{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:53:53Z","timestamp":1760237633094,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["PDE 204792\/2018-5"],"award-info":[{"award-number":["PDE 204792\/2018-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["PDSE 88881.132814\/2016-01"],"award-info":[{"award-number":["PDSE 88881.132814\/2016-01"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/K008226\/1"],"award-info":[{"award-number":["BB\/K008226\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","award":["16\/2551-000485-7"],"award-info":[{"award-number":["16\/2551-000485-7"]}],"id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The Columbidae species (Aves, Columbiformes) show considerable variation in their diploid numbers (2n = 68\u201386), but there is limited understanding of the events that shaped the extant karyotypes. Hence, we performed whole chromosome painting (wcp) for paints GGA1-10 and bacterial artificial chromosome (BAC) probes for chromosomes GGA11-28 for Columbina passerina, Columbina talpacoti, Patagioenas cayennensis, Geotrygon violacea and Geotrygon montana. Streptopelia decaocto was only investigated with paints because BACs for GGA10-28 had been previously analyzed. We also performed phylogenetic analyses in order to trace the evolutionary history of this family in light of chromosomal changes using our wcp data with chicken probes and from Zenaida auriculata, Columbina picui, Columba livia and Leptotila verreauxi, previously published. G-banding was performed on all these species. Comparative chromosome paint and G-banding results suggested that at least one interchromosomal and many intrachromosomal rearrangements had occurred in the diversification of Columbidae species. On the other hand, a high degree of conservation of microchromosome organization was observed in these species. Our cladistic analysis, considering all the chromosome rearrangements detected, provided strong support for L. verreauxi and P. cayennensis, G. montana and G. violacea, C. passerina and C. talpacoti having sister taxa relationships, as well as for all Columbidae species analyzed herein. Additionally, the chromosome characters were mapped in a consensus phylogenetic topology previously proposed, revealing a pericentric inversion in the chromosome homologous to GGA4 in a chromosomal signature unique to small New World ground doves.<\/jats:p>","DOI":"10.3390\/genes11060632","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T06:34:16Z","timestamp":1591684456000},"page":"632","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes)"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6856-2152","authenticated-orcid":false,"given":"Rafael","family":"Kretschmer","sequence":"first","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK"},{"name":"Departamento de Gen\u00e9tica, Universidade Federal do Rio Grande do Sul, Porto Alegre 91509-900, Brazil"}]},{"given":"Ivanete de Oliveira","family":"Furo","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"},{"name":"Laborat\u00f3rio de Cultura de Tecidos e Citogen\u00e9tica, SAMAM, Instituto Evandro Chagas, Ananindeua 67030-000, Brazil"}]},{"given":"Anderson Jos\u00e9 Baia","family":"Gomes","sequence":"additional","affiliation":[{"name":"Instituto Federal do Par\u00e1, Abaetetuba 68440-000, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6498-0421","authenticated-orcid":false,"given":"Lucas G.","family":"Kiazim","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK"}]},{"given":"Ricardo Jos\u00e9","family":"Gunski","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diversidade Gen\u00e9tica Animal, Universidade Federal do Pampa, S\u00e3o Gabriel 97300-162, Brazil"}]},{"given":"Anal\u00eda del Valle","family":"Garnero","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diversidade Gen\u00e9tica Animal, Universidade Federal do Pampa, S\u00e3o Gabriel 97300-162, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1472-1613","authenticated-orcid":false,"given":"Jorge C.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9372-1381","authenticated-orcid":false,"given":"Malcolm A.","family":"Ferguson-Smith","sequence":"additional","affiliation":[{"name":"Cambridge Resource Centre for Comparative Genomics, University of Cambridge Department of Veterinary Medicine, Cambridge CB3 0ES, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6315-3352","authenticated-orcid":false,"given":"Edivaldo Herculano Corr\u00eaa","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Cultura de Tecidos e Citogen\u00e9tica, SAMAM, Instituto Evandro Chagas, Ananindeua 67030-000, Brazil"},{"name":"Instituto de Ci\u00eancias Exatas e Naturais, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7595-3226","authenticated-orcid":false,"given":"Darren K.","family":"Griffin","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1019-9303","authenticated-orcid":false,"given":"Thales Renato Ochotorena","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica, Universidade Federal do Rio Grande do Sul, Porto Alegre 91509-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4270-970X","authenticated-orcid":false,"given":"Rebecca E.","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1601-5223.1981.tb01757.x","article-title":"Chromosomes in birds (Aves): Evolutionary implications of macro- and microchromosome numbers and lengths","volume":"94","author":"Tegelstrom","year":"1981","journal-title":"Hereditas"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kretschmer, R., Ferguson-Smith, M.A., and de Oliveira, E.H.C. (2018). Karyotype evolution in birds: From conventional staining to chromosome painting. Genes, 9.","DOI":"10.3390\/genes9040181"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1038\/nature03154","article-title":"Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution","volume":"432","author":"Hillier","year":"2004","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1038\/s41467-018-04267-9","article-title":"Reconstruction of the diapsid ancestral genome permits chromosome evolution tracing in avian and non-avian dinosaurs","volume":"9","author":"Romanov","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2442","DOI":"10.1093\/gbe\/evw166","article-title":"Novel Insights Into Chromosome Evolution in Birds, Archosaurs, and Reptiles","volume":"8","author":"Narayan","year":"2016","journal-title":"Genome Biol. Evol."},{"key":"ref_6","unstructured":"Gibbs, D., Barnes, E., and Cox, J.D. (2001). Pigeons and Doves: A Guide to the Pigeons and Doves of the World, Pica Press."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1080\/10635150701549672","article-title":"Mitochondrial and nuclear DNA sequences support a cretaceous origin of Columbiformes and a dispersal driven radiation in the paleogene","volume":"56","author":"Pereira","year":"2007","journal-title":"Syst. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Soares, A.E.R., Novak, B.J., Haile, J., Heupink, T.H., Fjelds\u00e5, J., Gilbert, M.T.P., Poinar, H., Church, G.M., and Shapiro, B. (2016). Complete mitochondrial genomes of living and extinct pigeons revise the timing of the columbiform radiation. BMC Evol. Biol., 16.","DOI":"10.1186\/s12862-016-0800-3"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sibley, C.G., and Ahlquist, J.E. (1990). Phylogeny and Classification of Birds: A Study in Molecular Evolution, Yale University Press.","DOI":"10.2307\/j.ctt1xp3v3r"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1126\/science.295.5560.1683","article-title":"Flight of the dodo","volume":"295","author":"Shapiro","year":"2002","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/00087114.1976.10796650","article-title":"Chromosomal evolution in Columbiformes (Aves)","volume":"29","year":"1976","journal-title":"Caryologia"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF00056434","article-title":"Chromosomal evolution of South American Columbiformes (Aves)","volume":"62","year":"1984","journal-title":"Genetica"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1159\/000076309","article-title":"Comparative chromosome painting of chicken autosomal paints 1\u20139 in nine different bird species","volume":"103","author":"Guttenbach","year":"2003","journal-title":"Cytogenet. Genome Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1023\/B:CHRO.0000045779.50641.00","article-title":"High chromosome conservation detected by comparative chromosome painting in chicken, pigeon and passerine birds","volume":"12","author":"Derjusheva","year":"2004","journal-title":"Chromosome Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1590\/1678-4685-gmb-2017-0048","article-title":"Repetitive DNAs and shrink genomes: A chromosomal analysis in nine Columbidae species (Aves, Columbiformes)","volume":"41","author":"Kretschmer","year":"2018","journal-title":"Genet. Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s10577-018-9580-5","article-title":"Comparative chromosome painting in Columbidae (Columbiformes) reinforces divergence in Passerea and Columbea","volume":"26","author":"Kretschmer","year":"2018","journal-title":"Chromosome Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00412-018-0685-6","article-title":"Patterns of microchromosome organization remain highly conserved throughout avian evolution","volume":"128","author":"Kiazim","year":"2019","journal-title":"Chromosoma"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1007\/BF02146680","article-title":"A feather pulp culture for avian chromosomes with notes on the chromosomes of the peafowl and the ostrich","volume":"24","author":"Sasaki","year":"1968","journal-title":"Experientia"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Joseph, S., O\u2019Connor, R.E., Al Mutery, A.F., Watson, M., Larkin, D.M., and Griffin, D.K. (2018). Chromosome Level Genome Assembly and Comparative Genomics between Three Falcon Species Reveals an Unusual Pattern of Genome Organisation. Diversity, 10.","DOI":"10.3390\/d10040113"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1007\/s00412-004-0292-6","article-title":"Viability of X-autosome translocations in mammals: An epigenomic hypothesis from a rodent case-study","volume":"113","author":"Dobigny","year":"2004","journal-title":"Chromosoma"},{"key":"ref_21","unstructured":"Swoford, D.L. (2002). PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods), Sinauer Associates. Version 4."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Degrandi, T.M., Barcelos, S.A., Costa, A.L., Garnero, A.D.V., Hass, I., and Gunski, R.J. (2020). Introducing the Bird Chromosome Database: An overview of cytogenetic studies on birds. Cytogenet. Genome Res., in press.","DOI":"10.1159\/000507768"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1159\/000078570","article-title":"Karyotypic evolution in the Galliformes: An examination of the process of karyotypic evolution by comparison of the molecular cytogenetic findings with the molecular phylogeny","volume":"106","author":"Shibusawa","year":"2004","journal-title":"Cytogenet. Genome Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s10577-007-1210-6","article-title":"Characterization of chromosome structures of Falconinae (Falconidae, Falconiformes, Aves) by chromosome painting and delineation of chromosome rearrangements during their differentiation","volume":"16","author":"Nishida","year":"2008","journal-title":"Chromosome Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1159\/000494707","article-title":"The Karyotype of the Hoatzin (Opisthocomus hoazin)\u2014A Phylogenetic Enigma of the Neornithes","volume":"156","author":"Furo","year":"2018","journal-title":"Cytogenet. Genome Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20122893","DOI":"10.1098\/rspb.2012.2893","article-title":"Behavioural changes and the adaptive diversification of pigeons and doves","volume":"280","author":"Lapiedra","year":"2013","journal-title":"Proc. R. Soc. B"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1186\/s13059-018-1550-x","article-title":"Chromosome-level assembly reveals extensive rearrangement in saker falcon and budgerigar, but not ostrich, genomes","volume":"19","author":"Joseph","year":"2018","journal-title":"Genome Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1159\/000322358","article-title":"Synteny conservation of chicken macrochromosomes 1\u201310 in different Avian lineages revealed by cross-species chromosome painting","volume":"132","author":"Nanda","year":"2011","journal-title":"Cytogenet. Genome Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1159\/000163093","article-title":"Comparative chromosome painting between chicken and spectacled owl (Pulsatrix perspicillata): Implications for chromosomal evolution in the Strigidae (Aves, Strigiformes)","volume":"122","author":"Nagamachi","year":"2008","journal-title":"Cytogenet. Genome Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1159\/000471782","article-title":"Chromosome Painting in Trogon s. surrucura (Aves, Trogoniformes) Reveals a Karyotype Derived by Chromosomal Fissions, Fusions, and Inversions","volume":"151","author":"Degrandi","year":"2017","journal-title":"Cytogenet. Genome Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1159\/000103164","article-title":"Chromosome repatterning in three representative parrots (Psittaciformes) inferred from comparative chromosome painting","volume":"117","author":"Nanda","year":"2007","journal-title":"Cytogenet. Genome Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.tree.2009.12.004","article-title":"Evolutionary stasis: The stable chromosomes of birds","volume":"25","author":"Ellegren","year":"2010","journal-title":"Trends Ecol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Nie, W., O\u2019Brien, P.C.M., Fu, B., Wang, J., Su, W., He, K., Bed\u2019Hom, B., Volobouev, V., Ferguson-Smith, M.A., and Dobigny, G. (2015). Multidirectional chromosome painting substantiates the occurrence of extensive genomic reshuffling within Accipitriformes. BMC Evol. Biol., 15.","DOI":"10.1186\/s12862-015-0484-0"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1642\/AUK-14-193.1","article-title":"Patterns of diversification in small New World ground doves are consistent with major geologic events","volume":"132","author":"Sweet","year":"2014","journal-title":"Auk"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1038\/nature08819","article-title":"The genome of a songbird","volume":"464","author":"Warren","year":"2010","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1101\/gr.213660.116","article-title":"Upgrading short-read animal genome assemblies to chromosome level using comparative genomics and a universal probe set","volume":"27","author":"Damas","year":"2017","journal-title":"Genome Res."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/11\/6\/632\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:36:53Z","timestamp":1760175413000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/11\/6\/632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,8]]},"references-count":36,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["genes11060632"],"URL":"https:\/\/doi.org\/10.3390\/genes11060632","relation":{},"ISSN":["2073-4425"],"issn-type":[{"type":"electronic","value":"2073-4425"}],"subject":[],"published":{"date-parts":[[2020,6,8]]}}}