{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T16:20:48Z","timestamp":1776788448376,"version":"3.51.2"},"reference-count":77,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,21]],"date-time":"2023-01-21T00:00:00Z","timestamp":1674259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFD2401700"],"award-info":[{"award-number":["2022YFD2401700"]}]},{"name":"National Key Research and Development Program of China","award":["32172992"],"award-info":[{"award-number":["32172992"]}]},{"name":"National Natural Science Foundation of China","award":["2022YFD2401700"],"award-info":[{"award-number":["2022YFD2401700"]}]},{"name":"National Natural Science Foundation of China","award":["32172992"],"award-info":[{"award-number":["32172992"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Many marine invertebrate larvae undergo complex morphological and physiological changes during the planktonic\u2014benthic transition (a.k.a. metamorphosis). In this study, transcriptome analysis of different developmental stages was used to uncover the molecular mechanisms underpinning larval settlement and metamorphosis of the mussel, Mytilus coruscus. Analysis of highly upregulated differentially expressed genes (DEGs) at the pediveliger stage revealed enrichment of immune-related genes. The results may indicate that larvae co-opt molecules of the immune system to sense and respond to external chemical cues and neuroendocrine signaling pathways forecast and trigger the response. The upregulation of adhesive protein genes linked to byssal thread secretion indicates the anchoring capacity required for larval settlement arises prior to metamorphosis. The results of gene expression support a role for the immune and neuroendocrine systems in mussel metamorphosis and provide the basis for future studies to disentangle gene networks and the biology of this important lifecycle transformation.<\/jats:p>","DOI":"10.3390\/genes14020287","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T03:21:54Z","timestamp":1674616914000},"page":"287","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Stage-Specific Transcriptomes of the Mussel Mytilus coruscus Reveals the Developmental Program for the Planktonic to Benthic Transition"],"prefix":"10.3390","volume":"14","author":[{"given":"Yu-Qing","family":"Wang","sequence":"first","affiliation":[{"name":"International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"}]},{"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Aquatic Technology Promotion Station, Sanmen Rural Bureau, Taizhou 317199, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1567-8215","authenticated-orcid":false,"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"}]},{"given":"Lizhi","family":"Chen","sequence":"additional","affiliation":[{"name":"Aquatic Technology Promotion Station, Sanmen Rural Bureau, Taizhou 317199, China"}]},{"given":"Yue-Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"}]},{"given":"Xue","family":"Shi","sequence":"additional","affiliation":[{"name":"International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1366-0246","authenticated-orcid":false,"given":"Deborah M.","family":"Power","sequence":"additional","affiliation":[{"name":"International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Comparative Endocrinology and Integrative Biology, Centro de Ci\u00eancias do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Yi-Feng","family":"Li","sequence":"additional","affiliation":[{"name":"International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,21]]},"reference":[{"key":"ref_1","unstructured":"(2022). China Fishery Statistical Yearbook, China Agriculture Press. (In Chinese)."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s10126-007-9001-9","article-title":"Marine biofilms as mediators of colonization by marine macroorganisms: Implications for antifouling and aquaculture","volume":"9","author":"Qian","year":"2007","journal-title":"Mar. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev-marine-010213-135049","article-title":"Mussels as a model system for integrative ecomechanics","volume":"7","author":"Carrington","year":"2015","journal-title":"Ann. Rev. Mar. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"113347","DOI":"10.1016\/j.ygcen.2019.113347","article-title":"Thyroid hormone receptor: A new player in epinephrine-induced larval metamorphosis of the hard-shelled mussel","volume":"287","author":"Li","year":"2020","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1006\/scdb.2000.0197","article-title":"Why and how marine-invertebrate larvae metamorphose so fast","volume":"116","author":"Hadfield","year":"2000","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1146\/annurev-marine-120709-142753","article-title":"Biofilms and marine invertebrate larvae: What bacteria produce that larvae use to choose settlement sites","volume":"3","author":"Hadfield","year":"2011","journal-title":"Ann. Rev. Mar. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.aquaculture.2018.01.002","article-title":"Molluscan bivalve settlement and metamorphosis: Neuroendocrine inducers and morphogenetic responses","volume":"487","author":"Joyce","year":"2018","journal-title":"Aquaculture"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1080\/08927014.2013.764412","article-title":"Larval settlement and metamorphosis of the mussel Mytilus coruscus in response to monospecific bacterial biofilms","volume":"29","author":"Yang","year":"2013","journal-title":"Biofouling"},{"key":"ref_9","first-page":"484","article-title":"Control of oyster settlement and metamorphosis by endogenous and exogenous chemical cues","volume":"46","author":"Bonar","year":"1990","journal-title":"Bull. Mar. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1080\/08927014.2019.1685661","article-title":"An \u03b12-adrenergic receptor is involved in larval metamorphosis in the mussel Mytilus coruscus","volume":"35","author":"Liang","year":"2019","journal-title":"Biofouling"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.aquaculture.2012.08.040","article-title":"Molecular characterization and functional analysis of adrenergic like receptor during larval metamorphosis in Crassostrea angulata","volume":"366","author":"Yang","year":"2012","journal-title":"Aquaculture"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s12861-020-00232-2","article-title":"Bivalves are NO different: Nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas","volume":"20","author":"Vogeler","year":"2020","journal-title":"BMC Dev. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"jeb167478","DOI":"10.1242\/jeb.167478","article-title":"HSP90 regulates larval settlement of the bryozoan Bugula neritina through NO pathway","volume":"221","author":"Yang","year":"2018","journal-title":"J. Exp. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"356","DOI":"10.3389\/fmars.2020.00356","article-title":"Nitric oxide negatively regulates larval metamorphosis in hard-shelled mussel (Mytilus coruscus)","volume":"7","author":"Zhu","year":"2020","journal-title":"Front. Mar. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1186\/s12864-020-07066-z","article-title":"Proteome of larval metamorphosis induced by epinephrine in the Fujian oyster Crassostrea angulata","volume":"21","author":"Di","year":"2020","journal-title":"BMC Genom."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1007\/s10126-016-9691-y","article-title":"Developmental transcriptome analysis and identification of genes involved in larval metamorphosis of the razor clam, Sinonovacula constricta","volume":"18","author":"Niu","year":"2016","journal-title":"Mar. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4434","DOI":"10.1111\/j.1365-294X.2009.04371.x","article-title":"Carry-over effect of larval settlement cue on postlarval gene expression in the marine gastropod Haliotis asinina","volume":"18","author":"Williams","year":"2009","journal-title":"Mol. Ecol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/gigascience\/giab024","article-title":"Chromosome-level genome assembly of the hard-shelled mussel Mytilus coruscus, a widely distributed species from the temperate areas of East Asia","volume":"10","author":"Yang","year":"2021","journal-title":"Gigascience"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/1471-2105-12-323","article-title":"RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome","volume":"12","author":"Li","year":"2011","journal-title":"BMC Bioinform."},{"key":"ref_21","first-page":"550","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"W316","DOI":"10.1093\/nar\/gkr483","article-title":"KOBAS 2.0: A web server for annotation and identification of enriched pathways and diseases","volume":"39","author":"Xie","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.1093\/bioinformatics\/bti610","article-title":"Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research","volume":"21","author":"Conesa","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"19288","DOI":"10.1038\/s41598-021-98930-9","article-title":"Larval metamorphosis is inhibited by methimazole and propylthiouracil that reveals possible hormonal action in the mussel Mytilus coruscus","volume":"11","author":"Li","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"914283","DOI":"10.3389\/fmars.2022.914283","article-title":"Knockdown of two iodothyronine deiodinase genes inhibits epinephrine-induced larval metamorphosis of the hard-shelled mussel Mytilus coruscus","volume":"9","author":"Shi","year":"2022","journal-title":"Front. Mar. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1080\/08927010802340309","article-title":"Induction of metamorphosis of pediveliger larvae of the mussel Mytilus galloprovincialis Lamarck, 1819 using neuroactive compounds, KCl, NH4Cl and organic solvents","volume":"24","author":"Yang","year":"2008","journal-title":"Biofouling"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/08927014.2011.553717","article-title":"Larval metamorphosis of the mussel Mytilus galloprovincialis Lamarck, 1819 in response to neurotransmitter blockers and tetraethylammonium","volume":"27","author":"Yang","year":"2011","journal-title":"Biofouling"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.aquaculture.2013.02.039","article-title":"Effects of neuroactive compounds, ions and organic solvents on larval metamorphosis of the mussel Mytilus coruscus","volume":"396\u2013399","author":"Yang","year":"2013","journal-title":"Aquaculture"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.aquaculture.2014.02.019","article-title":"Effects of adrenoceptor compounds on larval metamorphosis of the mussel Mytilus coruscus","volume":"426\u2013427","author":"Yang","year":"2014","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.fsi.2013.04.044","article-title":"Immune responses during the larval stages of Mytilus galloprovincialis: Metamorphosis alters immunocompetence, body shape and behavior","volume":"35","author":"Balseiro","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cbpb.2018.08.008","article-title":"Developmental dynamics of myogenesis in Pacific oyster Crassostrea gigas","volume":"227","author":"Li","year":"2019","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/S0044-8486(03)00521-0","article-title":"Anti-infectious immune effectors in marine invertebrates: Potential tools for disease control in larviculture","volume":"227","year":"2003","journal-title":"Aquaculture"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.marenvres.2018.11.007","article-title":"Oyster disease in a changing environment: Decrypting the link between pathogen, microbiome and environment","volume":"143","author":"King","year":"2019","journal-title":"Mar. Environ. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4739","DOI":"10.1242\/dev.129.20.4739","article-title":"A molecular analysis of ascidian metamorphosis reveals activation of an innate immune response","volume":"129","author":"Davidson","year":"2002","journal-title":"Development"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Dobretsov, S., and Rittschof, D. (2020). Love atfirst taste: Induction of larval settlement by marine microbes. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21030731"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/s00227-007-0720-2","article-title":"Larval metamorphosis of the mussel Mytilus galloprovincialis in response to Alteromonas sp. 1: Evidence for two chemical cues?","volume":"152","author":"Bao","year":"2007","journal-title":"Mar. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"161","DOI":"10.3354\/meps260161","article-title":"Composition and density of bacterial biofilms determine larval settlement of the polychaete Hydroides elegans","volume":"260","author":"Huang","year":"2003","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1007\/s00442-006-0470-8","article-title":"Larval settlement of the common Australian sea urchin Heliocidaris erythrogramma in response to bacteria from the surface of coralline algae","volume":"149","author":"Huggett","year":"2006","journal-title":"Oecologia"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3354\/meps09192","article-title":"Larvae of Pocillopora damicornis (Anthozoa) settle and metamorphose in response to surface-biofilm bacteria","volume":"433","author":"Tran","year":"2011","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1038\/s41598-020-59506-1","article-title":"Monospecifc bioflms of Pseudoalteromonas promote larval settlement and metamorphosis of Mytilus coruscus","volume":"10","author":"Peng","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/j.dci.2007.01.005","article-title":"Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis","volume":"31","author":"Bacque","year":"2007","journal-title":"Dev. Comp. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Puill-Stephan, E., Seneca, F.O., Miller, D.J., van Oppen, M.J.H., and Willis, B.L. (2012). Expression of putative immune response genes during early ontogeny in the coral Acropora millepora. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0039099"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0022-0981(85)90059-0","article-title":"Induction of settlement and metamorphosis of the pacific oyster, Crassostrea gigas (Thunberg), by L-DOPA and catecholamines","volume":"94","author":"Coon","year":"1985","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1080\/0892701021000060860","article-title":"Pharmacological induction of larval settlement and metamorphosis in the blue mussel Mytilus edulis L","volume":"19","author":"Dobretsov","year":"2003","journal-title":"Biofouling"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/S0044-8486(02)00567-7","article-title":"Larval settlement of the silver- or goldlip pearl oyster Pinctada maxima (Jameson) in response to natural biofilms and chemical cues","volume":"220","author":"Zhao","year":"2003","journal-title":"Aquaculture"},{"key":"ref_46","first-page":"2505","article-title":"siRNA transfection in larvae of the barnacle Amphibalanus amphitrite","volume":"218","author":"Zhang","year":"2015","journal-title":"J. Exp. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"13526","DOI":"10.1038\/s41598-020-70322-5","article-title":"Neurotrophic factors and target-specifc retrograde signaling interactions define the specificity of classical and neuropeptide cotransmitter release at identified Lymnaea synapses","volume":"10","author":"Getz","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1016\/j.steroids.2012.08.009","article-title":"Do mollusks use vertebrate sex steroids as reproductive hormones? Part I: Critical appraisal of the evidence for the presence, biosynthesis and uptake of steroids","volume":"77","author":"Scott","year":"2012","journal-title":"Steroids"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.steroids.2012.11.006","article-title":"Do mollusks use vertebrate sex steroids as reproductive hormones? II. Critical review of the evidence that steroids have biological effects","volume":"78","author":"Scott","year":"2013","journal-title":"Steroids"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"R726","DOI":"10.1016\/j.cub.2011.07.030","article-title":"The origins and evolution of vertebrate metamorphosis","volume":"21","author":"Laudet","year":"2011","journal-title":"Curr. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S1532-0456(01)00271-X","article-title":"Thyroid hormones in growth and development of fish","volume":"130","author":"Power","year":"2001","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/j.cub.2008.04.078","article-title":"Amphioxus postembryonic development reveals the homology of chordate metamorphosis","volume":"18","author":"Paris","year":"2008","journal-title":"Curr. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1002\/jez.1084","article-title":"Presence of thyroid hormones in ascidian larvae and their involvement in metamorphosis","volume":"290","author":"Patricolo","year":"2001","journal-title":"J. Exp. Zool."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1046\/j.1444-2906.2001.00351.x","article-title":"Induction of abalone larval metamorphosis by thyroid hormones","volume":"67","author":"Fukazawa","year":"2001","journal-title":"Fish. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1007\/s00343-019-8207-9","article-title":"The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas","volume":"37","author":"Huang","year":"2019","journal-title":"J. Oceanol. Limnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s00343-019-7359-y","article-title":"The roles of thyroid hormone receptor and T3 in metamorphosis of Haliotis diversicolor","volume":"37","author":"Wang","year":"2019","journal-title":"J. Oceanol. Limnol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.fsi.2012.05.011","article-title":"An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation","volume":"33","author":"Wu","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.mce.2017.03.021","article-title":"Evolution of ligands, receptors and metabolizing enzymes of thyroid signaling","volume":"459","author":"Holzer","year":"2017","journal-title":"Mol. Cell Endocrinol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S1084-9521(02)00179-9","article-title":"An overview of the Notch signalling pathway","volume":"14","author":"Baron","year":"2003","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1186\/1471-2148-9-249","article-title":"Origin and evolution of the Notch signalling pathway: An overview from eukaryotic genomes","volume":"9","author":"Gazave","year":"2009","journal-title":"BMC Evol. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1126\/science.284.5415.770","article-title":"Notch signaling: Cell fate control and signal integration in development","volume":"284","author":"Rand","year":"1999","journal-title":"Science"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"160242","DOI":"10.1098\/rsob.160242","article-title":"The Notch pathway in the annelid Platynereis: Insights into chaetogenesis and neurogenesis processes","volume":"7","author":"Gazave","year":"2017","journal-title":"Open Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/j.1525-142X.2005.05062.x","article-title":"Characterization of Notch-class gene expression in segmentation stem cells and segment founder cells in Helobdella robusta (Lophotrochozoa; Annelida; Clitellata; Hirudinida; Glossiphoniidae)","volume":"7","author":"Rivera","year":"2005","journal-title":"Evol. Dev."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"6534","DOI":"10.1038\/srep06534","article-title":"Transcriptome analysis elucidates key developmental components of bryozoan lophophore development","volume":"4","author":"Wong","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Feng, D.D., Li, Q., Yu, H., Zhao, X.L., and Kong, L.F. (2015). Comparative transcriptome analysis of the Pacific oyster Crassostrea gigas characterized by shell colors: Identification of genetic bases potentially involved in pigmentation. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0145257"},{"key":"ref_66","first-page":"100598","article-title":"Transcriptome analysis of shell color-related genes in the clam Meretrix meretrix","volume":"31","author":"Yue","year":"2015","journal-title":"Mar. Biotechnol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0092-8674(00)81873-5","article-title":"Programmed Cell Death in Animal Development","volume":"88","author":"Jacobson","year":"1997","journal-title":"Cell"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1007\/s11033-014-3833-y","article-title":"Molecular cloning of two molluscan caspases and gene functional analysis during Crassostrea angulata (Fujian oyster) larval metamorphosis","volume":"42","author":"Yang","year":"2014","journal-title":"Mol. Biol. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1002\/jez.b.21344","article-title":"Involvement of a novel p38 mitogen-activated protein kinase in larval metamorphosis of the polychaete Hydroides elegans (Haswell)","volume":"314","author":"Wang","year":"2010","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.1242\/dev.129.13.3105","article-title":"Tail regression in Ciona intestinalis (Prochordate) involves a Caspase-dependent apoptosis event associated with ERK activation","volume":"129","author":"Chambon","year":"2002","journal-title":"Development"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"4002","DOI":"10.1038\/s41598-018-22397-4","article-title":"Sea urchin histamine receptor 1 regulates programmed cell death in larval Strongylocentrotus purpuratus","volume":"8","author":"Lutek","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1007\/s10126-007-9053-x","article-title":"Understanding marine mussel adhesion","volume":"9","author":"Silverman","year":"2007","journal-title":"Mar. Biotechnol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"3782","DOI":"10.1073\/pnas.0607852104","article-title":"Adhesion mechanisms of the mussel foot proteins mfp-1 and mfp-3","volume":"104","author":"Lin","year":"2007","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nclimate1846","article-title":"Mussel byssus attachment weakened by ocean acidification","volume":"3","author":"George","year":"2013","journal-title":"Nature Clim. Change"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.msec.2015.04.032","article-title":"Functional Surface of the golden mussel\u2019s foot: Morphology, structures and the role of cilia on underwater adhesion","volume":"54","author":"Andrade","year":"2015","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Foulon, V., Boudry, P., Artigaud, S., Gu\u00e9rard, F., and Hellio, C. (2019). In silico analysis of Pacific oyster (Crassostrea gigas) transcriptome over developmental stages reveals candidate genes for larval settlement. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20010197"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"15018","DOI":"10.1038\/srep15018","article-title":"Extensible byssus of Pinctada fucata: Ca2+-stabilized nanocavities and a thrombospondin-1 protein","volume":"5","author":"Liu","year":"2015","journal-title":"Sci. Rep."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/14\/2\/287\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:13:09Z","timestamp":1760119989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/14\/2\/287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,21]]},"references-count":77,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["genes14020287"],"URL":"https:\/\/doi.org\/10.3390\/genes14020287","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,21]]}}}