{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T15:08:03Z","timestamp":1776524883384,"version":"3.51.2"},"reference-count":27,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2014,10,28]],"date-time":"2014-10-28T00:00:00Z","timestamp":1414454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>  Purified isothiocyanates from cruciferous plants (Brassicacea, Syn. Cruciferae) plants were evaluated against 15 isolates of methicillin-resistant S. aureus isolated from diabetic foot-ulcer patients aiming the study of the potential usage of allyl-isothiocyanate, benzyl-isothiocyanate and 2-phenylethyl-isothiocyanate against this important bacteria.  Disc diffusion and minimum inhibitory concentration methods were used to access the antimicrobial activity. The index (Ia) and rate (Ra) of the antibacterial activity for each compound were calculated. The results showed a highly dose-dependent compound and chemical structure antibacterial effectiveness. The results showed a strong relation  between the chemical structure of isothiocyanates and its antibacterial effectiveness. The  benzyl-isothiocyanate was the most effective with a minimum inhibitory concentration varying between 2.9 and 110 \u00b5g\u00b7mL\u22121 with an antibacterial activity rate up to 87%. Moreover, their antibacterial activity was mainly bactericidal. This study provides scientific evidence that isothiocyanates have an interesting biological value and must be considered as an important tool to be used against MRSA.<\/jats:p>","DOI":"10.3390\/ijms151119552","type":"journal-article","created":{"date-parts":[[2014,10,28]],"date-time":"2014-10-28T10:25:07Z","timestamp":1414491907000},"page":"19552-19561","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["Antimicrobial Activity of Isothiocyanates from Cruciferous Plants against Methicillin-Resistant Staphylococcus aureus (MRSA)"],"prefix":"10.3390","volume":"15","author":[{"given":"Carla","family":"Dias","sequence":"first","affiliation":[{"name":"Animal and Veterinary Research Centre, CECAV, University of Tr\u00e1s-os-Montes and Alto Douro, UTAD, Quinta de Prados, Vila Real 5000801, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6291-8413","authenticated-orcid":false,"given":"Alfredo","family":"Aires","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB, University of Tr\u00e1s-os-Montes and Alto Douro, UTAD, Quinta de Prados, Vila Real 5000801, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7492-4965","authenticated-orcid":false,"given":"Maria","family":"Saavedra","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre, CECAV, University of Tr\u00e1s-os-Montes and Alto Douro, UTAD, Quinta de Prados, Vila Real 5000801, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2014,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1016\/j.jemermed.2008.11.021","article-title":"Mastitis and methicillin-resistant Staphylococcus aureus (MRSA): The calm before the storm?","volume":"38","author":"Schoenfeld","year":"2010","journal-title":"J. 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