{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:42:15Z","timestamp":1762508535660,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,19]],"date-time":"2021-07-19T00:00:00Z","timestamp":1626652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/AGR\/04129\/2020","SFRH\/BD\/132832\/2017","PTDC\/BAA-AGR\/28608\/2017"],"award-info":[{"award-number":["UID\/AGR\/04129\/2020","SFRH\/BD\/132832\/2017","PTDC\/BAA-AGR\/28608\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>One of the most challenging problems with food-borne bioactive compounds is that there are commonly no cost-effective, generally recognized as safe (GRAS) methods for obtaining gram quantities of their purified forms. Here we aimed at developing a method to isolate deflamin, an oligomeric protein from lupin seeds with anti-inflammatory and anticancer activity through matrix metalloprotease (MMP)-9 inhibition. Our goal was to develop a GRAS method that could be easily up-scalable whilst maintaining deflamin\u2019s activity. A sequential precipitation methodology was developed, using an aqueous extraction, followed by heat denaturation, acid precipitation and solubilization in ethanol. A final precipitation with 90% ethanol yielded a purified protein which was sequenced through mass spectrometry and tested for its MMP inhibitory activity using the Dye-quenched (DQ) gelatin assay and the standard wound healing assay in HT29 cells. The developed method yielded a purified oligomer, which represented 0.1% (w\/w) of total dry seed weight and was positively confirmed to be deflamin. It further showed to effectively reduce MMP-9 gelatinolytic activity as well as colon cancer cell migration, hence corroborating the effectiveness of our method. Overall, this is the first reported method for isolating an MMP-9 inhibitor from legume seeds, which is up-scalable to an industrial level, in a cost-effective manner.<\/jats:p>","DOI":"10.3390\/foods10071663","type":"journal-article","created":{"date-parts":[[2021,7,19]],"date-time":"2021-07-19T21:39:15Z","timestamp":1626730755000},"page":"1663","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An Up-Scalable and Cost-Effective Methodology for Isolating a Polypeptide Matrix Metalloproteinase-9 Inhibitor from Lupinus albus Seeds"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8835-852X","authenticated-orcid":false,"given":"Joana","family":"Mota","sequence":"first","affiliation":[{"name":"LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1561-6858","authenticated-orcid":false,"given":"Maria E.","family":"Figueira","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5027-7564","authenticated-orcid":false,"given":"Ricardo B.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"given":"Ana","family":"Lima","sequence":"additional","affiliation":[{"name":"LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"},{"name":"Faculty of Veterinary Medicine, Universidade Lus\u00f3fona de Humanidades e Tecnologias, Campo Grande, 376, 1749-024 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,19]]},"reference":[{"key":"ref_1","first-page":"611","article-title":"The role of functional foods, nutraceuticals, and food supplements in intestinal health","volume":"2","author":"Cencic","year":"2010","journal-title":"Nutrition"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11767","DOI":"10.3748\/wjg.v21.i41.11767","article-title":"Colorectal cancer: Metastases to a single organ","volume":"21","author":"Vatandoust","year":"2015","journal-title":"World J. 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