{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T06:47:48Z","timestamp":1772952468196,"version":"3.50.1"},"reference-count":119,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T00:00:00Z","timestamp":1586908800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/132215\/2017"],"award-info":[{"award-number":["SFRH\/BD\/132215\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/05183\/2020"],"award-info":[{"award-number":["UIDB\/05183\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union\u2019s H2020 RIA program","award":["634476"],"award-info":[{"award-number":["634476"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>When compared to modern lean-type breeds, Portuguese local Alentejano (AL) and B\u00edsaro (BI) pig breeds present a high potential for subcutaneous and intramuscular fat (IMF) deposition which contributes for better meat quality. The aim of this work was to explore the genome function to better understand the underlying physiological mechanisms associated with body fat accretion. Dorsal subcutaneous fat samples were collected at slaughter from adult animals (n = 4 for each breed) with ~150 kg body weight. Total RNA was obtained and sequenced for transcriptome analysis using DESeq2. A total of 458 differentially expressed (DE) genes (q-value &lt; 0.05) were identified, with 263 overexpressed in AL and 195 in BI. Key genes involved in de novo fatty acid biosynthesis, elongation and desaturation were upregulated in AL such as ACLY, FASN, ME1, ELOVL6 and SCD. A functional enrichment analysis of the DE genes was performed using Ingenuity Pathway Analysis. Cholesterol synthesis is suggested to be higher in AL via SREBF2, SCAP and PPARG, while lipolytic activity may be more active in BI through GH and AMPK signalling. Increased signalling of CD40 together with the predicted activation of INSIG1 and INSIG2 in BI suggests that this breed is more sensitive to insulin whereas the AL is less sensitive like the Iberian breed.<\/jats:p>","DOI":"10.3390\/genes11040422","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T09:19:50Z","timestamp":1586942390000},"page":"422","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1189-9803","authenticated-orcid":false,"given":"Andr\u00e9","family":"Albuquerque","sequence":"first","affiliation":[{"name":"MED-Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investiga\u00e7\u00e3o e Forma\u00e7\u00e3o Avan\u00e7ada &amp; Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5738-8435","authenticated-orcid":false,"given":"Cristina","family":"\u00d3vilo","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA), 28040 Madrid, Spain"}]},{"given":"Yolanda","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA), 28040 Madrid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7459-2836","authenticated-orcid":false,"given":"Rita","family":"Ben\u00edtez","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA), 28040 Madrid, Spain"}]},{"given":"Adri\u00e1n","family":"L\u00f3pez-Garcia","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA), 28040 Madrid, Spain"}]},{"given":"Fabi\u00e1n","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA), 28040 Madrid, Spain"}]},{"given":"Maria do Ros\u00e1rio","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"MED &amp; Departamento de Fitotecnia, Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3900-5592","authenticated-orcid":false,"given":"Marta","family":"Laranjo","sequence":"additional","affiliation":[{"name":"MED-Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investiga\u00e7\u00e3o e Forma\u00e7\u00e3o Avan\u00e7ada &amp; Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3597-6746","authenticated-orcid":false,"given":"Rui","family":"Charneca","sequence":"additional","affiliation":[{"name":"MED &amp; Departamento de Medicina Veterin\u00e1ria, Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3616-8406","authenticated-orcid":false,"given":"Jos\u00e9 Manuel","family":"Martins","sequence":"additional","affiliation":[{"name":"MED &amp; Departamento de Zootecnia, Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.7150\/ijbs.3.153","article-title":"Genetic resources, genome mapping and evolutionary genomics of the pig (Sus scrofa)","volume":"3","author":"Chen","year":"2007","journal-title":"Int. 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