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Current trends in citrus waste focus on the extraction of highly valued bioactive compounds via single-compound extraction. There is a lack of knowledge on how these methodologies can be introduced into extraction schemes of bioactive compounds, maximizing the residue potential and reducing its amount. The present work aimed to address this issue by designing a consecutive extraction of pectin and hesperidin from orange peel waste. A novel method for extraction and precipitation of hesperidin with an eco-friendly approach is also presented. After neutral pretreatment, pectin extraction was conducted under acidic conditions, followed by hesperidin extraction with a drastic pH change. Pectin had a high AUA content (66.20 \u00b1 1.25%), meeting the criteria for use in the food industry. The best-tested conditions for hesperidin extraction (30 min, 70 \u00b0C, 1:10 (w\/v)) provided a yield of 1% and a purity of 84%. The designed extraction scheme shows the potential of citrus waste as a source of bioactive compounds of good quality and high interest in the food industry while following the principles of green chemistry and circular economy.<\/jats:p>","DOI":"10.3390\/foods12203834","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T11:46:26Z","timestamp":1697715986000},"page":"3834","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["A Two-Step Approach to Orange Peel Waste Valorization: Consecutive Extraction of Pectin and Hesperidin"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5732-0994","authenticated-orcid":false,"given":"Onofre","family":"Figueira","sequence":"first","affiliation":[{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9200-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4216-8277","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Pereira","sequence":"additional","affiliation":[{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9200-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8303-4286","authenticated-orcid":false,"given":"Paula C.","family":"Castilho","sequence":"additional","affiliation":[{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9200-105 Funchal, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Russo, C., Maugeri, A., Lombardo, G.E., Musumeci, L., Barreca, D., Rapisarda, A., Cirmi, S., and Navarra, M. (2021). The Second Life of Citrus Fruit Waste: A Valuable Source of Bioactive Compounds. Molecules, 26.","DOI":"10.3390\/molecules26195991"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2488804","DOI":"10.1155\/2021\/2488804","article-title":"Citrus Genus and Its Waste Utilization: A Review on Health-Promoting Activities and Industrial Application","volume":"2021","author":"Khan","year":"2021","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"106677","DOI":"10.1016\/j.eiar.2021.106677","article-title":"Global Warming Potential of Food Waste through the Life Cycle Assessment: An Analytical Review","volume":"91","author":"Amicarelli","year":"2021","journal-title":"Environ. Impact Assess. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.26480\/gws.01.2021.17.20","article-title":"Food Wastage: Causes, Impacts and Solutions","volume":"5","author":"Pandey","year":"2021","journal-title":"Sci. Herit. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100215","DOI":"10.1016\/j.afres.2022.100215","article-title":"Citrus Pectins: Structural Properties, Extraction Methods, Modifications and Applications in Food Systems\u2014A Review","volume":"2","author":"Singhal","year":"2022","journal-title":"Appl. Food Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.resconrec.2017.10.032","article-title":"Citrus Processing Wastes: Environmental Impacts, Recent Advances, and Future Perspectives in Total Valorization","volume":"129","author":"Satari","year":"2018","journal-title":"Resour. Conserv. Recycl."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"8855","DOI":"10.1021\/acs.jafc.2c02655","article-title":"Valorization of Food Processing Waste to Produce Valuable Polyphenolics","volume":"70","author":"Chang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s12649-020-00982-x","article-title":"A High-Yield Process for Extraction of Hesperidin from Orange (Citrus sinensis L. Osbeck) Peels Waste, and Its Transformation to Diosmetin, A Valuable and Bioactive Flavonoid","volume":"12","author":"Victor","year":"2021","journal-title":"Waste Biomass Valorization"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.tifs.2023.05.012","article-title":"Trends in Valorization of Citrus By-Products from the Net-Zero Perspective: Green Processing Innovation Combined with Applications in Emission Reduction","volume":"137","author":"Xu","year":"2023","journal-title":"Trends Food Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"126154","DOI":"10.1016\/j.jhazmat.2021.126154","article-title":"Minimizing Hazardous Impact of Food Waste in a Circular Economy\u2014Advances in Resource Recovery through Green Strategies","volume":"416","author":"Usmani","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100050","DOI":"10.1016\/j.afres.2022.100050","article-title":"Current Applications of Citrus Fruit Processing Waste: A Scientific Outlook","volume":"2","author":"Suri","year":"2022","journal-title":"Appl. Food Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Nieto, G., Fern\u00e1ndez-L\u00f3pez, J., P\u00e9rez-\u00c1lvarez, J.A., Pe\u00f1alver, R., Ros-Berruezo, G., and Viuda-Martos, M. (2021). Valorization of Citrus Co-Products: Recovery of Bioactive Compounds and Application in Meat and Meat Products. Plants, 10.","DOI":"10.3390\/plants10061069"},{"key":"ref_13","first-page":"1","article-title":"Hesperidin and Hesperitin Preparation and Purification from Citrus sinensis Peels","volume":"7","author":"Lahmer","year":"2015","journal-title":"Pharma Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"611","DOI":"10.3851\/IMP3235","article-title":"Hesperidin Attenuates Influenza a Virus (H1N1) Induced Lung Injury in Rats through Its Anti-Inflammatory Effect","volume":"23","author":"Ding","year":"2018","journal-title":"Antivir. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.jsps.2018.12.008","article-title":"Plant-Derived Antiviral Drugs as Novel Hepatitis B Virus Inhibitors: Cell Culture and Molecular Docking Study","volume":"27","author":"Parvez","year":"2019","journal-title":"Saudi Pharm. J."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bellavite, P., and Donzelli, A. (2020). Hesperidin and SARS-CoV-2: New Light on the Healthy Function of Citrus Fruits. Antioxidants, 9.","DOI":"10.20944\/preprints202006.0321.v1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3855","DOI":"10.2147\/DDDT.S227499","article-title":"Hesperidin: A Therapeutic Agent for Obesity","volume":"2019","author":"Xiong","year":"2019","journal-title":"Drug Des. Devel. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Hajialyani, M., Hosein Farzaei, M., Echeverr\u00eda, J., Nabavi, S., Uriarte, E., and Sobarzo-S\u00e1nchez, E. (2019). Hesperidin as a Neuroprotective Agent: A Review of Animal and Clinical Evidence. Molecules, 24.","DOI":"10.3390\/molecules24030648"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"102741","DOI":"10.1155\/2013\/102741","article-title":"Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson\u2019s Disease","volume":"2013","author":"Tamilselvam","year":"2013","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"918665","DOI":"10.3389\/fphar.2022.918665","article-title":"Hesperidin Inhibits Lung Cancer In Vitro and In Vivo Through PinX1","volume":"13","author":"Yao","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"312","DOI":"10.4062\/biomolther.2015.139","article-title":"Hesperidin Attenuates Ultraviolet B-Induced Apoptosis by Mitigating Oxidative Stress in Human Keratinocytes","volume":"24","author":"Hewage","year":"2016","journal-title":"Biomol. Ther."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Stanisic, D., Liu, L.H.B., dos Santos, R.V., Costa, A.F., Dur\u00e1n, N., and Tasic, L. (2020). New Sustainable Process for Hesperidin Isolation and Anti-Ageing Effects of Hesperidin Nanocrystals. Molecules, 25.","DOI":"10.3390\/molecules25194534"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"104563","DOI":"10.1016\/j.arabjc.2023.104563","article-title":"Novel Grasshopper Protein\/Soy Protein Isolate\/Pullulan Ternary Blend with Hesperidin Derivative for Antimicrobial Edible Film","volume":"16","author":"Zhang","year":"2023","journal-title":"Arab. J. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4803","DOI":"10.1007\/s00253-023-12628-8","article-title":"Efficient Production of \u03b1-Monoglucosyl Hesperidin by Cyclodextrin Glucanotransferase from Bacillus Subtilis","volume":"107","author":"Zhou","year":"2023","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_25","unstructured":"MarketWatch (2023, February 26). Hesperidin Market 2023: Global Industry Share, Key Dynamics and Forecast to 2028. Available online: https:\/\/www.marketwatch.com\/press-release\/hesperidin-market-2023-global-industry-share-key-dynamics-and-forecast-to-2028-2023-02-15."},{"key":"ref_26","unstructured":"Mudgil, D. (2017). Dietary Fiber for the Prevention of Cardiovascular Disease, Academic Press."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Benassi, L., Alessandri, I., and Vassalini, I. (2021). Assessing Green Methods for Pectin Extraction from Waste Orange Peels. Molecules, 26.","DOI":"10.3390\/molecules26061766"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.aasci.2018.02.007","article-title":"Sequential Extraction of Bioactive Compounds from Tangerine (Citrus unshiu) Peel","volume":"16","author":"Tsitsagi","year":"2018","journal-title":"Ann. Agrar. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Freitas, C.M.P., Coimbra, J.S.R., Souza, V.G.L., and Sousa, R.C.S. (2021). Structure and Applications of Pectin in Food, Biomedical, and Pharmaceutical Industry: A Review. Coatings, 11.","DOI":"10.3390\/coatings11080922"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Roy, S., Priyadarshi, R., \u0141opusiewicz, \u0141., Biswas, D., Chandel, V., and Rhim, J.-W. (2023). Recent Progress in Pectin Extraction, Characterization, and Pectin-Based Films for Active Food Packaging Applications: A Review. Int. J. Biol. Macromol., 239.","DOI":"10.1016\/j.ijbiomac.2023.124248"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1002\/fsn3.2754","article-title":"Optimization and Characterization of Pectin Extracted from Banana and Papaya Mixed Peels Using Response Surface Methodology","volume":"10","author":"Mada","year":"2022","journal-title":"Food Sci. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kozio\u0142, A., \u015aroda-Pomianek, K., G\u00f3rniak, A., Wikiera, A., Cyprych, K., and Malik, M. (2022). Structural Determination of Pectins by Spectroscopy Methods. Coatings, 12.","DOI":"10.3390\/coatings12040546"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"107483","DOI":"10.1016\/j.foodhyd.2022.107483","article-title":"Pectin: New Science and Forthcoming Applications of the Most Valued Hydrocolloid","volume":"127","author":"Ciriminna","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_34","unstructured":"MarketWatch (2023, February 12). Pectin Market Scenario 2023 to 2028: New Developments, Economic Situation and Growth Projection. Available online: https:\/\/www.marketwatch.com\/press-release\/pectin-market-scenario-2023-to-2028-new-developments-economic-situation-and-growth-projection-2023-02-09."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.1007\/s11947-023-03059-4","article-title":"A Comparative Study of the Extraction of Pectin from Kinnow (Citrus reticulata) Peel Using Different Techniques","volume":"16","author":"Kumari","year":"2023","journal-title":"Food Bioprocess Technol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Padilla de la Rosa, J., Ruiz-Palomino, P., Arriola-Guevara, E., Garc\u00eda-Fajardo, J., Sandoval, G., and Guatemala-Morales, G. (2018). A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (Citrus aurantifolia Swingle) That Is Extendible to the Citrus Genus. Processes, 6.","DOI":"10.20944\/preprints201811.0413.v1"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.arabjc.2014.11.011","article-title":"A New Approach for Pectin Extraction: Electromagnetic Induction Heating","volume":"10","author":"Zouambia","year":"2017","journal-title":"Arab. J. Chem."},{"key":"ref_38","unstructured":"Labrada, A., Lobarbio, C.F., and Taboada, E. (July, January 30). Downstream Purification of Pectin Precipitated from Mango Peel Extract. Proceedings of the International Conference on Technological and Social Innovations 2018, London, UK."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3109\/13880209.2010.509734","article-title":"Antioxidant Capacity of Hesperidin from Citrus Peel Using Electron Spin Resonance and Cytotoxic Activity against Human Carcinoma Cell Lines","volume":"49","year":"2011","journal-title":"Pharm. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/s40030-021-00514-3","article-title":"Effect of Process Parameters on Extraction of Pectin from Sweet Lime Peels","volume":"102","author":"Siddiqui","year":"2021","journal-title":"J. Inst. Eng. India Ser. A"},{"key":"ref_41","unstructured":"Ranganna, S. (1995). HandBook of Analysis and Quality Control for Fruits and Vegetable Products, McGraw Hill Publishing Co.. [2nd ed.]."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1002\/fsn3.761","article-title":"Extraction and Process Optimization of Bael Fruit Pectin","volume":"6","author":"Maskey","year":"2018","journal-title":"Food Sci. Nutr."},{"key":"ref_43","first-page":"143","article-title":"Extraction and Characterisation of Pectin from Various Tropical Agrowastes","volume":"10","author":"Mohamed","year":"1995","journal-title":"ASEAN Food J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.foodhyd.2018.02.030","article-title":"Thermal Degradation of Citrus Pectin in Low-Moisture Environment\u2014Influence of Acidic and Alkaline Pre-Treatment","volume":"86","author":"Kastner","year":"2019","journal-title":"Food Hydrocoll."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s11095-008-9729-6","article-title":"Solubility, Stability, Physicochemical Characteristics and In Vitro Ocular Tissue Permeability of Hesperidin: A Natural Bioflavonoid","volume":"26","author":"Majumdar","year":"2009","journal-title":"Pharm. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s40003-019-00419-5","article-title":"Characterization of Pectin Extracted from Orange Peel Powder Using Microwave-Assisted and Acid Extraction Methods","volume":"9","author":"Kute","year":"2020","journal-title":"Agric. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/s42853-021-00084-z","article-title":"Extraction and Characterization of Pectin from Citrus sinensis Peel","volume":"46","author":"Kamal","year":"2021","journal-title":"J. Biosyst. Eng."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s10068-017-0302-0","article-title":"Characterization of Pectin Extracted from Banana Peels of Different Varieties","volume":"27","author":"Khamsucharit","year":"2018","journal-title":"Food Sci. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40543-018-0151-3","article-title":"Optimization of Oil and Pectin Extraction from Orange (Citrus sinensis) Peels: A Response Surface Approach","volume":"9","author":"Fakayode","year":"2018","journal-title":"J. Anal. Sci. Technol."},{"key":"ref_50","first-page":"17","article-title":"Extraction and Characterization of Pectin from Orange (Citrus sinensis), Lemon (Citrus limon) and Tangerine (Citrus tangerina)","volume":"1","author":"Twinomuhwezi","year":"2020","journal-title":"Am. J. Phys. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"19950","DOI":"10.1038\/s41598-020-76965-8","article-title":"Extraction of Flavanones from Immature Citrus unshiu Pomace: Process Optimization and Antioxidant Evaluation","volume":"10","author":"Kim","year":"2020","journal-title":"Sci. Rep."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/20\/3834\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:10:00Z","timestamp":1760130600000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/20\/3834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,19]]},"references-count":51,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["foods12203834"],"URL":"https:\/\/doi.org\/10.3390\/foods12203834","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,19]]}}}