{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T03:19:16Z","timestamp":1768619956501,"version":"3.49.0"},"reference-count":98,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T00:00:00Z","timestamp":1667001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Portuguese Foundation for Science and Technology","award":["UIDB\/04050\/2020"],"award-info":[{"award-number":["UIDB\/04050\/2020"]}]},{"name":"FCT\u2014Portuguese Foundation for Science and Technology","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}]},{"name":"FCT\u2014Portuguese Foundation for Science and Technology","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}]},{"name":"FCT\u2014Portuguese Foundation for Science and Technology","award":["PPBI-POCI-01-0145-FEDER-022122"],"award-info":[{"award-number":["PPBI-POCI-01-0145-FEDER-022122"]}]},{"name":"ICVS","award":["UIDB\/04050\/2020"],"award-info":[{"award-number":["UIDB\/04050\/2020"]}]},{"name":"ICVS","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}]},{"name":"ICVS","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}]},{"name":"ICVS","award":["PPBI-POCI-01-0145-FEDER-022122"],"award-info":[{"award-number":["PPBI-POCI-01-0145-FEDER-022122"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["UIDB\/04050\/2020"],"award-info":[{"award-number":["UIDB\/04050\/2020"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["PPBI-POCI-01-0145-FEDER-022122"],"award-info":[{"award-number":["PPBI-POCI-01-0145-FEDER-022122"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Osteoarthritis (OA), a progressive degenerative disease of weight-bearing joints, is the second leading cause of disability in the world. Despite all the advances and research over the last years, none of the proposed strategies has been effective in generating functional and long-lasting tissue. Due to the high prevalence of OA and the urgent need for an effective and successful treatment, interest in natural products as anti-inflammatory agents, such as propolis and its components, has emerged. In this work, we estimate the biomedical potential of Portuguese propolis, evaluating the in vitro antioxidant and anti-inflammatory effects of single hydroalcoholic extracts prepared with propolis from Ger\u00eas sampled over a five-year period (2011\u20132015) (G.EE70 and G.EE35). The in vivo and in vitro anti-inflammatory potential of the hydroalcoholic extract of mixtures of the same samples (mG.EE70 and mG.EE35) was evaluated for the first time too. DPPH\u2022 radical scavenging and superoxide anion scavenging assays showed the strong antioxidant potential of both hydroalcoholic extracts, either prepared from single propolis samples or from the mixtures of the same samples. Results also revealed an anti-inflammatory effect of mG.EE35, both in vitro by inhibiting BSA denaturation and in vivo in the OA-induced model by improving mechanical hyperalgesia as well as the gait pattern parameters. Results further support the use of propolis blends as a better and more efficient approach to take full advantage of the bioactive potential of propolis.<\/jats:p>","DOI":"10.3390\/foods11213431","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T04:57:34Z","timestamp":1667105854000},"page":"3431","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["An Insight on the Biomedical Potential of Portuguese Propolis from Ger\u00eas"],"prefix":"10.3390","volume":"11","author":[{"given":"Carina","family":"Ara\u00fajo","sequence":"first","affiliation":[{"name":"Biology Department, University of Minho, 4710-057 Braga, Portugal"},{"name":"Life and Health Sciences Research Institute (ICVS), University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9816-5919","authenticated-orcid":false,"given":"Rafaela Dias","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Biology Department, University of Minho, 4710-057 Braga, Portugal"},{"name":"Life and Health Sciences Research Institute (ICVS), University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4806-909 Guimar\u00e3es, Portugal"},{"name":"CBMA\u2014Centre of Molecular and Environmental Biology, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7574-6489","authenticated-orcid":false,"given":"Filipa","family":"Pinto-Ribeiro","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4806-909 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8223-8634","authenticated-orcid":false,"given":"Cristina","family":"Almeida-Aguiar","sequence":"additional","affiliation":[{"name":"Biology Department, University of Minho, 4710-057 Braga, Portugal"},{"name":"CBMA\u2014Centre of Molecular and Environmental Biology, University of Minho, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Arias, C., Saavedra, N., Saavedra, K., Alvear, M., Cuevas, A., Maria-Engler, S.S., Abdalla, D.S.P., and Salazar, L.A. 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