{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:27:22Z","timestamp":1772252842656,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T00:00:00Z","timestamp":1644624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/05183\/2020"],"award-info":[{"award-number":["UIDB\/05183\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horticulturae"],"abstract":"<jats:p>The present research intents to study skin fatty acids and physiological disorders developed during cold storage in \u2018Golden Delicious\u2019 apples treated with 1-MCP and calcium. Harvested fruits were treated with calcium chloride (Ca), 1-MCP (MCP), Ca + MCP or no treatment (control) and then subjected to cold storage at 0.5 \u00b0C for 6 months. Fatty acids\u2019 composition, malondialdehyde (MDA) and the physiological disorders bitter pit (BP), superficial scald and diffuse skin browning (DSB) were measured at harvest and after storage plus 7 days of shelf-life at room temperature \u224822 \u00b0C. Palmitic acid decreased and linoleic acid increased over time, while oleic and stearic acids had few changes. Generally, unsaturated\/saturated fatty acids and MDA increased over the storage period. Treatment with Ca showed that, at the end of the experiment, the lowest MDA values and the highest unsaturated\/saturated fatty acids ratio were mainly due to higher linoleic and lower palmitic acids concentrations, which are coincident with less severe BP. There was no clear correlation between the measured fatty acids (palmitic, linoleic, oleic and stearic), unsaturated-to-saturated fatty acids ratio or MDA with chilling skin physiological disorders. Further research is needed to clarify the changes in membrane properties and the effect of some treatments in response to chilling injury during storage.<\/jats:p>","DOI":"10.3390\/horticulturae8020162","type":"journal-article","created":{"date-parts":[[2022,2,13]],"date-time":"2022-02-13T21:08:43Z","timestamp":1644786523000},"page":"162","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Membrane Fatty Acids and Physiological Disorders in Cold-Stored \u2018Golden Delicious\u2019 Apples Treated with 1-MCP and Calcium Chloride"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8913-6136","authenticated-orcid":false,"given":"Maria Dulce","family":"Antunes","sequence":"first","affiliation":[{"name":"MED, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Edf. 8, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Ana Clara","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio de Patos de Minas\u2014UNIPAM, Rua Major Gote, 808-Bairro Cai\u00e7aras, Patos de Minas 38700-207, MG, Brazil"}]},{"given":"Cust\u00f3dia","family":"Gago","sequence":"additional","affiliation":[{"name":"MED, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Edf. 8, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Adriana","family":"Guerreiro","sequence":"additional","affiliation":[{"name":"CEOT, Faculdade de Ci\u00eancias e Tecnologia, Edf. 8, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Jorge","family":"Panagopoulos","sequence":"additional","affiliation":[{"name":"MED, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Edf. 8, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"C-MAST, Department of Aerospace Sciences, Faculty of Engineering, Universidade da Beira Interior, 62001-001 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0252-695X","authenticated-orcid":false,"given":"Eduardo","family":"Vilas Boas","sequence":"additional","affiliation":[{"name":"Department of Food Science, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2507-4228","authenticated-orcid":false,"given":"Maria Gra\u00e7a","family":"Miguel","sequence":"additional","affiliation":[{"name":"MED, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Edf. 8, Campus de Gambelas, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1590\/S0103-84782010000600032","article-title":"Pre-harvest applications of calcium in post-harvest quality of \u2018Fuji\u2019 apples","volume":"40","author":"Brackmann","year":"2010","journal-title":"Cienc. Rural"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.postharvbio.2015.07.018","article-title":"Effect of harvest date and 1-MCP (SmartFreshTM) treatment on \u2018Golden Delicious\u2019 apple cold storage physiological disorders","volume":"110","author":"Gago","year":"2015","journal-title":"Postharvest Biol. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2786","DOI":"10.1021\/jf802564z","article-title":"Superficial scald and bitter pit development in cold-stored transgenic apples suppressed for ethylene biosynthesis","volume":"57","author":"Pesis","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.scienta.2016.09.017","article-title":"Effect of calcium chloride and 1-MCP (SmartfreshTM) postharvest treatment on \u2018Golden Delicious\u2019 apple cold storage physiological disorders","volume":"211","author":"Gago","year":"2016","journal-title":"Sci. Hortic."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/9781118060841.ch8","article-title":"Bitter-pit in apple fruit","volume":"11","author":"Ferguson","year":"1989","journal-title":"Hortic. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.postharvbio.2010.02.006","article-title":"Cellular approach to understand bitter pit development in apple fruit","volume":"57","author":"Freitas","year":"2010","journal-title":"Postharvest Biol. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1002\/9781118060834.ch4","article-title":"Calcium and fruit softening: Physiology and biochemistry","volume":"10","author":"Poovaiah","year":"1988","journal-title":"Hortic. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.postharvbio.2011.11.001","article-title":"Superficial scald, its etiology and control","volume":"65","author":"Lurie","year":"2012","journal-title":"Postharvest Biol. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gago, C., Ant\u00e3o, R., Dores, C., Guerreiro, A., Miguel, M.G., Faleiro, M.L., Figueiredo, A.C., and Antunes, M.D. (2020). The effect of nanocoatings enriched with essential oils on \u2018Rocha\u2019 pear long storage. Foods, 9.","DOI":"10.3390\/foods9020240"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0925-5214(95)00042-9","article-title":"Membrane effects in postharvest physiology","volume":"7","author":"Marangoni","year":"1996","journal-title":"Postharvest Biol. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1080\/14620316.2000.11511231","article-title":"Changes in ATP, ADP and pyridine nucleotide levels related to the incidence of physiological disorders in \u2018Conference\u2019 pears and \u2018Jonagold\u2019 apples during controlled atmosphere storage","volume":"75","author":"Saquet","year":"2000","journal-title":"J. Hortic. Sci. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.postharvbio.2007.10.017","article-title":"Chilling-induced oxidative stress and antioxidant responses in mume (Prunus mume) fruit during low temperature storage","volume":"49","author":"Imahori","year":"2008","journal-title":"Postharvest Biol. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.postharvbio.2015.05.012","article-title":"1-Methylcyclopropene alleviates chilling injury by regulating energy metabolism and fatty acid content in \u2018Nanguo\u2019 pears","volume":"109","author":"Cheng","year":"2015","journal-title":"Postharvest Biol. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1016\/j.foodchem.2009.01.082","article-title":"Methyl jasmonate reduces chilling injury and enhances antioxidant enzyme activity in postharvest loquat fruit","volume":"115","author":"Cao","year":"2009","journal-title":"Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1023\/B:GROW.0000040112.19837.5f","article-title":"Effects of chilling temperatures on ethylene binding by banana fruit","volume":"43","author":"Jiang","year":"2004","journal-title":"Plant Growth Regul."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.foodchem.2013.06.132","article-title":"Pre-storage application of oxalic acid alleviates chilling injury in mango fruit by modulating proline metabolism and energy status under chilling stress","volume":"142","author":"Li","year":"2014","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1104\/pp.123.4.1449","article-title":"Ethylene perception by the ERS1 protein in Arabidopsis","volume":"123","author":"Hall","year":"2000","journal-title":"Plant Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.jfoodeng.2010.08.028","article-title":"Modeling the diffusion\u2013adsorption kinetics of 1-methylcyclopropene (1-MCP) in apple fruit and non-target materials in storage rooms","volume":"102","author":"Ambaw","year":"2011","journal-title":"J. Food Eng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/14620316.2000.11511236","article-title":"Inhibitory effect of 1-MCP on ripening and superficial scald development in \u2018McIntosh\u2019 and \u2018Delicious\u2019 apples","volume":"75","author":"Rupasinghe","year":"2000","journal-title":"J. Hort. Sci. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"63","DOI":"10.17660\/ActaHortic.2010.857.5","article-title":"1-Methylcyclopropene (1-MCP) affects physiological disorders in \u2018Granny Smith\u2019 apples depending on maturity stage","volume":"857","author":"Calvo","year":"2010","journal-title":"Acta Hort."},{"key":"ref_21","first-page":"119","article-title":"Biochemical changes in 1-MCP treated skin tissue during cold storage and their relationship with physiological disorders","volume":"796","author":"Ubach","year":"2008","journal-title":"Acta Hort."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1002\/jsfa.4094","article-title":"\u2018Diffuse skin browning\u2019 in 1-MCP-treated apples: Etiology and systems of control","volume":"90","author":"Vilaplana","year":"2010","journal-title":"J. Sci. Food Agric."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"507","DOI":"10.17660\/ActaHortic.2010.877.65","article-title":"The complexity of preventing diffuse skin browning (DSB) on 1-MCP (Smartfresh) -treated \u2018GoldenDelicious\u2019 apples","volume":"877","author":"Gamrasni","year":"2010","journal-title":"Acta Hort."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7439","DOI":"10.1021\/jf8011322","article-title":"Development of a rapid method for the sequential extraction and subsequent quantification of fatty acids and sugars from avocado mesocarp tissue","volume":"56","author":"Meyer","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1007\/s004250050524","article-title":"Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds","volume":"207","author":"Hodges","year":"1999","journal-title":"Planta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.postharvbio.2011.11.003","article-title":"Antioxidant metabolism of 1-methylcyclopropene (1-MCP) treated \u2018Empire\u2019 apples during controlled atmosphere storage","volume":"65","author":"Lee","year":"2012","journal-title":"Postharvest Biol. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.foodchem.2006.07.030","article-title":"Chemical compositional characterization of some apple cultivars","volume":"103","author":"Wu","year":"2007","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.foodchem.2019.05.117","article-title":"Insights into the metabolism of membrane lipid fatty acids associated with chilling injury in post-harvest bell peppers","volume":"295","author":"Ge","year":"2019","journal-title":"Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/j.foodchem.2008.02.089","article-title":"Changes in fatty acid composition and electrolyte leakage of \u2018Hayward\u2019 kiwifruit during storage at different temperatures","volume":"110","author":"Antunes","year":"2008","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.foodchem.2017.10.008","article-title":"Melatonin treatment reduces chilling injury in peach fruit through its regulation of membrane fatty acid contents and phenolic metabolism","volume":"245","author":"Gao","year":"2018","journal-title":"Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.postharvbio.2014.07.010","article-title":"Chilling injury in pineapple fruit: Fatty acid composition and antioxidant metabolism","volume":"99","author":"Nukuntornprakit","year":"2015","journal-title":"Postharvest Biol. Technol."},{"key":"ref_32","unstructured":"Smirnoff, N. (1995). Antioxidant systems and plant response to the environment. Environment and Plant Metabolism: Flexibility and Acclimation, Bios Scientific Publishers."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1007\/s11947-017-1935-y","article-title":"Control of postharvest quality in blueberry fruit by combined 1-methylcyclopropene (1-MCP) and UV-C irradiation","volume":"10","author":"Xu","year":"2017","journal-title":"Food Bioproc. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"205","DOI":"10.21273\/HORTSCI.30.4.205","article-title":"Peroxidative activity of apple peel in relation to development of poststorage disorders","volume":"30","author":"Du","year":"1995","journal-title":"HortScience"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/jfpp.14925","article-title":"Combination of carnauba wax-based coating and 1-methylcyclopropene (1-MCP) maintains better \u201cFuji\u201d apple qualities during storage at low temperature","volume":"44","author":"Chen","year":"2020","journal-title":"J. Food Process. Preserv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"232","DOI":"10.21273\/JASHS.130.2.232","article-title":"Expression of \u03b1-farnesene synthase AFS1 and 3-hydroxy-3-methylglutaryl-coenzymea reductase HMG2 and HMG3 in relation to \u03b1-farnesene and conjugated trienolsin \u2018Granny Smith\u2019 apples heat or 1-MCP treated to prevent superficial scald","volume":"130","author":"Lurie","year":"2005","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1051\/fruits\/2010013","article-title":"Fruit quality of Granny Smith apples picked at different harvest times and treated with 1-MCP","volume":"65","author":"Magazin","year":"2010","journal-title":"Fruits"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.21273\/HORTSCI.40.5.1534","article-title":"Response of four apple cultivars to 1-methylcyclopropene treatment and controlled atmosphere storage","volume":"40","author":"Bai","year":"2005","journal-title":"HortScience"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.21273\/HORTSCI.40.7.2096","article-title":"Effects of delays between harvest and 1-methylcyclopropene treatment, and temperature during treatment, on ripening of air-stored and controlled atmosphere-stored Apples","volume":"40","author":"Watkins","year":"2005","journal-title":"HortScience"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1590\/S0100-204X2011000700003","article-title":"Physiological, physical, chemical and mineral attributes associated with the occurrence of \u201cbitter pit\u201d in apples","volume":"46","author":"Miqueloto","year":"2011","journal-title":"Pesqui. Agropecu. Bras."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hou, Y., Li, Z., Zheng, Y., and Jin, P. (2021). Effects of CaCl2 treatment alleviates chilling injury of loquat fruit (Eribotrya japonica) by modulating ROS homeostasis. Foods, 10.","DOI":"10.3390\/foods10071662"}],"container-title":["Horticulturae"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2311-7524\/8\/2\/162\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:18:22Z","timestamp":1760134702000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2311-7524\/8\/2\/162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,12]]},"references-count":41,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["horticulturae8020162"],"URL":"https:\/\/doi.org\/10.3390\/horticulturae8020162","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints202201.0191.v1","asserted-by":"object"}]},"ISSN":["2311-7524"],"issn-type":[{"value":"2311-7524","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,12]]}}}