{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T08:31:07Z","timestamp":1778229067734,"version":"3.51.4"},"reference-count":50,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T00:00:00Z","timestamp":1337299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This paper describes the physiochemical, optical and biological activity of chitosan-chromone derivative. The chitosan-chromone derivative gels were prepared by reacting chitosan with chromone-3-carbaldehyde, followed by solvent exchange, filtration and drying by evaporation. The identity of Schiff base was confirmed by UV-Vis absorption spectroscopy and Fourier-transform infrared (FTIR) spectroscopy. The chitosan-chromone derivative was evaluated by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), photoluminescence (PL) and circular dichroism (CD). The CD spectrum showed the chitosan-chromone derivative had a secondary helical structure. Microbiological screening results demonstrated the chitosan-chromone derivative had antimicrobial activity against Escherichia coli bacteria. The chitosan-chromone derivative did not have any adverse effect on the cellular proliferation of mouse embryonic fibroblasts (MEF) and did not lead to cellular toxicity in MEFs. These results suggest that the chitosan-chromone derivative gels may open a new perspective in biomedical applications.<\/jats:p>","DOI":"10.3390\/ijms13056102","type":"journal-article","created":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T11:26:32Z","timestamp":1337340392000},"page":"6102-6116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":425,"title":["Physiochemical, Optical and Biological Activity of Chitosan-Chromone Derivative for Biomedical Applications"],"prefix":"10.3390","volume":"13","author":[{"given":"Santosh","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Textile Engineering, Konkuk University, Seoul 143-701, Korea"}]},{"given":"Joonseok","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Textile Engineering, Konkuk University, Seoul 143-701, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2012,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2894","DOI":"10.1021\/bm200423f","article-title":"Synthesis of multiresponsive and dynamic chitosan-based hydrogels for controlled release of bioactive molecules","volume":"12","author":"Zhang","year":"2011","journal-title":"Biomacromolecules"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1002\/app.31385","article-title":"Preparation, characterization, and optical properties of a chitosan-anthraldehyde crosslinkable film","volume":"115","author":"Kumar","year":"2010","journal-title":"J. Appl. Polym. Sci"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.carbpol.2009.12.033","article-title":"Preparation and characterization of optical property of crosslinkable film of chitosan with 2-thiophenecarboxaldehyde","volume":"80","author":"Kumar","year":"2010","journal-title":"Carbohydr. Polym"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1021\/bm201380e","article-title":"Exploring N-imidazolyl-O-carboxymethyl chitosan for high performance gene delivery","volume":"13","author":"Shi","year":"2012","journal-title":"Biomacromolecules"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6655","DOI":"10.1016\/j.biomaterials.2011.05.062","article-title":"The modulation of platelet adhesion and activation by chitosan through plasma and extracellular matrix proteins","volume":"32","author":"Lord","year":"2011","journal-title":"Biomaterials"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.carbpol.2011.08.044","article-title":"Synthesis, characterization and in vitro cytocompatibility studies of chitin nanogels for biomedical applications","volume":"87","author":"Rejinold","year":"2012","journal-title":"Carbohydr. Polym"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/j.carbpol.2009.05.028","article-title":"Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N,O-carboxymethyl chitosan nanoparticles","volume":"78","author":"Anitha","year":"2009","journal-title":"Carbohydr. Polym"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.carbpol.2010.04.074","article-title":"Biomedical applications of chitin and chitosan based nanomaterials\u2014A short review","volume":"82","author":"Jayakumar","year":"2010","journal-title":"Carbohydr. 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Sci. Eng. C"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"B108","DOI":"10.1002\/adem.201080094","article-title":"Structure process property relationship of biominetic chitosan based nanocomposite scaffolds for tissue engineering: Biological, physicochemical, and mechanical functions","volume":"13","author":"Maganti","year":"2011","journal-title":"Adv. Eng. Mater"},{"key":"ref_16","first-page":"1062","article-title":"Chitosan as a scaffold matrix in tissue engineering","volume":"34","author":"Misra","year":"2008","journal-title":"Mater. Sci. Technol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1016\/j.actbio.2008.02.002","article-title":"A stimulus-responsive magnetic nanoparticle drug carrier: Magnetite encapsulated by chitosan-grafted-copolymer","volume":"4","author":"Yuan","year":"2008","journal-title":"Acta Biomater"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1016\/j.actbio.2009.08.027","article-title":"Controlled and extended drug release behavior of chitosan-based nanoparticle carrier","volume":"6","author":"Yuan","year":"2010","journal-title":"Acta Biomater"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/j.msec.2011.04.010","article-title":"Controlled release of drug from folate-decorated and graphene mediated drug delivery system: Synthesis, loading efficiency, and drug release response","volume":"31","author":"Depan","year":"2011","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1016\/S0142-9612(03)00026-7","article-title":"Implantable applications of chitin and chitosan","volume":"24","author":"Khor","year":"2003","journal-title":"Biomaterials"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1016\/0142-9612(94)90093-0","article-title":"Stimulatory effect on bone formation exerted by a modified chitosan","volume":"15","author":"Muzzarelli","year":"1994","journal-title":"Biomaterials"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1016\/0142-9612(96)87284-X","article-title":"Novel approach to fabricate porous sponges of poly (D,L-lactic-co-glycolic acid) without the use of organic solvents","volume":"17","author":"Mooney","year":"1996","journal-title":"Biomaterials"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Muzzarelli, R., Jeuniaux, C., and Gooday, G.W. (1986). Chitin in Nature and Technology, Plenum Press.","DOI":"10.1007\/978-1-4613-2167-5"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.ijbiomac.2011.05.017","article-title":"A physiocochemical and biological study of novel chitosan-chloroquinoline derivative for biomedical applications","volume":"49","author":"Kumar","year":"2011","journal-title":"Int. J. Biol. Macromol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1080\/10601320903256539","article-title":"Preparation, characterization and optical property of chitosan-phenothiazine derivative by microwave assisted synthesis","volume":"46","author":"Kumar","year":"2009","journal-title":"J. Macromol. Sci. Pure Appl. Chem"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.biotechadv.2009.11.001","article-title":"Novel chitin and chitosan nanofibers in biomedical applications","volume":"79","author":"Jayakumar","year":"2010","journal-title":"Biotechnol. Adv"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4288","DOI":"10.1016\/j.ejmech.2010.06.030","article-title":"Synthesis and anticancer activity of chromone-based analogs of lavendustin A","volume":"45","author":"Nam","year":"2010","journal-title":"Eur. J. Med. Chem"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0968-0896(99)00282-5","article-title":"Synthesis and biological activity of some rigid analogues of flavones-8-acetic acid","volume":"8","author":"Valenti","year":"2000","journal-title":"Bioorg. Med. Chem"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1021\/np990538m","article-title":"Cytotoxic biflavonoids from Selaginella delicatula","volume":"63","author":"Lin","year":"2000","journal-title":"J. Nat. Prod"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1021\/np000317c","article-title":"Cytotoxic flavonoids with isoprenoid groups from morus mongolica","volume":"64","author":"Shi","year":"2001","journal-title":"J. Nat. Prod"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/S0960-894X(00)00110-4","article-title":"A convenient extension of the Wessly-Moser rearrangement for the synthesis of substituted alkylaminoflavones as neuroprotective agents in vitro","volume":"10","author":"Larget","year":"2000","journal-title":"Bioorg. Med. Chem. Lett"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1021\/np000175m","article-title":"HIV-inhibitory prenylated xanthones and flavones from Maclura tinctoria","volume":"63","author":"Groweiss","year":"2000","journal-title":"J. Nat. Prod"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3202","DOI":"10.1016\/j.bmcl.2011.04.055","article-title":"2-Arylmethylaminomethyl-5,6-dihydroxychromone derivatives with selective anti-HCV activity","volume":"21","author":"Park","year":"2011","journal-title":"Bioorg. Med. Chem. Lett"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"6678","DOI":"10.1016\/j.bmc.2010.07.065","article-title":"Anti-AIDS agents 79. Design, synthesis, molecular modelling and structure-activity relationships of novel dicamphanoyl-2\u2032,2\u2032-dimethyldihydropyranochromone (DCP) analogs as potent anti-HIV agents","volume":"18","author":"Zhou","year":"2010","journal-title":"Bioorg. Med. Chem"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1021\/np000054m","article-title":"New antimicrobial flavones from Physena madagascariensis","volume":"63","author":"Deng","year":"2000","journal-title":"J. Nat. Prod"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1021\/np000010d","article-title":"Antigiardial activity of isoflavones from Dalbergia frutescens bark","volume":"63","author":"Khan","year":"2000","journal-title":"J. Nat. Prod"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/S0031-9422(96)00494-3","article-title":"Further chromones from Eriosema tuberosum","volume":"43","author":"Ma","year":"1996","journal-title":"Phytochemistry"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1021\/np9904509","article-title":"Flavonoids as antioxidants","volume":"63","author":"Pietta","year":"2000","journal-title":"J. Nat. Prod"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4625","DOI":"10.1016\/j.bmc.2010.05.028","article-title":"Design and synthesis of novel hydroxyalkylaminomethylchromones for their IL-5 inhibitory activity","volume":"18","author":"Thanigaimalai","year":"2010","journal-title":"Bioorg. Med. Chem"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.1016\/j.ejmech.2009.01.034","article-title":"Synthesis of 2-styrylchromones as a novel class of antiproliferative agents targeting carcinoma cells","volume":"44","author":"Shaw","year":"2009","journal-title":"Eur. J. Med. Chem"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2952","DOI":"10.1016\/j.bmc.2007.02.004","article-title":"A chromone analog inhibits TNF-\u03b1 induced expression of cell adhesion molecules on human endothelial cells via blocking NF-\u03baB activation","volume":"15","author":"Kumar","year":"2007","journal-title":"Bioorg. Med. Chem"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1002\/pi.2333","article-title":"Chemical modification of chitosan: Synthesis and biological activity of new heterocyclic chitosan derivatives","volume":"57","author":"Badawy","year":"2008","journal-title":"Polym. Int"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.ijbiomac.2009.08.002","article-title":"Preparation and characterization of N-heterocyclic chitosan derivative based gels for biomedical applications","volume":"45","author":"Kumar","year":"2009","journal-title":"Int. J. Biol. Macromol"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.ijbiomac.2012.01.015","article-title":"A new chitosan-thymine conjugate: Synthesis, characterization and biological activity","volume":"50","author":"Kumar","year":"2012","journal-title":"Int. J. Biol. Macromol"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/j.reactfunctpolym.2008.06.015","article-title":"Synthesis and properties of a novel crosslinked chitosan resin modified by L-lysine","volume":"68","author":"Xiao","year":"2008","journal-title":"React. Funct. Polym"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0144-8617(98)00126-X","article-title":"Chitosan staple fibers and their chemical modification with some aldehydes","volume":"38","author":"Hirano","year":"1999","journal-title":"Carbhydr. Polym"},{"key":"ref_47","first-page":"227","article-title":"Synthesis and characterization of Schiff bases from chitosan","volume":"60","author":"Santos","year":"2005","journal-title":"Carbohydr. Polym"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1021\/ma00245a001","article-title":"Helical configuration of poly (iminomethylenes). Synthesis and CD spectra of polymers derived from optically active isocyanides","volume":"16","author":"Nolte","year":"1983","journal-title":"Macromolecules"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1351\/pac200274112021","article-title":"Hierarchial transfer of stereochemical information in synthetic macromolecules","volume":"74","author":"Cornelissen","year":"2002","journal-title":"Pure Appl. Chem"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1016\/j.ejmech.2010.02.041","article-title":"Synthesis and evaluation of novel chromone analogs for their inhibitory activity against interleukin-5","volume":"45","author":"Thanigaimalai","year":"2010","journal-title":"Eur. J. Med. 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