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Yeasts of the genus Rhodotorula can biosynthesize large amounts of carotenoids under environmental stress conditions, and this process may become industrially feasible if production costs are minimized using low-cost by-products as nutrient sources. As cassava roots are produced and consumed in large quantities in the Amazon biome, our research aimed to investigate the best cultivation conditions in a benchtop bioreactor for the biotechnological production of carotenoids by R. glutinis using manipueira as a low-cost substrate. Manipueira is wastewater generated during cassava flour production, and it was concentrated at 12 \u00b0Brix for this study. In addition, the carotenoid composition, at the best cultivation condition, was analyzed by LC-MS. Higher carotenoid contents (1410 \u03bcg\/g of dried biomass) than described in the literature, and biomass (10 g), were produced by R. glutinis using concentrated manipueira as a low-cost substrate after cultivation at pH 5, 35 \u00b0C and agitation at 150 rpm. At the best cultivation conditions, \u03b2-carotene, lutein, and 5,8-epoxy-lutein were the major carotenoids, with the latter two compounds being identified for the first time in the biomass of R. glutinis. Therefore, concentrated manipueira can be seen as a feasible alternative and low-cost substrate to be used for the biotechnological production of high carotenoid contents by R. glutinis.<\/jats:p>","DOI":"10.3390\/fermentation9070617","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T01:43:13Z","timestamp":1688002993000},"page":"617","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Concentrated Manipueira as an Alternative Low-Cost Substrate to Rhodotorula glutinis for Biotechnological Production of High Contents of Carotenoids"],"prefix":"10.3390","volume":"9","author":[{"given":"Felipe de Andrade","family":"Maia","sequence":"first","affiliation":[{"name":"Faculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, Brazil"}]},{"given":"Willen Silva","family":"Igreja","sequence":"additional","affiliation":[{"name":"Postgraduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3770-1658","authenticated-orcid":false,"given":"Ana Augusta Odorissi","family":"Xavier","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas (UNICAMP), S\u00e3o Paulo 13083-862, Brazil"}]},{"given":"Adriana Zerlotti","family":"Mercadante","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas (UNICAMP), S\u00e3o Paulo 13083-862, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8584-5859","authenticated-orcid":false,"given":"Alessandra Santos","family":"Lopes","sequence":"additional","affiliation":[{"name":"Faculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, Brazil"},{"name":"Postgraduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4549-3297","authenticated-orcid":false,"given":"Renan Campos","family":"Chist\u00e9","sequence":"additional","affiliation":[{"name":"Faculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, Brazil"},{"name":"Postgraduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1590\/S1516-8913201400152","article-title":"Torularhodin and torulene: Bioproduction, properties and prospective applications in food and cosmetics\u2014A review","volume":"58","author":"Zoz","year":"2015","journal-title":"Braz. 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