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In this study, for the first time, these microalgae were incorporated as fillers in starch-based films, envisioning the development of biodegradable and bioactive materials for food packaging applications. The films were obtained by solvent casting using different proportions of the filler (2.5, 5, 10, and 20%, w\/w). For comparison, commercial CaCO3, used as filler in the plastic industry, was also tested. The incorporation of CaCO3 and microalgae (EHUX or CP) made the films significantly less rigid, decreasing Young\u2019s modulus up to 4.7-fold. Moreover, the incorporation of microalgae hydrophobic compounds as lipids turned the surface hydrophobic (water contact angles &gt; 90\u00b0). Contrary to what was observed with commercial CaCO3, the films prepared with microalgae exhibited antioxidant activity, increasing from 0.9% (control) up to 60.4% (EHUX 20%) of ABTS radical inhibition. Overall, the introduction of microalgae biomass improved hydrophobicity and antioxidant capacity of starch-based films. These findings should be considered for further research using coccolithophores to produce active and sustainable food packaging material.<\/jats:p>","DOI":"10.3390\/foods12030513","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T03:48:08Z","timestamp":1674445688000},"page":"513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Potential of Coccolithophore Microalgae as Fillers in Starch-Based Films for Active and Sustainable Food Packaging"],"prefix":"10.3390","volume":"12","author":[{"given":"Ana S. P.","family":"Moreira","sequence":"first","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"},{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8648-9438","authenticated-orcid":false,"given":"Joana","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"given":"Francisco","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7509-2216","authenticated-orcid":false,"given":"In\u00eas","family":"Maia","sequence":"additional","affiliation":[{"name":"CCMAR\u2014Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Hugo","family":"Pereira","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Joana","family":"Silva","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8898-6342","authenticated-orcid":false,"given":"Manuel A.","family":"Coimbra","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6578-8164","authenticated-orcid":false,"given":"Paula","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7217-2790","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Nunes","sequence":"additional","affiliation":[{"name":"Department of Materials and Ceramic Engineering, CICECO, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125263","DOI":"10.1016\/j.jclepro.2020.125263","article-title":"A review on European Union\u2019s strategy for plastics in a circular economy and its impact on food safety","volume":"283","author":"Matthews","year":"2021","journal-title":"J. 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