{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:31:40Z","timestamp":1777426300771,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Prunus avium L. by-products, such as stems, leaves, and flowers, are used in folk medicine to prevent and treat some diseases. However, their phenolic composition and in vitro bioactivities against tumor cells are poorly known. In this work, we compared the phenolic profile and the biological potential of aqueous infusions and hydroethanolic extracts of P. avium leaves, stems, and flowers from Saco cultivar, collected from the Fund\u00e3o region (Portugal). Among the fifty-two phenolic compounds tentatively identified by HPLC-DAD-ESI\/MSn, the hydroxycinnamic acids were the most abundant. Both extracts of stems revealed a higher activity against DPPH\u2022. Meanwhile, hydroethanolic extracts from stems and flowers and aqueous infusions of flowers were the most effective in inhibiting the growth of the human epithelial colorectal adenocarcinoma (Caco-2) cells at concentrations above 200 \u03bcg\/mL. More detailed knowledge about the phenolic composition and health-promoting properties of Portuguese P. avium by-products allows for increasing the biological and commercial value of these bio-wastes, which may have a positive impact on food and pharmaceutical industries, as on the valorization of the local economy.<\/jats:p>","DOI":"10.3390\/foods10061185","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T02:53:36Z","timestamp":1621911216000},"page":"1185","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Valorisation of Prunus avium L. By-Products: Phenolic Composition and Effect on Caco-2 Cells Viability"],"prefix":"10.3390","volume":"10","author":[{"given":"Ana R.","family":"Nunes","sequence":"first","affiliation":[{"name":"CICS-UBI\u2014Health Sciences Research Center, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"},{"name":"CNC\u2014Centre for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7795-795X","authenticated-orcid":false,"given":"Ana C.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"CICS-UBI\u2014Health Sciences Research Center, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"},{"name":"Laboratory of Pharmacology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4213-0714","authenticated-orcid":false,"given":"Gilberto","family":"Alves","sequence":"additional","affiliation":[{"name":"CICS-UBI\u2014Health Sciences Research Center, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3854-6549","authenticated-orcid":false,"given":"Am\u00edlcar","family":"Falc\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"CIBIT\u2014Coimbra Institute for Biomedical Imaging and Translational Research, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-3917","authenticated-orcid":false,"given":"Cristina","family":"Garcia-Viguera","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Foods Laboratory, Food Science and Technology Department, CEBAS-CSIC, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6547-8764","authenticated-orcid":false,"given":"Diego","family":"A. Moreno","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Foods Laboratory, Food Science and Technology Department, CEBAS-CSIC, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5264-3516","authenticated-orcid":false,"given":"Lu\u00eds R.","family":"Silva","sequence":"additional","affiliation":[{"name":"CICS-UBI\u2014Health Sciences Research Center, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Nutrients, Bioactive Compounds and Bioactivity: The Health Benefits of Sweet Cherries (Prunus avium L.)","volume":"14","author":"Bento","year":"2018","journal-title":"Curr. Nutr. Food Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1016\/j.foodchem.2014.10.145","article-title":"Chemical characterisation and bioactive properties of Prunus avium L.: The widely studied fruits and the unexplored stems","volume":"173","author":"Bastos","year":"2015","journal-title":"Food. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.foodres.2017.02.023","article-title":"Sweet cherries from Fund\u00e3o possess antidiabetic potential and protect human erythrocytes against oxidative damage","volume":"95","author":"Bento","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, A., Rodrigues, M., Santos, A., Alves, G., and Silva, L.R. (2018). Antioxidant Status, Antidiabetic Properties and Effects on Caco-2 Cells of Colored and Non-Colored Enriched Extracts of Sweet Cherry Fruits. Nutrients, 10.","DOI":"10.20944\/preprints201809.0414.v1"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"127637","DOI":"10.1016\/j.foodchem.2020.127637","article-title":"Physical and phytochemical compositon of 23 Portuguese sweet cherries as conditioned by variety (or genotype)","volume":"335","author":"Campos","year":"2021","journal-title":"Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.foodres.2017.03.030","article-title":"Phenolic compounds profile and antioxidant properties of six sweet cherry (Prunus avium) cultivars","volume":"97","author":"Martini","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1080\/01635581.2019.1661502","article-title":"Sweet Cherry Extract Targets the Hallmarks of Cancer in Prostate Cells: Diminished Viability, Increased Apoptosis and Suppressed Glycolytic Metabolism","volume":"72","author":"Silva","year":"2020","journal-title":"Nutr. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/j.supflu.2010.06.006","article-title":"Processing cherries (Prunus avium) using supercritical fluid technology. Part 2. Evaluation of SCF extracts as promising natural chemotherapeutical agent","volume":"55","author":"Serra","year":"2011","journal-title":"J. Supercrit. Fluids"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.foodres.2018.08.079","article-title":"Exploring the phenolic profile, antioxidant, antidiabetic and anti-hemolytic potential of Prunus avium vegetal parts","volume":"116","author":"Jesus","year":"2019","journal-title":"Food. Res. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.jff.2018.02.018","article-title":"Assessing the phenolic profile, antioxidant, antidiabetic and protective effects against oxidative damage in human erythrocytes of peaches from Fund\u00e3o","volume":"43","author":"Bento","year":"2018","journal-title":"J. Funct. Foods"},{"key":"ref_11","first-page":"198","article-title":"Phenolic compounds in sweet cherry (Prunus avium L.) petioles and their antioxidant properties","volume":"43","author":"Prvulovic","year":"2011","journal-title":"Res. J. Agric. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1016\/j.fm.2010.12.008","article-title":"Exploitation of sweet cherry (Prunus avium L.) puree added of stem infusion through fermentation by selected autochthonous lactic acid bacteria","volume":"28","author":"Cagno","year":"2011","journal-title":"Food Microbiol."},{"key":"ref_13","first-page":"381","article-title":"Diuretic effect of powdered Cerasus avium (cherry) tails on healthy volunteers","volume":"22","author":"Hooman","year":"2009","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_14","first-page":"1","article-title":"Sour Cherry By-products: Compositions, Functional Properties and Recovery Potentials\u2014A Review","volume":"59","author":"Karaaslan","year":"2019","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1016\/j.foodchem.2009.01.056","article-title":"A new drink rich in healthy bioactives combining lemon and pomegranate juices","volume":"115","author":"Moreno","year":"2009","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"16428","DOI":"10.3390\/molecules191016428","article-title":"Stumps of Eucalyptus globulus as a source of antioxindar and antimicrobial polyphenols","volume":"19","author":"Neiva","year":"2014","journal-title":"Molecules"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Migues, I., Baenas, N., Giron\u00e9s-Vilaplana, A., Cesio, M.V., Heinzen, H., and Moreno, D.A. (2018). Phenolic Profiling and Antioxidant Capacity of Eugenia uniflora L. (Pitanga) Samples Collected in Different Uruguayan Locations. Foods, 7.","DOI":"10.3390\/foods7050067"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jpba.2013.08.040","article-title":"Assessing Jasminum grandiflorum L. authenticity by HPLC-DAD-ESI\/MSn and effects on physiological enzymes and oxidative species","volume":"88","author":"Ferreres","year":"2014","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/j.foodchem.2007.09.030","article-title":"Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit","volume":"107","author":"Barreira","year":"2008","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.foodchem.2014.01.125","article-title":"Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits","volume":"156","author":"Souza","year":"2014","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.foodchem.2010.07.088","article-title":"Identification of bioactive response in traditional cherries from Portugal","volume":"125","author":"Serra","year":"2011","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8813","DOI":"10.3390\/molecules15128813","article-title":"Phenolic-compound-extraction systems for fruit and vegetable samples","volume":"15","year":"2010","journal-title":"Molecules"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.tifs.2005.12.004","article-title":"Recent advances in extraction of nutraceuticals from plants","volume":"17","author":"Wang","year":"2006","journal-title":"Trends Food Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1007\/s11694-020-00698-5","article-title":"Valorization of fruit processing by-products: Free, esterifed, and insoluble bound phytochemical extraction from cherry (Prunus avium) tissues and their biological activities","volume":"15","author":"Pekmez","year":"2021","journal-title":"J. Food Meas Charact."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2900","DOI":"10.1021\/jf026187q","article-title":"Hierarchical Scheme for LC-MSn Identification of Chlorogenic Acids","volume":"51","author":"Clifford","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1002\/jsfa.8106","article-title":"characterization of autochthonous sweet cherry cultivars (Prunus Avium L.) of Southern Italy for fruit quality, bioactive compounds and antioxidant activity","volume":"97","author":"Matteo","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.foodchem.2017.01.154","article-title":"Comparison of Old Cherry Cultivars Grown in Czech Republic by Chemical Composition and Bioactive Compounds","volume":"228","author":"Ticha","year":"2017","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s13065-014-0066-z","article-title":"analyses of chlorogenic acids and related cinnamic acid derivatives from nicotiana tabacum tissues with the Aid of UPLC-QTOF-MS\/MS Based on the In-Source Collision-Induced Dissociation Method","volume":"8","author":"Ncube","year":"2014","journal-title":"Chem. Cent. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.foodres.2014.06.020","article-title":"Chemical composition, nutritional value and antioxidant properties of autochthonous Prunus avium cultivars from Campania Region","volume":"64","author":"Pacifico","year":"2014","journal-title":"Food Res. Int."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5762","DOI":"10.3390\/ijms16035762","article-title":"Phenolic composition and antioxidant properties of different peach [Prunus persica (L.) Batsch] Cultivars in China","volume":"16","author":"Zhao","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.supflu.2010.06.005","article-title":"Processing cherries (Prunus avium) using supercritical fluid technology. Part 1: Recovery of extract fractions rich in bioactive compounds","volume":"55","author":"Serra","year":"2010","journal-title":"J. Supercrit. Fluids."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0304-4238(87)90085-9","article-title":"Ageing-dependent responses of phloem flavonoids of Prunus avium graftings: Flavanone-, flavone- and isoflavone-glucosides","volume":"32","author":"Treutter","year":"1987","journal-title":"Sci. Hortic."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1021\/ja01564a056","article-title":"Flavonoids of Various Prunus Species. VI. The Flavonoids in the Wood of Prunus aequinoctialis, P. nipponica, P. Maximowiczii and P. avium","volume":"79","author":"Hasegawa","year":"1957","journal-title":"J. Am. Chem. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/0031-9422(90)85467-T","article-title":"Tectochrysin 5- and genistein 5-glucosides from the bark of Prunus cerasus","volume":"29","author":"Geibel","year":"1990","journal-title":"Phytochemistry"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1021\/jf980936f","article-title":"Antioxidant Polyphenols from Tart Cherries (Prunus cerasus)","volume":"47","author":"Wang","year":"1999","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.foodres.2012.11.022","article-title":"Antioxidant activity and polyphenol content of cherry stem (Cerasus avium L.) determined by LC\u2013MS\/MS","volume":"51","author":"Bursal","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"715","DOI":"10.5897\/JMPR2013.5350","article-title":"DNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cherry (Prunus avium L)","volume":"8","author":"Kutlu","year":"2014","journal-title":"J. Med. Plants Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.freeradbiomed.2004.04.034","article-title":"Potential toxicity of flavonoids and other dietary phenolics: Significance for their chemopreventive and anticancer properties","volume":"37","author":"Galati","year":"2004","journal-title":"Free Radic. Biol. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jfda.2017.02.002","article-title":"Antioxidant activity and anticancer effect of ethanolic and aqueous extracts of the roots of Ficus beecheyana and their phenolic components","volume":"26","author":"Yen","year":"2018","journal-title":"J. Food Drug. Anal."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.taap.2006.10.004","article-title":"In Vitro toxicity of silica nanoparticles in human lung cancer cells","volume":"217","author":"Lin","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/6\/1185\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:07:11Z","timestamp":1760162831000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/6\/1185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,25]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["foods10061185"],"URL":"https:\/\/doi.org\/10.3390\/foods10061185","relation":{"is-referenced-by":[{"id-type":"doi","id":"10.1007\/s11101-025-10079-9","asserted-by":"object"}]},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,25]]}}}