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Oleaginous yeasts are a potential renewable source of biofuels. However, the yeast-derived biofuels cost is still non-competitive with the fossil fuel prices. To improve the sustainability of yeast-derived biofuels, it is necessary to valorize all yeast biomass fractions, an approach based on the biorefinery concept. This review describes the present situation of the oleaginous yeast biorefinery research, emphasizing the feedstock, processes and techniques involved in this concept, as well as on potential bioproducts that can be obtained from oleaginous yeast biomass.<\/jats:p>","DOI":"10.3390\/fermentation9121013","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T05:23:22Z","timestamp":1702358602000},"page":"1013","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Oleaginous Yeast Biorefinery: Feedstocks, Processes, Techniques, Bioproducts"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4365-8868","authenticated-orcid":false,"given":"Teresa","family":"Lopes da Silva","sequence":"first","affiliation":[{"name":"Unidade de Bioenergia e Biorrefinarias, Laborat\u00f3rio Nacional de Energia e Geologia, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]},{"given":"Afonso","family":"Fontes","sequence":"additional","affiliation":[{"name":"Unidade de Bioenergia e Biorrefinarias, Laborat\u00f3rio Nacional de Energia e Geologia, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1405-9981","authenticated-orcid":false,"given":"Alberto","family":"Reis","sequence":"additional","affiliation":[{"name":"Unidade de Bioenergia e Biorrefinarias, Laborat\u00f3rio Nacional de Energia e Geologia, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1439-9214","authenticated-orcid":false,"given":"Carla","family":"Siva","sequence":"additional","affiliation":[{"name":"Instituto Dom Luiz (IDL), Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, 1747-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5376-8430","authenticated-orcid":false,"given":"Francisco","family":"G\u00edrio","sequence":"additional","affiliation":[{"name":"Unidade de Bioenergia e Biorrefinarias, Laborat\u00f3rio Nacional de Energia e Geologia, Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,12]]},"reference":[{"key":"ref_1","unstructured":"(2023, September 09). IEA Bioenergy Task42. Available online: https:\/\/www.ieabioenergy.com\/wp-content\/uploads\/2014\/09\/IEA-Bioenergy-Task42-Biorefining-Brochure-SEP2014_LR.pdf."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Jacob-Lopes, E., and Zepka, L. (2015). Biomass Production and Uses, IntechOpen. Chapter 5.","DOI":"10.5772\/58664"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.biortech.2017.01.006","article-title":"Microalgae biorefinery: High value products perspectives","volume":"229","author":"Chew","year":"2017","journal-title":"Bioresour. Techol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sivaramakrishnan, R., Suresh, S., Kanwal, S., Ramadoss, G., Ramprakash, B., and Incharoensakdi, A. (2022). Microalgal biorefinery concepts\u2019 development for biofuel and bioproducts: Current perspective and bottlenecks. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23052623"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Olgu\u00edn, E.J., S\u00e1nchez-Galv\u00e1n, G., Arias-Olgu\u00edn, I.I., Melo, F.J., Gonz\u00e1lez-Portela, R.E., Cruz, L., De Philippis, R., and Adessi, A. (2022). Microalgae-Based Biorefineries: Challenges and Future Trends to Produce Carbohydrate Enriched Biomass, High-Added Value Products and Bioactive Compounds. Biology, 11.","DOI":"10.3390\/biology11081146"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"102698","DOI":"10.1016\/j.algal.2022.102698","article-title":"Heterotrophic vs autotrophic production of microalgae: Bringing some light into everlasting cost controversy","volume":"64","author":"Ruiz","year":"2022","journal-title":"Algal Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1007\/s00253-011-3200-z","article-title":"Oily yeasts as oleaginous cell factories","volume":"90","author":"Ageitos","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Caporusso, A., Capece, A., and De Bari, I. (2021). Oleaginous Yeasts as Cell Factories for the Sustainable Production of Microbial Lipids by the Valorization of Agri-Food Wastes. Fermentation, 7.","DOI":"10.3390\/fermentation7020050"},{"key":"ref_9","unstructured":"Morata, A., and Loira, I. (2017). Yeast, IntechOpen. Chapter 1."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"129112","DOI":"10.1016\/j.jclepro.2021.129112","article-title":"Biodiesel production of Rhodosporidium toruloides using different carbon sources of sugar-containing wastewater: Experimental analysis and model verification","volume":"323","author":"Liu","year":"2021","journal-title":"J. Clean Prod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.bej.2017.08.013","article-title":"Yarrowia lipolytica as a workhorse for biofuel production","volume":"127","author":"Darvishi","year":"2017","journal-title":"Biochem. Eng. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"12501","DOI":"10.1021\/acs.energyfuels.8b02231","article-title":"Lipid production by the yeast Trichosporon oleaginosus on the enzymatic hydrolysate of alkaline pretreated corn cobs for biodiesel production","volume":"32","author":"Santek","year":"2018","journal-title":"Energy Fuels"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1002\/bit.26337","article-title":"Oleaginous yeasts: Promising platforms for the production of oleochemicals and biofuels","volume":"114","author":"Adrio","year":"2017","journal-title":"Biotechnol. Bioeng."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Poontawee, R., Lorliam, W., Polburee, P., and Limtong, S. (2023). Oleaginous yeasts: Biodiversity and cultivation. Fungal Biol. Rev., 44.","DOI":"10.1016\/j.fbr.2022.11.003"},{"key":"ref_15","first-page":"280","article-title":"A Review on oleaginous microorganisms for biological wastewater treatment: Current and future prospect","volume":"9","author":"Rayaan","year":"2021","journal-title":"J. Environ. Treat. Tech."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1007\/s12010-016-2386-z","article-title":"Converting chemical oxygen demand (COD) of cellulosic ethanol fermentation wastewater into microbial lipid by oleaginous yeast Trichosporon cutaneum","volume":"182","author":"Wang","year":"2017","journal-title":"Appl. Biochem. Biotecnhol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.renene.2012.12.017","article-title":"Microbial conversion of wastewater from butanol fermentation to microbial oil by oleaginous yeast Trichosporon dermatis","volume":"55","author":"Peng","year":"2013","journal-title":"Renew. Energy"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.energy.2012.12.026","article-title":"Lipid and carotenoid production by oleaginous yeast Rhodotorula glutinis cultivated on brewery effluents","volume":"61","author":"Schneider","year":"2013","journal-title":"Energy"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.2166\/wst.2013.059","article-title":"Research on microbial lipid production from potato starch wastewater as culture medium by Lipomyces starkeyi","volume":"67","author":"Liu","year":"2013","journal-title":"Water Sci. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.procbio.2020.03.015","article-title":"Concomitant wastewater treatment with lipid and carotenoid production by the oleaginous yeast Rhodosporidium toruloides grown on brewery effluent enriched with sugarcane molasses and urea","volume":"94","author":"Dias","year":"2020","journal-title":"Process Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"128860","DOI":"10.1016\/j.foodchem.2020.128860","article-title":"Microbial production of value-added products and enzymes from molasses, a by-product of sugar industry","volume":"346","author":"Zhang","year":"2021","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"101281","DOI":"10.1016\/j.eti.2020.101281","article-title":"Valorization of molasses by oleaginous yeasts for single cell oil (SCO) and carotenoids production","volume":"21","author":"Lakshmidevi","year":"2022","journal-title":"Env. Technol. Innov."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1002\/jctb.5985","article-title":"Valorization of sugarcane molasses for the production of microbial lipids via fermentation of two Rhodosporidium strains for enzymatic synthesis of polyol esters","volume":"95","author":"Boviatsi","year":"2020","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jbiotec.2019.02.012","article-title":"Biomass and lipid production by Rhodococcus opacus PD630 in molasses based media with and without osmotic-stress","volume":"297","author":"Saisriyoot","year":"2019","journal-title":"J. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Donzella, S., Serra, I., Fumagalli, A., Pellegrino, L., Mosconi, G., Scalzo, R., and Compagno, C. (2022). Recycling industrial food wastes for lipid production by oleaginous yeasts Rhodosporidiobolus azoricus and Cutaneotrichosporon oleaginosum. Biotechnol. Biofuels Bioprod., 15.","DOI":"10.1186\/s13068-022-02149-3"},{"key":"ref_26","first-page":"211","article-title":"Optimization of lipid production in the oleaginous yeast Apiotrichum curvatum in wheypermeate","volume":"29","author":"Ykema","year":"1988","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Costa, A.R., Fernandes, H., Salgado, J.M., and Belo, I. (2023). Solid state and semi-solid fermentations of olive and sunflower cakes with Yarrowia lipolytica: Impact of Biological and Physical Pretreatments. Fermentation, 9.","DOI":"10.3390\/fermentation9080734"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5190","DOI":"10.1016\/j.biortech.2010.01.146","article-title":"Ethanol production from carob extract by using Saccharomyces cerevisae","volume":"101","author":"Turhan","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_29","first-page":"S64","article-title":"Evaluation of carop pulp as fermentation substrate for biohydrogen and organic acids production: Sugars richness versus toxicity potential","volume":"11","author":"Lima","year":"2012","journal-title":"Environ. Eng. Manag. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.biteb.2018.07.008","article-title":"Carob pulp syrup: A potential Mediterranean carbon source for carotenoids production by Rhodosporidium toruloides NCYC 921","volume":"3","author":"Martins","year":"2018","journal-title":"Bioresour. Technol. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Francisco, M., Aguiar, T.Q., Abreu, G., Marques, S., G\u00edrio, F., and Domingues, L. (2023). Single-cell oil production by engineered Ashbya gossypii from non-detoxified lignocellulosic biomass hydrolysate. Fermentation, 9.","DOI":"10.3390\/fermentation9090791"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1186\/s12934-021-01710-3","article-title":"Oleaginous yeasts-substrate preference and lipid productivity: A view on the performance of microbial lipid producers","volume":"20","author":"Shaigani","year":"2021","journal-title":"Microbial Cell Fact."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Brandenburg, J., Blomqvist, J., Shapaval, V., Kohler, A., Samples, S., Sandgen, M., and Passoth, V. (2021). Oleaginous yeasts respond differently to carbon source present in lignocellulose hydrolysate. Biotechnol. Biofuels, 14.","DOI":"10.1186\/s13068-021-01974-2"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kumar, L., Yellapu, S., Tyagi, S., Tyagi, R., and Zhang, X. (2019). A review on variation in crude glycerol composition, bio-valorization of crude and purified glycerol as carbon source for lipid production. Bioresour. Technol., 293.","DOI":"10.1016\/j.biortech.2019.122155"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kosamia, N., Samavi, M., Uprety, B., and Rakshit, S. (2020). Valorisation of biodiesel byproduct crude glycerol for the production of bioenergy and biochemicals. Catalysts, 10.","DOI":"10.3390\/catal10060609"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1016\/j.biortech.2017.05.163","article-title":"From low-cost substrates to single cell oils synthesized by oleaginous yeasts","volume":"245","author":"Qin","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s12934-016-0467-x","article-title":"Assessing an affective feeding strategy to optimize crude glycerol utilization as sustainable carbon source for lipid accumulation in oleaginous yeasts","volume":"15","author":"Signori","year":"2016","journal-title":"Microbial Cell Fact."},{"key":"ref_38","first-page":"923","article-title":"Performance evaluation of a yeast biorefinery as a sustainable model for co-production of biomass, bioemulsifier, lipid, biodiesel and animal-feed components using inexpensive raw materials. Sustain","volume":"1","author":"Kumar","year":"2017","journal-title":"Energy Fuels"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.funbio.2015.09.002","article-title":"Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol","volume":"119","author":"Polburee","year":"2015","journal-title":"Fungal Biol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Llamas, M., Dourou, M., Gonz\u00e1lez-Fern\u00e1ndez, C., Aggelis, G., and Tom\u00e1s-Rego, E. (2020). Screening of oleaginous yeasts for lipid production using fatty acids as substrate. Biomass Bioenergy, 138.","DOI":"10.1016\/j.biombioe.2020.105553"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1007\/s12257-010-0370-y","article-title":"Exproring low-cost carbon sources for microbial lipids production by fed-batch cultivation of Cryptococcus albidus","volume":"15","author":"Fei","year":"2011","journal-title":"Biotechnol. Bioprocess Eng."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Gao, R., Zhou, X., Bao, W., Cheng, S., and Zheng, L. (2020). Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions. Biotechnol. Biofuels, 13.","DOI":"10.1186\/s13068-019-1645-y"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1089\/omi.2010.0072","article-title":"Adaptive response and tolerance to weak acids in Saccharomyces cerevisae. A genome-wide view","volume":"14","author":"Mira","year":"2010","journal-title":"Omics A J. Int. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"116292","DOI":"10.1016\/j.fuel.2019.116292","article-title":"Lipid recovery from oleaginous yeasts: Perspectives and challenges for industrial applications","volume":"259","author":"Khot","year":"2020","journal-title":"Fuel"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jbiotec.2014.10.004","article-title":"Enhanced lipid accumulation in the yeast Yarrowia lipolytica by over-expression of ATP:citrate lyase from Mus musculus","volume":"192 PtA","author":"Zhang","year":"2014","journal-title":"J. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.biochi.2004.09.017","article-title":"Fatty acid biosynthesis in microorganisms being used for single cell oil production","volume":"86","author":"Ratledge","year":"2004","journal-title":"Biochemie"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"113043","DOI":"10.1016\/j.rser.2022.113043","article-title":"Factors affecting the optimization and scale-up of lipid accumulation in oleaginous yeasts for sustainable biofuels production","volume":"171","author":"Veiga","year":"2023","journal-title":"Renew Sust. Energ. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Mota, M., M\u00fagica, P., and S\u00e1-Correia, I. (2022). Exploring yeast diversity to produce lipid-based biofuels from agro-forestry and industrial organic residues. J. Fungi, 8.","DOI":"10.3390\/jof8070687"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1002\/ejlt.201100014","article-title":"Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production","volume":"113","author":"Papanikolaou","year":"2011","journal-title":"Eur. J. Lipid. Sci. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Karamerou, E., and Webb, C. (2019). Cultivation modes for microbial oil production using oleaginous yeasts\u2014A review. Biochem. Eng. J., 151.","DOI":"10.1016\/j.bej.2019.107322"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Cohen, Z., and Ratledge, C. (2005). Single Cell Oils, AOCS Press. Chapter 6.","DOI":"10.4324\/9781003040101"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"119811","DOI":"10.1016\/j.fuel.2020.119811","article-title":"Bioconversion of volatile fatty acids into lipids by oleaginous yeast Apiotrichum porosum DSM27194","volume":"290","author":"Qian","year":"2021","journal-title":"Fuel"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.bej.2016.04.024","article-title":"Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density","volume":"112","author":"Polburee","year":"2016","journal-title":"Biochem. Eng. J."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Polburee, P., and Limtong, S. (2020). Economical lipid production from crude glycerol using Rhodosporidiobolus fluvialis DMKU-RK253 in a two-stage cultivation under non-sterile conditions. Biomass Bioenergy, 138.","DOI":"10.1016\/j.biombioe.2020.105597"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.biortech.2014.01.039","article-title":"Lipid and carotenoid production by Rhodotorula glutinis under irradiation\/high-temperature and dark\/low-temperature cultivation","volume":"157","author":"Zhang","year":"2014","journal-title":"Bioresour. Technol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Grubisic, M., Mihajlovki, K., Gruicic, A.M., Beluham, S., Santek, B., and Ivanci\u00e9, S. (2021). Strategies for improvement of lipid production by yeast Trichosporon oleaginosus form lignocellulosic biomass. J. Fungi, 7.","DOI":"10.3390\/jof7110934"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.sjbs.2012.04.005","article-title":"Microalgae and wastewater treatment","volume":"19","author":"Ibraheem","year":"2012","journal-title":"Saudi J Biol. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.procbio.2021.12.019","article-title":"Primary brewery wastewater as feedstock for the yeast Rhodosporidium toruloides and the microalga Tetradesmus obliquus mixed cultures with lipid production","volume":"113","author":"Dias","year":"2021","journal-title":"Proc. Biochem."},{"key":"ref_59","first-page":"554","article-title":"An industrial application of multiparameter flow cytometry: Assessment of cell physiological state and its application to the study of microbial fermentations","volume":"59","author":"Hewitt","year":"2001","journal-title":"Cytometry"},{"key":"ref_60","unstructured":"(2023, November 08). Available online: https:\/\/eu.sysmex-flowcytometry.com\/reagents\/yeastcontrol\/2935\/yeast-control-neutral-lipids#."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Chen, J., Wei, D., and Pohnert, G. (2017). Rapid estimation of astaxantin and the carotenoid-to-chlorophyll ratio in the green microalga Chromochloris zofingiensis using flow cytometry. Mar. Drugs, 15.","DOI":"10.3390\/md15070231"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Ami, D., Posteri, R., Mereghetti, P., Porro, D., Doglia, S., and Branduardi, P. (2014). Fourier transform infrared spectroscopy as a method to study lipid accumulation in oleaginous yeasts. Biotechnol. Biofuels, 7.","DOI":"10.1186\/1754-6834-7-12"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1080\/00194506.2020.1847699","article-title":"Online measurements of dissolved oxygen in shake flask to elucidate its role on caffeine degradation by Pseudomonas sp.","volume":"64","author":"Shanmugam","year":"2022","journal-title":"Indian Chem. Eng."},{"key":"ref_64","first-page":"102621","article-title":"Recent developments in oleaginous yeast feedstock based biorefinery for production and life cycle assessment of biofuels and value-added products. Sustain","volume":"53","author":"Chopra","year":"2022","journal-title":"Energy Technol. Assess."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Zainuddin, M., Fai, C., Ariff, A., Rios-Solis, L., and Halim, M. (2021). Current pretreatment\/Cell disruption and extraction methods used to improve intracellular lipid recovery from oleaginous yeasts. Microorganisms, 9.","DOI":"10.3390\/microorganisms9020251"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.ifset.2016.06.017","article-title":"Yeast cell disruption strategies for recovery of intracellular bio-active compounds\u2014A review","volume":"36","author":"Liu","year":"2016","journal-title":"Inn. Food Sci. Emerg. Technol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Saini, R.K., Prasad, P., Sahng, X., and Keum, Y. (2021). Lipid extraction methods. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222413643"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Kapoore, R., Butler, T.O., Pandhal, J., and Vaidyanathan, S. (2018). Microwave-assisted extraction for microalgae: From biofuels to biorefinery. Biology, 7.","DOI":"10.3390\/biology7010018"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"107868","DOI":"10.1016\/j.cep.2020.107868","article-title":"Current insight in yeast cell disruption technologies for cell recovery. A review","volume":"150","author":"Koubaa","year":"2020","journal-title":"Chem. Eng. Process. Process Intensif."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Breil, C., Vian, M., Zemb, T., Kunz, W., and Chemat, F. (2017). Bligh and Dyer and Folch methods for solid-liquid-liquid extractions of lipids from microorganisms. Comprehension of solvatation mechanisms and toward substitution with alternative solvents. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18040708"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Gorte, O., Hollenbach, R., Papachristou, I., Steinweg, C., Silve, A., Frey, W., Syldatk, C., and Ochsenteither, K. (2020). Evaluation of downstream processing, extraction and quantification strategies for single cell oil produced by the oleaginous yeast Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194. Front. Bioeng. Biotechol., 8.","DOI":"10.3389\/fbioe.2020.00355"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.indcrop.2017.07.036","article-title":"Economic feasibility analysis of soybean oil production by hexane extraction","volume":"108","author":"Cheng","year":"2017","journal-title":"Ind. Crops Prod."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1002\/jctb.3840","article-title":"Extraction of lipids from Yarrowia lipolytica","volume":"88","author":"Milanesio","year":"2013","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ultsonch.2018.08.003","article-title":"Enhanced lipid extraction from oleaginous yeast biomass using ultrasound assisted extraction: A greener and scalable process","volume":"52","author":"Kumar","year":"2019","journal-title":"Ultrasonics Sonochem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"490","DOI":"10.3390\/encyclopedia3020034","article-title":"Oleaginous red yeasts: Concomitant producers of triacylglycerols and carotenoids","volume":"3","author":"Fakankun","year":"2023","journal-title":"Encyclopedia"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1186\/s13568-018-0658-4","article-title":"Optimization of lipid extraction from the oleaginous yeasts Rhodotorula glutinis and Lipomyces kenonenkoae","volume":"8","author":"Vasconcelos","year":"2018","journal-title":"AMB Express"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1007\/s12649-018-0489-1","article-title":"Sequential carotenoids extraction and biodiesel production from Rhodosporidium toruloides","volume":"11","author":"Passarinho","year":"2020","journal-title":"Waste Biomass Val."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.biortech.2016.11.113","article-title":"Wastes valorization from Rhodosporidium toruloides NCYC 921 production and biorefinery by anaerobic digestion","volume":"226","author":"Batista","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Banerjee, A., Bansal, N., Kumar, J., Bhaskar, T., Ray, A., and Ghosh, D. (2021). Characterization of the de-oiled yeast biomass for plausible value mapping in a biorefinery perspective. Bioresour. Technol., 337.","DOI":"10.1016\/j.biortech.2021.125422"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.apenergy.2017.11.031","article-title":"Generating biocrude form partially defatted Cryptococcus curvatus yeast residues through catalytic liquefaction","volume":"209","author":"Bi","year":"2018","journal-title":"Appl. Energy"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Vysoka, M., Szotkowski, M., Slaninova, E., Dzuricka, L., Strecanska, P., Blazkova, J., and Marova, I. (2023). Oleaginous yeast extracts and their possible effects on human health. Microorganisms, 11.","DOI":"10.3390\/microorganisms11020492"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Parsons, S., Allen, M.J., and Chuck, C.J. (2020). Coproducts of algae and yeast-derived single cell oils: A critical review of their role in improving biorefinery sustainability. Bioresour. Technol., 303.","DOI":"10.1016\/j.biortech.2020.122862"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"118967","DOI":"10.1016\/j.fuel.2020.118967","article-title":"Prediction of environmental impacts of yeast biodiesel production from cardoon stalks at industrial scale","volume":"283","author":"Barbanera","year":"2021","journal-title":"Fuel"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"122349","DOI":"10.1016\/j.jclepro.2020.122349","article-title":"Environmental impact analysis of oleaginous yeast based biodiesel and biocrude production by life cycle assessment","volume":"271","author":"Chopra","year":"2020","journal-title":"J. Clean. Prod."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"121559","DOI":"10.1016\/j.fuel.2021.121559","article-title":"An application of the life cycle thinking: Green refinery enhancements","volume":"305","author":"Caretta","year":"2021","journal-title":"Fuel"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1039\/C9SE00540D","article-title":"Yeast lipid-based biofuels and oleochemicals from lignocellulosic biomass: Life cycle impact assessment","volume":"4","author":"Sharma","year":"2020","journal-title":"Sust. Energy Fuels"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1016\/j.jclepro.2019.05.315","article-title":"Sustainability and life cycle assessment (LCA) of macroalgae-derived single cell oils","volume":"232","author":"Parsons","year":"2019","journal-title":"J. Clean. Prod."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1002\/jctb.5811","article-title":"Techno-economic analysis (TEA) of microbial oil production from wastes resources as part of a biorefinery concept: Assessment at multiple scales under uncertainty","volume":"94","author":"Parsons","year":"2018","journal-title":"Chem. Technol. Biotechnol."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Jena, U., McCurdy, A.T., Warren, A., Summers, H., Ledbetter, R., Hoekman, S., Seefeldt, L., and Quinn, J. (2015). Oleaginous yeast platform for producing biofuels via co-solvent hydrothermal liquefaction. Biotechnol. Biofuels, 8.","DOI":"10.1186\/s13068-015-0345-5"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"3196","DOI":"10.1021\/acssuschemeng.6b00243","article-title":"The techno-economic basis for coproduct manufacturing to enable hydrocarbon fuel production from lignocellulosic biomass","volume":"4","author":"Biddy","year":"2016","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Karamerou, E., Parsons, S., McManus, M., and Chuck, C. (2021). Using techno-economic modelling to determine the minimum cost possible for a microbial palm oil substitute. Biotechnol. 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