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Both matrices were also pasteurized by high pressure processing (600 MPa, 3 min, 17 \u00b0C, HPP) to simulate commercial pasteurization followed by HS, in comparison with refrigeration (5 \u00b0C, RF). The results showed that, at AP\/RT, spores\u2019 development occurred, except at pH 4.50 in BHI-broth, while for RF, no changes occurred along storage. Under HS, at pH 4.50, neither spore development nor inactivation occurred, while at pH 6.00\/7.50, inactivation occurred (\u22482.0 and 1.0 logs at 200 MPa, respectively). Coconut water at AP\/RT faced an increase of 1.6 logs of C. perfringens spores after 15 days, while for RF, no spore development occurred, while the inactivation of spores under HS happened (\u22483 logs at 200 MPa). HPP prior to HS seems to promote a subsequent inactivation of C. perfringens spores in BHI-broth at pH 4.50, which is less evident for other pHs. For HPP coconut water, the inactivation levels under HS were lower (\u22482.0 logs at 200 MPa). The Weibull model well described the inactivation pattern observed. These results suggest that HS\/RT can be simultaneously used as a tool to avoid C. perfringens spores\u2019 development, as well as for its inactivation, without the application of high temperatures that are required to inactivate these spores.<\/jats:p>","DOI":"10.3390\/foods13121832","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T11:58:58Z","timestamp":1718107138000},"page":"1832","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of pH and High-Pressure Pasteurization on the Germination and Development of Clostridium perfringens Spores under Hyperbaric Storage versus Refrigeration"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5742-706X","authenticated-orcid":false,"given":"Carlos A.","family":"Pinto","sequence":"first","affiliation":[{"name":"Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology (LAQV-REQUIMTE), Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5951-9462","authenticated-orcid":false,"given":"Alireza","family":"Mousakhani Ganjeh","sequence":"additional","affiliation":[{"name":"Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology (LAQV-REQUIMTE), Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5630-3989","authenticated-orcid":false,"given":"Francisco J.","family":"Barba","sequence":"additional","affiliation":[{"name":"Research Group in Innovative Technologies for Sustainable Food (ALISOST), Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de Val\u00e8ncia, Avda. Vicent Andr\u00e9s Estell\u00e9s, s\/n, 46100 Burjassot, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5536-6056","authenticated-orcid":false,"given":"Jorge A.","family":"Saraiva","sequence":"additional","affiliation":[{"name":"Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology (LAQV-REQUIMTE), Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"McClane, B.A., Robertson, S.L., and Li, J. (2012). Clostridium Perfringens. 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