{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T05:43:48Z","timestamp":1771307028893,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["UIDB\/00195\/2020"],"award-info":[{"award-number":["UIDB\/00195\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>The textile industry stands as a prominent contributor to global environmental pollution, primarily attributable to its extensive reliance on synthetic dyes, hazardous components, and solvents throughout the textile dyeing and treatment processes. Consequently, the pursuit of sustainable textile solutions becomes imperative, aimed at replacing these environmentally unfriendly constituents with biobased and bioactive pigments, antibacterial agents, and, notably, natural solvents. Achieving this goal is a formidable yet indispensable challenge. In this study, the dyeing ability of the crude gel prodigiosin, produced by non-pathogenic bacteria Serratia plymuthica, was investigated on various multifiber fabrics at different conditions (temperature and pH) and by using salts and alternative mordants (the conventional Ferrous Sulphate (FeSO4) and a new bio-mordant, L-Cysteine (L-Cys)). Additionally, a novel gel-based Choline chloride (ChCl)\/Lactic acid (LA) (1:2) deep eutectic solvent (DES) dyeing medium was studied to replace the organic solvents. Nylon fabrics dyed with 3.0% over the weight of the fiber (owf) L-Cys at pH = 8.3 had improved color fastness to washing, while the gel-based ChCl\/LA (1:2) DES dyebath provided a better color fastness to light. Moreover, nylon fabrics under these conditions exhibited remarkable antimicrobial activity against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). In conclusion, the utilization of the crude gel-based prodigiosin pigment demonstrates a distinct advantage in dyeing textile materials, aligning with the growing consumer demand for more eco-friendly and sustainable products. Additionally, the application of the natural reducing agent L-Cys, previously untested as a bio-mordant, in conjunction with the use of gel-based DES as a dyeing medium, has showcased improved colorimetric and antibacterial properties when applied to nylon that is dyed with the crude gel prodigiosin pigment.<\/jats:p>","DOI":"10.3390\/gels9100800","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T09:14:22Z","timestamp":1696497262000},"page":"800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Sustainable Bioactive Dyeing of Textiles: A Novel Strategy Using Bacterial Pigments, Natural Antibacterial Ingredients, and Deep Eutectic Solvents"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2148-9602","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Mouro","sequence":"first","affiliation":[{"name":"FibEnTech Research Unit, Faculty of Engineering, University of Beira Interior, 6200-001 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3332-834X","authenticated-orcid":false,"given":"Ana P.","family":"Gomes","sequence":"additional","affiliation":[{"name":"FibEnTech Research Unit, Faculty of Engineering, University of Beira Interior, 6200-001 Covilh\u00e3, Portugal"}]},{"given":"Rita V.","family":"Costa","sequence":"additional","affiliation":[{"name":"FibEnTech Research Unit, Faculty of Engineering, University of Beira Interior, 6200-001 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5988-6280","authenticated-orcid":false,"given":"Farzaneh","family":"Moghtader","sequence":"additional","affiliation":[{"name":"FibEnTech Research Unit, Faculty of Engineering, University of Beira Interior, 6200-001 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3290-9529","authenticated-orcid":false,"given":"Isabel C.","family":"Gouveia","sequence":"additional","affiliation":[{"name":"FibEnTech Research Unit, Faculty of Engineering, University of Beira Interior, 6200-001 Covilh\u00e3, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121866","DOI":"10.1016\/j.jclepro.2020.121866","article-title":"Cleaner Dyeing of Textiles Using Plasma Treatment and Natural Dyes: A Review","volume":"265","author":"Haji","year":"2020","journal-title":"J. Clean. Prod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"252","DOI":"10.3390\/textiles2020013","article-title":"Bacterial Secondary Metabolites as Biopigments for Textile Dyeing","volume":"2","author":"Kramar","year":"2022","journal-title":"Textiles"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.ejar.2021.05.004","article-title":"Antimicrobial Activity of Textile Fabrics Dyed with Prodigiosin Pigment Extracted from Marine Serratia rubidaea RAM_Alex Bacteria","volume":"47","author":"Metwally","year":"2021","journal-title":"Egypt. J. Aquat. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107953","DOI":"10.1016\/j.dyepig.2019.107953","article-title":"Natural Dye from Croton urucurana Baill. Bark: Extraction, Physicochemical Characterization, Textile Dyeing and Color Fastness Properties","volume":"173","author":"Silva","year":"2020","journal-title":"Dyes Pigm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"112303","DOI":"10.1016\/j.indcrop.2020.112303","article-title":"Dyeing of Textiles with Natural Dyes Extracted from Terminalia arjuna and Thespesia populnea Fruits","volume":"148","author":"Amutha","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/cote.12673","article-title":"A Novel and Eco-Friendly Approach for Dyeing Polyester Fabrics by Liquid Disperse Dyes Treated with Deep Eutectic Solvent","volume":"139","author":"Zheng","year":"2023","journal-title":"Color. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.1039\/D2GC01292H","article-title":"An Innovative, Low-Cost and Environment-Friendly Approach by Using a Deep Eutectic Solvent as the Water Substitute to Minimize Waste in the Textile Industry and for Better Clothing Performance","volume":"24","author":"Jiang","year":"2022","journal-title":"Green Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.ibiod.2016.01.024","article-title":"Utilization of Agro-Industrial Waste for the Production of Yellowish-Orange Pigment from Chryseobacterium artocarpi CECT 8497","volume":"113","author":"Aruldass","year":"2016","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.cofs.2014.12.002","article-title":"Production of Microbial Pigments Utilizing Agro-Industrial Waste: A Review","volume":"1","author":"Panesar","year":"2015","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_10","unstructured":"Venil, C.K., Devi, P.R., and Ahmad, W.A. (2020). Valorisation of Agro-Industrial Residues\u2014Volume I: Biological Approaches, Springer."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Amorim, L.F.A., Fangueiro, R., and Gouveia, I.C. (2022). Characterization of Bioactive Colored Materials Produced from Bacterial Cellulose and Bacterial Pigments. Materials, 15.","DOI":"10.3390\/ma15062069"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Amorim, L.F.A., Mouro, C., Riool, M., and Gouveia, I.C. (2022). Antimicrobial Food Packaging Based on Prodigiosin-Incorporated Double-Layered Bacterial Cellulose and Chitosan Composites. Polymers, 14.","DOI":"10.3390\/polym14020315"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1007\/s13197-018-3188-9","article-title":"Antioxidant and Antimicrobial Activity of Bioactive Prodigiosin Produces from Serratia marcescens Using Agricultural Waste as a Substrate","volume":"55","author":"Arivizhivendhan","year":"2018","journal-title":"J. Food Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bhagwat, A., and Padalia, U. (2020). Optimization of Prodigiosin Biosynthesis by Serratia marcescens Using Unconventional Bioresources. J. Genet. Eng. Biotechnol., 18.","DOI":"10.1186\/s43141-020-00045-7"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Elkenawy, N.M., Yassin, A.S., Elhifnawy, H.N., and Amin, M.A. (2017). Optimization of Prodigiosin Production by Serratia marcescens Using Crude Glycerol and Enhancing Production Using Gamma Radiation. Biotechnol. Rep., 14.","DOI":"10.1016\/j.btre.2017.04.001"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lazic, J., Skaro Bogojevic, S., Vojnovic, S., Aleksic, I., Milivojevic, D., Kretzschmar, M., Gulder, T., Petkovic, M., and Nikodinovic-Runic, J. (2022). Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117. Molecules, 27.","DOI":"10.3390\/molecules27123729"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Nguyen, S.L.T., Nguyen, T.C., Do, T.T., Vu, T.L., Nguyen, T.T., Do, T.T., Nguyen, T.H.T., Le, T.H., Trinh, D.K., and Nguyen, T.A.T. (2022). Study on the Anticancer Activity of Prodigiosin from Variants of Serratia marcescens QBN VTCC 910026. Biomed. Res. Int., 2022.","DOI":"10.1155\/2022\/4053074"},{"key":"ref_18","first-page":"254","article-title":"Comparative Studies on Prodigiosin Production by Serratia marcescens Using Various Crude Fatty Acid Sources-Its Characterization and Applications","volume":"2","author":"Picha","year":"2015","journal-title":"Int. J. Curr. Microbiol. Appl. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.dyepig.2016.11.043","article-title":"Dyeing and Antibacterial Properties of Cotton Dyed with Prodigiosins Nanomicelles Produced by Microbial Fermentation","volume":"138","author":"Ren","year":"2017","journal-title":"Dyes Pigm."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3390\/textiles1010003","article-title":"Extraction and Application of Pigment from Serratia marcescens SB08, an Insect Enteric Gut Bacterium, for Textile Dyeing","volume":"1","author":"Venil","year":"2021","journal-title":"Textiles"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1590\/fst.35719","article-title":"Structure of Prodigiosin from Serratia marcescens NJZT-1 and Its Cytotoxicity on TSC2-Null Cells","volume":"41","author":"Zhao","year":"2020","journal-title":"Food Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Nguyen, T.H., Wang, S.L., and Nguyen, V.B. (2022). Recent Advances in Eco-Friendly and Scaling-Up Bioproduction of Prodigiosin and Its Potential Applications in Agriculture. Agronomy, 12.","DOI":"10.3390\/agronomy12123099"},{"key":"ref_23","unstructured":"Liu, X., Shen, J., Wang, Y., Wu, J., and Lu, Z. (2011). Biological Dye Containing Prodigiosins, Preparation Method Thereof and Application Thereof. (CN102199366A)."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1021\/bp070481r","article-title":"Antibacterial Colorants: Characterization of Prodiginines and Their Applications on Textile Materials","volume":"24","author":"Alihosseini","year":"2008","journal-title":"Biotechnol. Prog."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1007\/s11274-014-1644-x","article-title":"Crude Bacterial Extracts of Two New Streptomyces sp. Isolates as Bio-Colorants for Textile Dyeing","volume":"30","author":"Kramar","year":"2014","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/10826068.2012.721850","article-title":"Mutant Breeding of Serratia marcescens Strain for Enhancing Prodigiosin Production and Application to Textiles","volume":"43","author":"Liu","year":"2013","journal-title":"Prep. Biochem. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s12010-011-9427-4","article-title":"Production, Partial Characterization, and Use of a Red Biochrome Produced by Serratia sakuensis subsp. Nov Strain KRED for Dyeing Natural Fibers","volume":"166","author":"Vaidyanathan","year":"2012","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_28","first-page":"116","article-title":"Production and Characterization of Crude and Encapsulated Prodigiosin Pigment","volume":"4","author":"Namazkar","year":"2013","journal-title":"Int. J. Chem. Sci. Appl."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.dyepig.2008.05.008","article-title":"The Mordant Dyeing of Wool Using Tannic Acid and FeSO4, Part 1: Initial Findings","volume":"80","author":"Burkinshaw","year":"2009","journal-title":"Dyes Pigm."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1080\/00405001003722369","article-title":"UV Protection Properties of Silk Fabric Dyed with Eucalyptus Leaf Extract","volume":"102","author":"Mongkholrattanasit","year":"2011","journal-title":"J. Text. Inst."},{"key":"ref_31","first-page":"405626","article-title":"Effects of Different Mordants on Silk Fabric Dyed with Onion Outer Skin Extracts","volume":"2014","author":"Uddin","year":"2014","journal-title":"J. Text."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"115723","DOI":"10.1016\/j.seppur.2019.115723","article-title":"Deep Eutectic Solvents Used as the Green Media for the Efficient Extraction of Caffeine from Chinese Dark Tea","volume":"227","author":"Cai","year":"2019","journal-title":"Sep. Purif. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Torres-Vega, J., G\u00f3mez-Alonso, S., Pastene-Navarrete, E., and P\u00e9rez-Navarro, J. (2020). Green Extraction of Alkaloids and Polyphenols from Peumus boldus Leaves with Natural Deep Eutectic Solvents and Profiling by HPLC-PDA-IT-MS\/MS and HPLC-QTOF-MS\/MS. Plants, 9.","DOI":"10.3390\/plants9020242"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jep.2018.08.040","article-title":"Evaluation of the Influence of Green Extraction Solvents on the Cytotoxic Activities of Crinum (Amaryllidaeae) Alkaloid Extracts Using in-Vitro-in-Silico Approach","volume":"227","author":"Shawky","year":"2018","journal-title":"J. Ethnopharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1002\/jps.20815","article-title":"Protect from Light: Photodegradation and Protein Biologics","volume":"96","author":"Kerwin","year":"2007","journal-title":"J. Pharm. Sci."},{"key":"ref_36","first-page":"383","article-title":"Der Einfluss Des Lichtes Und Ionisierender Strahlung Auf Die Autoxydation von Cystein Und Die Reaktion Zwischen Cystein Und 3, 4-Benzpyren","volume":"2","author":"Koch","year":"1960","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.jclepro.2017.01.168","article-title":"Dyeing and Functional Properties of Polyester Fabric Dyed with Prodigiosins Nanomicelles Produced by Microbial Fermentation","volume":"148","author":"Ren","year":"2017","journal-title":"J. Clean. Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.jbiotec.2010.09.387","article-title":"Nanobiotechnology: A New Strategy to Develop Non-Toxic Antimicrobial Textiles for Healthcare Applications","volume":"150","author":"Gouveia","year":"2010","journal-title":"J. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mouro, C., Martins, R., Gomes, A.P., and Gouveia, I.C. (2023). Upcycling Wool Waste into Keratin Gel-Based Nanofibers Using Deep Eutectic Solvents. Gels, 9.","DOI":"10.20944\/preprints202307.1529.v1"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Akbar, N., Khan, N.A., Ibrahim, T., Khamis, M., Khan, A.S., Alharbi, A.M., Alfahemi, H., and Siddiqui, R. (2023). Antimicrobial Activity of Novel Deep Eutectic Solvents. Sci. Pharm., 91.","DOI":"10.3390\/scipharm91010009"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1111\/1541-4337.13087","article-title":"Wool Keratin as a Novel Alternative Protein: A Comprehensive Review of Extraction, Purification, Nutrition, Safety, and Food Applications","volume":"22","author":"Giteru","year":"2023","journal-title":"Compr. Rev. Food Sci. Food Saf."}],"container-title":["Gels"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2310-2861\/9\/10\/800\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:01:30Z","timestamp":1760130090000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2310-2861\/9\/10\/800"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,5]]},"references-count":41,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["gels9100800"],"URL":"https:\/\/doi.org\/10.3390\/gels9100800","relation":{},"ISSN":["2310-2861"],"issn-type":[{"value":"2310-2861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,5]]}}}