{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:23:12Z","timestamp":1760059392936,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T00:00:00Z","timestamp":1749427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT) and the European Social Fund (FSE)","award":["https:\/\/doi.org\/10.54499\/2020.06239.BD","NORTE-01-0145-FEDER-000066","UIDB\/50016\/2020"],"award-info":[{"award-number":["https:\/\/doi.org\/10.54499\/2020.06239.BD","NORTE-01-0145-FEDER-000066","UIDB\/50016\/2020"]}]},{"name":"European Regional Development Fund (ERDF) through the Northern Regional Operational Program","award":["https:\/\/doi.org\/10.54499\/2020.06239.BD","NORTE-01-0145-FEDER-000066","UIDB\/50016\/2020"],"award-info":[{"award-number":["https:\/\/doi.org\/10.54499\/2020.06239.BD","NORTE-01-0145-FEDER-000066","UIDB\/50016\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["https:\/\/doi.org\/10.54499\/2020.06239.BD","NORTE-01-0145-FEDER-000066","UIDB\/50016\/2020"],"award-info":[{"award-number":["https:\/\/doi.org\/10.54499\/2020.06239.BD","NORTE-01-0145-FEDER-000066","UIDB\/50016\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Vegetables are commodities frequently sold in local markets and have been associated with foodborne outbreaks in short and local supply outlets worldwide. These outbreaks could potentially be mitigated through the implementation of good handling practices (GHPs) at points of sale. Numerous studies have assessed microbiological contamination in small-scale vegetable outlets in developing countries. In contrast, research on these risks in developed countries is comparatively scarce. However, with the increasing demand for vegetables, along with the increasing popularity of local markets, there is potential for an increase in foodborne outbreaks in developed countries. This study aimed to perform a microbiological assessment in local and short supply chain outlets of farmers in Portugal, as a case study, and to observe behaviors regarding GHPs in these outlets. The study was performed before and after the implementation of improved GHPs. This research employed quantitative analysis to measure the microbial load on vegetables, bench surfaces, and vendors\u2019 hands. Additionally, a qualitative analysis was conducted to understand farmers\u2019 behavior regarding GHPs using observational methods. Microbial hazards were detected in vegetables, on surfaces, and on hands both before and after the implementation of these practices, although the implementation of GHPs reduced the number of contaminations potentially associated with the practices used at the outlets. The results of this study highlight the importance of implementing GHPs in local and short supply chain markets for vegetables and fruits in developed countries, not only to protect consumers\u2019 health, but also the farmers\u2019 businesses.<\/jats:p>","DOI":"10.3390\/foods14122036","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T08:22:34Z","timestamp":1749457354000},"page":"2036","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Case Study on the Microbiological Consequences of Short Supply Chains in High-Income Countries\u2014The Consequences of Good Handling Practices (GHPs) in Vegetable Outlets in Portugal"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5267-5198","authenticated-orcid":false,"given":"Ariana","family":"Macieira","sequence":"first","affiliation":[{"name":"CBQF-Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8857-6471","authenticated-orcid":false,"given":"Teresa R. S.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"CBQF-Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6296-5137","authenticated-orcid":false,"given":"Paula","family":"Teixeira","sequence":"additional","affiliation":[{"name":"CBQF-Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jrurstud.2007.12.009","article-title":"Unpacking the terms of engagement with local food at the Farmers\u2019 Market: Insights from Ontario","volume":"24","author":"Smithers","year":"2008","journal-title":"J. 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