{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T16:19:00Z","timestamp":1774541940872,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,4,4]],"date-time":"2018-04-04T00:00:00Z","timestamp":1522800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In marine fish, high epithelial intestinal HCO3\u2212 secretion generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. The present study was designed to expose the putative role for calcium and the calcium-sensing receptor (CaSR) in the regulation of HCO3\u2212 secretion in the intestine of the sea bream (Sparus aurata L.). Effects on the expression of the CaSR in the intestine were evaluated by qPCR and an increase was observed in the anterior intestine in fed fish compared with unfed fish and with different regions of intestine. CaSR expression reflected intestinal fluid calcium concentration. In addition, anterior intestine tissue was mounted in Ussing chambers to test the putative regulation of HCO3\u2212 secretion in vitro using the anterior intestine. HCO3\u2212 secretion was sensitive to varying calcium levels in luminal saline and to calcimimetic compounds known to activate\/block the CaSR i.e., R 568 and NPS-2143. Subsequent experiments were performed in intestinal sacs to measure water absorption and the sensitivity of water absorption to varying luminal levels of calcium and calcimimetics were exposed as well. It appears, that CaSR mediates HCO3\u2212 secretion and water absorption in marine fish as shown by responsiveness to calcium levels and calcimimetic compounds.<\/jats:p>","DOI":"10.3390\/ijms19041072","type":"journal-article","created":{"date-parts":[[2018,4,4]],"date-time":"2018-04-04T03:43:51Z","timestamp":1522813431000},"page":"1072","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Regulation of Bicarbonate Secretion in Marine Fish Intestine by the Calcium-Sensing Receptor"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6648-5122","authenticated-orcid":false,"given":"S\u00edlvia F.","family":"Greg\u00f3rio","sequence":"first","affiliation":[{"name":"Centre of Marine Sciences (CCMar), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Juan","family":"Fuentes","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences (CCMar), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/BF00689177","article-title":"Osmotic and ionic regulation in the cod (Gadus callarias L.)","volume":"124","author":"Fletcher","year":"1978","journal-title":"J. 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