{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:24:32Z","timestamp":1772252672593,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T00:00:00Z","timestamp":1532390400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005004","name":"Ekonomiaren Garapen eta Lehiakortasun Saila, Eusko Jaurlaritza","doi-asserted-by":"publisher","award":["ELKARTEK and HAZITEK"],"award-info":[{"award-number":["ELKARTEK and HAZITEK"]}],"id":[{"id":"10.13039\/501100005004","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["MAT2016-76039-C4-3-R (AEI\/FEDER, UE)"],"award-info":[{"award-number":["MAT2016-76039-C4-3-R (AEI\/FEDER, UE)"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/FIS\/04650\/2013, UID\/QUI\/00686\/2013 and UID\/QUI\/0686\/2016 and project PTDC\/EEI-SII\/5582\/2014 and project POCI-01-0145-FEDER-028237"],"award-info":[{"award-number":["UID\/FIS\/04650\/2013, UID\/QUI\/00686\/2013 and UID\/QUI\/0686\/2016 and project PTDC\/EEI-SII\/5582\/2014 and project POCI-01-0145-FEDER-028237"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Polymer-based piezoelectric biomaterials have already proven their relevance for tissue engineering applications. Furthermore, the morphology of the scaffolds plays also an important role in cell proliferation and differentiation. The present work reports on poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV), a biocompatible, biodegradable, and piezoelectric biopolymer that has been processed in different morphologies, including films, fibers, microspheres, and 3D scaffolds. The corresponding magnetically active PHBV-based composites were also produced. The effect of the morphology on physico-chemical, thermal, magnetic, and mechanical properties of pristine and composite samples was evaluated, as well as their cytotoxicity. It was observed that the morphology does not strongly affect the properties of the pristine samples but the introduction of cobalt ferrites induces changes in the degree of crystallinity that could affect the applicability of prepared biomaterials. Young\u2019s modulus is dependent of the morphology and also increases with the addition of cobalt ferrites. Both pristine and PHBV\/cobalt ferrite composite samples are not cytotoxic, indicating their suitability for tissue engineering applications.<\/jats:p>","DOI":"10.3390\/ijms19082149","type":"journal-article","created":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T11:51:38Z","timestamp":1532433098000},"page":"2149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Tailored Biodegradable and Electroactive Poly(Hydroxybutyrate-Co-Hydroxyvalerate) Based Morphologies for Tissue Engineering Applications"],"prefix":"10.3390","volume":"19","author":[{"given":"Lu\u00eds","family":"Amaro","sequence":"first","affiliation":[{"name":"Center\/Department of Physics, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Daniela M.","family":"Correia","sequence":"additional","affiliation":[{"name":"Center\/Department of Chemistry, Universidade de Tr\u00e1s-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal"},{"name":"BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV\/EHU Science Park, 48940 Leioa, Spain"}]},{"given":"Teresa","family":"Marques-Almeida","sequence":"additional","affiliation":[{"name":"Center\/Department of Physics, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Pedro M.","family":"Martins","sequence":"additional","affiliation":[{"name":"Center\/Department of Physics, Universidade do Minho, 4710-057 Braga, Portugal"},{"name":"Center\/Department of Chemistry, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Leyre","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV\/EHU Science Park, 48940 Leioa, Spain"},{"name":"Macromolecular Chemistry Research Group (labquimac), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}]},{"given":"Jos\u00e9 L.","family":"Vilas","sequence":"additional","affiliation":[{"name":"BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV\/EHU Science Park, 48940 Leioa, Spain"},{"name":"Macromolecular Chemistry Research Group (labquimac), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4680-0834","authenticated-orcid":false,"given":"Gabriela","family":"Botelho","sequence":"additional","affiliation":[{"name":"Center\/Department of Chemistry, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6791-7620","authenticated-orcid":false,"given":"Senentxu","family":"Lanceros-Mendez","sequence":"additional","affiliation":[{"name":"BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV\/EHU Science Park, 48940 Leioa, Spain"},{"name":"IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9120-4847","authenticated-orcid":false,"given":"Clarisse","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Center\/Department of Physics, Universidade do Minho, 4710-057 Braga, Portugal"},{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.addr.2007.08.041","article-title":"Biomimetic materials for tissue engineering","volume":"60","author":"Ma","year":"2008","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4353","DOI":"10.1016\/S0142-9612(03)00339-9","article-title":"Biomimetic materials for tissue engineering","volume":"24","author":"Shin","year":"2003","journal-title":"Biomaterials"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1469-7580.2008.00878.x","article-title":"Tissue engineering: Strategies, stem cells and scaffolds","volume":"213","author":"Howard","year":"2008","journal-title":"J. Anat."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3413","DOI":"10.1016\/j.biomaterials.2006.01.039","article-title":"Biodegradable and bioactive porous polymer\/inorganic composite scaffolds for bone tissue engineering","volume":"27","author":"Rezwan","year":"2006","journal-title":"Biomaterials"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S1369-7021(11)70058-X","article-title":"Biomaterials & scaffolds for tissue engineering","volume":"14","year":"2011","journal-title":"Mater. Today"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1089\/107632701753337726","article-title":"Enhancement of chondrogenic differentiation of human articular chondrocytes by biodegradable polymers","volume":"7","author":"Rahman","year":"2001","journal-title":"Tissue Eng."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1002\/jbm.a.35234","article-title":"Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)","volume":"103","author":"Parssinen","year":"2015","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0142-9612(91)90072-I","article-title":"In vitro and in vivo evaluation of polyhydroxybutyrate and of polyhydroxybutyrate reinforced with hydroxyapatite","volume":"12","author":"Doyle","year":"1991","journal-title":"Biomaterials"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1089\/ten.2004.10.1234","article-title":"In vivo tissue engineering of bone using poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) and collagen scaffolds","volume":"10","author":"Kose","year":"2004","journal-title":"Tissue Eng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0141-8130(86)90056-5","article-title":"Piezoelectric properties of poly-\u03b2-hydroxybutyrate and copolymers of \u03b2-hydroxybutyrate and \u03b2-hydroxyvalerate","volume":"8","author":"Fukada","year":"1986","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.7567\/JJAPS.24S2.23","article-title":"Piezoelectric polymers\u2013materials and manufacture","volume":"24","author":"Ohigashi","year":"1985","journal-title":"Jpn. J. Appl. Phys."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"09LD14","DOI":"10.1143\/JJAP.51.09LD14","article-title":"Film sensor device fabricated by a piezoelectric poly(l-lactic acid) film","volume":"51","author":"Ando","year":"2012","journal-title":"Jpn. J. Appl. Phys."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11504","DOI":"10.1039\/c2ra21841k","article-title":"Enhanced proliferation of pre-osteoblastic cells by dynamic piezoelectric stimulation","volume":"2","author":"Ribeiro","year":"2012","journal-title":"RSC Adv."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"17938","DOI":"10.1039\/c3ra43499k","article-title":"Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering","volume":"3","author":"Martins","year":"2013","journal-title":"RSC Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.colsurfb.2015.08.043","article-title":"Piezoelectric polymers as biomaterials for tissue engineering applications","volume":"136","author":"Ribeiro","year":"2015","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6565","DOI":"10.1016\/j.biomaterials.2005.04.036","article-title":"The application of polyhydroxyalkanoates as tissue engineering materials","volume":"26","author":"Chen","year":"2005","journal-title":"Biomaterials"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/nprot.2017.157","article-title":"Electroactive poly(vinylidene fluoride)-based structures for advanced applications","volume":"13","author":"Ribeiro","year":"2018","journal-title":"Nat. Protoc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.colsurfb.2015.12.055","article-title":"Proving the suitability of magnetoelectric stimuli for tissue engineering applications","volume":"140","author":"Ribeiro","year":"2016","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3371","DOI":"10.1002\/adfm.201202780","article-title":"Polymer-based magnetoelectric materials","volume":"23","author":"Martins","year":"2013","journal-title":"Adv. Funct. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1080\/00150193.2014.923670","article-title":"Magnetoelectric laminate composite: Effect of piezoelectric layer on magnetoelectric properties","volume":"473","author":"Cho","year":"2014","journal-title":"Ferroelectrics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"286a","DOI":"10.1016\/j.bpj.2016.11.1549","article-title":"Magnetoelectric neural modulation","volume":"112","author":"Wickens","year":"2017","journal-title":"Biophys. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1557\/adv.2018.223","article-title":"Piezo-and magnetoelectric polymers as biomaterials for novel tissue engineering strategies","volume":"3","author":"Ribeiro","year":"2018","journal-title":"MRS Adv."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1557\/mrs.2015.195","article-title":"Multiferroic magnetoelectric nanostructures for novel device applications","volume":"40","author":"Hu","year":"2015","journal-title":"MRS Bull."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1016\/j.matdes.2015.12.043","article-title":"Strategies for the development of three dimensional scaffolds from piezoelectric poly(vinylidene fluoride)","volume":"92","author":"Correia","year":"2016","journal-title":"Mater. Des."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1016\/S0014-3057(03)00114-9","article-title":"Quantitative FTIR study of PHBV\/bisphenol a blends","volume":"39","author":"Fei","year":"2003","journal-title":"Eur. Polym. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1002\/app.25348","article-title":"Thermal and spectroscopic characterization of microbial poly(3-hydroxybutyrate) submicrometer fibers prepared by electrospinning","volume":"103","author":"Kim","year":"2007","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5695","DOI":"10.1007\/s10853-016-9872-0","article-title":"Preparation and characterization of electrospun phbv\/peo mats: The role of solvent and peo component","volume":"51","author":"Xu","year":"2016","journal-title":"J. Mater. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/S1002-0071(12)60086-0","article-title":"Cellulose nanocrystals as green fillers to improve crystallization and hydrophilic property of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)","volume":"21","author":"Yu","year":"2011","journal-title":"Prog. Nat. Sci. Mater. Int."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1002\/jbm.a.35368","article-title":"Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells","volume":"103","author":"Ribeiro","year":"2014","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jcis.2016.05.012","article-title":"Processing and size range separation of pristine and magnetic poly(l-lactic acid) based microspheres for biomedical applications","volume":"476","author":"Correia","year":"2016","journal-title":"J. Colloid Interface Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1080\/00914037.2010.531809","article-title":"Polyhydroxybutyrate (phb) scaffolds as a model for nerve tissue engineering application: Fabrication and in vitro assay","volume":"60","author":"Khorasani","year":"2011","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/S0142-9418(01)00142-8","article-title":"Correlation between degree of crystallinity, morphology, glass temperature, mechanical properties and biodegradation of poly (3-hydroxyalkanoate) phas and their blends","volume":"21","author":"Schnabel","year":"2002","journal-title":"Polym. Test."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1080\/00222340902837527","article-title":"A to \u03b2 phase transformation and microestructural changes of pvdf films induced by uniaxial stretch","volume":"48","author":"Sencadas","year":"2009","journal-title":"J. Macromol. Sci. Part B Phys."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.compositesb.2017.12.046","article-title":"Silk fibroin-magnetic hybrid composite electrospun fibers for tissue engineering applications","volume":"141","author":"Correia","year":"2018","journal-title":"Compos. Part B Eng."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"35852","DOI":"10.1039\/C5RA04409J","article-title":"Development of magnetoelectric cofe2o4\/poly(vinylidene fluoride) microspheres","volume":"5","author":"Goncalves","year":"2015","journal-title":"RSC Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.compositesb.2017.11.065","article-title":"Relation between fiber orientation and mechanical properties of nano-engineered poly(vinylidene fluoride) electrospun composite fiber mats","volume":"139","author":"Maciel","year":"2018","journal-title":"Compos. Part B Eng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.1039\/c2ce06654h","article-title":"On the origin of the electroactive poly(vinylidene fluoride) \u03b2-phase nucleation by ferrite nanoparticles via surface electrostatic interactions","volume":"14","author":"Martins","year":"2012","journal-title":"CrystEngComm"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1080\/00222348.2015.1011063","article-title":"Polymer-nanoparticle composites composed of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and coated silver nanoparticles","volume":"54","author":"Min","year":"2015","journal-title":"J. Macromol. Sci. Part B"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1590\/S1516-14392011005000046","article-title":"Morphology and thermal properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)\/attapulgite nanocomposites","volume":"14","author":"Arruda","year":"2011","journal-title":"Mater. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1007\/s10973-016-5260-x","article-title":"Effect of side substituents on thermal stability of the modified chitosan and its nanocomposites with magnetite","volume":"124","author":"Kaczmarek","year":"2016","journal-title":"J. Therm. Anal. Calorim."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-3-642-34243-1_1","article-title":"Contact angle and wetting properties","volume":"Volume 51","author":"Yuan","year":"2013","journal-title":"Springer Series in Surface Sciences"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5818","DOI":"10.1039\/c2sm25557j","article-title":"Influence of crystallinity and fiber orientation on hydrophobicity and biological response of poly(l-lactide) electrospun mats","volume":"8","author":"Areias","year":"2012","journal-title":"Soft Matter"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1021\/ar040224c","article-title":"Bioinspired surfaces with special wettability","volume":"38","author":"Sun","year":"2005","journal-title":"Acc. Chem. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.apsusc.2014.05.187","article-title":"Effect of filler dispersion and dispersion method on the piezoelectric and magnetoelectric response of CoFe2O4\/P(VDF-TrFE) nanocomposites","volume":"313","author":"Martins","year":"2014","journal-title":"Appl. Surf. Sci."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/8\/2149\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:13:56Z","timestamp":1760195636000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/8\/2149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,24]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2018,8]]}},"alternative-id":["ijms19082149"],"URL":"https:\/\/doi.org\/10.3390\/ijms19082149","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints201807.0021.v1","asserted-by":"object"}]},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,24]]}}}