{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T03:37:57Z","timestamp":1773113877980,"version":"3.50.1"},"reference-count":119,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT","award":["UID\/04033"],"award-info":[{"award-number":["UID\/04033"]}]},{"name":"FCT","award":["LA\/P\/0126\/2020"],"award-info":[{"award-number":["LA\/P\/0126\/2020"]}]},{"name":"FCT","award":["UIDB\/00772\/2020"],"award-info":[{"award-number":["UIDB\/00772\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>This review presents an in-depth analysis of the latest methods used for the determination of sucralose (E955), focusing on research conducted over the past 10 years. As a widely used sugar substitute in the food and pharmaceutical industries, sucralose has raised concerns about its environmental persistence, potential genotoxicity, and health impacts. This study examines several spectrophotometric, chromatographic, and electrochemical techniques, evaluating their sensitivity, selectivity, and limitations in differentiating sucralose from natural carbohydrates and other sweeteners. The review highlights the pressing need for novel detection methods that not only improve accuracy in trace detection but also address growing concerns about its bioaccumulation and conversion into harmful metabolites. Advancing these analytical techniques is essential for enhancing food safety, public health surveillance, and environmental risk assessment. Chromatographic methods are dominant in sucralose determination in foods and environmental objects, as they allow the determination of sucralose at micro- and nanomolar levels. However, spectrophotometric and electrochemical methods are frequently used as complementary to chromatographic methodologies, sensitizing them. On the other hand, purely spectrophotometric methods are less popular, and electrochemical methods remain underdeveloped. Therefore, the advancement of sucralose determination must be due to cheaper chromatographic and classical electrochemical methods.<\/jats:p>","DOI":"10.3390\/foods14071267","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T03:36:45Z","timestamp":1743737805000},"page":"1267","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Advancements and Challenges in Sucralose Determination: A Comparative Review of Chromatographic, Electrochemical, and Spectrophotometric Methods"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7696-0954","authenticated-orcid":false,"given":"Volodymyr V.","family":"Tkach","sequence":"first","affiliation":[{"name":"General and Material Chemistry Department, Chernivtsi National University, Kotrsyubynsky Str. 2, 58000 Chernivtsi, Ukraine"},{"name":"Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s\/n, 4200-065 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4836-1035","authenticated-orcid":false,"given":"Tetiana V.","family":"Morozova","sequence":"additional","affiliation":[{"name":"Ecology and Environmental Protection Department, National Transport University, Omelianovych-Pavlenko Str. 1, 01001 Kyiv, Ukraine"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4461-7021","authenticated-orcid":false,"given":"Isabel O\u2019Neill","family":"de Mascarenhas Gaiv\u00e3o","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Research Centre (CECAV), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Yana G.","family":"Ivanushko","sequence":"additional","affiliation":[{"name":"Disaster and Military Medicine Department, Bukovinian State Medical University, Teatralna Sq. 9, 58001 Chernivtsi, Ukraine"}]},{"given":"Jos\u00e9 In\u00e1cio Ferr\u00e3o","family":"da Paiva Martins","sequence":"additional","affiliation":[{"name":"Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s\/n, 4200-065 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5834-6141","authenticated-orcid":false,"given":"Ana Novo","family":"Barros","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University de Tra\u0301s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e071204","DOI":"10.1136\/bmj-2022-071204","article-title":"Artificial sweeteners and risk of cardiovascular diseases: Results from the prospective NutriNet-Sant\u00e9 cohort","volume":"378","author":"Debras","year":"2022","journal-title":"BMJ"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ju\u00e1rez, G., Sanz-Novo, M., Alonso, J.L., Alonso, E.R., and Le\u00f3n, I. 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