{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T18:16:03Z","timestamp":1778177763525,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,4,13]],"date-time":"2018-04-13T00:00:00Z","timestamp":1523577600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT-MCTES","award":["(3599-PPCDT) PTDC\/DTP-FTO\/1981\/2014 \u2013 POCI-01-0145-FEDER-016581"],"award-info":[{"award-number":["(3599-PPCDT) PTDC\/DTP-FTO\/1981\/2014 \u2013 POCI-01-0145-FEDER-016581"]}]},{"name":"FCT-MCTES","award":["RECI\/BBB-BEP\/0124\/2012"],"award-info":[{"award-number":["RECI\/BBB-BEP\/0124\/2012"]}]},{"name":"FCT-MCTES","award":["PD\/BD\/114046\/2015"],"award-info":[{"award-number":["PD\/BD\/114046\/2015"]}]},{"name":"FCT-MCTES","award":["SFRH\/BD\/96189\/2013"],"award-info":[{"award-number":["SFRH\/BD\/96189\/2013"]}]},{"name":"FCT_MCTES","award":["PT2020 UID\/MULTI\/04378\/2013"],"award-info":[{"award-number":["PT2020 UID\/MULTI\/04378\/2013"]}]},{"name":"FEDER-COMPETE","award":["POCI\/01\/0145\/FEDER\/007728"],"award-info":[{"award-number":["POCI\/01\/0145\/FEDER\/007728"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The p53 tumor suppressor is widely found to be mutated in human cancer. This protein is regarded as a molecular hub regulating different cell responses, namely cell death. Compelling data have demonstrated that the impairment of p53 activity correlates with tumor development and maintenance. For these reasons, the reactivation of p53 function is regarded as a promising strategy to halt cancer. In the present work, the recombinant mutant p53R280K DNA binding domain (DBD) was produced for the first time, and its crystal structure was determined in the absence of DNA to a resolution of 2.0 \u00c5. The solved structure contains four molecules in the asymmetric unit, four zinc(II) ions, and 336 water molecules. The structure was compared with the wild-type p53 DBD structure, isolated and in complex with DNA. These comparisons contributed to a deeper understanding of the mutant p53R280K structure, as well as the loss of DNA binding related to halted transcriptional activity. The structural information derived may also contribute to the rational design of mutant p53 reactivating molecules with potential application in cancer treatment.<\/jats:p>","DOI":"10.3390\/ijms19041184","type":"journal-article","created":{"date-parts":[[2018,4,13]],"date-time":"2018-04-13T14:38:14Z","timestamp":1523630294000},"page":"1184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Crystal Structure of the R280K Mutant of Human p53 Explains the Loss of DNA Binding"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9378-6043","authenticated-orcid":false,"given":"Ana","family":"Gomes","sequence":"first","affiliation":[{"name":"LAQV-REQUIMTE, Laborato\u0301rio de Microbiologia, Departamento de Cie\u0302ncias Biolo\u0301gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"given":"Filipa","family":"Trov\u00e3o","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Benedita","family":"Andrade Pinheiro","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Filipe","family":"Freire","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9086-2303","authenticated-orcid":false,"given":"Sara","family":"Gomes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Laborato\u0301rio de Microbiologia, Departamento de Cie\u0302ncias Biolo\u0301gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"given":"Carla","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Luc\u00edlia","family":"Domingues","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3004-0543","authenticated-orcid":false,"given":"Maria","family":"Rom\u00e3o","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9531-4939","authenticated-orcid":false,"given":"Luc\u00edlia","family":"Saraiva","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Laborato\u0301rio de Microbiologia, Departamento de Cie\u0302ncias Biolo\u0301gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3824-0240","authenticated-orcid":false,"given":"Ana","family":"Carvalho","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.cell.2009.04.037","article-title":"Blinded by the light: The growing complexity of p53","volume":"137","author":"Vousden","year":"2009","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/cdd.2017.185","article-title":"Mutant p53 partners in crime","volume":"25","author":"Kim","year":"2018","journal-title":"Cell Death Differ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1146\/annurev-biochem-060815-014710","article-title":"The p53 pathway: Origins, inactivation in cancer, and emerging therapeutic approaches","volume":"85","author":"Joerger","year":"2016","journal-title":"Annu. 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