{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:54:54Z","timestamp":1773888894221,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project PROMETHEUS-POCI-01-0145-FEDER-029284","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"Project PROMETHEUS-POCI-01-0145-FEDER-029284","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}]},{"name":"Project PROMETHEUS-POCI-01-0145-FEDER-029284","award":["UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDP\/00511\/2020"]}]},{"name":"Project PROMETHEUS-POCI-01-0145-FEDER-029284","award":["PROMETHEUS-POCI-01-0145-FEDER-029284"],"award-info":[{"award-number":["PROMETHEUS-POCI-01-0145-FEDER-029284"]}]},{"name":"FEDER funds through COMPETE2020\u2014Programa Operacional Competitividade e Internacionaliza\u00e7\u00e3o (POCI)","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"FEDER funds through COMPETE2020\u2014Programa Operacional Competitividade e Internacionaliza\u00e7\u00e3o (POCI)","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}]},{"name":"FEDER funds through COMPETE2020\u2014Programa Operacional Competitividade e Internacionaliza\u00e7\u00e3o (POCI)","award":["UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDP\/00511\/2020"]}]},{"name":"FEDER funds through COMPETE2020\u2014Programa Operacional Competitividade e Internacionaliza\u00e7\u00e3o (POCI)","award":["PROMETHEUS-POCI-01-0145-FEDER-029284"],"award-info":[{"award-number":["PROMETHEUS-POCI-01-0145-FEDER-029284"]}]},{"name":"national funds (PIDDAC) through FCT\/MCTES","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"national funds (PIDDAC) through FCT\/MCTES","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}]},{"name":"national funds (PIDDAC) through FCT\/MCTES","award":["UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDP\/00511\/2020"]}]},{"name":"national funds (PIDDAC) through FCT\/MCTES","award":["PROMETHEUS-POCI-01-0145-FEDER-029284"],"award-info":[{"award-number":["PROMETHEUS-POCI-01-0145-FEDER-029284"]}]},{"name":"ALiCE","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"ALiCE","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}]},{"name":"ALiCE","award":["UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDP\/00511\/2020"]}]},{"name":"ALiCE","award":["PROMETHEUS-POCI-01-0145-FEDER-029284"],"award-info":[{"award-number":["PROMETHEUS-POCI-01-0145-FEDER-029284"]}]},{"name":"LEPABE","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"LEPABE","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}]},{"name":"LEPABE","award":["UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDP\/00511\/2020"]}]},{"name":"LEPABE","award":["PROMETHEUS-POCI-01-0145-FEDER-029284"],"award-info":[{"award-number":["PROMETHEUS-POCI-01-0145-FEDER-029284"]}]},{"name":"national funds through FCT\/MCTES (PIDDAC)","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"national funds through FCT\/MCTES (PIDDAC)","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}]},{"name":"national funds through FCT\/MCTES (PIDDAC)","award":["UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDP\/00511\/2020"]}]},{"name":"national funds through FCT\/MCTES (PIDDAC)","award":["PROMETHEUS-POCI-01-0145-FEDER-029284"],"award-info":[{"award-number":["PROMETHEUS-POCI-01-0145-FEDER-029284"]}]},{"name":"Partnership Agreement with Faculty of Engineering of University of Porto","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"Partnership Agreement with Faculty of Engineering of University of Porto","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}]},{"name":"Partnership Agreement with Faculty of Engineering of University of Porto","award":["UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDP\/00511\/2020"]}]},{"name":"Partnership Agreement with Faculty of Engineering of University of Porto","award":["PROMETHEUS-POCI-01-0145-FEDER-029284"],"award-info":[{"award-number":["PROMETHEUS-POCI-01-0145-FEDER-029284"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Current market trends point at increasing demand for functional foods, namely those carrying probiotics. In the case of table olives, presence of probiotics would convey a competitive advantage to Mediterranean-based diets, already established for their cultural heritage and gastronomic character. This work assessed the safety and resistance to gastrointestinal digestion of 19 native LAB strains from Cobran\u00e7osa table olives. Strains were identified via molecular sequencing (4 fingerprints\/10 strains for Lactiplantibacillus\u00a0pentosus, and 2 fingerprints\/9 strains for L. paraplantarum), and exposed to simulated gastrointestinal fluids, as per the INFOGEST in vitro protocol with modifications. None of those strains proved dangerous for human consumption. Survivability to the gastrointestinal resistance test ranged from 29% to 70%, with strain-dependent variability. L. paraplantarum i18, i27, and i102, and L. pentosus i10 and i11 exhibited statistically lower survival rates (29\u201335%) than probiotic the Greek table olive reference strain L. pentosus B281 (53%). Among the other strains, L. paraplantarum i101 and L. pentosus i53 and i106 showed the highest survival rates but were not significantly different from the strain of Lacticaseibacillus casei isolated from commercial probiotic yoghurt (65\u201370%). In vitro results proved that strains retrieved from fermenting cultivar Cobran\u00e7osa possess the potential to be claimed as probiotics\u2014thus deserving further attention toward the development of a specific starter culture.<\/jats:p>","DOI":"10.3390\/foods11193050","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T04:04:56Z","timestamp":1665201896000},"page":"3050","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Study of Lactic Acid Bacteria Biodiversity in Fermented Cobran\u00e7osa Table Olives to Determine Their Probiotic Potential"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1234-2713","authenticated-orcid":false,"given":"Joana","family":"Coimbra-Gomes","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8106-8474","authenticated-orcid":false,"given":"Patr\u00edcia J. M.","family":"Reis","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"T\u00e2nia G.","family":"Tavares","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Francisco Xavier","family":"Malcata","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6854-9855","authenticated-orcid":false,"given":"Angela C.","family":"Macedo","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Doutor Roberto Frias, 4200-465 Porto, Portugal"},{"name":"UNICES\u2014Research Unit in Management Sciences and Sustainability, University of Maia, Avenida Carlos de Oliveira Campos, 4475-690 Maia, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1108\/EJIM-05-2021-0258","article-title":"A review of the literature on innovation in the agrofood industry: Sustainability, smartness and health","volume":"25","author":"Bigliardi","year":"2022","journal-title":"Eur. J. Innov. Manag."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100013","DOI":"10.1016\/j.fufo.2021.100013","article-title":"Probiotics-based foods and beverages as future foods and their overall safety and regulatory claims","volume":"3","author":"Koirala","year":"2021","journal-title":"Future Foods"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1800129","DOI":"10.1002\/ejlt.201800129","article-title":"An overview of Portuguese olive oils and table olives with Protected Designation of Origin","volume":"121","author":"Albuquerque","year":"2019","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"104821","DOI":"10.1016\/j.jff.2021.104821","article-title":"Probiotic bacteria and plant-based matrices: An association with improved health-promoting features","volume":"87","author":"Sisto","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1108\/BFJ-03-2021-0225","article-title":"Worldwide research tendencies on probiotics in food science: 1993 to 2021","volume":"124","author":"Duran","year":"2022","journal-title":"Br. Food J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"797295","DOI":"10.3389\/fmicb.2021.797295","article-title":"Current status, recent advances, and main challenges on table olive fermentation: The present meets the future","volume":"12","author":"Anagnostopoulos","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Perpetuini, G., Prete, R., Garcia-Gonzalez, N., Khairul Alam, M., and Corsetti, A. (2020). Table olives more than a fermented food. Foods, 9.","DOI":"10.3390\/foods9020178"},{"key":"ref_8","first-page":"611","article-title":"Physicochemical, nutritional and microbiological characteristics of traditional table olives from Southern Portugal","volume":"30","author":"Nunes","year":"2018","journal-title":"Emir. J. Food Agric."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.lwt.2014.03.003","article-title":"Novel isolates of lactobacilli from fermented Portuguese olive as potential probiotics","volume":"59","author":"Peres","year":"2014","journal-title":"LWT Food Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.ifset.2017.06.003","article-title":"Probiotic potential of indigenous yeasts isolated during the fermentation of table olives from Northeast of Portugal","volume":"44","author":"Oliveira","year":"2017","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.fm.2014.10.003","article-title":"YEAST dynamics during the natural fermentation process of table olives (Negrinha de Freixo cv.)","volume":"46","author":"Pereira","year":"2015","journal-title":"Food Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1007\/s13213-018-1386-3","article-title":"Screening for potential probiotic from spontaneously fermented non-dairy foods based on in vitro probiotic and safety properties","volume":"68","author":"Wang","year":"2018","journal-title":"Ann. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1007\/s12602-019-09543-8","article-title":"Characterization of Probiotic Properties of Antifungal Lactobacillus Strains Isolated from Traditional Fermenting Green Olives","volume":"12","author":"Abouloifa","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1016\/j.biotechadv.2018.09.003","article-title":"How to select a probiotic? A review and update of methods and criteria","volume":"36","author":"Soccol","year":"2018","journal-title":"Biotechnol. Adv."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Bonatsou, S., Tassou, C.C., Panagou, E.Z., and Nychas, G.E. (2017). Table olive fermentation using starter cultures with multifunctional potential. Microorganisms, 5.","DOI":"10.3390\/microorganisms5020030"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.fm.2012.08.009","article-title":"Induction of bacteriocin production by coculture is widespread among plantaricin-producing Lactobacillus plantarum strains with different regulatory operons","volume":"33","year":"2013","journal-title":"Food Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1099\/ijsem.0.001755","article-title":"Introducing EzBioCloud: A taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies","volume":"67","author":"Yoon","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"836","DOI":"10.3389\/fmicb.2019.00836","article-title":"Biodiversity and multifunctional features of lactic acid bacteria isolated from table olive biofilms","volume":"10","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Anagnostopoulos, D., Bozoudi, D., and Tsaltas, D. (2018). Enterococci isolated from Cypriot green table olives as a new source of technological and probiotic properties. Fermentation, 4.","DOI":"10.3390\/fermentation4020048"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised static in vitro digestion method suitable for food\u2014An international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1039\/C9FO01293A","article-title":"A standardised semi-dynamic in vitro digestion method suitable for food\u2014An international consensus","volume":"11","author":"Egger","year":"2020","journal-title":"Food Funct."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.foodres.2012.10.004","article-title":"Screening of lactic acid bacteria isolated from fermented table olives with probiotic potential","volume":"50","author":"Rantsiou","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2134\/agronj2017.10.0580","article-title":"Letters in mean comparisons: What they do and don\u2019t mean","volume":"110","author":"Piepho","year":"2018","journal-title":"J. Agron."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Portilha-Cunha, M.F., Macedo, A.C., and Malcata, F.X. (2020). A review on adventitious lactic acid bacteria from table olives. Foods, 9.","DOI":"10.3390\/foods9070948"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108542","DOI":"10.1016\/j.foodres.2019.108542","article-title":"The resistance of Bacillus, Bifidobacterium, and Lactobacillus strains with claimed probiotic properties in different food matrices exposed to simulated gastrointestinal tract conditions","volume":"125","author":"Soares","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Botta, C., Langerholc, T., Cencic, A., and Cocolin, L. (2014). In vitro selection and characterization of new probiotic candidates from table olive microbiota. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094457"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.3389\/fmicb.2016.01583","article-title":"Fermented Alorena table olives as a source of potential probiotic Lactobacillus pentosus strains","volume":"7","author":"Montoro","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_28","first-page":"540","article-title":"Survival of pathogenic and lactobacilli species of fermented olives during simulated human digestion","volume":"5","author":"Denis","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e595","DOI":"10.3389\/fmicb.2018.00595","article-title":"In vitro and in vivo selection of potentially probiotic Lactobacilli from Nocellara del Belice table olives","volume":"9","author":"Guantario","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.anaerobe.2013.06.006","article-title":"Identification and evaluation of the probiotic potential of Lactobacillus paraplantarum FT259, a bacteriocinogenic strain isolated from Brazilian semi-hard artisanal cheese","volume":"22","author":"Tulini","year":"2013","journal-title":"Annaerobe"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e14015","DOI":"10.1111\/jfpp.14015","article-title":"Probiotic potential of Lactobacillus paraplantarum BT-11 isolated from raw buffalo (Bubalus bubalis) milk and characterization of bacteriocin-like inhibitory substance produced","volume":"43","author":"Kalhoro","year":"2019","journal-title":"J. Food Process. Preserv."},{"key":"ref_32","unstructured":"IOOC (2022, May 25). IOC News: Table Olives\u2014Provisional 2019\/20 Campaign and Estimate for 2020\/21. Available online: https:\/\/www.internationaloliveoil.org\/."},{"key":"ref_33","unstructured":"IOOC (2022, May 25). IOC Figures: Production of Table Olives. Available online: https:\/\/www.internationaloliveoil.org\/."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1007\/s12602-019-09624-8","article-title":"Potentiated in vitro probiotic activities of Lactobacillus fermentum LfQi6 biofilm biomass versus planktonic culture","volume":"12","author":"Berkes","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/19\/3050\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:44:56Z","timestamp":1760143496000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/19\/3050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,1]]},"references-count":34,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["foods11193050"],"URL":"https:\/\/doi.org\/10.3390\/foods11193050","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,1]]}}}