{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T02:25:32Z","timestamp":1769826332509,"version":"3.49.0"},"reference-count":49,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T00:00:00Z","timestamp":1724198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Natural pigments extracted from plant species are used in foods, cosmetics, and pharmaceuticals. This study evaluates the comprehensive biological activities of anthocyanins isolated from Andean blueberry (Vaccinium floribundum Kunth) and Andean blackberry (Rubus glaucus Benth), focusing on their antimicrobial, antioxidant, antitumoral, anti-inflammatory, and hemolytic properties. Chemical characterization revealed significant anthocyanin content with complex mass spectrometric profiles indicating diverse glycosylation patterns that may influence their bioactivity. The antimicrobial assays showed that the extracts were particularly effective against Gram-positive bacteria, with minimal inhibitory concentrations (MICs) as low as 1 mg\/mL for Rubus glaucus, indicating strong potential for therapeutic use. The antioxidant capacity of the berries was substantial, albeit slightly lower than that of ascorbic acid. The extracts also exhibited notable antitumoral activity in various cancer cell lines, showing promise as adjunctive or preventive treatments. The anti-inflammatory effects were confirmed by inhibiting nitric oxide production in macrophage cells, highlighting their potential in managing inflammatory diseases. In terms of hemolytic activity, Rubus glaucus exhibited dose-dependent effects, potentially attributable to anthocyanins and phenolics, while Vaccinium floribundum demonstrated no significant hemolytic activity, underscoring its safety. These findings suggest that anthocyanins from Andean berries possess potent biological activities, which could be leveraged for health benefits in pharmaceutical and nutraceutical applications. Further studies are needed to isolate specific bioactive compounds and investigate their synergistic effects in clinical and real-world contexts.<\/jats:p>","DOI":"10.3390\/foods13162625","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T04:26:57Z","timestamp":1724300817000},"page":"2625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Exploring the Multifaceted Biological Activities of Anthocyanins Isolated from Two Andean Berries"],"prefix":"10.3390","volume":"13","author":[{"given":"Carlos","family":"Barba-Ostria","sequence":"first","affiliation":[{"name":"Escuela de Medicina, Colegio de Ciencias de la Salud, Universidad San Francisco de Quito USFQ, Quito 170901, Ecuador"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2351-5595","authenticated-orcid":false,"given":"Saskya E.","family":"Carrera-Pacheco","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170147, Ecuador"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5630-6645","authenticated-orcid":false,"given":"Rebeca","family":"Gonzalez-Pastor","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170147, Ecuador"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9037-9306","authenticated-orcid":false,"given":"Johana","family":"Zu\u00f1iga-Miranda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170147, Ecuador"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0220-4990","authenticated-orcid":false,"given":"Arianna","family":"Mayorga-Ramos","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170147, Ecuador"}]},{"given":"Eduardo","family":"Tejera","sequence":"additional","affiliation":[{"name":"Bio-Cheminformatics Research Group, Universidad de Las Am\u00e9ricas, Quito 170504, Ecuador"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3756-2387","authenticated-orcid":false,"given":"Linda P.","family":"Guam\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170147, Ecuador"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3390\/cimb43010004","article-title":"Genetic Differentiation in Anthocyanin Content among Berry Fruits","volume":"43","author":"Ponder","year":"2021","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3233\/BR-2010-008","article-title":"Maximising blackcurrant anthocyanins: Temporal changes during ripening and storage in different genotypes","volume":"1","author":"Chope","year":"2010","journal-title":"J. Berry Res."},{"key":"ref_3","first-page":"9453","article-title":"Extraction of anthocyanins from Morti\u00f1o (Vaccinium floribundum) and determination of their antioxidant capacity","volume":"74","author":"Endara","year":"2021","journal-title":"Rev. Fac. Nac. Agron. Medell\u00edn"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8966","DOI":"10.1021\/jf100975m","article-title":"Antioxidant capacity and in vitro inhibition of adipogenesis and inflammation by phenolic extracts of Vaccinium floribundum and Aristotelia chilensis","volume":"58","author":"Schreckinger","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"547","DOI":"10.32628\/IJSRST2293107","article-title":"A review on anthocyanins: Coloured pigments as food, pharmaceutical ingredients and the potential health benefits","volume":"9","author":"Chikane","year":"2022","journal-title":"Int. J. Sci. Res. Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Merecz-Sadowska, A., Sitarek, P., Kowalczyk, T., Zajdel, K., J\u0119cek, M., Nowak, P., and Zajdel, R. (2023). Food Anthocyanins: Malvidin and Its Glycosides as Promising Antioxidant and Anti-Inflammatory Agents with Potential Health Benefits. Nutrients, 15.","DOI":"10.3390\/nu15133016"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Marques-da-Silva, D., Rodrigues, J.R., and Lagoa, R. (2021). Anthocyanins, effects in mitochondria and metabolism. Mitochondrial Physiology and Vegetal Molecules, Elsevier.","DOI":"10.1016\/B978-0-12-821562-3.00028-9"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/fst.39119","article-title":"Obesity-associated Pathways of Anthocyanins","volume":"41","author":"Yildiz","year":"2021","journal-title":"Food Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e16474","DOI":"10.1111\/jfpp.16474","article-title":"Preservation of color and nutrients in anthocyanin-rich edible flowers: Progress of new extraction and processing techniques","volume":"46","author":"Barani","year":"2022","journal-title":"J. Food Process. Preserv."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7573","DOI":"10.1111\/ijfs.16132","article-title":"Anthocyanin: A review of plant sources, extraction, stability, content determination and modifications","volume":"57","author":"Guo","year":"2022","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1093\/jaoac\/88.5.1269","article-title":"Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study","volume":"88","author":"Lee","year":"2005","journal-title":"J. AOAC Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"113039","DOI":"10.1016\/j.fct.2022.113039","article-title":"Metabolomic profile and computational analysis for the identification of the potential anti-inflammatory mechanisms of action of the traditional medicinal plants Ocimum basilicum and Ocimum tenuiflorum","volume":"164","author":"Guevara","year":"2022","journal-title":"Food Chem. Toxicol."},{"key":"ref_13","unstructured":"CLSI (2022, May 26). Dilution AST for Aerobically Grown Bacteria\u2014CLSI. Available online: https:\/\/clsi.org\/standards\/products\/microbiology\/documents\/m07\/."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Cadena-Cruz, J.E., Guam\u00e1n-Ortiz, L.M., Romero-Benavides, J.C., Bailon-Moscoso, N., Murillo-Sotomayor, K.E., Ortiz-Guam\u00e1n, N.V., and Heredia-Moya, J. (2021). Synthesis of 4,4\u2032-(arylmethylene)bis(3-methyl-1-phenyl-1H-pyrazol-5-ols) and evaluation of their antioxidant and anticancer activities. BMC Chem., 15.","DOI":"10.1186\/s13065-021-00765-y"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"S\u00e6b\u00f8, I.P., Bj\u00f8r\u00e5s, M., Franzyk, H., Helgesen, E., and Booth, J.A. (2023). Optimization of the hemolysis assay for the assessment of cytotoxicity. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032914"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Spinardi, A., Cola, G., Gardana, C.S., and Mignani, I. (2019). Variation of anthocyanin content and profile throughout fruit development and ripening of highbush blueberry cultivars grown at two different altitudes. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.01045"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Guevara-Ter\u00e1n, M., Padilla-Arias, K., Beltr\u00e1n-Novoa, A., Gonz\u00e1lez-Param\u00e1s, A.M., Giampieri, F., Battino, M., V\u00e1squez-Castillo, W., Fernandez-Soto, P., Tejera, E., and Alvarez-Suarez, J.M. (2022). Influence of Altitudes and Development Stages on the Chemical Composition, Antioxidant, and Antimicrobial Capacity of the Wild Andean Blueberry (Vaccinium floribundum Kunth). Molecules, 27.","DOI":"10.3390\/molecules27217525"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Wu, H., Di, Q.-R., Zhong, L., Zhou, J.-Z., Shan, C.-J., Liu, X.-L., and Ma, A.-M. (2022). Enhancement on antioxidant, anti-hyperglycemic and antibacterial activities of blackberry anthocyanins by processes optimization involving extraction and purification. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1007691"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Liu, H., Wu, H., Wang, Y., Wang, F., Liu, X., and Zhou, J. (2021). Enhancement on antioxidant and antibacterial activities of Brightwell blueberry by extraction and purification. Appl. Biol. Chem., 64.","DOI":"10.1186\/s13765-021-00649-8"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Aita, S., Capriotti, A., Cavaliere, C., Cerrato, A., Giannelli Moneta, B., Montone, C., Piovesana, S., and Lagan\u00e0, A. (2021). Andean Blueberry of the Genus Disterigma: A High-Resolution Mass Spectrometric Approach for the Comprehensive Characterization of Phenolic Compounds. Separations, 8.","DOI":"10.3390\/separations8050058"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Stein-Chisholm, R., Beaulieu, J., Grimm, C., and Lloyd, S. (2017). LC\u2013MS\/MS and UPLC\u2013UV Evaluation of Anthocyanins and Anthocyanidins during Rabbiteye Blueberry Juice Processing. Beverages, 3.","DOI":"10.3390\/beverages3040056"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.phytochem.2016.04.004","article-title":"Anthocyanins of the anthers as chemotaxonomic markers in the genus Populus L. Differentiation between Populus nigra, Populus alba and Populus tremula","volume":"128","year":"2016","journal-title":"Phytochemistry"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1002\/fsn3.638","article-title":"Chemical, antimicrobial, and molecular characterization of morti\u00f1o (Vaccinium floribundum Kunth) fruits and leaves","volume":"6","author":"Llivisaca","year":"2018","journal-title":"Food Sci. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e03845","DOI":"10.1016\/j.heliyon.2020.e03845","article-title":"Phenolic profile, in vitro antimicrobial activity and antioxidant capacity of Vaccinium meridionale swartz pomace","volume":"6","author":"Soto","year":"2020","journal-title":"Heliyon"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/jac\/48.suppl_1.5","article-title":"Determination of minimum inhibitory concentrations","volume":"48","author":"Andrews","year":"2001","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Urbonaviciene, D., Bobinaite, R., Viskelis, P., Bobinas, C., Petruskevicius, A., Klavins, L., and Viskelis, J. (2022). Geographic Variability of Biologically Active Compounds, Antioxidant Activity and Physico-Chemical Properties in Wild Bilberries (Vaccinium myrtillus L.). Antioxidants, 11.","DOI":"10.3390\/antiox11030588"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"68","DOI":"10.22159\/ijpps.2018v10i6.18649","article-title":"Microbicidal potentiality of purified anthocyanin from in vitro culture of clerodendron infortunatum L. against selected pathogens","volume":"10","author":"Vg","year":"2018","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sharma, N., Tiwari, V., Vats, S., Kumari, A., Chunduri, V., Kaur, S., Kapoor, P., and Garg, M. (2020). Evaluation of Anthocyanin Content, Antioxidant Potential and Antimicrobial Activity of Black, Purple and Blue Colored Wheat Flour and Wheat-Grass Juice against Common Human Pathogens. Molecules, 25.","DOI":"10.3390\/molecules25245785"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1002\/ptr.4592","article-title":"Effects of cranberry extracts on growth and biofilm production of Escherichia coli and Staphylococcus species","volume":"26","author":"LaPlante","year":"2012","journal-title":"Phytother. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1111\/jam.13215","article-title":"Antimicrobial, antiadhesive and antibiofilm activity of an ethanolic, anthocyanin-rich blueberry extract purified by solid phase extraction","volume":"121","author":"Silva","year":"2016","journal-title":"J. Appl. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"655","DOI":"10.15255\/KUI.2017.041","article-title":"Extraction, separation, and purification of blueberry anthocyanin using ethyl alcohol","volume":"66","author":"Gao","year":"2017","journal-title":"Kem. Ind."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Cairone, F., Simonetti, G., Orekhova, A., Casadei, M.A., Zengin, G., and Cesa, S. (2021). Health Potential of Clery Strawberries: Enzymatic Inhibition and Anti-Candida Activity Evaluation. Molecules, 26.","DOI":"10.3390\/molecules26061731"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Salaheen, S., Peng, M., Joo, J., Teramoto, H., and Biswas, D. (2017). Eradication and Sensitization of Methicillin Resistant Staphylococcus aureus to Methicillin with Bioactive Extracts of Berry Pomace. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00253"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Quave, C.L., Est\u00e9vez-Carmona, M., Compadre, C.M., Hobby, G., Hendrickson, H., Beenken, K.E., and Smeltzer, M.S. (2012). Ellagic acid derivatives from Rubus ulmifolius inhibit Staphylococcus aureus biofilm formation and improve response to antibiotics. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0028737"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3233\/JBR-180316","article-title":"Wild Andean blackberry (Rubus glaucus Benth) and Andean blueberry (Vaccinium floribundum Kunth) from the Highlands of Ecuador: Nutritional composition and protective effect on human dermal fibroblasts against cytotoxic oxidative damage","volume":"8","author":"Iturralde","year":"2018","journal-title":"J. Berry Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.jpba.2013.11.016","article-title":"Metabolite profiling of polyphenols in Vaccinium berries and determination of their chemopreventive properties","volume":"89","author":"Prencipe","year":"2014","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Samaniego, I., Brito, B., Viera, W., Cabrera, A., Llerena, W., Kannangara, T., Vilcacundo, R., Ang\u00f3s, I., and Carrillo, W. (2020). Influence of the Maturity Stage on the Phytochemical Composition and the Antioxidant Activity of Four Andean Blackberry Cultivars (Rubus glaucus Benth) from Ecuador. Plants, 9.","DOI":"10.3390\/plants9081027"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"8274","DOI":"10.1021\/jf9013586","article-title":"Chemical composition and phenolic compound profile of morti\u00f1o (Vaccinium floribundum Kunth)","volume":"57","author":"Vasco","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Gil-Mart\u00ednez, L., Mut-Salud, N., Ruiz-Garc\u00eda, J.A., Falc\u00f3n-Pi\u00f1eiro, A., Maij\u00f3-Ferr\u00e9, M., Ba\u00f1os, A., De la Torre-Ram\u00edrez, J.M., Guillam\u00f3n, E., Verardo, V., and G\u00f3mez-Caravaca, A.M. (2023). Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits. Foods, 12.","DOI":"10.3390\/foods12071505"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"101","DOI":"10.32471\/exp-oncology.2312-8852.vol-42-no-2.14766","article-title":"Anti-proliferative effects of a blueberry extract on a panel of tumor cell lines of different origin","volume":"42","author":"Lamdan","year":"2020","journal-title":"Exp. Oncol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1080\/01635581.2021.1952454","article-title":"Epigenetic effects of blackberry extract on human colorectal cancer cells","volume":"74","author":"Tatar","year":"2022","journal-title":"Nutr. Cancer"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/ijfs.13923","article-title":"Polyphenolic composition, antioxidant and antiproliferative effects of wild and cultivated blackberries (Rubus fruticosus L.) pomace","volume":"54","year":"2019","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1111\/bph.13627","article-title":"Effects of anthocyanins on the prevention and treatment of cancer","volume":"174","author":"Lin","year":"2017","journal-title":"Br. J. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.lwt.2014.10.010","article-title":"Protective effect of blueberry anthocyanins in a CCL4-induced liver cell model","volume":"60","author":"Chen","year":"2015","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Gu, I., Brownmiller, C., Stebbins, N.B., Mauromoustakos, A., Howard, L., and Lee, S.-O. (2020). Berry Phenolic and Volatile Extracts Inhibit Pro-Inflammatory Cytokine Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-\u03baB Signaling Pathway. Antioxidants, 9.","DOI":"10.3390\/antiox9090871"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1862462","DOI":"10.1155\/2018\/1862462","article-title":"Antioxidant and Anti-Inflammatory Effects of Blueberry Anthocyanins on High Glucose-Induced Human Retinal Capillary Endothelial Cells","volume":"2018","author":"Huang","year":"2018","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Martins, M.S., Gon\u00e7alves, A.C., Alves, G., and Silva, L.R. (2023). Blackberries and Mulberries: Berries with Significant Health-Promoting Properties. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241512024"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1021\/jf072504n","article-title":"Berry fruits for cancer prevention: Current status and future prospects","volume":"56","author":"Seeram","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"7449","DOI":"10.1021\/jf0207530","article-title":"Antioxidant and antiproliferative activities of common fruits","volume":"50","author":"Sun","year":"2002","journal-title":"J. Agric. Food Chem."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/16\/2625\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:40:37Z","timestamp":1760110837000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/16\/2625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,21]]},"references-count":49,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["foods13162625"],"URL":"https:\/\/doi.org\/10.3390\/foods13162625","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,21]]}}}