{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T09:59:04Z","timestamp":1772531944623,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T00:00:00Z","timestamp":1470096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U\u2219L\u22121) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.<\/jats:p>","DOI":"10.3390\/ijerph13080778","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T03:47:39Z","timestamp":1470196059000},"page":"778","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4069-509X","authenticated-orcid":false,"given":"Cristiane","family":"Ottoni","sequence":"first","affiliation":[{"name":"Biosciences Institute, S\u00e3o Paulo State University\u2014UNESP, Coastal Campus, S\u00e3o Vicente, S\u00e3o Paulo 11330-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8767-9487","authenticated-orcid":false,"given":"Marta","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Biology Department, Edge Hill University, St. Helens Road, Lancashire, Ormskirk L39 4QP, UK"}]},{"given":"Sara","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Richmond Pharmacology Ltd., St. George\u2019s University London, Cranmer Terrace, London SW17 0RE, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4681-0941","authenticated-orcid":false,"given":"Cledir","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences and Natural Resources, Centro de Excelencia en Investigaci\u00f3n Biotecnol\u00f3gica Aplicada al Medio Ambiente (CIBAMA), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811-230, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2185-0613","authenticated-orcid":false,"given":"Nelson","family":"Lima","sequence":"additional","affiliation":[{"name":"CEB-Centre of Biological Engineering, Micoteca da Universidade do Minho, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2016,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1002\/jobm.201100341","article-title":"Production of laccase from Trametes trogii TEM H2: A newly isolated white-rot fungus by air sampling","volume":"52","author":"Kocyigit","year":"2012","journal-title":"J. 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