{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:18:41Z","timestamp":1766708321995,"version":"3.48.0"},"reference-count":48,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s NextGenerationEU\/PRTR","award":["MCIN\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033"]}]},{"name":"European Union\u2019s NextGenerationEU\/PRTR","award":["FJC2021-046684-I"],"award-info":[{"award-number":["FJC2021-046684-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The rising global prevalence of obesity and metabolic disorders calls for innovative dietary strategies that can modulate key enzymatic pathways involved in lipid and carbohydrate metabolism. This study uncovers the effects of sulforaphane (SFN)-rich broccoli-derived formulations\u2014including liquid and lyophilised forms, as well as two commercial prototypes, Sulforaphan\u00ae BASIC and Sulforaphan\u00ae SMART, the latter being characterised by the inclusion of an enteric-coated myrosinase enzyme designed to enhance the in situ conversion of glucosinolates (GSL) into bioactive isothiocyanates (ITC)\u2014on lipid and carbohydrate metabolism in 3T3-L1 adipocytes. Across the formulations, total GSL content ranged widely, with GS0 showing the highest levels. Functionally, all SFN-rich formulations significantly reduced intracellular triglyceride content, with the SMART formulation achieving the strongest reduction (11% compared with untreated controls). Across enzymatic assays, we recorded that every formulation inhibited lipoprotein lipase and \u03b1-glucosidase activities, with Sulforaphan\u00ae BASIC and Sulforaphan\u00ae SMART leading to moderate inhibition (40\u201350%). The potent effect of SMART formulation may be associated with the presence of enteric-coated myrosinase, which enhances the conversion of GSL into bioactive ITC. The gathered evidence provides further insights into the potential of bioactive compounds in cruciferous foods to modulate metabolic health, underscoring their potential role in complementary therapeutic strategies for obesity and its comorbidities.<\/jats:p>","DOI":"10.3390\/foods15010013","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T07:01:33Z","timestamp":1766386893000},"page":"13","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glucosinolate\u2013Myrosinase Formulations for Adult Obesity: Towards Next Generation of Bioactive Therapies"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6358-3694","authenticated-orcid":false,"given":"Concepci\u00f3n","family":"Medrano-Padial","sequence":"first","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS, Campus Universitario de Espinardo, 25, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7442-7875","authenticated-orcid":false,"given":"Cassidy Bo","family":"Harris","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS, Campus Universitario de Espinardo, 25, 30100 Murcia, Spain"}]},{"given":"Ver\u00f3nica","family":"Mellado-Romero","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS, Campus Universitario de Espinardo, 25, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-712X","authenticated-orcid":false,"given":"Ra\u00fal","family":"Dom\u00ednguez-Perles","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS, Campus Universitario de Espinardo, 25, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6547-8764","authenticated-orcid":false,"given":"Diego A.","family":"Moreno","sequence":"additional","affiliation":[{"name":"Laboratorio de Fitoqu\u00edmica y Alimentos Saludables (LabFAS), CSIC, CEBAS, Campus Universitario de Espinardo, 25, 30100 Murcia, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"ref_1","unstructured":"(2025, January 11). 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