{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T05:41:14Z","timestamp":1778218874469,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T00:00:00Z","timestamp":1637280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Worldwide, the food industry generates a large number of by-products from a wide variety of sources. These by-products represent an interesting and economical source of added value components with potential functionalities and\/or bioactivities, which might be explored for industrial purposes, encouraging and promoting the circular economy concept. In this context, the current work aimed to evaluate the fatty acids (FAs) profile using gas chromatography\u2013flame ionization detector (GC\u2013FID) and Fourier Transform Infrared (FTIR), as well as the determination of related health lipid indices (e.g., atherogenic (AI) and thrombogenic (TI)) as a powerful strategy to investigate the potential applications of different agri-food by-products for human nutrition and animal feeding. This work results showed that polyunsaturated fatty acids (PUFAs) are the predominant group in grape pomace (72.7%), grape bunches (54.3%), and brewer\u2019s spent grain (BSG, 59.0%), whereas carrot peels are dominated by monounsaturated fatty acids (MUFAs, 47.3%), and grape stems (46.2%), lees (from 50.8 to 74.1%), and potato peels (77.2%) by saturated fatty acids (SFAs). These findings represent a scientific basis for exploring the nutritional properties of agri-food by-products. Special attention should be given to grape pomace, grape bunches, and BSG since they have a high content of PUFAs (from 54.3 to 72.7%) and lower AI (from 0.11 to 0.38) and TI (from 0.30 to 0.56) indexes, suggesting their potential to provide a variety of health benefits against cardiovascular diseases including well-established hypotriglyceridemia and anti-inflammatory effects, products to which they are added.<\/jats:p>","DOI":"10.3390\/foods10112867","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T02:43:09Z","timestamp":1637289789000},"page":"2867","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Evaluation of Fatty Acids Profile as a Useful Tool towards Valorization of By-Products of Agri-Food Industry"],"prefix":"10.3390","volume":"10","author":[{"given":"Rui","family":"Ferreira","sequence":"first","affiliation":[{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-0894","authenticated-orcid":false,"given":"S\u00edlvia","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Polit\u00e9cnico de Leiria, Av. do Porto de Pesca, 2520-641 Peniche, Portugal"},{"name":"CIIMAR\/CIMAR\u2014Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Andr\u00e9","family":"Lopes","sequence":"additional","affiliation":[{"name":"OOM\u2014Observat\u00f3rio Oce\u00e2nico da Madeira, Edif\u00edcio Madeira Tecnopolo, Piso 0, Caminho da Penteada, 9020-105 Funchal, Portugal"},{"name":"CCMAR\u2014Centro de Ci\u00eancias do Mar, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8166-0013","authenticated-orcid":false,"given":"Carlos","family":"Andrade","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"OOM\u2014Observat\u00f3rio Oce\u00e2nico da Madeira, Edif\u00edcio Madeira Tecnopolo, Piso 0, Caminho da Penteada, 9020-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1965-3151","authenticated-orcid":false,"given":"Jos\u00e9 S.","family":"C\u00e2mara","sequence":"additional","affiliation":[{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"},{"name":"Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias Exatas e Engenharia, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8303-4286","authenticated-orcid":false,"given":"Paula","family":"Castilho","sequence":"additional","affiliation":[{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7223-1022","authenticated-orcid":false,"given":"Rosa","family":"Perestrelo","sequence":"additional","affiliation":[{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1039\/c2gc36978h","article-title":"Food waste biomass: A resource for high-value chemicals","volume":"15","author":"Pfaltzgraff","year":"2013","journal-title":"Green Chem."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Chetrariu, A., and Dabija, A. (2020). Brewer\u2019s Spent Grains: Possibilities of Valorization, a Review. Appl. Sci., 10.","DOI":"10.3390\/app10165619"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1080\/10643389.2019.1694819","article-title":"Valorization of selected fruit and vegetable wastes as bioactive compounds: Opportunities and challenges","volume":"50","author":"Esparza","year":"2020","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Faustino, A.M., Veiga, M., Sousa, P., Costa, E.M., Silva, S., and Pintado, M. (2019). Agro-Food Byproducts as a New Source of Natural Food Additives. Molecules, 24.","DOI":"10.3390\/molecules24061056"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"104758","DOI":"10.1016\/j.microc.2020.104758","article-title":"Exploring the potential of wine industry by-products as source of additives to improve the quality of aquafeed","volume":"155","author":"Silva","year":"2020","journal-title":"Microchem. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1002\/jssc.202100108","article-title":"Identification of high-value generating molecules from the wastes of tuna fishery industry by liquid chromatography and gas chromatography hyphenated techniques with automated sample preparation","volume":"44","author":"Rigano","year":"2021","journal-title":"J. Sep. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"105028","DOI":"10.1016\/j.resconrec.2020.105028","article-title":"Indicators for circular economy in the agri-food sector","volume":"163","author":"Mendoza","year":"2020","journal-title":"Resour. Conserv. Recycl."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Fraga-Corral, M., Otero, P., Echave, J., Garcia-Oliveira, P., Carpena, M., Jarboui, A., Nu\u00f1ez-Estevez, B., Simal-Gandara, J., and Prieto, M. (2021). By-Products of Agri-Food Industry as Tannin-Rich Sources: A Review of Tannins\u2019 Biological Activities and Their Potential for Valorization. Foods, 10.","DOI":"10.3390\/foods10010137"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8987","DOI":"10.1021\/jf402586f","article-title":"Grape Pomace as a Sustainable Source of Bioactive Compounds: Extraction, Characterization, and Biotechnological Applications of Phenolics","volume":"61","author":"Fontana","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Lucarini, M., Durazzo, A., Kiefer, J., Santini, A., Lombardi-Boccia, G., Souto, E., Romani, A., Lampe, A., Nicoli, S.F., and Gabrielli, P. (2019). Grape Seeds: Chromatographic Profile of Fatty Acids and Phenolic Compounds and Qualitative Analysis by FTIR-ATR Spectroscopy. Foods, 9.","DOI":"10.3390\/foods9010010"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1002\/ptr.6419","article-title":"Polyphenols: A concise overview on the chemistry, occurrence, and human health","volume":"33","author":"Durazzo","year":"2019","journal-title":"Phytother. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ferhi, S., Santaniello, S., Zerizer, S., Cruciani, S., Fadda, A., Sanna, D., Dore, A., Maioli, M., and D\u2019Hallewin, G. (2019). Total Phenols from Grape Leaves Counteract Cell Proliferation and Modulate Apoptosis-Related Gene Expression in MCF-7 and HepG2 Human Cancer Cell Lines. Molecules, 24.","DOI":"10.3390\/molecules24030612"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"103515","DOI":"10.1016\/j.jff.2019.103515","article-title":"Anti-cancer activity of grape seed semi-polar extracts in human mesothelioma cell lines","volume":"61","author":"Aversano","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bijak, M., Sut, A., Kosiorek, A., Saluk-Bijak, J., and Golanski, J. (2019). Dual Anticoagulant\/Antiplatelet Activity of Polyphenolic Grape Seeds Extract. Nutrients, 11.","DOI":"10.3390\/nu11010093"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.foodchem.2012.09.148","article-title":"Wine grape pomace as antioxidant dietary fibre for enhancing nutritional value and improving storability of yogurt and salad dressing","volume":"138","author":"Tseng","year":"2013","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Iriondo-DeHond, M., Miguel, E., and Del Castillo, M.D. (2018). Food Byproducts as Sustainable Ingredients for Innovative and Healthy Dairy Foods. Nutrients, 10.","DOI":"10.3390\/nu10101358"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1002\/jsfa.6486","article-title":"Brewer\u2019s spent grain: A valuable feedstock for industrial applications","volume":"94","author":"Mussatto","year":"2014","journal-title":"J. Sci. Food Agric."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Karlovi\u0107, A., Juri\u0107, A., \u0106ori\u0107, N., Habschied, K., Krstanovi\u0107, V., and Mastanjevi\u0107, K. (2020). By-Products in the Malting and Brewing Industries\u2014Re-Usage Possibilities. Fermentation, 6.","DOI":"10.3390\/fermentation6030082"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"103294","DOI":"10.1016\/j.jfca.2019.103294","article-title":"Fatty acid profile of fruits (pulp and peel) and cladodes (young and old) of prickly pear [Opuntia ficus-indica (L.) Mill.] from six Spanish cultivars","volume":"84","author":"Sendra","year":"2019","journal-title":"J. Food Compos. Anal."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s40169-017-0153-6","article-title":"Significance of long chain polyunsaturated fatty acids in human health","volume":"6","author":"Tejera","year":"2017","journal-title":"Clin. Transl. Med."},{"key":"ref_21","first-page":"427","article-title":"A review on role of fish in human nutrition with special emphasis to essential fatty acid","volume":"6","author":"Pandey","year":"2018","journal-title":"Int. J. Fish. Aquat. Stud."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Nguyen, Q.V., Malau-Aduli, B.S., Cavalieri, J., Malau-Aduli, A.E.O., and Nichols, P.D. (2019). Enhancing Omega-3 Long-Chain Polyunsaturated Fatty Acid Content of Dairy-Derived Foods for Human Consumption. Nutrients, 11.","DOI":"10.3390\/nu11040743"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1016\/j.psj.2019.10.026","article-title":"Fatty acid profiles and health lipid indices in the breast muscles of local Polish goose varieties","volume":"99","author":"Haraf","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Jarukas, L., Kuraite, G., Baranauskaite, J., Marksa, M., Bezruk, I., and Ivanauskas, L. (2020). Optimization and Validation of the GC\/FID Method for the Quantification of Fatty Acids in Bee Products. Appl. Sci., 11.","DOI":"10.3390\/app11010083"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jchromb.2015.11.005","article-title":"Profile of bioactive compounds from grape pomace (Vitis vinifera and Vitis labrusca) by spectrophotometric, chromatographic and spectral analyses","volume":"1007","author":"Ribeiro","year":"2015","journal-title":"J. Chromatogr. B"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"269","DOI":"10.4025\/actascitechnol.v39i3.28435","article-title":"Bioactive compounds from brewer\u2019s spent grain: Phenolic compounds, fatty acids and in vitro antioxidant capacity","volume":"39","author":"Almeida","year":"2017","journal-title":"Acta Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9910","DOI":"10.1021\/jf302684x","article-title":"Characterization of Lipids and Lignans in Brewer\u2019s Spent Grain and Its Enzymatically Extracted Fraction","volume":"60","author":"Niemi","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"914693","DOI":"10.1155\/2012\/914693","article-title":"Supercritical SC-CO2 and soxhlet n-hexane extract of Tunisian Opuntia ficus indica seeds and fatty acids analysis","volume":"2012","author":"Yeddes","year":"2012","journal-title":"J. Lipids"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Cer\u00f3n-Mart\u00ednez, L., Hurtado-Benavides, A., Ayala-Aponte, A., Serna-Cock, L., and Tirado, D. (2021). A Pilot-Scale Supercritical Carbon Dioxide Extraction to Valorize Colombian Mango Seed Kernel. Molecules, 26.","DOI":"10.3390\/molecules26082279"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Alinafiah, S.M., Azlan, A., Ismail, A., and Ab Rashid, N.-K.M. (2021). Method Development and Validation for Omega-3 Fatty Acids (DHA and EPA) in Fish Using Gas Chromatography with Flame Ionization Detection (GC-FID). Molecules, 26.","DOI":"10.3390\/molecules26216592"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"78","DOI":"10.15414\/afz.2020.23.02.78-84","article-title":"Fatty acid profile analysis of grape by-products from Slovakia and Austria","volume":"23","author":"Simko","year":"2020","journal-title":"Acta Fytotech. Zootech."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jpha.2015.01.005","article-title":"Development and validation of a GC\u2013FID method for quantitative analysis of oleic acid and related fatty acids","volume":"5","author":"Zhang","year":"2015","journal-title":"J. Pharm. Anal."},{"key":"ref_33","unstructured":"FAO (2020). The State of World Fisheries and Aquaculture 2020. Sustainability in Action, FAO."},{"key":"ref_34","first-page":"3087","article-title":"Alternative feed ingredients in aquaculture: Opportunities and challenges","volume":"6","author":"Bandara","year":"2018","journal-title":"J. Entomol. Zool. Stud."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1080\/713610925","article-title":"Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish","volume":"11","author":"Tocher","year":"2003","journal-title":"Rev. Fish. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1007\/s12562-018-1254-x","article-title":"Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: A comprehensive review","volume":"84","author":"Monroig","year":"2018","journal-title":"Fish. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jcs.2015.04.003","article-title":"Volatile profile, fatty acids composition and total phenolics content of brewers\u2019 spent grain by-product with potential use in the development of new functional foods","volume":"64","author":"Socaci","year":"2015","journal-title":"J. Cereal Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Sancho-Gal\u00e1n, P., Amores-Arrocha, A., Jim\u00e9nez-Cantizano, A., and Palacios, V. (2020). Physicochemical and Nutritional Characterization of Winemaking Lees: A New Food Ingredient. Agronomy, 10.","DOI":"10.3390\/agronomy10070996"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s11540-019-9419-2","article-title":"Potato Peel as a Sustainable Resource of Natural Antioxidants for the Food Industry","volume":"62","author":"Samotyja","year":"2019","journal-title":"Potato Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/0140-6736(91)91846-M","article-title":"Coronary heart disease: Seven dietary factors","volume":"338","author":"Ulbricht","year":"1991","journal-title":"Lancet"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jfca.2010.07.011","article-title":"Fatty acid profiles of livers from selected marine fish species","volume":"24","year":"2011","journal-title":"J. Food Compos. Anal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1023\/A:1008812904017","article-title":"Rapid simultaneous lipid extraction and transesterification for fatty acid analyses","volume":"12","author":"Belarbi","year":"1998","journal-title":"Biotechnol. Tech."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Dimi\u0107, I., Tesli\u0107, N., Putnik, P., Bursa\u0107 Kova\u010devi\u0107, D., Zekovi\u0107, Z., \u0160oji\u0107, B., Mrkonji\u0107, \u017d., \u010colovi\u0107, D., Montesano, D., and Pavli\u0107, B. (2020). Innovative and conventional valorizations of grape seeds from winery by-products as sustainable source of lipophilic antioxidants. Antioxidants, 9.","DOI":"10.3390\/antiox9070568"},{"key":"ref_44","first-page":"92","article-title":"Multivariate Analysis in Metabolomics","volume":"1","author":"Worley","year":"2013","journal-title":"Curr. Metabolomics"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Laroche, M., Perreault, V., Marciniak, A., Gravel, A., Chamberland, J., and Doyen, A. (2019). Comparison of Conventional and Sustainable Lipid Extraction Methods for the Production of Oil and Protein Isolate from Edible Insect Meal. Foods, 8.","DOI":"10.3390\/foods8110572"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1007\/s00011-019-01273-5","article-title":"The effect of palmitic acid on inflammatory response in macrophages: An overview of molecular mechanisms","volume":"68","author":"Korbecki","year":"2019","journal-title":"Inflamm. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1071\/AN16495","article-title":"In vitro evaluation of the methane mitigation potential of a range of grape marc products","volume":"57","author":"Russo","year":"2017","journal-title":"Anim. Prod. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.foodchem.2015.04.140","article-title":"Meat composition, fatty acid profile and oxidative stability of meat from broilers supplemented with pomegranate (Punica granatum L.) by-products","volume":"188","author":"Ahmed","year":"2015","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Simopoulos, A.P. (2016). An Increase in the Omega-6\/Omega-3 Fatty Acid Ratio Increases the Risk for Obesity. Nutrients, 8.","DOI":"10.3390\/nu8030128"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.jfca.2008.09.003","article-title":"Application of NIR and MIR spectroscopy in quality control of potato chips","volume":"22","author":"Shiroma","year":"2009","journal-title":"J. Food Compos. Anal."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Moreira, J.L., and Santos, L. (2004). Spectroscopic Interferences in Fourier Transform Infrared Wine Analysis, Elsevier.","DOI":"10.1016\/j.aca.2003.09.029"}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/11\/2867\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:32:34Z","timestamp":1760167954000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/11\/2867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,19]]},"references-count":51,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["foods10112867"],"URL":"https:\/\/doi.org\/10.3390\/foods10112867","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,19]]}}}