{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T01:49:20Z","timestamp":1771033760928,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T00:00:00Z","timestamp":1609891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04469\/2020"],"award-info":[{"award-number":["UIDB\/04469\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000004"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000004"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fibers"],"abstract":"<jats:p>Fabric structures are prone to contamination with microorganisms, as their morphology and ability to retain moisture creates a proper environment for their growth. In this work, a novel, easily processed and cheap coating for a nylon fabric with antimicrobial characteristics was developed. After plasma treatment, made to render the fabric surface more reactive sites, the fabric was impregnated with chitosan and silver nanoparticles by simply dipping it into a mixture of different concentrations of both components. Silver nanoparticles were previously synthesized using the Lee\u2013Meisel method, and their successful obtention was proven by UV\u2013Vis, showing the presence of the surface plasmon resonance band at 410 nm. Nanoparticles with 25 nm average diameter observed by STEM were stable, mainly in the presence of chitosan, which acted as a surfactant for silver nanoparticles, avoiding their aggregation. The impregnated fabric possessed bactericidal activity higher for Gram-positive Staphylococcus aureus than for Gram-negative Pseudomonas aeruginosa bacteria for all combinations. The percentage of live S. aureus and P. aeruginosa CFU was reduced to less than 20% and 60%, respectively, when exposed to each of the coating combinations. The effect was more pronounced when both chitosan and silver were present in the coating, suggesting an effective synergy between these components. After a washing process, the antimicrobial effect was highly reduced, suggesting that the coating is unstable after washing, being almost completely removed from the fabric. Nevertheless, the new-coated fabric can be successfully used in single-use face masks. To our knowledge, the coating of nylon fabrics intended for face-mask material with both agents has never been reported.<\/jats:p>","DOI":"10.3390\/fib9010003","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T09:15:16Z","timestamp":1609924516000},"page":"3","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["New Textile for Personal Protective Equipment\u2014Plasma Chitosan\/Silver Nanoparticles Nylon Fabric"],"prefix":"10.3390","volume":"9","author":[{"given":"Cl\u00e1udia M.","family":"Botelho","sequence":"first","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Margarida M.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, Universidade do Minho, 4710-057 Braga, Portugal"},{"name":"Centro\/Departamento de F\u00edsica, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Jefferson M.","family":"Souza","sequence":"additional","affiliation":[{"name":"CBMDE, Design and Styling, Federal University of Piau\u00ed, Teresina, PI 64049-550, Brazil"}]},{"given":"Nicolina","family":"Dias","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Ana M.","family":"Sousa","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4918-3704","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3303-6563","authenticated-orcid":false,"given":"Raul","family":"Fangueiro","sequence":"additional","affiliation":[{"name":"2C2T\u2014Centro de Ci\u00eancia e Tecnologia T\u00eaxtil, Universidade do Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5299-4164","authenticated-orcid":false,"given":"Andrea","family":"Zille","sequence":"additional","affiliation":[{"name":"2C2T\u2014Centro de Ci\u00eancia e Tecnologia T\u00eaxtil, Universidade do Minho, 4800-058 Guimar\u00e3es, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,6]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Toxicity of Nanoparticles and an Overview of Current Experimental Models","volume":"20","author":"Bahadar","year":"2016","journal-title":"Iran. Biomed. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1177\/0022034517709739","article-title":"Developing a New Generation of Antimicrobial and Bioactive Dental Resins","volume":"96","author":"Cheng","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Cinteza, L.O., Scomoroscenco, C., Voicu, S.N., Nistor, C.L., Nitu, S.G., Trica, B., Jecu, M.-L., and Petcu, C. (2018). Chitosan-Stabilized Ag Nanoparticles with Superior Biocompatibility and Their Synergistic Antibacterial Effect in Mixtures with Essential Oils. Nanomaterials, 8.","DOI":"10.3390\/nano8100826"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"52398","DOI":"10.1039\/C7RA08359A","article-title":"Development of noncytotoxic silver\u2013chitosan nanocomposites for efficient control of biofilm forming microbes","volume":"7","author":"Sebastian","year":"2017","journal-title":"RSC Adv."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1477-3155-3-6","article-title":"Interaction of silver nanoparticles with HIV-1","volume":"3","author":"Elechiguerra","year":"2005","journal-title":"J. Nanobiotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Sun, R.W., Chen, R., Chung, N.P., Ho, C.M., Lin, C.L., and Che, C.M. (2005). Silver nanoparticles fabricated in Hepes buffer exhibit cytoprotective activities toward HIV-1 infected cells. Chem. Commun., 5059\u20135061.","DOI":"10.1039\/b510984a"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1177\/135965350801300210","article-title":"Silver nanoparticles inhibit hepatitis B virus replication","volume":"13","author":"Lu","year":"2008","journal-title":"Antivir. Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1021\/bc900215b","article-title":"Inhibition of Herpes Simplex Virus Type 1 Infection by Silver Nanoparticles Capped with Mercaptoethane Sulfonate","volume":"20","author":"Shukla","year":"2009","journal-title":"Bioconjug. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1166\/jbn.2008.012","article-title":"Silver Nanoparticles Inhibit Replication of Respiratory Syncytial Virus","volume":"4","author":"Sun","year":"2008","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s11671-008-9128-2","article-title":"A Preliminary Assessment of Silver Nanoparticle Inhibition of Monkeypox Virus Plaque Formation","volume":"3","author":"Rogers","year":"2008","journal-title":"Nanoscale Res. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.jviromet.2011.09.003","article-title":"Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro","volume":"178","author":"Xiang","year":"2011","journal-title":"J. Virol. Methods"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zachar, O. (2020). Formulations for COVID-19 Treatment via Silver Nanoparticles Inhalation Delivery at Home and Hospital. ScienceOpen Preprints, 1.","DOI":"10.14293\/S2199-1006.1.SOR-.PPHBJEO.v1"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.apsusc.2014.12.153","article-title":"Hybrid biocomposite with a tunable antibacterial activity and bioactivity based on RF magnetron sputter deposited coating and silver nanoparticles","volume":"329","author":"Ivanova","year":"2015","journal-title":"Appl. Surf. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Alissawi, N., Peter, T., Strunskus, T., Ebbert, C., Grundmeier, G., and Faupel, F. (2013). Plasma-polymerized HMDSO coatings to adjust the silver ion release properties of Ag\/polymer nanocomposites. J. Nanopart. Res., 15.","DOI":"10.1007\/s11051-013-2080-9"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"020801","DOI":"10.1116\/1.3559547","article-title":"Status and potential of atmospheric plasma processing of materials","volume":"29","author":"Pappas","year":"2011","journal-title":"J. Vac. Sci. Technol. A"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4165","DOI":"10.1016\/j.apsusc.2010.11.190","article-title":"Surface treatment of aramid fiber by air dielectric barrier discharge plasma at atmospheric pressure","volume":"257","author":"Jia","year":"2011","journal-title":"Applied Surface Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5006","DOI":"10.1016\/j.surfcoat.2012.06.020","article-title":"Deposition of silver ions onto DBD and DCSBD plasma treated nonwoven polypropylene","volume":"206","author":"Kuraica","year":"2012","journal-title":"Surf. Coat. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/ppap.201400052","article-title":"Plasma Treatment in Textile Industry","volume":"12","author":"Zille","year":"2015","journal-title":"Plasma Process. Polym."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hosne Asif, A.K.M.A., and Hasan, M.Z. (2018). Application of Nanotechnology in Modern Textiles: A Review. Int. J. Curr. Eng. Technol., 8.","DOI":"10.14741\/ijcet\/v.8.2.5"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-67139-7","article-title":"Multivalent and synergistic chitosan oligosaccharide-Ag nanocomposites for therapy of bacterial infection","volume":"10","author":"Mei","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3391","DOI":"10.1021\/j100214a025","article-title":"Adsorption and surface-enhanced Raman of dyes on silver and gold sols","volume":"86","author":"Lee","year":"1982","journal-title":"J. Phys. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"846","DOI":"10.4028\/www.scientific.net\/MSF.636-637.846","article-title":"Surface Modification on Polyamide 6.6 with Double Barrier Discharge (DBD) Plasma to Optimise Dyeing Process by Direct Dyes","volume":"636\u2013637","author":"Oliveira","year":"2010","journal-title":"Mater. Sci. Forum"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13731","DOI":"10.1021\/acsami.5b04340","article-title":"Size and Aging Effects on Antimicrobial Efficiency of Silver Nanoparticles Coated on Polyamide Fabrics Activated by Atmospheric DBD Plasma","volume":"7","author":"Zille","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.apsusc.2013.12.126","article-title":"Dyeing mechanism and optimization of polyamide 6,6 functionalized with double barrier discharge (DBD) plasma in air","volume":"293","author":"Oliveira","year":"2014","journal-title":"Appl. Surf. Sci."},{"key":"ref_25","first-page":"2685","article-title":"Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles","volume":"10","author":"Wang","year":"2015","journal-title":"Int. J. Nanomed."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"136765","DOI":"10.1155\/2015\/136765","article-title":"Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines","volume":"2015","author":"Sambale","year":"2015","journal-title":"J. Nanomater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"015101","DOI":"10.1088\/0957-4484\/24\/1\/015101","article-title":"Preparation and characterization of chitosan-silver nanocomposite films and their antibacterial activity against Staphylococcus aureus","volume":"24","author":"Regiel","year":"2013","journal-title":"Nanotechnology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1166\/jnn.2019.15863","article-title":"Silver\/Chitosan Antimicrobial Nanocomposites Coating for Medical Devices: Comparison of Nanofiller Effect Prepared via Chemical Reduction and Biosynthesis","volume":"19","author":"Pakseresht","year":"2019","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ned\u011bla, O., Slepi\u010dka, P., and \u0160vor\u010d\u00edk, V. (2017). Surface Modification of Polymer Substrates for Biomedical Applications. Materials, 10.","DOI":"10.3390\/ma10101115"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1016\/j.arabjc.2014.12.014","article-title":"Synthesis of silver nanoparticles with different shapes","volume":"12","author":"Khodashenas","year":"2019","journal-title":"Arab. J. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9849","DOI":"10.1166\/jnn.2015.10334","article-title":"Green Synthesis of Silver Nanoparticles: Effect of Dextran Molecular Weight Used as Stabilizing-Reducing Agent","volume":"15","year":"2015","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.carbpol.2012.03.089","article-title":"Synthesis, characterization and antimicrobial activity of dextran stabilized silver nanoparticles in aqueous medium","volume":"89","author":"Bankura","year":"2012","journal-title":"Carbohydr. Polym."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1016\/j.apsusc.2019.07.227","article-title":"Fabrication of Ag based ternary nanocomposite system for visible-light photocatalytic hydrogen evolution reaction","volume":"494","author":"Do","year":"2019","journal-title":"Appl. Surf. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4041","DOI":"10.1039\/C9NA00347A","article-title":"Silver nanoparticles by atomic vapour deposition on an alcohol micro-jet","volume":"1","author":"McNally","year":"2019","journal-title":"Nanoscale Adv."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jcis.2018.07.045","article-title":"Cyclodextrin-functionalized Ag\/AgCl foam with enhanced photocatalytic performance for water purification","volume":"531","author":"Zhu","year":"2018","journal-title":"J. Colloid Interface Sci."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Algarra, M., Campos, B.B., Radoti\u0107, K., Mutavd\u017ei\u0107, D., Bandosz, T., Jim\u00e9nez-Jim\u00e9nez, J., Rodriguez-Castell\u00f3n, E., and Esteves da Silva, J.C.G. (2014). Luminescent carbon nanoparticles: Effects of chemical functionalization, and evaluation of Ag+ sensing properties. J. Mater. Chem. A, 2.","DOI":"10.1039\/c4ta00264d"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"S552","DOI":"10.1093\/clinids\/7.Supplement_4.S552","article-title":"Nosocomial infections due to Gram-negative bacilli in compromised hosts: Considerations for prevention and therapy","volume":"7","author":"Klastersky","year":"1985","journal-title":"Rev. Infect. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"157","DOI":"10.2165\/11539770-000000000-00000","article-title":"Pseudomonas skin infection: Clinical features, epidemiology, and management","volume":"12","author":"Wu","year":"2011","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1080\/14760584.2016.1179583","article-title":"Staphylococcus aureus: The current state of disease, pathophysiology and strategies for prevention","volume":"15","author":"Dayan","year":"2016","journal-title":"Expert Rev. Vaccines"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1093\/femsre\/fux020","article-title":"Structure, function and regulation of Pseudomonas aeruginosa porins","volume":"41","author":"Chevalier","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.2147\/IJN.S191340","article-title":"Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa","volume":"14","author":"Liao","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Abdal Dayem, A., Hossain, M.K., Lee, S.B., Kim, K., Saha, S.K., Yang, G.-M., Choi, H.Y., and Cho, S.-G. (2017). The Role of Reactive Oxygen Species (ROS) in the Biological Activities of Metallic Nanoparticles. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18010120"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1038\/s41598-018-20728-z","article-title":"Silver nanoparticles have lethal and sublethal adverse effects on development and longevity by inducing ROS-mediated stress responses","volume":"8","author":"Mao","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Cameron, S.J., Hosseinian, F., and Willmore, W.G. (2018). A Current Overview of the Biological and Cellular Effects of Nanosilver. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19072030"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Sobhanifar, S., Worrall, L.J., King, D.T., Wasney, G.A., Baumann, L., Gale, R.T., Nosella, M., Brown, E.D., Withers, S.G., and Strynadka, N.C.J. (2016). Structure and Mechanism of Staphylococcus aureus TarS, the Wall Teichoic Acid \u03b2-glycosyltransferase Involved in Methicillin Resistance. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1006067"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"E576","DOI":"10.1073\/pnas.1418084112","article-title":"Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid \u03b1-glycosyltransferase","volume":"112","author":"Sobhanifar","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Sim, W., Barnard, R.T., Blaskovich, M.A.T., and Ziora, Z.M. (2018). Antimicrobial Silver in Medicinal and Consumer Applications: A Patent Review of the Past Decade (2007\u20132017). Antibiotics, 7.","DOI":"10.3390\/antibiotics7040093"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"46s","DOI":"10.1046\/j.1365-2672.92.5s1.7.x","article-title":"Cellular impermeability and uptake of biocides and antibiotics in Gram-positive bacteria and mycobacteria","volume":"92","author":"Lambert","year":"2002","journal-title":"J. Appl. Microbiol."}],"container-title":["Fibers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6439\/9\/1\/3\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:07:41Z","timestamp":1760159261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6439\/9\/1\/3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,6]]},"references-count":48,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["fib9010003"],"URL":"https:\/\/doi.org\/10.3390\/fib9010003","relation":{},"ISSN":["2079-6439"],"issn-type":[{"value":"2079-6439","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,6]]}}}