{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:30:26Z","timestamp":1769128226249,"version":"3.49.0"},"reference-count":66,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T00:00:00Z","timestamp":1722643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Funds","award":["UIDB\/05183\/2020"],"award-info":[{"award-number":["UIDB\/05183\/2020"]}]},{"name":"National Funds","award":["LA\/P\/0121\/2020"],"award-info":[{"award-number":["LA\/P\/0121\/2020"]}]},{"name":"National Funds","award":["CEECINST\/00052\/2021"],"award-info":[{"award-number":["CEECINST\/00052\/2021"]}]},{"name":"National Funds","award":["RYC2022-036888-I"],"award-info":[{"award-number":["RYC2022-036888-I"]}]},{"name":"MCIU\/AEI\/10.13039\/501100011033","award":["UIDB\/05183\/2020"],"award-info":[{"award-number":["UIDB\/05183\/2020"]}]},{"name":"MCIU\/AEI\/10.13039\/501100011033","award":["LA\/P\/0121\/2020"],"award-info":[{"award-number":["LA\/P\/0121\/2020"]}]},{"name":"MCIU\/AEI\/10.13039\/501100011033","award":["CEECINST\/00052\/2021"],"award-info":[{"award-number":["CEECINST\/00052\/2021"]}]},{"name":"MCIU\/AEI\/10.13039\/501100011033","award":["RYC2022-036888-I"],"award-info":[{"award-number":["RYC2022-036888-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horticulturae"],"abstract":"<jats:p>The objective of this study was to analyze the chemical profile of extracts from different fruit parts of the perennial arborescent cactus Opuntia leucotricha by applying ultra-high-performance liquid chromatography\u2013high-resolution mass spectrometry (UHPLC-HRMS) and to evaluate their biological properties (antioxidant and enzyme inhibitory capacities). Extracts were obtained from the fruit pulp (without or with seeds), seeds, and peel by ultrasound-assisted extraction (UAE) using the conventional solvent methanol 50% and two Natural Deep Eutectic Solvents (NADESs) (glycerol:urea, 1:1 and citric acid:sorbitol, 1:2). A total of 33 compounds were identified, including phenolics, fatty acids, and others. Phenolic acids were the most abundant class of phenolics identified in all fruit parts, with the highest concentration observed in the methanol extracts from peel (593.02 \u00b5g\/gDW), followed by seed-containing pulp (69.03 \u00b5g\/gDW), pulp (57.83 \u00b5g\/gDW), and seeds (39.97 \u00b5g\/gDW). The second most effective extractant was NADES 1 (glycerol:urea, 1:1), which was also successful in extracting compounds with antioxidant capacity. Overall, the extracts demonstrated considerable enzyme inhibitory activities, with the greatest effects observed against \u03b1-amylase and \u03b1-glucosidase. The results indicate that O. leucotricha fruits could be a promising source of bioactive compounds, and NADES a viable alternative to organic solvents for their industrial exploitation.<\/jats:p>","DOI":"10.3390\/horticulturae10080824","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T15:45:22Z","timestamp":1722872722000},"page":"824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Phytochemical Characterization and Bioactivity of Extracts from Different Fruit Parts of Opuntia leucotricha DC.: A Comparison between a Conventional Organic Solvent and Green Natural Deep Eutectic Solvents"],"prefix":"10.3390","volume":"10","author":[{"given":"Ouafaa","family":"Hamdoun","sequence":"first","affiliation":[{"name":"Plant Biotechnology Team, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan 93000, Morocco"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3038-4434","authenticated-orcid":false,"given":"Sandra","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9632-8876","authenticated-orcid":false,"given":"In\u00eas","family":"Mansinhos","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4204-7387","authenticated-orcid":false,"given":"Raquel","family":"Rodr\u00edguez-Solana","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Department of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Rancho de la Merced Center, Carretera Ca\u00f1ada de la Loba (CA-3102) Km 3.1., SN, 11471 Jerez de la Frontera, C\u00e1diz, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3162-0432","authenticated-orcid":false,"given":"Gema","family":"Pereira-Caro","sequence":"additional","affiliation":[{"name":"Department of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Alameda del Obispo Center, Avenida Menendez-Pidal, SN, 14004 C\u00f3rdoba, Spain"},{"name":"Foods for Health Group, Instituto Maim\u00f3nides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC), 14004 C\u00f3rdoba, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9372-1346","authenticated-orcid":false,"given":"Jos\u00e9 Manuel","family":"Moreno-Rojas","sequence":"additional","affiliation":[{"name":"Department of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Alameda del Obispo Center, Avenida Menendez-Pidal, SN, 14004 C\u00f3rdoba, Spain"},{"name":"Foods for Health Group, Instituto Maim\u00f3nides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC), 14004 C\u00f3rdoba, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6656-3688","authenticated-orcid":false,"given":"Mohammed","family":"L\u2019bachir El Kbiach","sequence":"additional","affiliation":[{"name":"Plant Biotechnology Team, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan 93000, Morocco"}]},{"given":"Brahim","family":"El Bouzdoudi","sequence":"additional","affiliation":[{"name":"Plant Biotechnology Team, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan 93000, Morocco"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7204-7428","authenticated-orcid":false,"given":"Anabela","family":"Romano","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Guerrero-Beltr\u00e1n, J., and Ochoa-Velasco, C.E. 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