of Organic Acids Supplementation to Hardwood Spent Sulfite Liquor as Substrate for the Selection of Polyhydroxyalkanoates-Producing Organisms"],"prefix":"10.3390","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9972-496X","authenticated-orcid":false,"given":"Diogo","family":"Queir\u00f3s","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica\/CICECO, Universidade de Aveiro, Campus Universit\u00e1rio de Santiago, P-3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0550-2723","authenticated-orcid":false,"given":"Catarina","family":"Rangel","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica\/CICECO, Universidade de Aveiro, Campus Universit\u00e1rio de Santiago, P-3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6094-0107","authenticated-orcid":false,"given":"Paulo","family":"Costa Lemos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Associado para a Qu\u00edmica Verde, Rede de Qu\u00edmica e Tecnologia (LAQV, REQUIMTE), Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, P-2829-516 Caparica, Portugal"}]},{"given":"Simona","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Water Research Institute-Consiglio Nazionale della Ricerche (CNR), Via Salaria Km 29,300, 00015 Monterotondo, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5802-1777","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Seuanes Serafim","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica\/CICECO, Universidade de Aveiro, Campus Universit\u00e1rio de Santiago, P-3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,7,24]]},"reference":[{"key":"ref_1","unstructured":"(2017, July 21). 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