{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:31:28Z","timestamp":1773923488533,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:00:00Z","timestamp":1493769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Geopropolis is a resin mixed with mud, produced only by stingless bees. Despite being popularly known for its medicinal properties, few scientific studies have proven its biological activities. In this context, the objective of this study was to determine the chemical composition and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of the Melipona orbignyi geopropolis. The hydroalcoholic extract of geopropolis (HEGP) was prepared and its chemical composition determined by high performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC-DAD-MS). The antioxidant activity was determined by the capture of free radicals and inhibition of lipid peroxidation in human erythrocytes. The anti-inflammatory activity was evaluated by the inhibition of the hyaluronidase enzyme and the antimutagenic action was investigated in Saccharomyces cerevisiae colonies. The antimicrobial activities were determined against bacteria and yeasts, isolated from reference strains and hospital origin. The chemical composition of HEGP included flavonoids, derivatives of glycosylated phenolic acids and terpenoids. HEGP showed high antioxidant activity, it inhibited the activity of the inflammatory enzyme hyaluronidase and reduced the mutagenic effects in S. cerevisiae. In relation to the antimicrobial activity, it promoted the death of all microorganisms evaluated. In conclusion, this study reveals for the first time the chemical composition of the HEGP of M. orbignyi and demonstrates its pharmacological properties.<\/jats:p>","DOI":"10.3390\/ijms18050953","type":"journal-article","created":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T12:24:47Z","timestamp":1493814287000},"page":"953","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis from the Stingless Bee Melipona orbignyi"],"prefix":"10.3390","volume":"18","author":[{"given":"Helder","family":"Santos","sequence":"first","affiliation":[{"name":"Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil"}]},{"given":"Jaqueline","family":"Campos","sequence":"additional","affiliation":[{"name":"Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil"}]},{"given":"Cintia","family":"Santos","sequence":"additional","affiliation":[{"name":"Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil"}]},{"given":"Jos\u00e9","family":"Balestieri","sequence":"additional","affiliation":[{"name":"Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil"}]},{"given":"Denise","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universit\u00e1ria, 79070-900 Campo Grande, MS, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1231-9441","authenticated-orcid":false,"given":"Carlos","family":"Carollo","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universit\u00e1ria, 79070-900 Campo Grande, MS, Brazil"}]},{"given":"Kely","family":"De Picoli Souza","sequence":"additional","affiliation":[{"name":"Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil"}]},{"given":"Leticia","family":"Estevinho","sequence":"additional","affiliation":[{"name":"Polytechnic Institute of Bragan\u00e7a, Agricultural College of Bragan\u00e7a, Campus Santa Apol\u00f3nia, E 5301-855 Bragan\u00e7a, Portugal"},{"name":"Centre of Molecular and Environmental Biology, Biology Department, Minho University, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Edson","family":"Dos Santos","sequence":"additional","affiliation":[{"name":"Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2017,5,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1590\/S0103-50532008000300022","article-title":"Flavonoids and triterpenes from the nest of the stingless bee Trigona spinipes","volume":"19","author":"Freitas","year":"2008","journal-title":"J. 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