{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T07:10:28Z","timestamp":1777014628593,"version":"3.51.4"},"reference-count":71,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Funds from FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UID\/Multi\/50016\/2020"],"award-info":[{"award-number":["UID\/Multi\/50016\/2020"]}]},{"name":"National Funds from FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["MAR-04.03.01-FEAMP-0498"],"award-info":[{"award-number":["MAR-04.03.01-FEAMP-0498"]}]},{"name":"National Funds from FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UI\/BD\/152825\/2022"],"award-info":[{"award-number":["UI\/BD\/152825\/2022"]}]},{"name":"Programa Operacional do Mar and FEAMP\u2014Fundo Europeu Assuntos Mar\u00edtimos e das Pescas","award":["UID\/Multi\/50016\/2020"],"award-info":[{"award-number":["UID\/Multi\/50016\/2020"]}]},{"name":"Programa Operacional do Mar and FEAMP\u2014Fundo Europeu Assuntos Mar\u00edtimos e das Pescas","award":["MAR-04.03.01-FEAMP-0498"],"award-info":[{"award-number":["MAR-04.03.01-FEAMP-0498"]}]},{"name":"Programa Operacional do Mar and FEAMP\u2014Fundo Europeu Assuntos Mar\u00edtimos e das Pescas","award":["UI\/BD\/152825\/2022"],"award-info":[{"award-number":["UI\/BD\/152825\/2022"]}]},{"name":"Portuguese government","award":["UID\/Multi\/50016\/2020"],"award-info":[{"award-number":["UID\/Multi\/50016\/2020"]}]},{"name":"Portuguese government","award":["MAR-04.03.01-FEAMP-0498"],"award-info":[{"award-number":["MAR-04.03.01-FEAMP-0498"]}]},{"name":"Portuguese government","award":["UI\/BD\/152825\/2022"],"award-info":[{"award-number":["UI\/BD\/152825\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Fucus spp. seaweeds thrive in the cold temperate waters of the northern hemisphere, specifically in the littoral and sublittoral regions along rocky shorelines. Moreover, they are known to be a rich source of bioactive compounds. This study explored the valorization of Fucus spiralis through the extraction of bioactives and polysaccharides (PSs) for food applications and biostimulant use. The bioactives were extracted using microwave hydrodiffusion and gravity (MHG), where the condition of 300 W for 20 min resulted in the highest total phenolic content and antioxidant activity of the extract. Cellular assays confirmed that the extract, at 0.5 mg\/mL, was non-cytotoxic to HaCat cells. Polysaccharides (PSs) were extracted from the remaining biomass. The residue from this second extraction contained 1.5% protein and 13.35% carbohydrates. Additionally, the free amino acids and minerals profiles of both solid residues were determined. An edible film was formulated using alginate (2%), PS-rich Fucus spiralis extract (0.5%), and F. spiralis bioactive-rich extract (0.25%). The film demonstrated significant antioxidant properties, with ABTS and DPPH values of 221.460 \u00b1 10.389 and 186.889 \u00b1 36.062 \u00b5M TE\/mg film, respectively. It also exhibited notable physical characteristics, including high water vapor permeability (11.15 \u00b1 1.55 g.mm.m\u22122.day\u22121.kPa\u22121) and 100% water solubility. The residues from both extractions of Fucus spiralis exhibited biostimulant (BS) effects on seed germination and seedling growth. BSs with PSs enhanced pea germination by 48%, while BSs without PSs increased the root dry weight of rice and tomato by 53% and up to 176%, respectively, as well as the shoot dry weight by up to 38% and up to 74%, respectively. These findings underscore the potential of Fucus spiralis within the framework of a circular economy, wherein both extracted bioactives and post-extraction by-products can be used for sustainable agriculture and food applications.<\/jats:p>","DOI":"10.3390\/foods13182938","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T00:51:43Z","timestamp":1726620703000},"page":"2938","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Integrated Valorization of Fucus spiralis Alga: Polysaccharides and Bioactives for Edible Films and Residues as Biostimulants"],"prefix":"10.3390","volume":"13","author":[{"given":"Valter F. R.","family":"Martins","sequence":"first","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6542-175X","authenticated-orcid":false,"given":"Marta","family":"Coelho","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7705-2293","authenticated-orcid":false,"given":"Manuela","family":"Machado","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3121-4514","authenticated-orcid":false,"given":"Eduardo","family":"Costa","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7883-2446","authenticated-orcid":false,"given":"Ana M.","family":"Gomes","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6598-6515","authenticated-orcid":false,"given":"F\u00e1tima","family":"Po\u00e7as","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"given":"Raul A.","family":"Sperotto","sequence":"additional","affiliation":[{"name":"Graduate Program in Plant Physiology, Botany Department, Biology Institute, Federal University of Pelotas, Pelotas 96160-000, Brazil"}]},{"given":"Elena","family":"Rosa-Martinez","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"given":"Marta","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0760-3184","authenticated-orcid":false,"given":"Manuela E.","family":"Pintado","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5138-6941","authenticated-orcid":false,"given":"Rui M. S. C.","family":"Morais","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1059-9649","authenticated-orcid":false,"given":"Alcina M. M. B.","family":"Morais","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Pereira, L. (2009). 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