{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T09:36:02Z","timestamp":1769679362880,"version":"3.49.0"},"reference-count":38,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:00:00Z","timestamp":1546905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Alentejo 2020","award":["OLEAVALOR-ALT20-03-0145-FEDER-000014"],"award-info":[{"award-number":["OLEAVALOR-ALT20-03-0145-FEDER-000014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Three different high performance liquid chromatography columns were accessed for phenolic compounds (PC) separation in the hydrophilic fraction of extra virgin olive oil (EVOO). Two fully porous C18 bonded silica phases and one partially porous biphenyl column were used. Biphenyl column allowed for an increase of more than 30% in peak capacity (nc), higher selectivity (\u03b1) (1.045), and improved retention (k), with a reduction of 22.1% in the retention time. The higher resolution (Rs) was obtained by using the biphenyl column, with a fair separation of oleuropein aglycone isomers (OAI) and a good identification of caffeic acid (CA). Tyrosol (T), hydroxytyrosol (HT), and dihydroxyphenyl glycol (DHPG) were also well separated and identified. Moreover, the method using a biphenyl column was fully validated according to the requirements for new methods. For all parameters, the method applying the biphenyl column proved to be a reliable, accurate, and robust tool for separation, identification, and quantification of the main PCs in EVOOs.<\/jats:p>","DOI":"10.3390\/ijms20010201","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T03:06:06Z","timestamp":1547003166000},"page":"201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Chromatographic Separation of Phenolic Compounds from Extra Virgin Olive Oil: Development and Validation of a New Method Based on a Biphenyl HPLC Column"],"prefix":"10.3390","volume":"20","author":[{"given":"Miguel D.","family":"Ferro","sequence":"first","affiliation":[{"name":"Centro de Biotecnologia Agr\u00edcola e Agro-Alimentar do Alentejo (CEBAL)\/Instituto Polit\u00e9cnico de Beja (IPBeja), 7801-908 Beja, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6749-7619","authenticated-orcid":false,"given":"S\u00f3nia A. O.","family":"Santos","sequence":"additional","affiliation":[{"name":"CICECO\u2014Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-8416","authenticated-orcid":false,"given":"Armando J. D.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"CICECO\u2014Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2223-7784","authenticated-orcid":false,"given":"Maria F.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia Agr\u00edcola e Agro-Alimentar do Alentejo (CEBAL)\/Instituto Polit\u00e9cnico de Beja (IPBeja), 7801-908 Beja, Portugal"},{"name":"Instituto de Ci\u00eancias Agr\u00e1rias e Ambientais Mediterr\u00e2nicas (ICAAM), Universidade de \u00c9vora, P\u00f3lo da Mitra, 7002-554 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Panel on Dietetic Products, Nutrition and Allergies (2011). 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