{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:53:53Z","timestamp":1760151233545,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>In this study, chestnut shells (CNS), a recalcitrant and low-value agro-industrial waste obtained during the peeling of Castanea sativa fruits, were subjected to solid-state fermentation by six white-rot fungal strains (Irpex lacteus, Ganoderma resinaceum, Phlebia rufa, Bjerkandera adusta and two Trametes isolates). After being fermented, CNS was subjected to hydrolysis by a commercial enzymatic mix to evaluate the effect of fermentation in saccharification yield. After 48 h hydrolysis with 10 CMCase U mL\u22121 enzymatic mix, CNS fermented with both Trametes strains was recorded with higher saccharification yield (around 253 mg g\u22121 fermented CNS), representing 25% w\/w increase in reducing sugars as compared to non-fermented controls. To clarify the relationships and general mechanisms of fungal fermentation and its impacts on substrate saccharification, the effects of some independent or explanatory variables in the production of reducing sugars were estimated by general predictive saccharification models. The variables considered were lignocellulolytic activities in fungal fermentation, CNS hydrolysis time, and concentration of enzymatic hydrolysis mix. Multiple linear regression analysis revealed a very high significant effect (p &lt; 0.0001) of fungal laccase and xylanase activities in the saccharification models, thus proving the key potential of these enzymes in CNS solid-state fermentation.<\/jats:p>","DOI":"10.3390\/ijerph19052572","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T09:34:38Z","timestamp":1645608878000},"page":"2572","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Solid-State Fermentation of Chestnut Shells and Effect of Explanatory Variables in Predictive Saccharification Models"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9738-4658","authenticated-orcid":false,"given":"Paula A.","family":"Pinto","sequence":"first","affiliation":[{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environmental and Biological Sciences, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3475-4682","authenticated-orcid":false,"given":"Rui M. F.","family":"Bezerra","sequence":"additional","affiliation":[{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environmental and Biological Sciences, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Department of Biology and Environment, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"}]},{"given":"Irene","family":"Fraga","sequence":"additional","affiliation":[{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environmental and Biological Sciences, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Department of Biology and Environment, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5537-7353","authenticated-orcid":false,"given":"Carla","family":"Amaral","sequence":"additional","affiliation":[{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environmental and Biological Sciences, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Department of Biology and Environment, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7989-0779","authenticated-orcid":false,"given":"Ana","family":"Sampaio","sequence":"additional","affiliation":[{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environmental and Biological Sciences, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Department of Biology and Environment, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7048-7991","authenticated-orcid":false,"given":"Albino A.","family":"Dias","sequence":"additional","affiliation":[{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environmental and Biological Sciences, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Department of Biology and Environment, UTAD\u2014Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Li, Y., Chiu, Y.H., and Lin, T.Y. (2019). Research on New and Traditional Energy Sources in OECD Countries. Int. J. Environ. Res. Public Health, 16.","DOI":"10.3390\/ijerph16071122"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1080\/10942912.2019.1656233","article-title":"Soluble and insoluble-bound phenolics and antioxidant activity of various industrial plant wastes","volume":"22","author":"Gulsunoglu","year":"2019","journal-title":"Int. J. Food Prop."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Esposito, T., Celano, R., Pane, C., Piccinelli, A.L., Sansone, F., Picerno, P., Zaccardelli, M., Aquino, R.P., and Mencherini, T. (2019). Chestnut (Castanea sativa Miller.) burs extracts and functional compounds: UHPLC-UV-HRMS profiling, antioxidant activity, and inhibitory effects on phytopathogenic fungi. Molecules, 24.","DOI":"10.3390\/molecules24020302"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.tifs.2018.10.005","article-title":"Chestnuts and by-products as source of natural antioxidants in meat and meat products: A review","volume":"82","author":"Echegaray","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Fernandes, J.M.C., Fraga, I., Sousa, R.M.O., Rodrigues, M.A.M., Sampaio, A., Bezerra, R.M.F., and Dias, A.A. (2020). Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17165900"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Lee, K.H., Lee, S.K., Lee, J., Kim, S., Park, C., Kim, S.W., and Yoo, H.Y. (2021). Improvement of Enzymatic Glucose Conversion from Chestnut Shells through Optimization of KOH Pretreatment. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18073772"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.cej.2012.01.019","article-title":"Alkaline pre-treatment of waste chestnut shell from a food industry to enhance cadmium, copper, lead and zinc ions removal","volume":"184","author":"Mosquera","year":"2012","journal-title":"Chem. Eng. J."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Morana, A., Squillaci, G., Paix\u00e3o, S.M., Alves, L., la Cara, F., and Moura, P. (2017). Development of an energy biorefinery model for chestnut (Castanea sativa Mill.) shells. Energies, 10.","DOI":"10.3390\/en10101504"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.energy.2017.01.005","article-title":"Insight into progress in pre-treatment of lignocellulosic biomass","volume":"122","author":"Bhutto","year":"2017","journal-title":"Energy"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1007\/s12649-017-0177-6","article-title":"Choice of pretreatment technology for sustainable production of bioethanol from lignocellulosic biomass: Bottle necks and recommendations","volume":"10","author":"Kumar","year":"2019","journal-title":"Waste Biomass Valoriz."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3389\/fchem.2018.00141","article-title":"Lignocellulosic biomass transformations via greener oxidative pretreatment processes: Access to energy and value-added chemicals","volume":"6","author":"Den","year":"2018","journal-title":"Front. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1016\/j.biotechadv.2012.03.003","article-title":"Fungal pretreatment of lignocellulosic biomass","volume":"30","author":"Wan","year":"2012","journal-title":"Biotechnol. Adv."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.rser.2019.01.048","article-title":"Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production","volume":"105","author":"Zabed","year":"2019","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3389\/fenrg.2018.00141","article-title":"Recent trends in the pretreatment of lignocellulosic biomass for value-added products","volume":"6","author":"Baruah","year":"2018","journal-title":"Front. Energy Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Wagner, A.O., Lackner, N., Mutschlechner, M., Prem, E.M., Markt, R., and Illmer, P. (2018). Biological pretreatment strategies for second-generation lignocellulosic resources to enhance biogas production. Energies, 11.","DOI":"10.3390\/en11071797"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.biombioe.2008.04.016","article-title":"Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production","volume":"33","author":"Shi","year":"2009","journal-title":"Biomass Bioeng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.biortech.2012.02.068","article-title":"Influence of ligninolytic enzymes on straw saccharification during fungal pretreatment","volume":"111","author":"Pinto","year":"2012","journal-title":"Bioresour. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6045","DOI":"10.1016\/j.biortech.2010.02.110","article-title":"Enzymatic saccharification of biologically pre-treated wheat straw with white-rot fungi","volume":"101","author":"Dias","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1007\/s00284-014-0743-0","article-title":"Characterization of Lignocellulolytic Enzymes from White-Rot Fungi","volume":"70","author":"Manavalan","year":"2015","journal-title":"Curr. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1385\/ABAB:112:3:173","article-title":"Discrimination among eight modified Michaelis-Menten kinetics models of cellulose hydrolysis with a large range of substrate\/enzyme ratios: Inhibition by cellobiose","volume":"112","author":"Bezerra","year":"2004","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1021\/ac60147a030","article-title":"Use of dinitrosalicylic acid reagent for determination of reducing sugar","volume":"31","author":"Miller","year":"1959","journal-title":"Anal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.jfoodeng.2004.06.005","article-title":"Chestnut shell and barley bran as potential substrates for laccase production by Coriolopsis rigida under solid-state conditions","volume":"68","author":"Pazos","year":"2005","journal-title":"J. Food Eng."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4829","DOI":"10.1016\/j.biortech.2009.04.036","article-title":"Modification of wheat straw lignin by solid state fermentation with white-rot fungi","volume":"100","author":"Dinis","year":"2009","journal-title":"Bioresour. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.jbiosc.2009.04.023","article-title":"Lignocellulose degradation and enzyme production by Irpex lacteus CD2 during solid-state fermentation of corn stover","volume":"108","author":"Xu","year":"2009","journal-title":"J. Biosci. Bioeng."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s10532-021-09936-z","article-title":"Removal pattern of vinasse phenolics by Phlebia rufa, characterization of an induced laccase and inhibition kinetics modeling","volume":"32","author":"Fernandes","year":"2021","journal-title":"Biodegradation"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9248","DOI":"10.1016\/j.biortech.2010.07.042","article-title":"Production of lignocellulolytic enzymes and enhancement of in vitro digestibility during solid state fermentation of wheat straw by Phlebia floridensis","volume":"101","author":"Sharma","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1007\/s00449-013-1045-9","article-title":"Biodegradation of chestnut shell and lignin-modifying enzymes production by the white-rot fungi Dichomitus squalens, Phlebia radiata","volume":"37","author":"Dong","year":"2014","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/S1001-0742(08)60014-5","article-title":"Effects of culture conditions on ligninolytic enzymes and protease production by Phanerochaete chrysosporium in air","volume":"20","author":"Xiaoping","year":"2008","journal-title":"J. Environ. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0168-1656(90)90102-H","article-title":"Oxidation of phenolic compounds by ligninase","volume":"13","author":"Harvey","year":"1990","journal-title":"J. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0014-5793(98)00512-2","article-title":"A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta","volume":"428","author":"Heinfling","year":"1998","journal-title":"FEBS Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"10324","DOI":"10.1074\/jbc.274.15.10324","article-title":"Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites","volume":"274","author":"Camarero","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jbiotec.2013.08.007","article-title":"The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase","volume":"168","author":"Carabajal","year":"2013","journal-title":"J. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2174\/2211550105666160330205138","article-title":"Improved efficiency in screening for lignin-modifying peroxidases and laccases of basidiomycetes","volume":"6","author":"Kinnunen","year":"2016","journal-title":"Curr. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.15376\/biores.5.4.2362-2373","article-title":"Wheat straw conversion by enzymatic system of Ganoderma lucidum","volume":"5","author":"Kukavica","year":"2010","journal-title":"BioResources"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s12223-008-0045-7","article-title":"Degradation of PAHs by ligninolytic enzymes of Irpex lacteus","volume":"53","author":"Cajthaml","year":"2008","journal-title":"Folia Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5224","DOI":"10.15376\/biores.6.4.5224-5259","article-title":"Biological pretreatment of lignocelluloses with white-rot fungi and its applications: A review","volume":"6","author":"Isroi","year":"2011","journal-title":"BioResources"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1111\/jam.13717","article-title":"Inhibition and kinetic studies of cellulose and hemicellulose degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds","volume":"124","author":"Surendran","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"126443","DOI":"10.1016\/j.biortech.2021.126443","article-title":"Fungal biorefinery for sustainable resource recovery from waste","volume":"345","author":"Chatterjee","year":"2022","journal-title":"Bioresour. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2841","DOI":"10.1002\/jsfa.6118","article-title":"Performance of wood-rotting fungi-based enzymes on enzymic saccharification of rice straw","volume":"93","author":"Tsujiyama","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Banerjee, R., Chintagunta, A.D., and Ray, S. (2019). Laccase mediated delignification of pineapple leaf waste: An ecofriendly sustainable attempt towards valorization. BMC Chem., 13.","DOI":"10.1186\/s13065-019-0576-9"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"131687","DOI":"10.1016\/j.chemosphere.2021.131687","article-title":"Jute sticks biomass delignification through laccase-mediator system for enhanced saccharification and sustainable release of fermentable sugar","volume":"286","author":"Suman","year":"2022","journal-title":"Chemosphere"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7500","DOI":"10.1016\/j.biortech.2011.05.027","article-title":"Fungal pretreatment: An alternative in second-generation ethanol from wheat straw","volume":"102","author":"Prieto","year":"2011","journal-title":"Bioresour. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1039\/D1GC00738F","article-title":"Eco-friendly additives in acidic pretreatment to boost enzymatic saccharification of hardwood for sustainable biorefinery applications","volume":"23","author":"Chu","year":"2021","journal-title":"Green Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"125655","DOI":"10.1016\/j.biortech.2021.125655","article-title":"Enzymatic bioconversion process of lignin: Mechanisms, reactions and kinetics","volume":"340","author":"Cajnko","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"417","DOI":"10.18331\/BRJ2016.3.2.7","article-title":"Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain","volume":"3","author":"Wilkinson","year":"2016","journal-title":"Biofuel Res. J."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/5\/2572\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:25:59Z","timestamp":1760135159000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/5\/2572"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,23]]},"references-count":45,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["ijerph19052572"],"URL":"https:\/\/doi.org\/10.3390\/ijerph19052572","relation":{},"ISSN":["1660-4601"],"issn-type":[{"type":"electronic","value":"1660-4601"}],"subject":[],"published":{"date-parts":[[2022,2,23]]}}}