{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T15:39:44Z","timestamp":1768837184233,"version":"3.49.0"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872579"],"award-info":[{"award-number":["31872579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"USM-Strategic Initiative-Ten Q1Q2","award":["311.PCCB.411954"],"award-info":[{"award-number":["311.PCCB.411954"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Elongation of very long-chain fatty acid (Elovl) proteins are key enzymes that catalyze the rate-limiting step in the fatty acid elongation pathway. The most recently discovered member of the Elovl family, Elovl8, has been proposed to be a fish-specific elongase with two gene paralogs described in teleosts. However, the biological functions of Elovl8 are still to be elucidated. In this study, we showed that in contrast to previous findings, elovl8 is not unique to teleosts, but displays a rather unique and ample phylogenetic distribution. For functional determination, we generated elovl8a (elovl8a\u2212\/\u2212) and elovl8b (elovl8b\u2212\/\u2212) zebrafish using CRISPR\/Cas9 technology. Fatty acid composition in vivo and zebrafish liver cell experiments suggest that the substrate preference of Elovl8 overlapped with other existing Elovl enzymes. Zebrafish Elovl8a could elongate the polyunsaturated fatty acids (PUFAs) C18:2n-6 and C18:3n-3 to C20:2n-6 and C20:3n-3, respectively. Along with PUFA, zebrafish Elovl8b also showed the capacity to elongate C18:0 and C20:1. Gene expression quantification suggests that Elovl8a and Elovl8b may play a potentially important role in fatty acid biosynthesis. Overall, our results provide novel insights into the function of Elovl8a and Elovl8b, representing additional fatty acid elongases not previously described in chordates.<\/jats:p>","DOI":"10.3390\/genes12081287","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T10:24:17Z","timestamp":1629714257000},"page":"1287","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Evolution and Functional Characteristics of the Novel elovl8 That Play Pivotal Roles in Fatty Acid Biosynthesis"],"prefix":"10.3390","volume":"12","author":[{"given":"Shouxiang","family":"Sun","sequence":"first","affiliation":[{"name":"Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Ministry of Education\/Hubei Provincial Engineering Laboratory for Pond Aquaculture, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China"}]},{"given":"Yumei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1947-0550","authenticated-orcid":false,"given":"Pei-Tian","family":"Goh","sequence":"additional","affiliation":[{"name":"Center for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas 11800, Penang, Malaysia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8597-4730","authenticated-orcid":false,"given":"M\u00f3nica","family":"Lopes-Marques","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Center of Marine and Environmental Research, University of Porto, 4099002 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7697-386X","authenticated-orcid":false,"given":"L. Filipe C.","family":"Castro","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Center of Marine and Environmental Research, University of Porto, 4099002 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences of the Univeristy of Porto (FCUP), 4099002 Porto, Portugal"}]},{"given":"\u00d3scar","family":"Monroig","sequence":"additional","affiliation":[{"name":"Instituto de Acuicultura Torre de la Sal (IATS-CSIC), Ribera de Cabanes, 12595 Castellon, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6654-7680","authenticated-orcid":false,"given":"Meng-Kiat","family":"Kuah","sequence":"additional","affiliation":[{"name":"Center for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas 11800, Penang, Malaysia"}]},{"given":"Xiaojuan","family":"Cao","sequence":"additional","affiliation":[{"name":"Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Ministry of Education\/Hubei Provincial Engineering Laboratory for Pond Aquaculture, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3014-442X","authenticated-orcid":false,"given":"Alexander Chong","family":"Shu-Chien","sequence":"additional","affiliation":[{"name":"Center for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas 11800, Penang, Malaysia"}]},{"given":"Jian","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Lab of Freshwater Animal Breeding, College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Ministry of Education\/Hubei Provincial Engineering Laboratory for Pond Aquaculture, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.plipres.2006.01.004","article-title":"Fatty acid elongases in mammals: Their regulation and roles in metabolism","volume":"45","author":"Jakobsson","year":"2006","journal-title":"Prog. 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