{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T03:56:35Z","timestamp":1777089395938,"version":"3.51.4"},"reference-count":44,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq, Brazil)","doi-asserted-by":"publisher","award":["407764\/2013-5"],"award-info":[{"award-number":["407764\/2013-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq, Brazil)","doi-asserted-by":"publisher","award":["741962"],"award-info":[{"award-number":["741962"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq, Brazil)","doi-asserted-by":"publisher","award":["001\/2014"],"award-info":[{"award-number":["001\/2014"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Pessoal de N\u00edvel Superior (CAPES, Brazil)","doi-asserted-by":"publisher","award":["407764\/2013-5"],"award-info":[{"award-number":["407764\/2013-5"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Pessoal de N\u00edvel Superior (CAPES, Brazil)","doi-asserted-by":"publisher","award":["741962"],"award-info":[{"award-number":["741962"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Pessoal de N\u00edvel Superior (CAPES, Brazil)","doi-asserted-by":"publisher","award":["001\/2014"],"award-info":[{"award-number":["001\/2014"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o Amaz\u00f4nia de Amparo a Estudos e Pesquisas (FAPESPA, Brazil)","award":["407764\/2013-5"],"award-info":[{"award-number":["407764\/2013-5"]}]},{"name":"Funda\u00e7\u00e3o Amaz\u00f4nia de Amparo a Estudos e Pesquisas (FAPESPA, Brazil)","award":["741962"],"award-info":[{"award-number":["741962"]}]},{"name":"Funda\u00e7\u00e3o Amaz\u00f4nia de Amparo a Estudos e Pesquisas (FAPESPA, Brazil)","award":["001\/2014"],"award-info":[{"award-number":["001\/2014"]}]},{"name":"Federal University of Par\u00e1 (UFPA, Brazil)","award":["407764\/2013-5"],"award-info":[{"award-number":["407764\/2013-5"]}]},{"name":"Federal University of Par\u00e1 (UFPA, Brazil)","award":["741962"],"award-info":[{"award-number":["741962"]}]},{"name":"Federal University of Par\u00e1 (UFPA, Brazil)","award":["001\/2014"],"award-info":[{"award-number":["001\/2014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Tucupi is a broth derived from cassava roots which is produced after the spontaneous fermentation of manipueira (the liquid portion obtained by pressing cassava roots), followed by cooking. This product is widely consumed along with traditional dishes in the Brazilian Amazonia and is already used in different places worldwide. In this study, tucupi obtained from the markets of Bel\u00e9m (Par\u00e1, Brazil) and produced using agroindustrial (11 samples) and non-agroindustrial (11 samples) units were investigated to determine their physicochemical characteristics, total and free HCN contents, and free bioactive amine profiles. Most of the samples showed significant variations (p \u2264 0.05) in pH (2.82\u20134.67), total acidity (0.14\u20131.36 g lactic acid\/100 mL), reducing sugars (up to 2.33 g\/100 mL), and total sugars (up to 4.35 g\/100 mL). Regarding the amines, four biogenic amines (0.5\u20134.2 mg\/L tyramine, 1.0\u201323.1 mg\/L putrescine, 0.5\u201366.8 mg\/L histamine, and 0.6\u20132.9 mg\/L tryptamine) and one polyamine (0.4\u20131.7 mg\/L spermidine) were identified in the tucupi samples. Even in the tucupi produced using the agroindustrial units, which had quality seals provided by the local regulatory agency, high levels of biogenic amines (4.4\u201378.2 mg\/L) were observed, as well as high dosages of total (8.87\u2013114.66 mg\/L) and free (0.80\u201338.38 mg\/L) HCN. These facts highlight the need for better knowledge regarding the product manufacturing process to establish standardization and high-quality conditions for tucupi processing since high contents of biogenic amines and HCN are commonly associated with adverse health effects.<\/jats:p>","DOI":"10.3390\/foods12234333","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T03:58:02Z","timestamp":1701403082000},"page":"4333","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Total and Free Hydrogen Cyanide Content and Profile of Bioactive Amines in Commercial Tucupi, a Traditionally Derived Cassava Product Widely Consumed in Northern Brazil"],"prefix":"10.3390","volume":"12","author":[{"given":"Brenda de Nazar\u00e9 do Carmo","family":"Brito","sequence":"first","affiliation":[{"name":"Graduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, PA, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8434-5820","authenticated-orcid":false,"given":"Mayara Galv\u00e3o","family":"Martins","sequence":"additional","affiliation":[{"name":"Innovation, Development and Adaptation of Sustainable Technologies Research Group (GPIDATS), Mamirau\u00e1 Institute for Sustainable Development (IDSM), Tef\u00e9 69553-225, AM, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4549-3297","authenticated-orcid":false,"given":"Renan Campos","family":"Chist\u00e9","sequence":"additional","affiliation":[{"name":"Graduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, PA, Brazil"},{"name":"Faculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, PA, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8584-5859","authenticated-orcid":false,"given":"Alessandra Santos","family":"Lopes","sequence":"additional","affiliation":[{"name":"Graduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, PA, Brazil"},{"name":"Faculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, PA, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4137-0396","authenticated-orcid":false,"given":"Maria Beatriz Abreu","family":"Gloria","sequence":"additional","affiliation":[{"name":"Laboratory of Food Biochemistry-LBqA & LCQ, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3900-205X","authenticated-orcid":false,"given":"Rosinelson da Silva","family":"Pena","sequence":"additional","affiliation":[{"name":"Graduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, PA, Brazil"},{"name":"Faculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Par\u00e1 (UFPA), Bel\u00e9m 66075-110, PA, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s12042-011-9091-4","article-title":"Cassava production and pest management: Present and potential threats in a changing environment","volume":"5","author":"Bellotti","year":"2012","journal-title":"Trop. 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