{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T17:00:20Z","timestamp":1775494820564,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T00:00:00Z","timestamp":1621555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PTDC\/CVT-NUT\/5931\/2014"],"award-info":[{"award-number":["PTDC\/CVT-NUT\/5931\/2014"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UIDB\/00276\/2020"],"award-info":[{"award-number":["UIDB\/00276\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BD\/126198\/2016"],"award-info":[{"award-number":["SFRH\/BD\/126198\/2016"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BPD\/116816\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/116816\/2016"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BD\/143992\/2019"],"award-info":[{"award-number":["SFRH\/BD\/143992\/2019"]}]},{"name":"Portugal2020","award":["08\/SI\/3399\/2015"],"award-info":[{"award-number":["08\/SI\/3399\/2015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Chlorella vulgaris (CH) is usually considered a feed supplement in pig nutrition, and its use as an ingredient is poorly studied. Among many interesting characteristics, this microalga has high protein levels and can be a putative alternative for soybean meal. Our aim was to study the effect of a 5% CH incorporation in the diet, individually or combined with two carbohydrases, on meat quality traits and nutritional value. Forty-four post-weaned male piglets individually housed, with an initial live weight of 11.2 \u00b1 0.46 kg, were randomly distributed into four experimental groups: control (n = 11, without CH) and three groups fed with 5% CH incorporation, plain (n = 10), with 0.005% Rovabio\u00ae Excel AP (n = 10), and with 0.01% of a pre-selected four-CAZyme mixture (n = 11). After two weeks of trial, piglets were slaughtered and longissimus lumborum collected. CH had no effect on piglets\u2019 growth performance. In turn, incorporation of CH improved the nutritional value of meat by increasing total carotenoids and n-3 PUFA content, thus contributing to a more positive n-6\/n-3 fatty acid ratio. The supplementation with Rovabio\u00ae benefited tenderness and increased overall acceptability of pork. Our results show beyond doubt the viability of the utilization of this microalga as a feed ingredient for swine production.<\/jats:p>","DOI":"10.3390\/foods10061155","type":"journal-article","created":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T13:15:15Z","timestamp":1621602915000},"page":"1155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Effects of Chlorella vulgaris as a Feed Ingredient on the Quality and Nutritional Value of Weaned Piglets\u2019 Meat"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1017-3269","authenticated-orcid":false,"given":"C\u00e1tia F.","family":"Martins","sequence":"first","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisbon, Portugal"},{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"given":"Jos\u00e9 M.","family":"Pestana","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8648-2204","authenticated-orcid":false,"given":"Cristina M.","family":"Alfaia","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3030-7789","authenticated-orcid":false,"given":"M\u00f3nica","family":"Costa","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0990-7739","authenticated-orcid":false,"given":"David M.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6151-8186","authenticated-orcid":false,"given":"Diogo","family":"Coelho","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6755-4572","authenticated-orcid":false,"given":"Paula A.","family":"Lopes","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisbon, Portugal"}]},{"given":"Andr\u00e9 M.","family":"Almeida","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"given":"Jo\u00e3o P. B.","family":"Freire","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1032-5987","authenticated-orcid":false,"given":"Jos\u00e9 A. M.","family":"Prates","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, 1300-477 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,21]]},"reference":[{"key":"ref_1","unstructured":"Food and Agriculture Organization of the United Nations (FAO) (2017). The Future of Food and Agriculture: Trends and Challenges, FAO."},{"key":"ref_2","unstructured":"Food and Agriculture Organization of the United Nations (FAO) (2007). The State of Food and Agriculture, FAO."},{"key":"ref_3","unstructured":"Food and Agriculture Organization of the United Nations (FAO) (2011). Food Insecurity in the World, FAO."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.livsci.2017.09.020","article-title":"Microalgae as feed ingredients for livestock production and meat quality: A review","volume":"205","author":"Madeira","year":"2017","journal-title":"Livest. Sci."},{"key":"ref_5","unstructured":"Galanakis, C.M. (2020). Food applications. Microalgae: Cultivation, Recovery of Compounds and Applications Edited, Elsevier Science. [1st ed.]."},{"key":"ref_6","unstructured":"Galanakis, C.M. (2020). Cultivation techniques. Microalgae: Cultivation, Recovery of Compounds and Applications Edited, Elsevier Science. [1st ed.]."},{"key":"ref_7","unstructured":"Sander, K., and Murthy, G.S. (2009, January 21\u201324). Enzymatic degradation of microalgal cell walls. Proceedings of the American Society of Agricultural and Biological Engineers (ASEBE), Annual International Meeting, St. Joseph, NV, USA."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/jpn.13470","article-title":"Effect of dietary inclusion of Spirulina on production performance, nutrient digestibility and meat quality traits in post-weaning piglets","volume":"105","author":"Martins","year":"2021","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Coelho, D., Pestana, J., Almeida, J.M., Alfaia, C.M., Fontes, C.M.G.A., Moreira, O., and Prates, J.A.M. (2020). A High Dietary incorporation level of Chlorella vulgaris improves the nutritional value of pork fat without impairing the performance of finishing pigs. Animals, 10.","DOI":"10.20944\/preprints202011.0253.v1"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5382","DOI":"10.1038\/s41598-019-41775-0","article-title":"Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall","volume":"9","author":"Coelho","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1017\/S1751731116001543","article-title":"Effects of dietary supplementation with freshwater microalgae on growth performance, nutrient digestibility and gut health in weaned piglets","volume":"11","author":"Furbeyre","year":"2017","journal-title":"Animal"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2264","DOI":"10.1017\/S1751731118000125","article-title":"Effects of oral supplementation with Spirulina and Chlorella on growth and digestive health in piglets around weaning","volume":"12","author":"Furbeyre","year":"2018","journal-title":"Animal"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0377-8401(99)00130-3","article-title":"Effects of Spirulina platensis on growth performance of weanling pigs","volume":"83","author":"Grinstead","year":"2000","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.livsci.2010.05.007","article-title":"Effect of piglet birth weight on survival and quality of commercial market swine","volume":"132","author":"Fix","year":"2010","journal-title":"Livest. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.aninu.2017.01.001","article-title":"Post-weaning and whole-of-life performance of pigs is determined by live weight at weaning and the complexity of the diet fed after weaning","volume":"3","author":"Collins","year":"2017","journal-title":"Anim. Nutr."},{"key":"ref_16","unstructured":"Association of Official Analytical Chemists International (2000). Official Methods of Analysis, AOAC International. [17th ed.]."},{"key":"ref_17","unstructured":"Noblet, J., Fortune, H., Dubois, S., and Henry, Y. (1989). Nouvelles Bases D\u2019estimation des Teneurs en \u00e9nergie Digestible M\u00e9tabolisable et Nette des Aliments Pour le Porc, INRA."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1021\/jf00084a019","article-title":"Rapid method for determination of total fatty acid content and composition of feedstufs and feces","volume":"36","author":"Sukhija","year":"1988","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.foodchem.2005.01.021","article-title":"Simultaneous HPLC quantification of total cholesterol, tocopherols and carotene in Barros\u00e3-PDO veal","volume":"94","author":"Prates","year":"2006","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.aquaculture.2013.02.009","article-title":"The effects of Spirulina platensis meal as a feed supplement on growth performance and pigmentation of rainbow trout (Oncorhynchus mykiss)","volume":"396","author":"Teimouri","year":"2013","journal-title":"Aquaculture"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jpba.2017.09.018","article-title":"Separation, identification and quantification of carotenoids and chlorophylls in dietary supplements containing Chlorella vulgaris and Spirulina platensis using High Performance Thin Layer Chromatography","volume":"148","author":"Hynstova","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1021\/jf990518q","article-title":"Measurement of 2-thiobarbituric acid values in dark chicken meat through derivative spectrophotometry: Influence of various parameters","volume":"48","author":"Grau","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","first-page":"48","article-title":"Training and testing of judges for sensory analysis of meat quality","volume":"32","author":"Cross","year":"1979","journal-title":"Food Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0009-8981(85)90277-3","article-title":"Extraction of lipids from human whole serum and lipoproteins and from rat liver tissue with methylene chloride-methanol: A comparison with extraction with chloroform-methanol","volume":"149","author":"Carlson","year":"1985","journal-title":"Clin. Chim. Acta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1017\/S1357729800058227","article-title":"Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids","volume":"73","author":"Raes","year":"2001","journal-title":"Anim. Sci."},{"key":"ref_27","first-page":"1742","article-title":"Effect of fermented Chlorella supplementation on growth performance, nutrient digestibility, blood characteristics, fecal Microbial and fecal noxious gas content in growing pigs. Asian Australas","volume":"25","author":"Yan","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.meatsci.2007.07.019","article-title":"Fat deposition, fatty acid composition and meat quality: A review","volume":"78","author":"Wood","year":"2008","journal-title":"Meat Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0309-1740(03)00018-4","article-title":"Comparison of antioxidant effects of milk mineral, butylated hydroxytoluene and sodium tripolyphosphate in raw and cooked ground pork","volume":"66","author":"Jayasingh","year":"2003","journal-title":"Meat Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0950-3293(02)00086-1","article-title":"Cooking loss and juiciness of pork in relation to raw meat quality and cooking procedure","volume":"14","author":"Aaslyng","year":"2003","journal-title":"Food Qual. Prefer."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s11883-019-0762-1","article-title":"New insights into mechanisms of action for omega-3 fatty acids in atherothrombotic cardiovascular disease","volume":"21","author":"Mason","year":"2019","journal-title":"Curr. Atheroscler. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jjcc.2015.08.002","article-title":"Cardioprotective mechanism of omega-3 polyunsaturated fatty acids. Review","volume":"67","author":"Endo","year":"2016","journal-title":"J. Cardiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"171","DOI":"10.14797\/mdcj-15-3-171","article-title":"Recent clinical trials shed new light on the cardiovascular benefits of omega-3 fatty acids","volume":"15","author":"Richter","year":"2019","journal-title":"Methodist Debakey Cardiovasc. J."},{"key":"ref_34","first-page":"364040","article-title":"Why and how meet n-3 PUFA dietary recommendations?","volume":"2011","author":"Legry","year":"2011","journal-title":"Gastroenterol. Res. Pract."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"S8","DOI":"10.1017\/S0007114512001444","article-title":"Recommended dietary reference intakes, nutritional goals and dietary guidelines for fat andatty acids: A systematic review","volume":"107","author":"Aranceta","year":"2012","journal-title":"Br. J. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.3390\/md11072259","article-title":"Alternative sources of n-3 long-chain polyunsaturated fatty acids in marine microalgae","volume":"11","author":"Martins","year":"2013","journal-title":"Rev. Mar. Drugs"},{"key":"ref_37","unstructured":"HMSO (1994). Nutritional Aspects of Cardiovascular Disease."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/6\/1155\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:05:21Z","timestamp":1760162721000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/6\/1155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,21]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["foods10061155"],"URL":"https:\/\/doi.org\/10.3390\/foods10061155","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,21]]}}}