{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T08:42:23Z","timestamp":1770540143154,"version":"3.49.0"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001655","name":"Deutscher Akademischer Austauschdienst","doi-asserted-by":"publisher","award":["57397247"],"award-info":[{"award-number":["57397247"]}],"id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/00511\/2017 and DL57\/2016\/CP1479\/CT0024"],"award-info":[{"award-number":["CEECIND\/00511\/2017 and DL57\/2016\/CP1479\/CT0024"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The increasing number of pharmaceuticals in the environment and their difficult biodegradation, can lead to bioaccumulation in different trophic compartments. Their bioaccumulation can have negative consequences, especially in the generation of bacterial resistance by antibiotics, but also in the impairment of plant and animal metabolism. The Tejo estuary in Portugal is the habitat for many plant and animal species, which are also prone to this type of contamination. Therefore, in the present study different classes of emerging pollutants (EPs) were surveyed in water samples in the Tejo estuary, including antibiotics, anticonvulsants, antidepressants, lipid-lowering drugs, anti-inflammatory drugs, beta-blockers and analgesics. According to the results, only four compounds were detected in water samples collected at the three selected salt marshes, including carbamazepine, fluoxetine hydrochloride, venlafaxine hydrochloride and acetaminophen. Having the detected substances as a basis, a subsequent study was performed aiming to investigate the uptake and biodegradation capacity of halophytes, using Tripolium pannonicum as a model plant cultivated under controlled conditions with different concentrations of the found EPs. This experimental approach showed that T. pannonicum was able to uptake and degrade xenobiotics. Moreover, the application of sulfamethazine, as a model antibiotic, showed also that this species can uptake and degrade this compound, although the degradation rate and process proved to be compound-specific. This was also confirmed using crude plant extracts spiked with the different EPs. Thus this species is a potential candidate for the remediation of marine water and sediments contaminated with environmentally-significant EPs.<\/jats:p>","DOI":"10.3390\/ijerph18030943","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T11:13:53Z","timestamp":1611314033000},"page":"943","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Screening of Emerging Pollutants (EPs) in Estuarine Water and Phytoremediation Capacity of Tripolium pannonicum under Controlled Conditions"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0015-5742","authenticated-orcid":false,"given":"Ariel E.","family":"Turcios","sequence":"first","affiliation":[{"name":"Institute of Botany, Leibniz University Hannover, Herrenh\u00e4userstr. 2, D-30419 Hannover, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2510-5752","authenticated-orcid":false,"given":"Marie","family":"Hielscher","sequence":"additional","affiliation":[{"name":"Institute of Botany, Leibniz University Hannover, Herrenh\u00e4userstr. 2, D-30419 Hannover, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1914-7435","authenticated-orcid":false,"given":"Bernardo","family":"Duarte","sequence":"additional","affiliation":[{"name":"MARE-Marine and Environmental Sciences Centre, Faculdade de Ci\u00eancias da Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal"},{"name":"Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias da Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6374-1194","authenticated-orcid":false,"given":"Vanessa F.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"MARE-Marine and Environmental Sciences Centre, Faculdade de Ci\u00eancias da Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal"},{"name":"Departamento de Biologia Animal, Faculdade de Ci\u00eancias da Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal"}]},{"given":"Isabel","family":"Ca\u00e7ador","sequence":"additional","affiliation":[{"name":"MARE-Marine and Environmental Sciences Centre, Faculdade de Ci\u00eancias da Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal"},{"name":"Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias da Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0942-4072","authenticated-orcid":false,"given":"Jutta","family":"Papenbrock","sequence":"additional","affiliation":[{"name":"Institute of Botany, Leibniz University Hannover, Herrenh\u00e4userstr. 2, D-30419 Hannover, Germany"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.nbt.2014.01.001","article-title":"Emerging Pollutants in the Environment: Present and Future Challenges in Biomonitoring, Ecological Risks and Bioremediation","volume":"32","author":"Gavrilescu","year":"2015","journal-title":"New Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s10661-015-4374-0","article-title":"Occurrence and Analysis of Endocrine-Disrupting Compounds in a Water Supply System","volume":"187","author":"Carvalho","year":"2015","journal-title":"Environ. Monit. Assess"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.marpolbul.2018.08.036","article-title":"Screening of Human and Veterinary Pharmaceuticals in Estuarine Waters: A Baseline Assessment for the Tejo Estuary","volume":"135","author":"Pais","year":"2018","journal-title":"Mar. Pollut. Bull."},{"key":"ref_4","unstructured":"(2020, December 14). Chemicals Production and Consumption Statistics\u2014Statistics Explained. Available online: https:\/\/ec.europa.eu\/eurostat\/statistics-explained\/index.php\/Chemicals_production_and_consumption_statistics."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105110","DOI":"10.1016\/j.marenvres.2020.105110","article-title":"Engineered Metal Nanoparticles in the Marine Environment: A Review of the Effects on Marine Fauna","volume":"161","author":"Roma","year":"2020","journal-title":"Mar. Environ. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105569","DOI":"10.1016\/j.aquatox.2020.105569","article-title":"Effects of Antidepressants in the Reproduction of Aquatic Organisms: A Meta-Analysis","volume":"227","author":"Lopes","year":"2020","journal-title":"Aquat. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"136564","DOI":"10.1016\/j.scitotenv.2020.136564","article-title":"Depressed, Hypertense and Sore: Long-Term Effects of Fluoxetine, Propranolol and Diclofenac Exposure in a Top Predator Fish","volume":"712","author":"Duarte","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Cruz de Carvalho, R., Feij\u00e3o, E., Matos, A.R., Cabrita, M.T., Novais, S.C., Lemos, M.F.L., Ca\u00e7ador, I., Marques, J.C., Reis-Santos, P., and Fonseca, V.F. (2020). Glyphosate-Based Herbicide Toxicophenomics in Marine Diatoms: Impacts on Primary Production and Physiological Fitness. Appl. Sci., 10.","DOI":"10.3390\/app10217391"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.3389\/fmicb.2020.01803","article-title":"Fluoxetine Arrests Growth of the Model Diatom Phaeodactylum Tricornutum by Increasing Oxidative Stress and Altering Energetic and Lipid Metabolism","volume":"11","author":"Duarte","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"105109","DOI":"10.1016\/j.marenvres.2020.105109","article-title":"Comfortably Numb: Ecotoxicity of the Non-Steroidal Anti-Inflammatory Drug Ibuprofen on Phaeodactylum Tricornutum","volume":"161","author":"Silva","year":"2020","journal-title":"Mar. Environ. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1016\/j.scitotenv.2018.09.354","article-title":"Ecotoxicity of the Lipid-Lowering Drug Bezafibrate on the Bioenergetics and Lipid Metabolism of the Diatom Phaeodactylum Tricornutum","volume":"650","author":"Duarte","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1080\/10408440091159176","article-title":"Health Effects of Endocrine-Disrupting Chemicals on Wildlife, with Special Reference to the European Situation","volume":"30","author":"Vos","year":"2000","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_13","unstructured":"Bergmann, A., Fohrmann, R.U., and Weber, F. (2011). Zusammenstellung von Monitoringdaten Zu Umweltkonzentrationen von Arzneimitteln, Umweltbundesamt. UBA-Texte 66\/2011."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"E3463","DOI":"10.1073\/pnas.1717295115","article-title":"Global Increase and Geographic Convergence in Antibiotic Consumption between 2000 and 2015","volume":"115","author":"Klein","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"106437","DOI":"10.1016\/j.ecolind.2020.106437","article-title":"Roving Pharmacies: Modelling the Dispersion of Pharmaceutical Contamination in Estuaries","volume":"115","author":"Fonseca","year":"2020","journal-title":"Ecol. Indic."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Schr\u00f6der, P., and Collins, C.D. (2011). Organic Xenobiotics and Plants: From Mode of Action to Ecophysiology. Plant Ecophysiology, Springer.","DOI":"10.1007\/978-90-481-9852-8"},{"key":"ref_17","first-page":"75","article-title":"Biogeochemical Processes Governing Exposure and Uptake of Organic Pollutant Compounds in Aquatic Organisms","volume":"90","author":"Farrington","year":"1991","journal-title":"Environ. Health Perspect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.biortech.2016.08.047","article-title":"Uptake and Biodegradation of the Antimicrobial Sulfadimidine by the Species Tripolium Pannonicum Acting as Biofilter and Its Further Biodegradation by Anaerobic Digestion and Concomitant Biogas Production","volume":"219","author":"Turcios","year":"2016","journal-title":"Bioresour. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1080\/15320383.2019.1592108","article-title":"A Review on Heavy Metals Contamination in Soil: Effects, Sources, and Remediation Techniques","volume":"28","author":"Li","year":"2019","journal-title":"Soil Sediment Contam. Int. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"836","DOI":"10.3390\/su6020836","article-title":"Sustainable Treatment of Aquaculture Effluents\u2014What Can We Learn from the Past for the Future?","volume":"6","author":"Turcios","year":"2014","journal-title":"Sustainability"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"106816","DOI":"10.1016\/j.ecolind.2020.106816","article-title":"Halophyte Bio-Optical Phenotyping: A Multivariate Photochemical Pressure Index (Multi-PPI) to Classify Salt Marsh Anthropogenic Pressures Levels","volume":"119","author":"Kletschkus","year":"2020","journal-title":"Ecol. Indic."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.ecolind.2011.09.007","article-title":"Macroinvertebrates and Fishes as Biomonitors of Heavy Metal Concentration in the Seixal Bay (Tagus Estuary): Which Species Perform Better?","volume":"19","author":"Costa","year":"2012","journal-title":"Ecol. Indic."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1016\/j.envpol.2009.12.004","article-title":"Accumulation and Biological Cycling of Heavy Metal in Four Salt Marsh Species, from Tagus Estuary (Portugal)","volume":"158","author":"Duarte","year":"2010","journal-title":"Environ. Pollut."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ecolind.2012.09.021","article-title":"Development of an Angiosperm Quality Assessment Index (AQuA-Index) for Ecological Quality Evaluation of Portuguese Water Bodies\u2014A Multi-Metric Approach","volume":"25","author":"Neto","year":"2013","journal-title":"Ecol. Indic."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.envres.2014.09.041","article-title":"Environmental Impact of Pharmaceuticals from Portuguese Wastewaters: Geographical and Seasonal Occurrence, Removal and Risk Assessment","volume":"136","author":"Pereira","year":"2015","journal-title":"Environ. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1007\/s00216-010-4297-0","article-title":"Cleanup Strategies and Advantages in the Determination of Several Therapeutic Classes of Pharmaceuticals in Wastewater Samples by SPE\u2013LC\u2013MS\/MS","volume":"399","author":"Sousa","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.agwat.2014.11.001","article-title":"Optimization of Culturing Conditions and Selection of Species for the Use of Halophytes as Biofilter for Nutrient-Rich Saline Water","volume":"149","author":"Buhmann","year":"2015","journal-title":"Agric. Water Manag."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1080\/15226514.2019.1606782","article-title":"Enzymatic Degradation of the Antibiotic Sulfamethazine by Using Crude Extracts of Different Halophytic Plants","volume":"21","author":"Turcios","year":"2019","journal-title":"Int. J. Phytoremediat."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.biombioe.2016.01.013","article-title":"Effect of Salt and Sodium Concentration on the Anaerobic Methanisation of the Halophyte Tripolium Pannonicum","volume":"87","author":"Turcios","year":"2016","journal-title":"Biomass Bioenergy"},{"key":"ref_30","unstructured":"(2020, December 15). PubChem, Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1105\/tpc.112.096149","article-title":"Sulfamethazine Suppresses Epigenetic Silencing in Arabidopsis by Impairing Folate Synthesis","volume":"24","author":"Zhang","year":"2012","journal-title":"Plant Cell"},{"key":"ref_32","unstructured":"Sohel, A.J., Shutter, M.C., and Molla, M. (2020). Fluoxetine. StatPearls, StatPearls Publishing."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1104\/pp.109.138552","article-title":"Senescence-Induced Serotonin Biosynthesis and Its Role in Delaying Senescence in Rice Leaves","volume":"150","author":"Kang","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s10646-018-1919-3","article-title":"The Effects of Fluoxetine on Attachment and Righting Behaviours in Marine (Gibbula Unbilicalis) and Freshwater (Lymnea stagnalis) Gastropods","volume":"27","author":"Ford","year":"2018","journal-title":"Ecotoxicology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4327","DOI":"10.1007\/s11356-013-1693-y","article-title":"Biosolid-Borne Tetracyclines and Sulfonamides in Plants","volume":"20","author":"Mathews","year":"2013","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.jes.2018.02.022","article-title":"Biofiltration of the Antibacterial Drug Sulfamethazine by the Species Chenopodium Quinoa and Its Further Biodegradation through Anaerobic Digestion","volume":"75","author":"Turcios","year":"2019","journal-title":"J. Environ. Sci."},{"key":"ref_37","unstructured":"McCutcheon, S.C., and Schnoor, J.L. (2003). Uptake and Metabolism of Organic Compounds: Green-Liver Model. A Wiley-Interscience Series of Texts and Monographs, John Wiley & Sons, Inc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"164","DOI":"10.4491\/eer.2015.132","article-title":"Removal of Acetaminophen from Wastewater by Constructed Wetlands with Scirpus Validus","volume":"21","author":"Phong","year":"2016","journal-title":"Environ. Eng. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"124391","DOI":"10.1016\/j.chemosphere.2019.124391","article-title":"Acetaminophen Micropollutant: Historical and Current Occurrences, Toxicity, Removal Strategies and Transformation Pathways in Different Environments","volume":"236","author":"Le","year":"2019","journal-title":"Chemosphere"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1007\/s00253-012-4414-4","article-title":"Paracetamol in the Environment and Its Degradation by Microorganisms","volume":"96","author":"Wu","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6100","DOI":"10.1021\/acs.est.6b06485","article-title":"Extensive Transformation of the Pharmaceutical Carbamazepine Following Uptake into Intact Tomato Plants","volume":"51","author":"Riemenschneider","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6957","DOI":"10.1021\/acs.est.8b01682","article-title":"Pharmacokinetics in Plants: Carbamazepine and Its Interactions with Lamotrigine","volume":"52","author":"Goldstein","year":"2018","journal-title":"Environ. Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/s11356-008-0095-z","article-title":"Metabolism of Acetaminophen (Paracetamol) in Plants\u2014Two Independent Pathways Result in the Formation of a Glutathione and a Glucose Conjugate","volume":"16","author":"Huber","year":"2009","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ecoleng.2015.01.036","article-title":"Fate of Pharmaceuticals in a Subsurface Flow Constructed Wetland and Two Ponds","volume":"80","author":"Wick","year":"2015","journal-title":"Ecol. Eng."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1021\/es0494757","article-title":"Aqueous Photochemical Reaction Kinetics and Transformations of Fluoxetine","volume":"39","author":"Lam","year":"2005","journal-title":"Environ. Sci. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s11368-008-0024-2","article-title":"Low Biodegradability of Fluoxetine HCl, Diazepam and Their Human Metabolites in Sewage Sludge-Amended Soil","volume":"8","author":"Redshaw","year":"2008","journal-title":"J. Soils Sediments"}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/18\/3\/943\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:13:51Z","timestamp":1760159631000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/18\/3\/943"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,22]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["ijerph18030943"],"URL":"https:\/\/doi.org\/10.3390\/ijerph18030943","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,22]]}}}