{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T20:25:40Z","timestamp":1774470340130,"version":"3.50.1"},"reference-count":72,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T00:00:00Z","timestamp":1577404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/Multi\/04326\/2019"],"award-info":[{"award-number":["UID\/Multi\/04326\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fishes"],"abstract":"<jats:p>Invasive species are recognized as a major cause of biodiversity decline. Legal regulations relating to the prevention, control, or eradication of invasive species should always be up-to-date, as the failure to recognize the problem, lack of adequate scientific information, or long legal intervals required to prepare the legislation may result in irreversible, possibly catastrophic, outcomes. This implies constant monitoring of the species distribution and levels of establishment, as well as detailed knowledge about its biology to predict dissemination and viability under changing environmental conditions. Pre-screening kits for potential invasive species are valuable tools for policy makers, as they provide information about if and how management measures should be taken. The Freshwater Fish Invasiveness Scoring Kit (FISK) and the Aquatic Species Invasiveness Screening Kit (AS-ISK) have been suggested as reliable tools to assess the potential risk of a species becoming invasive. The present study highlights the spread of the non-native chameleon cichlid Australoheros facetus in several streams of the major river drainages in southern Portugal and compares the fish assemblages and ecological indices in two selected sites in the Vasc\u00e3o and Odelouca rivers. We reviewed the current knowledge on the distribution, physiology, and behavior of A. facetus, and applied the toolkits FISK v2 and AS-ISK to this species to evaluate whether the species should be classified as invasive in Portugal. Field data show high abundance of the species in most streams and dominance in specific hotspots. The scores reached by the kits (FISK v2: 23; AS-ISK: 37) places A. facetus as a species with high potential of invasiveness and support the recent inclusion of this species in the invasive species list in Portugal (Decree-Law 92\/2019), but, most of all, highlights the importance of frequent updates in both the field monitoring and the legal regulation and watch lists of invasive organisms.<\/jats:p>","DOI":"10.3390\/fishes5010003","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T11:42:47Z","timestamp":1577446967000},"page":"3","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Distribution and Risk Assessment of Potential Invasiveness of Australoheros facetus (Jenyns, 1842) in Portugal"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4179-8111","authenticated-orcid":false,"given":"Flavia","family":"Baduy","sequence":"first","affiliation":[{"name":"CCMAR-Centro de Ci\u00eancias do Mar, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8891-8881","authenticated-orcid":false,"given":"Jo\u00e3o L.","family":"Saraiva","sequence":"additional","affiliation":[{"name":"CCMAR-Centro de Ci\u00eancias do Mar, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"given":"Filipe","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"MARE-Centro de Ci\u00eancias do Mar e do Ambiente, Universidade de Lisboa, 1600-548 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6244-6468","authenticated-orcid":false,"given":"Adelino V. M.","family":"Canario","sequence":"additional","affiliation":[{"name":"CCMAR-Centro de Ci\u00eancias do Mar, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5371-7919","authenticated-orcid":false,"given":"Pedro M.","family":"Guerreiro","sequence":"additional","affiliation":[{"name":"CCMAR-Centro de Ci\u00eancias do Mar, Universidade do Algarve, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10530-005-0231-3","article-title":"Global documentation of fish introductions: The growing crisis and recommendations for action","volume":"8","author":"Casal","year":"2006","journal-title":"Biol. Invasions"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.tree.2005.01.003","article-title":"Invasive species are a leading cause of animal extinctions","volume":"20","author":"Clavero","year":"2005","journal-title":"Trends Ecol. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/gcb.13004","article-title":"Global ecological impacts of invasive species in aquatic ecosystems","volume":"22","author":"Gallardo","year":"2016","journal-title":"Glob. Chang. Biol."},{"key":"ref_4","unstructured":"International Union for Conservation of Nature (2000). Guidelines for the Prevention of Biodiversity Loss Due to Biological Invasion, IUCN."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1890\/1051-0761(2000)010[0689:BICEGC]2.0.CO;2","article-title":"Biotic invasions: Causes, epidemiology, global consequences, and control","volume":"10","author":"Mack","year":"2002","journal-title":"Ecol. Appl."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Epanchin-Niell, R.S. (2017). Economics of invasive species policy and management. Biol. Invasions.","DOI":"10.1007\/s10530-017-1406-4"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1111\/j.1461-0248.2010.01440.x","article-title":"Controlling established invaders: Integrating economics and spread dynamics to determine optimal management","volume":"13","author":"Hastings","year":"2010","journal-title":"Ecol. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1080\/14634988.2015.1046357","article-title":"Risk assessment: Cornerstone of an aquatic invasive species program","volume":"18","author":"Mandrak","year":"2015","journal-title":"Aquat. Ecosyst. Health Manag."},{"key":"ref_9","unstructured":"Drake, J.A. (1989). Biological Invasions: A Global Perspective, Scientific Committee on Problems of the Environment."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1111\/j.1365-2427.2011.02642.x","article-title":"Assessing the risk of freshwater fish introductions into the Iberian Peninsula","volume":"56","author":"Clavero","year":"2011","journal-title":"Freshw. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s10530-004-9640-y","article-title":"Life-history traits of invasive fish in small Mediterranean streams","volume":"7","author":"Alcaraz","year":"2005","journal-title":"Biol. Invasions"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s13280-012-0379-x","article-title":"Conceptual frameworks and methods for advancing invasion ecology","volume":"42","author":"Heger","year":"2013","journal-title":"Ambio"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1006\/jema.1999.0297","article-title":"A weed risk assessment model for use as a biosecurity tool evaluating plant introductions","volume":"57","author":"Pheloung","year":"1999","journal-title":"J. Environ. Manag."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1111\/j.1439-0426.2005.00692.x","article-title":"Risk identification and assessment of non-native freshwater fishes: A summary of concepts and perspectives on protocols for the UK","volume":"21","author":"Copp","year":"2005","journal-title":"J. Appl. Ichthyol."},{"key":"ref_15","unstructured":"Copp, G.H., Britton, J.R., Jeney, G., Joly, J.-P., Gherardi, F., Gollasch, S., Gozlan, R.E., Jones, G., MacLeod, A., and Midtlyng, P.J. (2008). Risk Assessment Protocols and Decision Making Tools for Use of Alien Species in Aquaculture and Stock Enhancement, IMPASSE\u2014Environmental Impacts of Alien Species in Aquaculture for Coordination Action Priority FP6 2005-SSP-5A, Sustainable Management of Europe\u2019s Natural Resources, Project No.: 044142."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1111\/risa.12095","article-title":"The Fish Invasiveness Screening Kit (FISK) for non-native freshwater fishes\u2014A summary of current applications","volume":"33","author":"Copp","year":"2013","journal-title":"Risk Anal."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1111\/j.1539-6924.2012.01896.x","article-title":"Revisions of the Fish Invasiveness Screening Kit (FISK) for its application in warmer climatic zones, with particular reference to peninsular Florida","volume":"33","author":"Lawson","year":"2013","journal-title":"Risk Anal."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1111\/risa.12050","article-title":"Effectiveness of FISK, an invasiveness screening tool for non-native freshwater fishes, to perform risk identification assessments in the Iberian Peninsula","volume":"33","author":"Almeida","year":"2013","journal-title":"Risk Anal."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"343","DOI":"10.3391\/mbi.2016.7.4.04","article-title":"Development of a generic decision-support tool for identifying potentially invasive aquatic taxa: AS-ISK","volume":"7","author":"Copp","year":"2016","journal-title":"Manag. Biol. Invasions"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Glamuzina, B., Tutman, P., Nikoli\u0107, V., Vidovi\u0107, Z., Pavli\u010devi\u0107, J., Vilizzi, L., Copp, G.H., and Simonovi\u0107, P. (2017). Comparison of taxon-specific and taxon-generic risk screening tools to identify potentially invasive non-native fishes in the river Neretva catchment (Bosnia and Herzegovina and Croatia). River Res. Appl.","DOI":"10.1002\/rra.3124"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1080\/09397140.2017.1269398","article-title":"Risk screening of non-native and translocated freshwater fish species in a Mediterranean-type shallow lake: Lake Marmara (West Anatolia)","volume":"63","author":"Tarkan","year":"2016","journal-title":"Zool. Middle East"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Tarkan, A.S., Vilizzi, L., Top, N., Ekmek\u00e7i, F.G., Stebbing, P.D., and Copp, G.H. (2017). Identification of potentially invasive freshwater fishes, including translocated species, in Turkey using the Aquatic Species Invasiveness Screening Kit (AS-ISK). Int. Rev. Hydrobiol.","DOI":"10.1002\/iroh.201601877"},{"key":"ref_23","unstructured":"(2019, December 19). FishBase. Available online: www.fishbase.org."},{"key":"ref_24","unstructured":"(2019, December 22). Available online: https:\/\/data.dre.pt\/eli\/dec-lei\/565\/1999\/12\/21\/p\/dre\/pt\/html."},{"key":"ref_25","unstructured":"(2019, December 22). Available online: https:\/\/data.dre.pt\/eli\/dec-lei\/92\/2019\/07\/10\/p\/dre."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1439-0469.2005.00347.x","article-title":"Character- and tree-based delimitation of species in the \u2018Cichlasoma\u2019 facetum group (Teleostei, Cichlidae) with the description of a new genus","volume":"44","author":"Kullander","year":"2006","journal-title":"J. Zool. Syst. Evol. Res."},{"key":"ref_27","first-page":"12","article-title":"Taxonomic re-evaluation of the non-native cichlid in Portuguese drainages","volume":"1","author":"Carecho","year":"2018","journal-title":"Fishes Mediterr. Environ."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Baduy, F., Guerreiro, P.M., Can\u00e1rio, A.V., and Saraiva, J.L. (2017). Social organization and endocrine profiles of Australoheros facetus, an exotic freshwater fish in southern Portugal. Acta Ethologica.","DOI":"10.1007\/s10211-017-0271-6"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1111\/j.1600-0633.2007.00252.x","article-title":"Variability in feeding ecology of a South American cichlid: A reason for successful invasion in mediterranean-type rivers?","volume":"16","author":"Ribeiro","year":"2008","journal-title":"Ecol. Freshw. Fish"},{"key":"ref_30","first-page":"193","article-title":"Aspectos biologicos del pez exotico Cichlasoma facetum (Jenyns, 1842) (Pisces, Cichlidae) en aguas dulces de Concepcion","volume":"63","author":"Ruiz","year":"1992","journal-title":"Bol. Soc. Biol. Concepc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3391\/ai.2010.5.3.03","article-title":"Impacts of non-native fishes on Iberian freshwater ichthyofauna: Current knowledge and gaps","volume":"5","author":"Leunda","year":"2010","journal-title":"Aquat. Invasions"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"S\u00e1ez-G\u00f3mez, P., and Prenda, J. (2019). Updating the distribution data of recently introduced freshwater fish in the Guadalquivir River Basin (Spain). Bioinvasions Rec., 8.","DOI":"10.3391\/bir.2019.8.4.21"},{"key":"ref_33","unstructured":"(2019, October 28). BioDiversit4All. Available online: www.biodiversity4all.org."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1146\/annurev.ecolsys.39.110707.173451","article-title":"Plague Minnow or Mosquito Fish? A Review of the Biology and Impacts of Introduced Gambusia Species","volume":"39","author":"Pyke","year":"2008","journal-title":"Annu. Rev. Ecol. Evol. Syst."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1071\/WR12172","article-title":"Removal control of the highly invasive fish Gambusia holbrooki and effects on its population biology: Learning by doing","volume":"40","author":"Torralva","year":"2013","journal-title":"Wildl. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.scitotenv.2019.02.460","article-title":"Managing small, highly prolific invasive aquatic species: Exploring anecosystem approach for the eastern mosquitofish (Gambusia holbrooki)","volume":"673","author":"Coll","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1890\/09-2011.1","article-title":"Invasive species and habitat degradation in Iberian streams: An analysis of their role in freshwater fish diversity loss","volume":"21","author":"Hermoso","year":"2011","journal-title":"Ecol. Appl."},{"key":"ref_38","first-page":"283","article-title":"First record of Ameiurus melas (Siluriformes, Ictaluridae) in the Alqueva reservoir, Guadiana basin (Portugal)","volume":"30","author":"Ribeiro","year":"2006","journal-title":"Cybium"},{"key":"ref_39","first-page":"3","article-title":"Univited dinner guests: The effect of invasive fish and temperature on the foraging efficiency of Southern Iberian Chub","volume":"11","author":"Kodde","year":"2016","journal-title":"Fishes Mediterr. Environ."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/j.1365-2400.2009.00659.x","article-title":"Non-native fish in the fresh waters of Portugal, Azores and Madeira Islands: A growing threat to aquatic biodiversity","volume":"16","author":"Ribeiro","year":"2009","journal-title":"Fish. Manag. Ecol."},{"key":"ref_41","first-page":"357","article-title":"Fish assemblage structure across the Arade basin (southern Portugal)","volume":"28","author":"Pires","year":"2004","journal-title":"Cybium"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1111\/j.1600-0633.2009.00391.x","article-title":"Variation in fish assemblages across dry-season pools in a Mediterranean stream: Effects of pool morphology, physicochemical factors and spatial context","volume":"19","author":"Pires","year":"2010","journal-title":"Ecol. Freshw. Fish"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s10641-012-0030-5","article-title":"Spatial and temporal variation in assemblage structure of fish larvae in mediterranean-type streams: Contrasts between native and non-native species","volume":"96","author":"Ribeiro","year":"2013","journal-title":"Environ. Biol. Fishes"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.1095-8649.2007.01668.x","article-title":"The characteristics of invasive fishes: What has been learned so far?","volume":"71","year":"2007","journal-title":"J. Fish Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1016\/j.scitotenv.2018.09.251","article-title":"Non-native freshwater fauna in Portugal: A review","volume":"650","author":"Ribeiro","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1577\/T05-025.1","article-title":"Comparison of upper thermal tolerances of native and non-native fish species in Arizona","volume":"135","author":"Carveth","year":"2001","journal-title":"Trans. Am. Fish. Soc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s10750-016-2740-8","article-title":"Salinity and temperature tolerance of an emergent alien species, the Amazon fish Astronotus ocellatus","volume":"777","author":"Gutierre","year":"2016","journal-title":"Hydrobiologia"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1095-8649.1999.tb00827.x","article-title":"Seasonal changes in fish community structure of intermittent streams in the middle reaches of the Guadiana basin, Portugal","volume":"54","author":"Pires","year":"1999","journal-title":"J. Fish Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1046\/j.1365-2427.2002.00941.x","article-title":"Functional heterogeneity of dry-season fish refugia across a Mediterranean catchment: The role of habitat and predation","volume":"47","author":"Magalhaes","year":"2002","journal-title":"Freshw. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Baduy, F., Soares, J., Silva, M., Canario, A.V.M., Saraiva, J.L., and Guerreiro, P.M. (2016). Critical thermal maximum and minimum in Australoheros facetus, a neotropical invader in the Iberian Peninsula. Fishes Mediterr. Environ., 12.","DOI":"10.29094\/FiSHMED.2016.012"},{"key":"ref_51","unstructured":"Baduy, F., Saraiva, J.L., Vargas, M., Silva, M., Soares, J., Can\u00e1rio, A.V.M., and Guerreiro, P.M. (2016). Plastic physiology, social behaviour and endocrine profiles of an invasive cichlid in Southern Portugal. Adv. Comp. Endocrinol."},{"key":"ref_52","unstructured":"Baduy, F., Teixeira, D., Soares, D., Saraiva, J.L., Can\u00e1rio, A.V.M., and Guerreiro, P.M. (2016, January 21). Dispersion of the invasive Australoheros facetus into upper estuaries may be deterred by salinity-related effects on both physiology and behaviour. Proceedings of the VI Iberian Congress of Ichthyology, Murcia, Spain."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.ecolind.2012.04.024","article-title":"Effects of natural hydrological variability on fish assemblages in small Mediterranean streams: Implications for ecological assessment","volume":"23","author":"Matono","year":"2012","journal-title":"Ecol. Indic."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Alexandre, C.M., Ferreira, T.F., and Almeida, P.R. (2012). Fish assemblages in non-regulated and regulated rivers from permanent and temporary Iberian systems. River Res. Appl.","DOI":"10.1002\/rra.2591"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1002\/rra.726","article-title":"Interannual variation of fish assemblage structure in a Mediterranean River: Implications of streamflow on the dominance of native or exotic species","volume":"19","author":"Bernardo","year":"2003","journal-title":"River Res. Appl."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1600-0633.1997.tb00155.x","article-title":"Composition and spatial organization of fish assemblages in the lower Guadiana basin, southern Iberia","volume":"6","author":"Godinho","year":"1997","journal-title":"Ecol. Freshw. Fish"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1111\/j.1095-8649.2009.02506.x","article-title":"Life-history variability of non-native centrarchids in regulated river systems of the lower River Guadiana drainage (south-west Iberian Peninsula)","volume":"76","author":"Ribeiro","year":"2010","journal-title":"J. Fish Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s10530-007-9112-2","article-title":"Life-history traits of non-native fishes in Iberian watersheds across several invasion stages: A first approach","volume":"10","author":"Ribeiro","year":"2008","journal-title":"Biol. Invasions"},{"key":"ref_59","unstructured":"Range, I.L. (2013). Applicability of Fish Risk Assessment (FISK) to Ornamental Species. [Master\u2019s Thesis, University of Lisbon]."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Oliveira, J.M., Segurado, P., Santos, J.M., Teixeira, A., Ferreira, M.T., and Cortes, R.V. (2012). Modelling stream-fish functional traits in reference conditions: Regional and local environmental correlates. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0045787"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1146\/annurev.ecolsys.30.1.51","article-title":"Streams in Mediterranean climate regions: Abiotic influences and biotic responses to predictable seasonal events","volume":"30","author":"Gasith","year":"1999","journal-title":"Annu. Rev. Ecol. Evol. Syst."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/czoolo\/60.3.417","article-title":"Animal personality and the ecological impacts of freshwater non-native species","volume":"60","author":"Juette","year":"2014","journal-title":"Curr. Zool."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1890\/02-5301","article-title":"Alien fishes in California watersheds: Characteristics of successful and failed invaders","volume":"14","author":"Marchetti","year":"2004","journal-title":"Ecol. Appl."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s11160-014-9375-5","article-title":"Life-history traits of non-native freshwater fish invaders differentiate them from natives in the Central European bioregion","volume":"25","author":"Grabowska","year":"2014","journal-title":"Rev. Fish Biol. Fish."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0376-6357(88)90047-2","article-title":"Dynamics of nest defense by male centrarchid fish","volume":"17","author":"Colgan","year":"1998","journal-title":"Behav. Process."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/s11160-019-09562-2","article-title":"A global review and meta-analysis of applications of the freshwater Fish Invasiveness Screening Kit","volume":"29","author":"Vilizzi","year":"2019","journal-title":"Rev. Fish Biol. Fish."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1111\/j.1365-2427.2010.02396.x","article-title":"Non-nativefishes and climate change: Predicting species responses towarming temperatures in a temperate region","volume":"55","author":"Britton","year":"2010","journal-title":"Freshw. Biol."},{"key":"ref_68","unstructured":"(2019, November 01). Real Decreto 630\/2013 del Reyno de Espa\u00f1a. Available online: https:\/\/www.boe.es\/eli\/es\/rd\/2013\/08\/02\/630."},{"key":"ref_69","unstructured":"(2019, December 22). Define as Bases da Pol\u00edtica de Ambiente; Di\u00e1rio da Rep\u00fablica Lisbon, Portuguesa; Lei (n\u00ba 19\/2014), I S\u00e9rie, n\u00ba 73. Available online: https:\/\/data.dre.pt\/eli\/lei\/19\/2014\/04\/14\/p\/dre\/pt\/html."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s13280-015-0718-9","article-title":"What do we really know about the impacts of one of the 100 worst invaders in Europe? A reality check","volume":"45","author":"Hirsch","year":"2016","journal-title":"Ambio"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.1365-2400.2011.00842.x","article-title":"Non-native fish impacts on Mediterranean freshwater ecosystems: Current knowledge and research needs","volume":"19","author":"Ribeiro","year":"2012","journal-title":"Fish. Manag. Ecol."},{"key":"ref_72","unstructured":"(2018, February 05). Cefas\u2014Centre for Environment, Fisheries and Aquaculture Science. Available online: www.cefas.co.uk."}],"container-title":["Fishes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2410-3888\/5\/1\/3\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:46:07Z","timestamp":1760190367000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2410-3888\/5\/1\/3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,27]]},"references-count":72,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["fishes5010003"],"URL":"https:\/\/doi.org\/10.3390\/fishes5010003","relation":{},"ISSN":["2410-3888"],"issn-type":[{"value":"2410-3888","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,27]]}}}