{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T22:37:32Z","timestamp":1776465452484,"version":"3.51.2"},"reference-count":66,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"national funds (FCT\/ MCTES) through the project PTDC\/BAA-AGR\/4923\/2021 \u2018NanoProMilk\u2019"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Background\/Objectives: Peptides from protein ingredients exhibit key biological activities, including antimicrobial, antihypertensive, antioxidant, anti-inflammatory, analgesic, and immunomodulatory effects. Aligning with the One Health approach, there is growing investment in promoting pet health and well-being. As a result, sustainable functional ingredients are increasingly essential for pet food development. In this work, peptides derived from lactoferrins of different mammalian species were synthesized and their antioxidant, anti-inflammatory, and antihypertensive activities were investigated. Methods: This study examined the antioxidant, anti-inflammatory, antihypertensive activities, and cytotoxicity of bioactive peptides derived from lactoferrins of various mammalian species through spectroscopical methods. The peptides were produced via chemical synthesis (bottom-up approach). Results: Peptides derived from bovine lactoferrin showed the most promising antioxidant and anti-inflammatory activities, whereas those derived from human lactoferrin showed the highest antihypertensive effects and the lowest cytotoxicity. In short, milk-derived peptides with antioxidant, anti-inflammatory, and antihypertensive activity were identified. Conclusions: This motivates further studies to better characterize these peptides, including their properties and pharmacokinetics in vivo, to assess their true potential as nutraceutical agents.<\/jats:p>","DOI":"10.3390\/foods14030535","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T08:57:46Z","timestamp":1738832266000},"page":"535","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bioactive Peptides from Milk Proteins with Antioxidant, Anti-Inflammatory, and Antihypertensive Activities"],"prefix":"10.3390","volume":"14","author":[{"given":"Tha\u00eds","family":"Borges","sequence":"first","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, S\/N, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2343-1108","authenticated-orcid":false,"given":"Pedro","family":"Coelho","sequence":"additional","affiliation":[{"name":"RISE-Health, Center for Translational Health and Medical Biotechnology Research (TBIO), ESS, Polytechnic of Porto, R. Dr. Ant\u00f3nio Bernardino de Almeida, 400, 4200-072 Porto, Portugal"},{"name":"Chemical and Biomolecular Sciences, School of Health (ESS), Polytechnic Institute of Porto, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9920-936X","authenticated-orcid":false,"given":"Cristina","family":"Prud\u00eancio","sequence":"additional","affiliation":[{"name":"RISE-Health, Center for Translational Health and Medical Biotechnology Research (TBIO), ESS, Polytechnic of Porto, R. Dr. Ant\u00f3nio Bernardino de Almeida, 400, 4200-072 Porto, Portugal"},{"name":"Chemical and Biomolecular Sciences, School of Health (ESS), Polytechnic Institute of Porto, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5499-7112","authenticated-orcid":false,"given":"Ana","family":"Gomes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, S\/N, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6018-4724","authenticated-orcid":false,"given":"Paula","family":"Gomes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, S\/N, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1761-117X","authenticated-orcid":false,"given":"Ricardo","family":"Ferraz","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, S\/N, 4169-007 Porto, Portugal"},{"name":"RISE-Health, Center for Translational Health and Medical Biotechnology Research (TBIO), ESS, Polytechnic of Porto, R. Dr. Ant\u00f3nio Bernardino de Almeida, 400, 4200-072 Porto, Portugal"},{"name":"Chemical and Biomolecular Sciences, School of Health (ESS), Polytechnic Institute of Porto, 4200-072 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3585","DOI":"10.1021\/acs.jafc.1c06289","article-title":"Bioactive Peptides: From Basic Research to Clinical Trials and Commercialization","volume":"70","author":"Duffuler","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hajam, Y., Rani, R., Ganie, S., Sheikh, T., Javaid, D., Qadri, S., Pramodh, S., Alsulimani, A., Alkhanani, M., and Harakeh, S. (2022). Oxidative Stress in Human Pathology and Aging: Molecular Mechanisms and Perspectives. 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