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compound (PP) was recently found to be a promising anticancer agent for colorectal and breast cancers. However, this compound exhibited low selectivity and poor water solubility. To address these challenges, albumin gel nanoparticles were used, where the gel matrix is formed by cross-linking of BSA molecules, allowing for a high concentration of this hydrophobic drug to be carried with no cytotoxicity to non-tumor cells. Functionalization with hyaluronic acid (HA) was employed to target CD44-overexpressing cancer cells, specifically triple-negative breast cancer (MDA-MB-231) and colorectal cancer cell lines (HCT 116). The gel nanoparticles present mean sizes below 250 nm, very low polydispersity, small aggregation tendency, and excellent colloidal stability in PBS buffer for a storage period of 30 days. Moreover, the drug-loaded particles showed high encapsulation efficiencies (above 85%) and sustained release profiles. Drug-loaded BSA\/HA particles (PP-HA-BSA-NPs) revealed advantageous activity, presenting around 55% and 23% cell viability at a IC50 drug concentration for triple-negative breast cancer (the most aggressive breast cancer subtype) and colorectal cancer (second leading cause of cancer-related deaths), respectively. In conclusion, these nanoparticles outperform the ones without HA, demonstrating target capabilities, while retaining the drug\u2019s anticancer activity and reducing the drug\u2019s toxicity. These results are promising for future in vivo assays and clinical translational applications.<\/jats:p>","DOI":"10.3390\/gels11090759","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:08:03Z","timestamp":1758542883000},"page":"759","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Albumin\/Hyaluronic Acid Gel Nanoparticles Loaded with a Pyrimidine-Based Drug for Potent Anticancer Activity"],"prefix":"10.3390","volume":"11","author":[{"given":"Sofia","family":"Teixeira","sequence":"first","affiliation":[{"name":"Centre of Chemistry of University of Minho (CQUM), Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Physics Centre of Minho and Porto Universities (CF-UM-UP) and LaPMET (Laboratory of Physics for Materials and Emergent Technologies), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0937-4047","authenticated-orcid":false,"given":"D\u00e9bora","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associated Laboratory, 4800 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9265-0630","authenticated-orcid":false,"given":"Ligia R.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associated Laboratory, 4800 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8666-0362","authenticated-orcid":false,"given":"M. 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Rev."},{"key":"ref_6","unstructured":"Anwar, U., Sattar, A., Rasheed, M.A., Shabbir, M.A.B., and Abbas, M. (2025). Preparation, characterization and toxicological evaluation of azithromycin-loaded chitosan nanoparticles alone and in combination with cetirizine dihydrochloride. Asian J. Agric. Biol., 2025."},{"key":"ref_7","first-page":"742","article-title":"Anticancer Activity of the 28.4 kDa Protein from Pediococcus pentosaceus SR6 in MCF-7 Breast Cancer Cell Line","volume":"13","author":"Suardana","year":"2024","journal-title":"Int. J. Vet. Sci."},{"key":"ref_8","first-page":"1","article-title":"Biogenic synthesis and characterization of silver nanoparticles: Exploring antioxidant and anti-Inflammatory activities and assessing antimicrobial potential against multidrug-resistant bacteria","volume":"2024","author":"Rasool","year":"2024","journal-title":"Asian J. Agric. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Liu, G., Yang, L., Chen, G., Xu, F., Yang, F., Yu, H., Li, L., Dong, X., Han, J., and Cao, C. (2021). A Review on Drug Delivery System for Tumor Therapy. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.735446"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/s41392-017-0004-3","article-title":"Controlled drug delivery vehicles for cancer treatment and their performance","volume":"3","author":"Senapati","year":"2018","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6945","DOI":"10.2147\/IJN.S467876","article-title":"Albumin Nanoparticle-Based Drug Delivery Systems","volume":"19","author":"Qu","year":"2024","journal-title":"Int. J. Nanomed."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"21","DOI":"10.2147\/NSA.S441324","article-title":"Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method","volume":"17","author":"Tanjung","year":"2024","journal-title":"Nanotechnol. Sci. Appl."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1016\/j.drudis.2018.01.051","article-title":"Navigating albumin-based nanoparticles through various drug delivery routes","volume":"23","author":"Tan","year":"2018","journal-title":"Drug Discov. Today"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Prajapati, R., Garcia-Garrido, E., and Somoza, \u00c1. (2021). Albumin-Based Nanoparticles for the Delivery of Doxorubicin in Breast Cancer. Cancers, 13.","DOI":"10.3390\/cancers13123011"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"107601","DOI":"10.1016\/j.jmgm.2020.107601","article-title":"Exploring how structural and dynamic properties of bovine and canine serum albumins differ from human serum albumin","volume":"98","author":"Ketrat","year":"2020","journal-title":"J. Mol. Graph. Model."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1166\/sam.2021.4128","article-title":"Preparation of Metformin\/Doxorubicin-Bovine Serum Albumin-Hyaluronic Acid-Carbon Dots Nanoparticles and Their Application in Treating Polycystic Ovary Syndrome on Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor of Patient Combine with Me","volume":"13","author":"Cheng","year":"2021","journal-title":"Sci. Adv. Mater."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Vismara, E., Bongio, C., Coletti, A., Edelman, R., Serafini, A., Mauri, M., Simonutti, R., Bertini, S., Urso, E., and Assaraf, Y. (2017). Albumin and Hyaluronic Acid-Coated Superparamagnetic Iron Oxide Nanoparticles Loaded with Paclitaxel for Biomedical Applications. Molecules, 22.","DOI":"10.3390\/molecules22071030"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1208\/s12249-008-9146-5","article-title":"5-Fluorouracil-Loaded BSA Nanoparticles: Formulation Optimization and In Vitro Release Study","volume":"9","author":"Maghsoudi","year":"2008","journal-title":"AAPS Pharmscitech"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Tincu Iurciuc, C.-E., Andri\u021boiu, C.V., Popa, M., and Ochiuz, L. (2023). Recent Advancements and Strategies for Overcoming the Blood\u2013Brain Barrier Using Albumin-Based Drug Delivery Systems to Treat Brain Cancer, with a Focus on Glioblastoma. Polymers, 15.","DOI":"10.3390\/polym15193969"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.jconrel.2021.01.033","article-title":"Hyaluronic acid and albumin based nanoparticles for drug delivery","volume":"331","author":"Lei","year":"2021","journal-title":"J. Control. Release"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Teixeira, S., Carvalho, M.A., and Castanheira, E.M.S. (2022). Functionalized Liposome and Albumin-Based Systems as Carriers for Poorly Water-Soluble Anticancer Drugs: An Updated Review. Biomedicines, 10.","DOI":"10.3390\/biomedicines10020486"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s13045-018-0605-5","article-title":"The biology and role of CD44 in cancer progression: Therapeutic implications","volume":"11","author":"Chen","year":"2018","journal-title":"J. Hematol. Oncol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Hassn Mesrati, M., Syafruddin, S.E., Mohtar, M.A., and Syahir, A. (2021). CD44: A Multifunctional Mediator of Cancer Progression. Biomolecules, 11.","DOI":"10.3390\/biom11121850"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Vadhan, A., Hou, M.-F., Vijayaraghavan, P., Wu, Y.-C., Hu, S.C.-S., Wang, Y.-M., Cheng, T.-L., Wang, Y.-Y., and Yuan, S.-S.F. (2022). CD44 Promotes Breast Cancer Metastasis through AKT-Mediated Downregulation of Nuclear FOXA2. Biomedicines, 10.","DOI":"10.3390\/biomedicines10102488"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wang, Z., Tang, Y., Xie, L., Huang, A., Xue, C., Gu, Z., Wang, K., and Zong, S. (2019). The Prognostic and Clinical Value of CD44 in Colorectal Cancer: A Meta-Analysis. Front. Oncol., 9.","DOI":"10.3389\/fonc.2019.00309"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Nabil, G., Alzhrani, R., Alsaab, H., Atef, M., Sau, S., Iyer, A., and Banna, H. (2021). CD44 Targeted Nanomaterials for Treatment of Triple-Negative Breast Cancer. Cancers, 13.","DOI":"10.3390\/cancers13040898"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7657","DOI":"10.18632\/oncotarget.7084","article-title":"Role of CD44high\/CD133high HCT-116 cells in the tumorigenesis of colon cancer","volume":"7","author":"Zhou","year":"2016","journal-title":"Oncotarget"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"119127","DOI":"10.1016\/j.ijpharm.2020.119127","article-title":"Applications and delivery mechanisms of hyaluronic acid used for topical\/transdermal delivery\u2014A review","volume":"578","author":"Zhu","year":"2020","journal-title":"Int. J. Pharm."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Teixeira, S., Ferreira, D., Rodrigues, A.R.O., Rodrigues, L.R., Castanheira, E.M.S., and Carvalho, M.A. (2025). Liposomal Formulations for Efficient Delivery of a Novel, Highly Potent Pyrimidine-Based Anticancer Drug. Pharmaceuticals, 18.","DOI":"10.3390\/ph18081210"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Peters Jr., T. (1995). All About Albumin: Biochemistry, Genetics, and Medical Applications, Academic Press Inc.","DOI":"10.1016\/B978-012552110-9\/50006-4"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.ijbiomac.2016.05.032","article-title":"A simple improved desolvation method for the rapid preparation of albumin nanoparticles","volume":"91","author":"Dastmalchi","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1021\/acs.chemrev.0c00779","article-title":"The Use of Alternative Strategies for Enhanced Nanoparticle Delivery to Solid Tumors","volume":"121","author":"Izci","year":"2021","journal-title":"Chem. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1515\/abm-2020-0032","article-title":"Albumin and functionalized albumin nanoparticles: Production strategies, characterization, and target indications","volume":"14","author":"Srivastava","year":"2020","journal-title":"Asian Biomed."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1016\/j.xphs.2020.07.015","article-title":"Freeze-Drying of Pharmaceutical and Nutraceutical Nanoparticles: The Effects of Formulation and Technique Parameters on Nanoparticles Characteristics","volume":"109","author":"Mohammady","year":"2020","journal-title":"J. Pharm. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.ijbiomac.2019.02.005","article-title":"Chitosan coated human serum albumin nanoparticles: A promising strategy for nose-to-brain drug delivery","volume":"129","author":"Piazzini","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.ijpharm.2008.07.004","article-title":"Freeze drying of human serum albumin (HSA) nanoparticles with different excipients","volume":"363","author":"Anhorn","year":"2008","journal-title":"Int. J. Pharm."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1021\/ja02086a003","article-title":"The rate of solution of solid substances in their own solutions","volume":"19","author":"Noyes","year":"1897","journal-title":"J. Am. Chem. Soc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ijpharm.2005.10.044","article-title":"On the use of the Weibull function for the discernment of drug release mechanisms","volume":"309","author":"Papadopoulou","year":"2006","journal-title":"Int. J. Pharm."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0168-3659(87)90035-6","article-title":"A simple equation for description of solute release II. Fickian and anomalous release from swellable devices","volume":"5","author":"Ritger","year":"1987","journal-title":"J. Control. Release"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Li, Y., Shi, S., Ming, Y., Wang, L., Li, C., Luo, M., Li, Z., Li, B., and Chen, J. (2018). Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting. J. Nanobiotechnol., 16.","DOI":"10.1186\/s12951-018-0424-4"},{"key":"ref_41","unstructured":"(2009). Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity (Standard No. ISO 10993-5:2009)."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"34956","DOI":"10.1039\/C5RA01683E","article-title":"The quantitative detection of the uptake and intracellular fate of albumin nanoparticles","volume":"5","author":"Jiang","year":"2015","journal-title":"RSC Adv."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jconrel.2020.03.004","article-title":"FcRn overexpression in human cancer drives albumin recycling and cell growth; a mechanistic basis for exploitation in targeted albumin-drug designs","volume":"322","author":"Larsen","year":"2020","journal-title":"J. Control. Release"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.jconrel.2021.07.007","article-title":"FcRn expression in cancer: Mechanistic basis and therapeutic opportunities","volume":"337","author":"Howard","year":"2021","journal-title":"J. Control. Release"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jconrel.2016.07.008","article-title":"Oral delivery of anti-MDM2 inhibitor SP141-loaded FcRn-targeted nanoparticles to treat breast cancer and metastasis","volume":"237","author":"Qin","year":"2016","journal-title":"J. Control. Release"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"24337","DOI":"10.18632\/oncotarget.15363","article-title":"Hyaluronic acid-serum albumin conjugate-based nanoparticles for targeted cancer therapy","volume":"8","author":"Edelman","year":"2017","journal-title":"Oncotarget"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0945-053X(01)00186-X","article-title":"CD44-mediated uptake and degradation of hyaluronan","volume":"21","author":"Knudson","year":"2002","journal-title":"Matrix Biol."},{"key":"ref_48","unstructured":"(2024, December 01). Created with Biorender. Available online: https:\/\/biorender.com\/."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"104501","DOI":"10.1016\/j.jddst.2023.104501","article-title":"Synthesis, characterization, in-silico and in-vitro anticancer studies of Plumbagin encapsulated albumin nanoparticles for breast cancer treatment","volume":"84","author":"Solanki","year":"2023","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Senra, S.F.M., Veloso, S.R.S., Lira, M., and Castanheira, E.M.S. (2025). Albumin\/Hyaluronic Acid Nanoparticle-Laden Contact Lenses for the Ocular Delivery of 5-Fluorouracil. J. Biomed. Mater. Res. 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