{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T12:54:11Z","timestamp":1768827251104,"version":"3.49.0"},"reference-count":63,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Ci\u00eancia e Tecnologia","award":["PTDC\/BIA-BQM\/28355\/2017"],"award-info":[{"award-number":["PTDC\/BIA-BQM\/28355\/2017"]}]},{"name":"Funda\u00e7\u00e3o Ci\u00eancia e Tecnologia","award":["UIDB\/04292\/2020 (MARE) and UID\/MULTI\/04046\/2019 ("],"award-info":[{"award-number":["UIDB\/04292\/2020 (MARE) and UID\/MULTI\/04046\/2019 ("]}]},{"name":"Funda\u00e7\u00e3o Ci\u00eancia e Tecnologia","award":["PD65\u20132012 (PD\/BD\/142861\/2018)"],"award-info":[{"award-number":["PD65\u20132012 (PD\/BD\/142861\/2018)"]}]},{"name":"Funda\u00e7\u00e3o Ci\u00eancia e Tecnologia","award":["(CEECIND\/00511\/2017)"],"award-info":[{"award-number":["(CEECIND\/00511\/2017)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Fucus vesiculosus L. is a common coastal brown seaweed associated with various benefits to human health due to its phenolic content and nutrients and is used as food through different methods of consumption. This study aims to evaluate the influence of the seaweed\u2019s gender and growth stage on different types of biological activities as well as its chemical constitution and elements present. Akin to food preparation, aqueous extracts of the seaweed were prepared at 25 \u00b0C (salad) and 100 \u00b0C (soup). Biological activities were determined by measuring total phenol content (TPC), antioxidant activity and inhibition of acetylcholinesterase (AChE). Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS\/MS) was used for compound identification, and elemental analysis was carried out by using Total Reflection X-ray Fluorescence Spectrometry (TXRF). Older females and males had higher TPC compared to the new ones at 100 \u00b0C. Antioxidant activity depended on the extraction temperature but was higher for the youngest male at 100 \u00b0C. AChE inhibitory activity was higher for older males at 25 \u00b0C, but at 100 \u00b0C it was higher for older females. Primary metabolites and various phloroglucinol were the main compounds identified. Additionally, since this seaweed is often harvested in estuarine systems with high anthropogenic impacts, its safety was evaluated through the evaluation of the sample\u2019s metal content. The heavy metals detected are within the limits established by various regulating entities, pointing to a safe food source.<\/jats:p>","DOI":"10.3390\/foods11010039","type":"journal-article","created":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T08:38:46Z","timestamp":1640335126000},"page":"39","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Influence of Gender and Age of Brown Seaweed (Fucus vesiculosus) on Biochemical Activities of Its Aqueous Extracts"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0793-8750","authenticated-orcid":false,"given":"Diogo","family":"Nunes","sequence":"first","affiliation":[{"name":"BioISI\u2014Biosystems & Integrative Sciences Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2733-5797","authenticated-orcid":false,"given":"Rebeca","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"BioISI\u2014Biosystems & Integrative Sciences Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5525-103X","authenticated-orcid":false,"given":"Asma","family":"Ressaissi","sequence":"additional","affiliation":[{"name":"BioISI\u2014Biosystems & Integrative Sciences Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1914-7435","authenticated-orcid":false,"given":"Bernardo","family":"Duarte","sequence":"additional","affiliation":[{"name":"Marine and Environmental Sciences Centre (MARE), Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6995-2630","authenticated-orcid":false,"given":"Ricardo","family":"Melo","sequence":"additional","affiliation":[{"name":"Marine and Environmental Sciences Centre (MARE), Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7541-9613","authenticated-orcid":false,"given":"Maria Lu\u00edsa","family":"Serralheiro","sequence":"additional","affiliation":[{"name":"BioISI\u2014Biosystems & Integrative Sciences Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Department of Chemistry and Biochemistry, Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, C8 bldg, 1749-016 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1089\/acm.1995.1.131","article-title":"The Role of Plants in Traditional Medicine","volume":"1","author":"Houghton","year":"1995","journal-title":"J. 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